Restriction map for
pFN21AB8769
(Circular) (Six-base) MAPSORT of: pFN21AB8769.seq Check: 1612 from: 1 to: 5404
pFN21AB8769 5404 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3214 278
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2747 1937
Size: 810 4594
Acc65I G'GTAC_C
Cuts at: 2725 2725
Size: 5404
AclI AA'CG_TT
Cuts at: 1563 3531 3904 1563
Size: 1968 373 3063
Fragments arranged by size:
3063 1968 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2182 2438 3467 4851 5119 1632
Size: 550 256 1029 1384 268 1917
Fragments arranged by size:
1917 1384 1029 550 268 256
AfeI AGC'GCT
Cuts at: 2675 2675
Size: 5404
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5207
AflIII A'CryG_T
Cuts at: 1203 4303 5280 1203
Size: 3100 977 1327
Fragments arranged by size:
3100 1327 977
AgeI A'CCGG_T
Cuts at: 4235 4235
Size: 5404
AhdI GACnn_n'nnGTC
Cuts at: 4133 4133
Size: 5404
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5404
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5404
AlwNI CAG_nnn'CTG
Cuts at: 2254 3147 4719 2254
Size: 893 1572 2939
Fragments arranged by size:
2939 1572 893
ApaI G_GGCC'C
Cuts at: 2652 2652
Size: 5404
ApaLI G'TGCA_C
Cuts at: 3461 4617 3461
Size: 1156 4248
ApoI r'AATT_y
Cuts at: 1493 2172 2713 2939 3091 5200 1493
Size: 679 541 226 152 2109 1697
Fragments arranged by size:
2109 1697 679 541 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3958 160
Size: 872 2926 1606
Fragments arranged by size:
2926 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
AvaI C'yCGr_G
Cuts at: 1870 1943 2691 2729 1870
Size: 73 748 38 4545
Fragments arranged by size:
4545 748 73 38
BamHI G'GATC_C
Cuts at: 2734 3101 2734
Size: 367 5037
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2725 4180 618
Size: 325 217 627 938 1455 1842
Fragments arranged by size:
1842 1455 938 627 325 217
BanII G_rGCy'C
Cuts at: 1391 2512 2652 2723 1391
Size: 1121 140 71 4072
Fragments arranged by size:
4072 1121 140 71
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4433
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3595 3629 1903
Size: 34 1658 34 3678
Fragments arranged by size:
3678 1658 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3296 4506 3296
Size: 1210 4194
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5404
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 2023 4015 136
Size: 107 122 71 631 605 351 1992 1525
Fragments arranged by size:
1992 1525 631 605 351 122 107 71
BglII A'GATC_T
Cuts at: 5399 5399
Size: 5404
BlpI GC'TnA_GC
Cuts at: 2821 2821
Size: 5404
Bme1580I G_kGCm'C
Cuts at: 1790 2652 3465 4621 1790
Size: 862 813 1156 2573
Fragments arranged by size:
2573 1156 862 813
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5404
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2100 3135 4093
Size: 416 53 83 399 24 683 1035 958
Cuts at: 4093 442
Size: 1753
Fragments arranged by size:
1753 1035 958 683 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5404
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 4064 1239
Size: 2825 2579
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2537 3529 4394 4692 2537
Size: 992 865 298 3249
Fragments arranged by size:
3249 992 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2269 2433 4067 882
Size: 617 770 164 1634 2219
Fragments arranged by size:
2219 1634 770 617 164
BsaAI yAC'GTr
Cuts at: 493 1204 3195 493
Size: 711 1991 2702
Fragments arranged by size:
2702 1991 711
BsaBI GATnn'nnATC
Cuts at: 5398 5398
Size: 5404
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3211 3593 4228 275
Size: 53 83 186 936 1678 382 635 1451
Fragments arranged by size:
1678 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2082 2442 3837 4235 4509 4656 5067 2082
Size: 360 1395 398 274 147 411 2419
Fragments arranged by size:
2419 1395 411 398 360 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5179
Fragments arranged by size:
5179 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2285 2306 1186
Size: 1099 21 4284
Fragments arranged by size:
4284 1099 21
BseYI C'CCAG_C
Cuts at: 4607 5103 4607
Size: 496 4908
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 4831
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2854 3615 4643 1540
Size: 275 186 853 761 1028 2301
Fragments arranged by size:
2301 1028 853 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3465 3550 4621 3465
Size: 85 1071 4248
Fragments arranged by size:
4248 1071 85
BsmI GAATG_Cn'
Cuts at: 2575 2915 3008 2575
Size: 340 93 4971
Fragments arranged by size:
4971 340 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2512 2652 2723 3465 3550 4621 1391
Size: 399 722 140 71 742 85 1071 2174
Fragments arranged by size:
2174 1071 742 722 399 140 85 71
BspEI T'CCGG_A
Cuts at: 5067 5067
Size: 5404
BspHI T'CATG_A
Cuts at: 3293 5023 3293
Size: 1730 3674
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2759 844
Size: 1915 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 3291 1394
Size: 429 1468 3507
Fragments arranged by size:
3507 1468 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2619 3899 4073 65
Size: 1183 1371 1280 174 1396
Fragments arranged by size:
1396 1371 1280 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4048 4235 1700
Size: 110 2238 187 2869
Fragments arranged by size:
2869 2238 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5404
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5404
BssSI C'ACGA_G
Cuts at: 3464 4476 3464
Size: 1012 4392
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2137 2677 794
Size: 1343 540 3521
Fragments arranged by size:
3521 1343 540
BstBI TT'CG_AA
Cuts at: 4208 4208
Size: 5404
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5404
BstYI r'GATC_y
Cuts at: 1972 2229 2250 2734 3101 3486 3503 4955 5041
Size: 257 21 484 367 385 17 1452 86
Cuts at: 5041 5053 5399 1972
Size: 12 346 1977
Fragments arranged by size:
1977 1452 484 385 367 346 257 86
21 17 12
BtgI C'CryG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 4665
Fragments arranged by size:
4665 683 56
BtsI GCAGTG_nn'
Cuts at: 2920 3714 3734 2920
Size: 794 20 4590
Fragments arranged by size:
4590 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5404
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5369
DraI TTT'AAA
Cuts at: 2708 2708
Size: 5404
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4411 809
Size: 507 3095 1802
Fragments arranged by size:
3095 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2007 2614 2851
Size: 54 1425 50 275 18 177 607 237
Cuts at: 2851 3740 5369 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 889 607 275 237 177 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2851 1537
Size: 275 1039 4090
Fragments arranged by size:
4090 1039 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2157 2199 2262 2283 2349 2559 2580 3334
Size: 804 42 63 21 66 210 21 754
Cuts at: 3334 1353
Size: 3423
Fragments arranged by size:
3423 804 754 210 66 63 42 21
21
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3991 4365 4511 387
Size: 1064 138 2402 374 146 1280
Fragments arranged by size:
2402 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2182 2438 3467 4064 4851 5119 1239
Size: 393 550 256 1029 597 787 268 1524
Fragments arranged by size:
1524 1029 787 597 550 393 268 256
EcoNI CCTnn'n_nnAGG
Cuts at: 2453 2474 2516 2558 2579 2453
Size: 21 42 42 21 5278
Fragments arranged by size:
5278 42 42 21 21
EcoO109I rG'GnC_Cy
Cuts at: 2467 2488 2592 2649 2467
Size: 21 104 57 5222
Fragments arranged by size:
5222 104 57 21
EcoRI G'AATT_C
Cuts at: 2713 3091 2713
Size: 378 5026
FspI TGC'GCA
Cuts at: 3910 3910
Size: 5404
HaeII r_GCGC'y
Cuts at: 1678 2677 4551 1678
Size: 999 1874 2531
Fragments arranged by size:
2531 1874 999
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2214 2246 2303 2335 2534
Size: 32 135 32 642 32 57 32 199
Cuts at: 2534 2566 4045 4077 4119 4151 1373
Size: 32 1479 32 42 32 2626
Fragments arranged by size:
2626 1479 642 199 135 57 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2748 2994 669
Size: 988 281 810 246 3079
Fragments arranged by size:
3079 988 810 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5404
HpaI GTT'AAC
Cuts at: 2994 2994
Size: 5404
KpnI G_GTAC'C
Cuts at: 2729 2729
Size: 5404
MfeI C'AATT_G
Cuts at: 3003 3003
Size: 5404
MluI A'CGCG_T
Cuts at: 5280 5280
Size: 5404
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4518 4702 1305
Size: 3213 184 2007
Fragments arranged by size:
3213 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 2009 2616 10
Size: 54 1425 343 177 607 2798
Fragments arranged by size:
2798 1425 607 343 177 54
MslI CAynn'nnrTG
Cuts at: 518 1166 3362 3721 3880 518
Size: 648 2196 359 159 2042
Fragments arranged by size:
2196 2042 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2820 3497 4645 4890 1606
Size: 1214 677 1148 245 2120
Fragments arranged by size:
2120 1214 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3913
Fragments arranged by size:
3913 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5404
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5404
NotI GC'GGCC_GC
Cuts at: 2851 2851
Size: 5404
NruI TCG'CGA
Cuts at: 2160 2160
Size: 5404
NspI r_CATG'y
Cuts at: 1132 1229 2762 4307 1132
Size: 97 1533 1545 2229
Fragments arranged by size:
2229 1545 1533 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5404
PciI A'CATG_T
Cuts at: 4303 4303
Size: 5404
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2254 1108
Size: 1146 4258
PmeI GTTT'AAAC
Cuts at: 2708 2708
Size: 5404
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3473 3505 4986 5018 3473
Size: 32 1481 32 3859
Fragments arranged by size:
3859 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5404
PsiI TTA'TAA
Cuts at: 2974 2974
Size: 5404
PspOMI G'GGCC_C
Cuts at: 2648 2648
Size: 5404
PstI C_TGCA'G
Cuts at: 830 2142 2756 830
Size: 1312 614 3478
Fragments arranged by size:
3478 1312 614
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5404
SalI G'TCGA_C
Cuts at: 1936 2746 1936
Size: 810 4594
SbfI CC_TGCA'GG
Cuts at: 2756 2756
Size: 5404
ScaI AGT'ACT
Cuts at: 3652 5364 3652
Size: 1712 3692
SfcI C'TryA_G
Cuts at: 826 1045 2138 2752 3887 4568 4759 826
Size: 219 1093 614 1135 681 191 1471
Fragments arranged by size:
1471 1135 1093 681 614 219 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5404
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5404
SmaI CCC'GGG
Cuts at: 1872 2731 1872
Size: 859 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2516 3508 4409 4671 820
Size: 197 926 573 992 901 262 1553
Fragments arranged by size:
1553 992 926 901 573 262 197
SnaBI TAC'GTA
Cuts at: 493 3195 493
Size: 2702 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5404
SphI G_CATG'C
Cuts at: 1132 2762 1132
Size: 1630 3774
SspI AAT'ATT
Cuts at: 5 52 3328 5
Size: 47 3276 2081
Fragments arranged by size:
3276 2081 47
StuI AGG'CCT
Cuts at: 1517 2405 1517
Size: 888 4516
StyI C'CwwG_G
Cuts at: 513 1065 2004 2843 513
Size: 552 939 839 3074
Fragments arranged by size:
3074 939 839 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 3431 3448 3601 3786 4231 325
Size: 1255 1851 17 153 185 445 1498
Fragments arranged by size:
1851 1498 1255 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3650 5362 96
Size: 274 80 33 51 1443 1673 1712 138
Fragments arranged by size:
1712 1673 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 2195 2227 5364 5396 2195
Size: 32 3137 32 2203
Fragments arranged by size:
3137 2203 32 32
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5404
XbaI T'CTAG_A
Cuts at: 2740 2740
Size: 5404
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5404
XmaI C'CCGG_G
Cuts at: 1870 2729 1870
Size: 859 4545
XmnI GAAnn'nnTTC
Cuts at: 2300 2363 3533 2300
Size: 63 1170 4171
Fragments arranged by size:
4171 1170 63
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3212 276
Size: 53 83 186 936 1678 2468
Fragments arranged by size:
2468 1678 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI
AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI
BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI
BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI
BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I
BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII
BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI
ClaI CspCI DraI DrdI EaeI EagI EarI EciI
Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII
HindIII HpaI KpnI MfeI MluI MmeI MscI MslI
MspA1I NcoI NdeI NheI NotI NruI NspI PasI
PciI PflMI PmeI PpiI PshAI PsiI PspOMI PstI
PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI
SmlI SnaBI SpeI SphI SspI StuI StyI TaqII
TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI
BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII
EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI
NgoMIV NsiI PacI PfoI PmlI PpuMI PspXI PsrI
PvuII RsrII SacI SacII SanDI SapI SexAI SfiI
SfoI SrfI SwaI XcmI