Restriction map for
pFN21AB8768
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccIII AclI AcsI
AcyI AflII AflIII AgeI AhdI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp718I AspEI AspHI AspI AvaII
AviII AvrII BalI BamHI BbeI
BbrPI BbsI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BpuAI BsaAI
BsaBI BsaHI BsaI BsaWI BseAI
BseRI BsiEI BsiHKAI BsiWI BsmAI
BsmBI BsmFI BsmI Bsp1407I BspDI
BspEI BspLU11I BsrDI BsrGI BssHII
BssSI Bst1107I BstAPI BstBI BstEII
BstF5I BstPI BstYI BstZ17I Bsu36I
CelII ClaI CpoI DraI DraII
DrdI EaeI EagI Eam1105I EclXI
Eco47III Eco52I Eco81I EcoNI EcoO109I
EcoO65I EcoRI EcoRV EcoT22I FbaI
FokI FseI FspI Hin1I HincII
HindII HindIII HinfI HpaI KasI
KpnI KspI MaeI MamI MfeI
MflI MluI MluNI MroI MscI
MseI MunI NaeI NarI NcoI
NdeI NgoMIV NheI NotI NruI
NsiI NspV PacI PaeR7I PflFI
PinAI PleI PmaCI PmeI PmlI
PpuMI PshAI PshBI Psp1406I PvuI
PvuII RsrII SacI SacII SalI
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI SwaI
TaqI TfiI Tru9I Tth111I TthHB8I
XbaI XhoI XhoII
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# ONE CUT ENZYMES ( 37 enzymes) #
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AccI 343 GTCTAC GT^[AC][GT]AC
Asp700I 381 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BanII 622 GAGCCC G[GA]GC[CT]^C
BmyI 622 GAGCCC G[AGT]GC[ACT]^C
BpmI 616 CTCCAG CTGGAG(16/14)
Bsp1286I 622 GAGCCC G[AGT]GC[ACT]^C
BspHI 189 TCATGA T^CATGA
BsrBI 174 GAGCGG CCGCTC(-3/-3)
BsrFI 610 ACCGGC [GA]^CCGG[CT]
BsrI 225 CCAGT ACTGG(1/-1)
BstXI 437 CCATCGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cfr10I 610 ACCGGC [GA]^CCGG[CT]
DraIII 244 CACGGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 243 CCACGG C^C[GA][CT]GG
EarI 373 GAAGAG CTCTTC(1/4)
EcoT14I 23 CCAAGG C^C[AT][AT]GG
FauI 61 CCCGC CCCGC(4/6)
HaeII 305 AGCGCC [GA]GCGC^[CT]
HgaI 116 GCGTC GACGC(5/10)
Ksp632I 373 GAAGAG CTCTTC(1/4)
MaeII 311 ACGT A^CGT
MslI 678 CATAATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 134 CCGCTG C[AC]G^C[GT]G
NspI 578 ACATGC [GA]CATG^[CT]
PflMI 225 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RcaI 189 TCATGA T^CATGA
SapI 373 GAAGAGC GCTCTTC(1/4)
SfaNI 642 GATGC GCATC(5/9)
SmaI 320 CCCGGG CCC^GGG
StuI 383 AGGCCT AGG^CCT
StyI 23 CCAAGG C^C[AT][AT]GG
Tsp45I 352 GTCAC ^GT[GC]AC
TspRI 654 CACTG CA[GC]TG[ACGT][ACGT]^
Van91I 225 CCAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XmaCI 320 CCCGGG C^CCGGG
XmaI 320 CCCGGG C^CCGGG
XmnI 381 GAAGGCCTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AfaI 152 GTAC GT^AC
195 GTAC
AvaI 320 CCCGGG C^[CT]CG[GA]G
625 CCCGAG
BanI 130 GGCACC G^G[CT][GA]CC
496 GGTGCC
BsoBI 320 CCCGGG C^[CT]CG[GA]G
625 CCCGAG
BspMI 197 ACCTGC ACCTGC(4/8)
422 ACCTGC
Cfr13I 324 GGGCC G^G[ACGT]CC
483 GGCCC
DdeI 348 CTCAG C^T[ACGT]AG
656 CTGAG
HphI 266 TCACC GGTGA(8/7)
353 TCACC
PstI 237 CTGCAG CTGCA^G
301 CTGCAG
RsaI 152 GTAC GT^AC
195 GTAC
Sau96I 324 GGGCC G^G[ACGT]CC
483 GGCCC
SfcI 237 CTGCAG C^T[GA][CT]AG
301 CTGCAG
Tsp509I 520 AATT ^AATT
589 AATT
XcmI 437 CCATCGTGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
500 CCACCTGTGCGATGG
#########################################
# 3 OR MORE CUT ENZYMES ( 39 enzymes) #
#########################################
AccII AciI AluI AlwNI BbvI
BcnI BsaJI BsgI BsiYI BslI
BssKI BstNI BstUI Cac8I CfoI
DpnII EcoRII Fnu4HI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeIII
MboI MboII MnlI MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV Sau3AI ScrFI TseI