Restriction map for
pFN21AB8768
(Circular) (Six-base) MAPSORT of: pFN21AB8768.seq Check: 3259 from: 1 to: 5392
pFN21AB8768 5392 bp
With 182 enzymes: SgfI *
February 27, 2009 14:43 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3202 278
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2349 2735 1937
Size: 412 386 4594
Fragments arranged by size:
4594 412 386
Acc65I G'GTAC_C
Cuts at: 2713 2713
Size: 5392
AclI AA'CG_TT
Cuts at: 1563 3519 3892 1563
Size: 1956 373 3063
Fragments arranged by size:
3063 1956 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2066 2405 3455 4839 5107 1632
Size: 434 339 1050 1384 268 1917
Fragments arranged by size:
1917 1384 1050 434 339 268
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 5195
AflIII A'CryG_T
Cuts at: 1203 4291 5268 1203
Size: 3088 977 1327
Fragments arranged by size:
3088 1327 977
AgeI A'CCGG_T
Cuts at: 4223 4223
Size: 5392
AhdI GACnn_n'nnGTC
Cuts at: 4121 4121
Size: 5392
AleI CACnn'nnGTG
Cuts at: 1166 1166
Size: 5392
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 5392
AlwNI CAG_nnn'CTG
Cuts at: 2041 2236 2575 3135 4707 2041
Size: 195 339 560 1572 2726
Fragments arranged by size:
2726 1572 560 339 195
ApaLI G'TGCA_C
Cuts at: 3449 4605 3449
Size: 1156 4236
ApoI r'AATT_y
Cuts at: 1493 2701 2927 3079 5188 1493
Size: 1208 226 152 2109 1697
Fragments arranged by size:
2109 1697 1208 226 152
AseI AT'TA_AT
Cuts at: 160 1032 3946 160
Size: 872 2914 1606
Fragments arranged by size:
2914 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
AvaI C'yCGr_G
Cuts at: 1870 1943 2325 2630 2717 1870
Size: 73 382 305 87 4545
Fragments arranged by size:
4545 382 305 87 73
BamHI G'GATC_C
Cuts at: 2722 3089 2722
Size: 367 5025
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 2135 2501 2713 4168 618
Size: 325 217 627 348 366 212 1455 1842
Fragments arranged by size:
1842 1455 627 366 348 325 217 212
BanII G_rGCy'C
Cuts at: 1391 2631 2711 1391
Size: 1240 80 4072
Fragments arranged by size:
4072 1240 80
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 4421
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 3583 3617 1903
Size: 34 1646 34 3678
Fragments arranged by size:
3678 1646 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 3284 4494 3284
Size: 1210 4182
BclI T'GATC_A
Cuts at: 1548 1548
Size: 5392
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4003 136
Size: 107 122 71 631 605 2331 1525
Fragments arranged by size:
2331 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 5387 5387
Size: 5392
BlpI GC'TnA_GC
Cuts at: 2809 2809
Size: 5392
Bme1580I G_kGCm'C
Cuts at: 1790 3453 4609 1790
Size: 1663 1156 2573
Fragments arranged by size:
2573 1663 1156
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 5392
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3123 4081 442
Size: 416 53 83 399 24 1706 958 1753
Fragments arranged by size:
1753 1706 958 416 399 83 53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 5392
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2606 4052 1239
Size: 1367 1446 2579
Fragments arranged by size:
2579 1446 1367
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 3517 4382 4680 3517
Size: 865 298 4229
Fragments arranged by size:
4229 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 4055 882
Size: 617 2556 2219
Fragments arranged by size:
2556 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 3183 493
Size: 711 1979 2702
Fragments arranged by size:
2702 1979 711
BsaBI GATnn'nnATC
Cuts at: 5386 5386
Size: 5392
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 3199 3581 4216 275
Size: 53 83 186 936 1666 382 635 1451
Fragments arranged by size:
1666 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 3825 4223 4497 4644 5055 3825
Size: 398 274 147 411 4162
Fragments arranged by size:
4162 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5167
Fragments arranged by size:
5167 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 1186
Size: 5392
BseYI C'CCAG_C
Cuts at: 2236 4595 5091 2236
Size: 2359 496 2537
Fragments arranged by size:
2537 2359 496
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2150 2249 2588 1163
Size: 573 414 99 339 3967
Fragments arranged by size:
3967 573 414 339 99
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2842 3603 4631 1540
Size: 275 186 841 761 1028 2301
Fragments arranged by size:
2301 1028 841 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3453 3538 4609 3453
Size: 85 1071 4236
Fragments arranged by size:
4236 1071 85
BsmI GAATG_Cn'
Cuts at: 2903 2996 2903
Size: 93 5299
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2631 2711 3453 3538 4609 1391
Size: 399 841 80 742 85 1071 2174
Fragments arranged by size:
2174 1071 841 742 399 85 80
BspEI T'CCGG_A
Cuts at: 5055 5055
Size: 5392
BspHI T'CATG_A
Cuts at: 2194 3281 5011 2194
Size: 1087 1730 2575
Fragments arranged by size:
2575 1730 1087
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2211 2436 2747 844
Size: 1367 225 311 3489
Fragments arranged by size:
3489 1367 311 225
BsrBI CCG'CTC
Cuts at: 1394 1823 2181 3279 1394
Size: 429 358 1098 3507
Fragments arranged by size:
3507 1098 429 358
BsrDI GCAATG_nn'
Cuts at: 65 1248 3887 4061 65
Size: 1183 2639 174 1396
Fragments arranged by size:
2639 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 2615 4036 4223 1700
Size: 110 805 1421 187 2869
Fragments arranged by size:
2869 1421 805 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 5392
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 5392
BssSI C'ACGA_G
Cuts at: 3452 4464 3452
Size: 1012 4380
BstAPI GCAn_nnn'nTGC
Cuts at: 794 794
Size: 5392
BstBI TT'CG_AA
Cuts at: 4196 4196
Size: 5392
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 5392
BstXI CCAn_nnnn'nTGG
Cuts at: 2449 2449
Size: 5392
BstYI r'GATC_y
Cuts at: 1972 2722 3089 3474 3491 4943 5029 5041 5387
Size: 750 367 385 17 1452 86 12 346
Cuts at: 5387 1972
Size: 1977
Fragments arranged by size:
1977 1452 750 385 367 346 86 17
12
BtgI C'CryG_G
Cuts at: 513 1065 2004 2248 513
Size: 552 939 244 3657
Fragments arranged by size:
3657 939 552 244
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 2528 487
Size: 683 56 1302 3351
Fragments arranged by size:
3351 1302 683 56
BtsI GCAGTG_nn'
Cuts at: 2908 3702 3722 2908
Size: 794 20 4578
Fragments arranged by size:
4578 794 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 5392
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 5357
DraI TTT'AAA
Cuts at: 2696 2696
Size: 5392
DraIII CAC_nnn'GTG
Cuts at: 2254 2254
Size: 5392
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 4399 809
Size: 507 3083 1802
Fragments arranged by size:
3083 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2839 3728 5357
Size: 54 1425 50 275 18 1009 889 1629
Cuts at: 5357 8
Size: 43
Fragments arranged by size:
1629 1425 1009 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 2839 1537
Size: 275 1027 4090
Fragments arranged by size:
4090 1027 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2373 3322 1353
Size: 1020 949 3423
Fragments arranged by size:
3423 1020 949
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 3979 4353 4499 387
Size: 1064 138 2390 374 146 1280
Fragments arranged by size:
2390 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2066 2405 2606 3455 4052 4839 5107
Size: 393 434 339 201 849 597 787 268
Cuts at: 5107 1239
Size: 1524
Fragments arranged by size:
1524 849 787 597 434 393 339 268
201
EcoRI G'AATT_C
Cuts at: 2701 3079 2701
Size: 378 5014
FspI TGC'GCA
Cuts at: 3898 3898
Size: 5392
HaeII r_GCGC'y
Cuts at: 1678 2314 4539 1678
Size: 636 2225 2531
Fragments arranged by size:
2531 2225 636
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2624 2656 4033 4065 4107
Size: 32 135 32 1052 32 1377 32 42
Cuts at: 4107 4139 1373
Size: 32 2626
Fragments arranged by size:
2626 1377 1052 135 42 32 32 32
32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 2736 2982 669
Size: 988 281 798 246 3079
Fragments arranged by size:
3079 988 798 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 5392
HpaI GTT'AAC
Cuts at: 2982 2982
Size: 5392
KpnI G_GTAC'C
Cuts at: 2717 2717
Size: 5392
MfeI C'AATT_G
Cuts at: 2991 2991
Size: 5392
MluI A'CGCG_T
Cuts at: 5268 5268
Size: 5392
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 4506 4690 1305
Size: 3201 184 2007
Fragments arranged by size:
3201 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 3570
Fragments arranged by size:
3570 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2687 3350 3709 3868 518
Size: 648 1521 663 359 159 2042
Fragments arranged by size:
2042 1521 663 648 359 159
MspA1I CmG'CkG
Cuts at: 1606 2141 2808 3485 4633 4878 1606
Size: 535 667 677 1148 245 2120
Fragments arranged by size:
2120 1148 677 667 535 245
NcoI C'CATG_G
Cuts at: 513 1065 2004 513
Size: 552 939 3901
Fragments arranged by size:
3901 939 552
NdeI CA'TA_TG
Cuts at: 387 387
Size: 5392
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 5392
NotI GC'GGCC_GC
Cuts at: 2839 2839
Size: 5392
NspI r_CATG'y
Cuts at: 1132 1229 2587 2750 4295 1132
Size: 97 1358 163 1545 2229
Fragments arranged by size:
2229 1545 1358 163 97
PasI CC'CwG_GG
Cuts at: 1348 1348
Size: 5392
PciI A'CATG_T
Cuts at: 4291 4291
Size: 5392
PflMI CCAn_nnn'nTGG
Cuts at: 1108 2236 1108
Size: 1128 4264
PfoI T'CCnGG_A
Cuts at: 2622 2622
Size: 5392
PmeI GTTT'AAAC
Cuts at: 2696 2696
Size: 5392
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3461 3493 4974 5006 3461
Size: 32 1481 32 3847
Fragments arranged by size:
3847 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 5392
PsiI TTA'TAA
Cuts at: 2962 2962
Size: 5392
PstI C_TGCA'G
Cuts at: 830 2246 2310 2744 830
Size: 1416 64 434 3478
Fragments arranged by size:
3478 1416 434 64
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 5392
SalI G'TCGA_C
Cuts at: 1936 2734 1936
Size: 798 4594
SapI GCTCTTCn'nnn_
Cuts at: 2373 2373
Size: 5392
SbfI CC_TGCA'GG
Cuts at: 2744 2744
Size: 5392
ScaI AGT'ACT
Cuts at: 3640 5352 3640
Size: 1712 3680
SfcI C'TryA_G
Cuts at: 826 1045 2242 2306 2740 3875 4556 4747 826
Size: 219 1197 64 434 1135 681 191 1471
Fragments arranged by size:
1471 1197 1135 681 434 219 191 64
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 5392
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 5392
SmaI CCC'GGG
Cuts at: 1872 2327 2719 1872
Size: 455 392 4545
Fragments arranged by size:
4545 455 392
SmlI C'TyrA_G
Cuts at: 820 1017 1943 3496 4397 4659 820
Size: 197 926 1553 901 262 1553
Fragments arranged by size:
1553 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 3183 493
Size: 2690 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 5392
SphI G_CATG'C
Cuts at: 1132 2750 1132
Size: 1618 3774
SspI AAT'ATT
Cuts at: 5 52 3316 5
Size: 47 3264 2081
Fragments arranged by size:
3264 2081 47
StuI AGG'CCT
Cuts at: 1517 2390 1517
Size: 873 4519
StyI C'CwwG_G
Cuts at: 513 1065 2004 2028 2831 513
Size: 552 939 24 803 3074
Fragments arranged by size:
3074 939 803 552 24
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2489 2570 3419 3436 3589 3774 4219
Size: 1255 909 81 849 17 153 185 445
Cuts at: 4219 325
Size: 1498
Fragments arranged by size:
1498 1255 909 849 445 185 153 81
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3638 5350 96
Size: 274 80 33 51 1443 1661 1712 138
Fragments arranged by size:
1712 1661 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 5352 5384 5352
Size: 32 5360
Tth111I GACn'n_nGTC
Cuts at: 1316 1316
Size: 5392
XbaI T'CTAG_A
Cuts at: 2728 2728
Size: 5392
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 2449 2512 2449
Size: 63 5329
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 5392
XmaI C'CCGG_G
Cuts at: 1870 2325 2717 1870
Size: 455 392 4545
Fragments arranged by size:
4545 455 392
XmnI GAAnn'nnTTC
Cuts at: 2390 3521 2390
Size: 1131 4261
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3200 276
Size: 53 83 186 936 1666 2468
Fragments arranged by size:
2468 1666 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI
BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI
BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstXI BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DraIII DrdI EaeI EagI EarI EciI
Eco57MI EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NspI PasI PciI PflMI PfoI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XcmI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstZ17I Bsu36I
EcoICRI EcoNI EcoO109I EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI