(Circular) (Six-base) MAPSORT of: pFN21AB8772.seq Check: 1572 from: 1 to: 6379 pFN21AB8772 6379 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4189 278 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3722 1937 Size: 1785 4594 Acc65I G'GTAC_C Cuts at: 3700 3700 Size: 6379 AclI AA'CG_TT Cuts at: 1563 4506 4879 1563 Size: 2943 373 3063 Fragments arranged by size: 3063 2943 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2391 2589 4442 5826 6094 1632 Size: 759 198 1853 1384 268 1917 Fragments arranged by size: 1917 1853 1384 759 268 198 AflII C'TTAA_G Cuts at: 820 1017 2960 820 Size: 197 1943 4239 Fragments arranged by size: 4239 1943 197 AflIII A'CryG_T Cuts at: 1203 5278 6255 1203 Size: 4075 977 1327 Fragments arranged by size: 4075 1327 977 AgeI A'CCGG_T Cuts at: 5210 5210 Size: 6379 AhdI GACnn_n'nnGTC Cuts at: 5108 5108 Size: 6379 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6379 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2327 1184 Size: 1143 5236 AlwNI CAG_nnn'CTG Cuts at: 2123 4122 5694 2123 Size: 1999 1572 2808 Fragments arranged by size: 2808 1999 1572 ApaLI G'TGCA_C Cuts at: 4436 5592 4436 Size: 1156 5223 ApoI r'AATT_y Cuts at: 1493 3272 3508 3688 3914 4066 6175 1493 Size: 1779 236 180 226 152 2109 1697 Fragments arranged by size: 2109 1779 1697 236 226 180 152 AseI AT'TA_AT Cuts at: 160 1032 3665 4933 160 Size: 872 2633 1268 1606 Fragments arranged by size: 2633 1606 1268 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 AvaI C'yCGr_G Cuts at: 1870 1943 3704 1870 Size: 73 1761 4545 Fragments arranged by size: 4545 1761 73 BamHI G'GATC_C Cuts at: 2974 3709 4076 2974 Size: 735 367 5277 Fragments arranged by size: 5277 735 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3700 5155 618 Size: 325 217 627 1913 1455 1842 Fragments arranged by size: 1913 1842 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 3698 1391 Size: 2307 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3141 928 Size: 971 1242 4166 Fragments arranged by size: 4166 1242 971 BbvCI CC'TCA_GC Cuts at: 2046 2124 2046 Size: 78 6301 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4570 4604 1903 Size: 34 2633 34 3678 Fragments arranged by size: 3678 2633 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4271 5481 4271 Size: 1210 5169 BclI T'GATC_A Cuts at: 1548 2235 3411 1548 Size: 687 1176 4516 Fragments arranged by size: 4516 1176 687 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4990 136 Size: 107 122 71 631 605 3318 1525 Fragments arranged by size: 3318 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6374 6374 Size: 6379 BlpI GC'TnA_GC Cuts at: 3796 3796 Size: 6379 Bme1580I G_kGCm'C Cuts at: 1790 3261 4440 5596 1790 Size: 1471 1179 1156 2573 Fragments arranged by size: 2573 1471 1179 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6379 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2451 4110 5068 Size: 416 53 83 399 24 1034 1659 958 Cuts at: 5068 442 Size: 1753 Fragments arranged by size: 1753 1659 1034 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6379 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2553 2718 3135 5039 1239 Size: 1314 165 417 1904 2579 Fragments arranged by size: 2579 1904 1314 417 165 Bpu10I CC'TnA_GC Cuts at: 2046 2124 2046 Size: 78 6301 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3021 3283 4504 5369 5667 3021 Size: 262 1221 865 298 3733 Fragments arranged by size: 3733 1221 865 298 262 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2462 2570 2753 5042 882 Size: 617 963 108 183 2289 2219 Fragments arranged by size: 2289 2219 963 617 183 108 BsaAI yAC'GTr Cuts at: 493 1204 2912 4170 493 Size: 711 1708 1258 2702 Fragments arranged by size: 2702 1708 1258 711 BsaBI GATnn'nnATC Cuts at: 6373 6373 Size: 6379 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4186 4568 5203 275 Size: 53 83 186 936 2653 382 635 1451 Fragments arranged by size: 2653 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4812 5210 5484 5631 6042 4812 Size: 398 274 147 411 5149 Fragments arranged by size: 5149 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6154 Fragments arranged by size: 6154 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2766 1186 Size: 1580 4799 BseYI C'CCAG_C Cuts at: 5582 6078 5582 Size: 496 5883 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3251 1163 Size: 573 1515 4291 Fragments arranged by size: 4291 1515 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3829 4590 5618 1540 Size: 275 186 1828 761 1028 2301 Fragments arranged by size: 2301 1828 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3630 4440 4525 5596 3630 Size: 810 85 1071 4413 Fragments arranged by size: 4413 1071 810 85 BsmI GAATG_Cn' Cuts at: 3890 3983 3890 Size: 93 6286 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3261 3630 3698 4440 4525 5596 1391 Size: 399 1471 369 68 742 85 1071 2174 Fragments arranged by size: 2174 1471 1071 742 399 369 85 68 BspEI T'CCGG_A Cuts at: 6042 6042 Size: 6379 BspHI T'CATG_A Cuts at: 2986 3049 4268 5998 2986 Size: 63 1219 1730 3367 Fragments arranged by size: 3367 1730 1219 63 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3734 844 Size: 2890 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4266 1394 Size: 429 2443 3507 Fragments arranged by size: 3507 2443 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2184 4874 5048 65 Size: 1183 936 2690 174 1396 Fragments arranged by size: 2690 1396 1183 936 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5023 5210 1700 Size: 110 3213 187 2869 Fragments arranged by size: 3213 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6379 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6379 BssSI C'ACGA_G Cuts at: 4439 5451 4439 Size: 1012 5367 BstAPI GCAn_nnn'nTGC Cuts at: 794 2728 794 Size: 1934 4445 BstBI TT'CG_AA Cuts at: 5183 5183 Size: 6379 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6379 BstYI r'GATC_y Cuts at: 1972 2610 2802 2974 3709 4076 4461 4478 5930 Size: 638 192 172 735 367 385 17 1452 Cuts at: 5930 6016 6028 6374 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 735 638 385 367 346 192 172 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5640 Fragments arranged by size: 5640 683 56 BtsI GCAGTG_nn' Cuts at: 2082 2124 2899 3895 4689 4709 2082 Size: 42 775 996 794 20 3752 Fragments arranged by size: 3752 996 794 775 42 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6379 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2893 2928 529 Size: 35 2329 35 3980 Fragments arranged by size: 3980 2329 35 35 DraI TTT'AAA Cuts at: 3363 3683 3363 Size: 320 6059 DraIII CAC_nnn'GTG Cuts at: 3289 3289 Size: 6379 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5386 809 Size: 507 4070 1802 Fragments arranged by size: 4070 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3826 4715 6344 Size: 54 1425 50 275 18 1996 889 1629 Cuts at: 6344 8 Size: 43 Fragments arranged by size: 1996 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3826 1537 Size: 275 2014 4090 Fragments arranged by size: 4090 2014 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2460 2532 2568 2589 4309 1353 Size: 1107 72 36 21 1720 3423 Fragments arranged by size: 3423 1720 1107 72 36 21 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4966 5340 5486 387 Size: 1064 138 3377 374 146 1280 Fragments arranged by size: 3377 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2391 2553 2589 2718 3135 4442 5039 Size: 393 759 162 36 129 417 1307 597 Cuts at: 5039 5826 6094 1239 Size: 787 268 1524 Fragments arranged by size: 1524 1307 787 759 597 417 393 268 162 129 36 EcoNI CCTnn'n_nnAGG Cuts at: 2547 2736 2547 Size: 189 6190 EcoO109I rG'GnC_Cy Cuts at: 2409 2553 2409 Size: 144 6235 EcoRI G'AATT_C Cuts at: 3688 4066 3688 Size: 378 6001 FspI TGC'GCA Cuts at: 4885 4885 Size: 6379 HaeII r_GCGC'y Cuts at: 1678 5526 1678 Size: 3848 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2758 2790 3125 3157 3396 Size: 32 135 32 1186 32 335 32 239 Cuts at: 3396 3428 3639 3671 5020 5052 5094 5126 1373 Size: 32 211 32 1349 32 42 32 2626 Fragments arranged by size: 2626 1349 1186 335 239 211 135 42 32 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2011 2967 3723 3969 669 Size: 988 281 73 956 756 246 3079 Fragments arranged by size: 3079 988 956 756 281 246 73 HindIII A'AGCT_T Cuts at: 748 748 Size: 6379 HpaI GTT'AAC Cuts at: 2967 3969 2967 Size: 1002 5377 KpnI G_GTAC'C Cuts at: 3704 3704 Size: 6379 MfeI C'AATT_G Cuts at: 3978 3978 Size: 6379 MluI A'CGCG_T Cuts at: 6255 6255 Size: 6379 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5493 5677 1305 Size: 4188 184 2007 Fragments arranged by size: 4188 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4557 Fragments arranged by size: 4557 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 2480 4337 4696 4855 518 Size: 648 1173 141 1857 359 159 2042 Fragments arranged by size: 2042 1857 1173 648 359 159 141 MspA1I CmG'CkG Cuts at: 1606 3235 3795 4472 5620 5865 1606 Size: 1629 560 677 1148 245 2120 Fragments arranged by size: 2120 1629 1148 677 560 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4888 Fragments arranged by size: 4888 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6379 NotI GC'GGCC_GC Cuts at: 3826 3826 Size: 6379 NspI r_CATG'y Cuts at: 1132 1229 3737 5282 1132 Size: 97 2508 1545 2229 Fragments arranged by size: 2508 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6379 PciI A'CATG_T Cuts at: 5278 5278 Size: 6379 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6379 PfoI T'CCnGG_A Cuts at: 2613 3582 2613 Size: 969 5410 PmeI GTTT'AAAC Cuts at: 3683 3683 Size: 6379 PmlI CAC'GTG Cuts at: 2912 2912 Size: 6379 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4448 4480 5961 5993 4448 Size: 32 1481 32 4834 Fragments arranged by size: 4834 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2409 2553 2409 Size: 144 6235 PshAI GACnn'nnGTC Cuts at: 1613 2264 1613 Size: 651 5728 PsiI TTA'TAA Cuts at: 2595 3949 2595 Size: 1354 5025 PstI C_TGCA'G Cuts at: 830 3731 830 Size: 2901 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6379 PvuII CAG'CTG Cuts at: 3235 3235 Size: 6379 SalI G'TCGA_C Cuts at: 1936 3721 1936 Size: 1785 4594 SapI GCTCTTCn'nnn_ Cuts at: 2532 2532 Size: 6379 SbfI CC_TGCA'GG Cuts at: 3731 3731 Size: 6379 ScaI AGT'ACT Cuts at: 2356 3426 4627 6339 2356 Size: 1070 1201 1712 2396 Fragments arranged by size: 2396 1712 1201 1070 SfcI C'TryA_G Cuts at: 826 1045 2203 3727 4862 5543 5734 826 Size: 219 1158 1524 1135 681 191 1471 Fragments arranged by size: 1524 1471 1158 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6379 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6379 SmaI CCC'GGG Cuts at: 1872 3706 1872 Size: 1834 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2960 3036 3262 4483 5384 5646 Size: 197 926 1017 76 226 1221 901 262 Cuts at: 5646 820 Size: 1553 Fragments arranged by size: 1553 1221 1017 926 901 262 226 197 76 SnaBI TAC'GTA Cuts at: 493 4170 493 Size: 3677 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6379 SphI G_CATG'C Cuts at: 1132 3737 1132 Size: 2605 3774 SspI AAT'ATT Cuts at: 5 52 2404 3475 3484 3495 3579 4303 5 Size: 47 2352 1071 9 11 84 724 2081 Fragments arranged by size: 2352 2081 1071 724 84 47 11 9 StuI AGG'CCT Cuts at: 1517 2894 1517 Size: 1377 5002 StyI C'CwwG_G Cuts at: 513 1065 2004 3543 3818 513 Size: 552 939 1539 275 3074 Fragments arranged by size: 3074 1539 939 552 275 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4406 4423 4576 4761 5206 325 Size: 1255 2826 17 153 185 445 1498 Fragments arranged by size: 2826 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2354 3424 4625 Size: 274 80 33 51 1443 377 1070 1201 Cuts at: 4625 6337 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1201 1070 377 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6339 6371 6339 Size: 32 6347 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6379 XbaI T'CTAG_A Cuts at: 3715 3715 Size: 6379 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2162 2742 2162 Size: 580 5799 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6379 XmaI C'CCGG_G Cuts at: 1870 3704 1870 Size: 1834 4545 XmnI GAAnn'nnTTC Cuts at: 4508 4508 Size: 6379 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4187 276 Size: 53 83 186 936 2653 2468 Fragments arranged by size: 2653 2468 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SfoI SrfI SwaI