Restriction map for
pFN21AB8772
(Linear) (Six-base) MAPSORT of: KIBB8772.seq Check: 5481 from: 1 to: 1674
KIBB8772.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 584 1674
Size: 386 198 1090
Fragments arranged by size:
1090 386 198
AflII C'TTAA_G
Cuts at: 0 955 1674
Size: 955 719
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 322 1674
Size: 322 1352
AlwNI CAG_nnn'CTG
Cuts at: 0 118 1674
Size: 118 1556
ApoI r'AATT_y
Cuts at: 0 1267 1503 1674
Size: 1267 236 171
Fragments arranged by size:
1267 236 171
AseI AT'TA_AT
Cuts at: 0 1660 1674
Size: 1660 14
BamHI G'GATC_C
Cuts at: 0 969 1674
Size: 969 705
BbsI GAAGACnn'nnnn_
Cuts at: 0 1136 1674
Size: 1136 538
BbvCI CC'TCA_GC
Cuts at: 0 41 119 1674
Size: 41 78 1555
Fragments arranged by size:
1555 78 41
BclI T'GATC_A
Cuts at: 0 230 1406 1674
Size: 230 1176 268
Fragments arranged by size:
1176 268 230
Bme1580I G_kGCm'C
Cuts at: 0 1256 1674
Size: 1256 418
BmrI ACTGGGnnnn_n'
Cuts at: 0 446 1674
Size: 446 1228
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 548 713 1130 1674
Size: 548 165 417 544
Fragments arranged by size:
548 544 417 165
Bpu10I CC'TnA_GC
Cuts at: 0 41 119 1674
Size: 41 78 1555
Fragments arranged by size:
1555 78 41
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1016 1278 1674
Size: 1016 262 396
Fragments arranged by size:
1016 396 262
BsaI GGTCTCn'nnnn_
Cuts at: 0 457 565 748 1674
Size: 457 108 183 926
Fragments arranged by size:
926 457 183 108
BsaAI yAC'GTr
Cuts at: 0 907 1674
Size: 907 767
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 761 1674
Size: 761 913
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 1246 1674
Size: 1246 428
BsiHKAI G_wGCw'C
Cuts at: 0 1625 1674
Size: 1625 49
Bsp1286I G_dGCh'C
Cuts at: 0 1256 1625 1674
Size: 1256 369 49
Fragments arranged by size:
1256 369 49
BspHI T'CATG_A
Cuts at: 0 981 1044 1674
Size: 981 63 630
Fragments arranged by size:
981 630 63
BsrDI GCAATG_nn'
Cuts at: 0 179 1674
Size: 179 1495
BstAPI GCAn_nnn'nTGC
Cuts at: 0 723 1674
Size: 723 951
BstYI r'GATC_y
Cuts at: 0 605 797 969 1674
Size: 605 192 172 705
Fragments arranged by size:
705 605 192 172
BtsI GCAGTG_nn'
Cuts at: 0 77 119 894 1674
Size: 77 42 775 780
Fragments arranged by size:
780 775 77 42
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 888 923 1674
Size: 888 35 751
Fragments arranged by size:
888 751 35
DraI TTT'AAA
Cuts at: 0 1358 1674
Size: 1358 316
DraIII CAC_nnn'GTG
Cuts at: 0 1284 1674
Size: 1284 390
EarI CTCTTCn'nnn_
Cuts at: 0 455 527 563 584 1674
Size: 455 72 36 21 1090
Fragments arranged by size:
1090 455 72 36 21
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 386 548 584 713 1130 1674
Size: 386 162 36 129 417 544
Fragments arranged by size:
544 417 386 162 129 36
EcoNI CCTnn'n_nnAGG
Cuts at: 0 542 731 1674
Size: 542 189 943
Fragments arranged by size:
943 542 189
EcoO109I rG'GnC_Cy
Cuts at: 0 404 548 1674
Size: 404 144 1126
Fragments arranged by size:
1126 404 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 753 785 1120 1152 1391 1423 1634 1666
Size: 753 32 335 32 239 32 211 32
Cuts at: 1666 1674
Size: 8
Fragments arranged by size:
753 335 239 211 32 32 32 32
8
HincII GTy'rAC
Cuts at: 0 6 962 1674
Size: 6 956 712
Fragments arranged by size:
956 712 6
HpaI GTT'AAC
Cuts at: 0 962 1674
Size: 962 712
MslI CAynn'nnrTG
Cuts at: 0 334 475 1674
Size: 334 141 1199
Fragments arranged by size:
1199 334 141
MspA1I CmG'CkG
Cuts at: 0 1230 1674
Size: 1230 444
PfoI T'CCnGG_A
Cuts at: 0 608 1577 1674
Size: 608 969 97
Fragments arranged by size:
969 608 97
PmlI CAC'GTG
Cuts at: 0 907 1674
Size: 907 767
PpuMI rG'GwC_Cy
Cuts at: 0 404 548 1674
Size: 404 144 1126
Fragments arranged by size:
1126 404 144
PshAI GACnn'nnGTC
Cuts at: 0 259 1674
Size: 259 1415
PsiI TTA'TAA
Cuts at: 0 590 1674
Size: 590 1084
PvuII CAG'CTG
Cuts at: 0 1230 1674
Size: 1230 444
SapI GCTCTTCn'nnn_
Cuts at: 0 527 1674
Size: 527 1147
ScaI AGT'ACT
Cuts at: 0 351 1421 1674
Size: 351 1070 253
Fragments arranged by size:
1070 351 253
SfcI C'TryA_G
Cuts at: 0 198 1674
Size: 198 1476
SmlI C'TyrA_G
Cuts at: 0 955 1031 1257 1674
Size: 955 76 226 417
Fragments arranged by size:
955 417 226 76
SspI AAT'ATT
Cuts at: 0 399 1470 1479 1490 1574 1674
Size: 399 1071 9 11 84 100
Fragments arranged by size:
1071 399 100 84 11 9
StuI AGG'CCT
Cuts at: 0 889 1674
Size: 889 785
StyI C'CwwG_G
Cuts at: 0 1538 1674
Size: 1538 136
TatI w'GTAC_w
Cuts at: 0 349 1419 1674
Size: 349 1070 255
Fragments arranged by size:
1070 349 255
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 157 737 1674
Size: 157 580 937
Fragments arranged by size:
937 580 157
Enzymes that do cut:
AcuI AflII AlfI AlwNI ApoI AseI BamHI BbsI
BbvCI BclI Bme1580I BmrI BpmI Bpu10I BpuEI BsaI
BsaAI BseRI BsgI BsiHKAI Bsp1286I BspHI BsrDI BstAPI
BstYI BtsI CspCI DraI DraIII EarI Eco57MI EcoNI
EcoO109I Hin4I HincII HpaI MslI MspA1I PfoI PmlI
PpuMI PshAI PsiI PvuII SapI ScaI SfcI SmlI
SspI StuI StyI TatI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflIII AgeI
AhdI AleI AloI ApaI ApaLI AscI AsiSI AvaI
AvrII BaeI BanI BanII BbeI BcgI BciVI BfrBI
BglI BglII BlpI BmgBI BmtI BplI BsaBI BsaHI
BsaWI BsaXI BseYI BsiEI BsiWI BsmI BsmBI BspEI
BspMI BsrBI BsrFI BsrGI BssHII BssSI BstBI BstEII
BstXI BstZ17I Bsu36I BtgI BtgZI ClaI DrdI EaeI
EagI EciI EcoICRI EcoRI EcoRV FalI FseI FspI
FspAI HaeII HindIII KasI KpnI MfeI MluI MmeI
MscI NaeI NarI NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PasI PciI PflMI PmeI
PpiI PspOMI PspXI PsrI PstI PvuI RsrII SacI
SacII SalI SanDI SbfI SexAI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SwaI TaqII
TstI Tth111I XbaI XhoI XmaI XmnI ZraI