(Circular) (Six-base) MAPSORT of: pFN21AB8771.seq Check: 21 from: 1 to: 5407 pFN21AB8771 5407 bp With 182 enzymes: SgfI * February 27, 2009 14:43 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2216 2218 2344 2216 Size: 2 126 5279 Fragments arranged by size: 5279 126 2 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3217 278 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2750 1937 Size: 813 4594 Acc65I G'GTAC_C Cuts at: 2728 2728 Size: 5407 AclI AA'CG_TT Cuts at: 1563 3534 3907 1563 Size: 1971 373 3063 Fragments arranged by size: 3063 1971 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3470 4854 5122 1632 Size: 1838 1384 268 1917 Fragments arranged by size: 1917 1838 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5210 AflIII A'CryG_T Cuts at: 1203 4306 5283 1203 Size: 3103 977 1327 Fragments arranged by size: 3103 1327 977 AgeI A'CCGG_T Cuts at: 4238 4238 Size: 5407 AhdI GACnn_n'nnGTC Cuts at: 4136 4136 Size: 5407 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5407 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5407 AlwNI CAG_nnn'CTG Cuts at: 3150 4722 3150 Size: 1572 3835 ApaI G_GGCC'C Cuts at: 2632 2632 Size: 5407 ApaLI G'TGCA_C Cuts at: 3464 4620 3464 Size: 1156 4251 ApoI r'AATT_y Cuts at: 1493 2716 2942 3094 5203 1493 Size: 1223 226 152 2109 1697 Fragments arranged by size: 2109 1697 1223 226 152 AscI GG'CGCG_CC Cuts at: 2393 2546 2393 Size: 153 5254 AseI AT'TA_AT Cuts at: 160 1032 3961 160 Size: 872 2929 1606 Fragments arranged by size: 2929 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 AvaI C'yCGr_G Cuts at: 1870 1943 2180 2242 2378 2663 2732 1870 Size: 73 237 62 136 285 69 4545 Fragments arranged by size: 4545 285 237 136 73 69 62 BamHI G'GATC_C Cuts at: 2737 3104 2737 Size: 367 5040 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2098 2131 2167 2175 2277 Size: 325 217 627 311 33 36 8 102 Cuts at: 2277 2318 2615 2728 4183 618 Size: 41 297 113 1455 1842 Fragments arranged by size: 1842 1455 627 325 311 297 217 113 102 41 36 33 8 BanII G_rGCy'C Cuts at: 1391 2330 2632 2726 1391 Size: 939 302 94 4072 Fragments arranged by size: 4072 939 302 94 BbeI G_GCGC'C Cuts at: 2102 2135 2171 2179 2322 2619 2102 Size: 33 36 8 143 297 4890 Fragments arranged by size: 4890 297 143 36 33 8 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4436 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3598 3632 1903 Size: 34 1661 34 3678 Fragments arranged by size: 3678 1661 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3299 4509 3299 Size: 1210 4197 BclI T'GATC_A Cuts at: 1548 1548 Size: 5407 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2613 4018 136 Size: 107 122 71 631 605 941 1405 1525 Fragments arranged by size: 1525 1405 941 631 605 122 107 71 BglII A'GATC_T Cuts at: 5402 5402 Size: 5407 BlpI GC'TnA_GC Cuts at: 2824 2824 Size: 5407 Bme1580I G_kGCm'C Cuts at: 1790 2632 3468 4624 1790 Size: 842 836 1156 2573 Fragments arranged by size: 2573 1156 842 836 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5407 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2099 2209 2398 Size: 416 53 83 399 24 682 110 189 Cuts at: 2398 3138 4096 442 Size: 740 958 1753 Fragments arranged by size: 1753 958 740 682 416 399 189 110 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5407 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2327 2474 2516 4067 1239 Size: 1088 147 42 1551 2579 Fragments arranged by size: 2579 1551 1088 147 42 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2636 3532 4397 4695 2636 Size: 896 865 298 3348 Fragments arranged by size: 3348 896 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4070 882 Size: 617 2571 2219 Fragments arranged by size: 2571 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3198 493 Size: 711 1994 2702 Fragments arranged by size: 2702 1994 711 BsaBI GATnn'nnATC Cuts at: 5401 5401 Size: 5407 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2099 2105 2111 2132 Size: 53 83 186 936 566 6 6 21 Cuts at: 2132 2168 2176 2319 2616 3214 3596 4231 275 Size: 36 8 143 297 598 382 635 1451 Fragments arranged by size: 1451 936 635 598 566 382 297 186 143 83 53 36 21 8 6 6 BsaWI w'CCGG_w Cuts at: 3840 4238 4512 4659 5070 3840 Size: 398 274 147 411 4177 Fragments arranged by size: 4177 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5182 Fragments arranged by size: 5182 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2023 2299 1186 Size: 837 276 4294 Fragments arranged by size: 4294 837 276 BseYI C'CCAG_C Cuts at: 2625 4610 5106 2625 Size: 1985 496 2926 Fragments arranged by size: 2926 1985 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2011 1163 Size: 573 275 4559 Fragments arranged by size: 4559 573 275 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2122 2857 3618 4646 1540 Size: 275 186 121 735 761 1028 2301 Fragments arranged by size: 2301 1028 761 735 275 186 121 BsiHKAI G_wGCw'C Cuts at: 2330 3468 3553 4624 2330 Size: 1138 85 1071 3113 Fragments arranged by size: 3113 1138 1071 85 BsmI GAATG_Cn' Cuts at: 2918 3011 2918 Size: 93 5314 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2330 2632 2726 3468 3553 4624 1391 Size: 399 540 302 94 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 540 399 302 94 85 BspEI T'CCGG_A Cuts at: 5070 5070 Size: 5407 BspHI T'CATG_A Cuts at: 3296 5026 3296 Size: 1730 3677 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2216 2218 2344 2762 844 Size: 1372 2 126 418 3489 Fragments arranged by size: 3489 1372 418 126 2 BsrBI CCG'CTC Cuts at: 1394 1823 2446 2458 2500 2569 2650 3294 1394 Size: 429 623 12 42 69 81 644 3507 Fragments arranged by size: 3507 644 623 429 81 69 42 12 BsrDI GCAATG_nn' Cuts at: 65 1248 3902 4076 65 Size: 1183 2654 174 1396 Fragments arranged by size: 2654 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2020 2095 2295 2436 4051 4238 1700 Size: 110 210 75 200 141 1615 187 2869 Fragments arranged by size: 2869 1615 210 200 187 141 110 75 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5407 BssHII G'CGCG_C Cuts at: 1925 2289 2393 2415 2488 2530 2546 1925 Size: 364 104 22 73 42 16 4786 Fragments arranged by size: 4786 364 104 73 42 22 16 BssSI C'ACGA_G Cuts at: 3467 4479 3467 Size: 1012 4395 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5407 BstBI TT'CG_AA Cuts at: 4211 4211 Size: 5407 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5407 BstYI r'GATC_y Cuts at: 1972 2737 3104 3489 3506 4958 5044 5056 5402 Size: 765 367 385 17 1452 86 12 346 Cuts at: 5402 1972 Size: 1977 Fragments arranged by size: 1977 1452 765 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2282 2282 Size: 5407 BtgI C'CryG_G Cuts at: 513 1065 2031 2044 2236 2472 2535 513 Size: 552 966 13 192 236 63 3385 Fragments arranged by size: 3385 966 552 236 192 63 13 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4668 Fragments arranged by size: 4668 683 56 BtsI GCAGTG_nn' Cuts at: 2923 3717 3737 2923 Size: 794 20 4593 Fragments arranged by size: 4593 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5407 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5372 DraI TTT'AAA Cuts at: 2711 2711 Size: 5407 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2684 4414 809 Size: 507 1368 1730 1802 Fragments arranged by size: 1802 1730 1368 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2119 2421 2469 Size: 54 1425 50 275 18 289 302 48 Cuts at: 2469 2854 3743 5372 8 Size: 385 889 1629 43 Fragments arranged by size: 1629 1425 889 385 302 289 275 54 50 48 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2119 2854 1537 Size: 275 307 735 4090 Fragments arranged by size: 4090 735 307 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2520 3337 1353 Size: 1167 817 3423 Fragments arranged by size: 3423 1167 817 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2521 3994 4368 4514 387 Size: 1064 138 932 1473 374 146 1280 Fragments arranged by size: 1473 1280 1064 932 374 146 138 EcoICRI GAG'CTC Cuts at: 2328 2328 Size: 5407 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2327 2474 2516 3470 4067 4854 5122 Size: 393 695 147 42 954 597 787 268 Cuts at: 5122 1239 Size: 1524 Fragments arranged by size: 1524 954 787 695 597 393 268 147 42 EcoO109I rG'GnC_Cy Cuts at: 2259 2629 2698 2259 Size: 370 69 4968 Fragments arranged by size: 4968 370 69 EcoRI G'AATT_C Cuts at: 2716 3094 2716 Size: 378 5029 FspI TGC'GCA Cuts at: 3913 3913 Size: 5407 HaeII r_GCGC'y Cuts at: 1678 2102 2135 2171 2179 2322 2516 2579 2619 Size: 424 33 36 8 143 194 63 40 Cuts at: 2619 4554 1678 Size: 1935 2531 Fragments arranged by size: 2531 1935 424 194 143 63 40 36 33 8 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4048 4080 4122 4154 1373 Size: 32 135 32 2476 32 42 32 2626 Fragments arranged by size: 2626 2476 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2751 2997 669 Size: 988 281 813 246 3079 Fragments arranged by size: 3079 988 813 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5407 HpaI GTT'AAC Cuts at: 2997 2997 Size: 5407 KasI G'GCGC_C Cuts at: 2098 2131 2167 2175 2318 2615 2098 Size: 33 36 8 143 297 4890 Fragments arranged by size: 4890 297 143 36 33 8 KpnI G_GTAC'C Cuts at: 2732 2732 Size: 5407 MfeI C'AATT_G Cuts at: 3006 3006 Size: 5407 MluI A'CGCG_T Cuts at: 5283 5283 Size: 5407 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4521 4705 1305 Size: 3216 184 2007 Fragments arranged by size: 3216 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3585 Fragments arranged by size: 3585 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3365 3724 3883 518 Size: 648 2199 359 159 2042 Fragments arranged by size: 2199 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2046 2238 2316 2369 2537 2636 2823 3500 Size: 440 192 78 53 168 99 187 677 Cuts at: 3500 4648 4893 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 440 245 192 187 168 99 78 53 NaeI GCC'GGC Cuts at: 2022 2097 2438 2022 Size: 75 341 4991 Fragments arranged by size: 4991 341 75 NarI GG'CG_CC Cuts at: 2099 2132 2168 2176 2319 2616 2099 Size: 33 36 8 143 297 4890 Fragments arranged by size: 4890 297 143 36 33 8 NcoI C'CATG_G Cuts at: 513 1065 2031 513 Size: 552 966 3889 Fragments arranged by size: 3889 966 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5407 NgoMIV G'CCGG_C Cuts at: 2020 2095 2436 2020 Size: 75 341 4991 Fragments arranged by size: 4991 341 75 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5407 NotI GC'GGCC_GC Cuts at: 2854 2854 Size: 5407 NspI r_CATG'y Cuts at: 1132 1229 2765 4310 1132 Size: 97 1536 1545 2229 Fragments arranged by size: 2229 1545 1536 97 PasI CC'CwG_GG Cuts at: 1348 2620 1348 Size: 1272 4135 PciI A'CATG_T Cuts at: 4306 4306 Size: 5407 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5407 PfoI T'CCnGG_A Cuts at: 2083 2083 Size: 5407 PmeI GTTT'AAAC Cuts at: 2711 2711 Size: 5407 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3476 3508 4989 5021 3476 Size: 32 1481 32 3862 Fragments arranged by size: 3862 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2259 2698 2259 Size: 439 4968 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5407 PsiI TTA'TAA Cuts at: 2977 2977 Size: 5407 PspOMI G'GGCC_C Cuts at: 2628 2628 Size: 5407 PstI C_TGCA'G Cuts at: 830 2520 2562 2583 2759 830 Size: 1690 42 21 176 3478 Fragments arranged by size: 3478 1690 176 42 21 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5407 SacI G_AGCT'C Cuts at: 2330 2330 Size: 5407 SacII CC_GC'GG Cuts at: 2047 2239 2538 2047 Size: 192 299 4916 Fragments arranged by size: 4916 299 192 SalI G'TCGA_C Cuts at: 1936 2749 1936 Size: 813 4594 SapI GCTCTTCn'nnn_ Cuts at: 2520 2520 Size: 5407 SbfI CC_TGCA'GG Cuts at: 2759 2759 Size: 5407 ScaI AGT'ACT Cuts at: 3655 5367 3655 Size: 1712 3695 SfcI C'TryA_G Cuts at: 826 1045 2516 2558 2579 2755 3890 4571 4762 Size: 219 1471 42 21 176 1135 681 191 Cuts at: 4762 826 Size: 1471 Fragments arranged by size: 1471 1471 1135 681 219 191 176 42 21 SfoI GGC'GCC Cuts at: 2100 2133 2169 2177 2320 2617 2100 Size: 33 36 8 143 297 4890 Fragments arranged by size: 4890 297 143 36 33 8 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5407 SgrAI Cr'CCGG_yG Cuts at: 1700 2295 1700 Size: 595 4812 SmaI CCC'GGG Cuts at: 1872 2182 2244 2734 1872 Size: 310 62 490 4545 Fragments arranged by size: 4545 490 310 62 SmlI C'TyrA_G Cuts at: 820 1017 1943 2651 3511 4412 4674 820 Size: 197 926 708 860 901 262 1553 Fragments arranged by size: 1553 926 901 860 708 262 197 SnaBI TAC'GTA Cuts at: 493 3198 493 Size: 2705 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5407 SphI G_CATG'C Cuts at: 1132 2765 1132 Size: 1633 3774 SrfI GCCC'GGGC Cuts at: 2244 2244 Size: 5407 SspI AAT'ATT Cuts at: 5 52 3331 5 Size: 47 3279 2081 Fragments arranged by size: 3279 2081 47 StuI AGG'CCT Cuts at: 1517 2147 1517 Size: 630 4777 StyI C'CwwG_G Cuts at: 513 1065 2031 2846 513 Size: 552 966 815 3074 Fragments arranged by size: 3074 966 815 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3434 3451 3604 3789 4234 325 Size: 1255 1854 17 153 185 445 1498 Fragments arranged by size: 1854 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3653 5365 96 Size: 274 80 33 51 1443 1676 1712 138 Fragments arranged by size: 1712 1676 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5367 5399 5367 Size: 32 5375 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5407 XbaI T'CTAG_A Cuts at: 2743 2743 Size: 5407 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5407 XmaI C'CCGG_G Cuts at: 1870 2180 2242 2732 1870 Size: 310 62 490 4545 Fragments arranged by size: 4545 490 310 62 XmnI GAAnn'nnTTC Cuts at: 3536 3536 Size: 5407 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3215 276 Size: 53 83 186 936 1681 2468 Fragments arranged by size: 2468 1681 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AscI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I DraIII EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PmlI PspXI PsrI PvuII RsrII SanDI SexAI SfiI SwaI XcmI