Restriction map for
pFN21AB8772
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# NO CUT ENZYMES ( 149 enzymes) #
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AatII Acc65I AccI AccII AccIII
AciI AclI AcyI AflIII AgeI
AhdI Alw44I AocI Aor51HI ApaI
ApaLI AscI Asp700I Asp718I AspEI
AspI AvaI AviII AvrII BalI
BanI BanII BbeI BcgI BciVI
BcnI BglI BglII BlnI BlpI
Bpu1102I BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspLU11I BspMI
BsrBI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstPI BstUI
BstXI BstZ17I Bsu36I CelII CfoI
Cfr10I ClaI CpoI DrdI DsaI
EaeI EagI Eam1105I EclXI Eco47III
Eco52I Eco81I EcoO65I EcoRI EcoRV
EcoT22I FauI FseI FspI HaeII
HapII HgaI HhaI Hin1I HinP1I
HindIII HpaII KasI KpnI KspI
MamI MfeI MluI MluNI MroI
MscI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI Psp1406I PstI PvuI
RsrII SacI SacII SalI SbfI
SexAI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SwaI Tth111I Van91I XbaI
XhoI XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 40 enzymes) #
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AflII 955 CTTAAG C^TTAAG
AlwNI 113 CAGTGGCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 1659 ATTAAT AT^TAAT
AsnI 1659 ATTAAT AT^TAAT
AspHI 1621 GAGCAC G[AT]GC[AT]^C
BamHI 969 GGATCC G^GATCC
BbrPI 905 CACGTG CAC^GTG
BbsI 1143 GTCTTC GAAGAC(2/6)
BbvI 1227 GCAGC GCAGC(8/12)
BfrI 955 CTTAAG C^TTAAG
BpuAI 1143 GTCTTC GAAGAC(2/6)
BsaAI 905 CACGTG [CT]AC^GT[GA]
BseRI 770 CTCCTC GAGGAG(10/8)
BsgI 1225 GTGCAG GTGCAG(16/14)
BsiHKAI 1621 GAGCAC G[AT]GC[AT]^C
BsmFI 13 GGGAC GGGAC(10/14)
BsrDI 172 GCAATG GCAATG(2/0)
BstAPI 717 GCAATCCTTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraI 1356 TTTAAA TTT^AAA
DraIII 1279 CACTATGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 1538 CCAAGG C^C[AT][AT]GG
Fnu4HI 1227 GCAGC GC^[ACGT]GC
HpaI 960 GTTAAC GTT^AAC
ItaI 1227 GCAGC GC^[ACGT]GC
MaeII 906 ACGT A^CGT
MspA1I 1228 CAGCTG C[AC]G^C[GT]G
MwoI 717 GCAATCCTTGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
PmaCI 905 CACGTG CAC^GTG
PmlI 905 CACGTG CAC^GTG
PshAI 255 GACTAGAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PshBI 1659 ATTAAT AT^TAAT
PvuII 1228 CAGCTG CAG^CTG
SapI 532 GAAGAGC GCTCTTC(1/4)
SfaNI 1007 GCATC GCATC(5/9)
SfcI 198 CTACAG C^T[GA][CT]AG
StuI 887 AGGCCT AGG^CCT
StyI 1538 CCAAGG C^C[AT][AT]GG
TaqI 783 TCGA T^CGA
TseI 1227 GCAGC G^C[AT]GC
TthHB8I 783 TCGA T^CGA
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# TWO CUT ENZYMES ( 21 enzymes) #
###################################
AcsI 1267 AAATTT [GA]^AATT[CT]
1503 AAATTT
AfaI 350 GTAC GT^AC
1420 GTAC
ApoI 1267 AAATTT [GA]^AATT[CT]
1503 AAATTT
BbvCI 40 GCTGAGG CCTCAGC(-5/-2)
118 GCTGAGG
BclI 230 TGATCA T^GATCA
1406 TGATCA
BmyI 1252 GGGCAC G[AGT]GC[ACT]^C
1621 GAGCAC
Bsp1286I 1252 GGGCAC G[AGT]GC[ACT]^C
1621 GAGCAC
BspHI 981 TCATGA T^CATGA
1044 TCATGA
DraII 403 AGGTCCT [GA]G^G[ACGT]CC[CT]
547 GGGTCCT
EcoNI 538 CCTAGTGTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
727 CCTCCAGAAGG
EcoO109I 403 AGGTCCT [GA]G^G[ACGT]CC[CT]
547 GGGTCCT
FbaI 230 TGATCA T^GATCA
1406 TGATCA
HincII 4 GTTGAC GT[CT]^[GA]AC
960 GTTAAC
HindII 4 GTTGAC GT[CT]^[GA]AC
960 GTTAAC
HphI 163 GGTGA GGTGA(8/7)
1313 TCACC
MslI 330 CATGCCTATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
471 CATTGGGATG
PpuMI 403 AGGTCCT [GA]G^G[AT]CC[CT]
547 GGGTCCT
RcaI 981 TCATGA T^CATGA
1044 TCATGA
RsaI 350 GTAC GT^AC
1420 GTAC
ScaI 349 AGTACT AGT^ACT
1419 AGTACT
XcmI 150 CCAGGCCAAAGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
730 CCAGAAGGAAACTGG
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# 3 OR MORE CUT ENZYMES ( 48 enzymes) #
#########################################
AluI AlwI AvaII BfaI BpmI
BsaI BsaJI BsiYI BslI BsmAI
BsrI BssKI BstF5I BstNI BstYI
Cac8I Cfr13I DdeI DpnII EarI
EcoRII FokI HaeIII HinfI Ksp632I
MaeI MaeIII MboI MboII MflI
MnlI MseI MvaI NdeII NlaIII
NlaIV PleI Sau3AI Sau96I ScrFI
SmlI SspI TfiI Tru9I Tsp45I
Tsp509I TspRI XhoII