Restriction map for
pFN21AB8760
##################################
# NO CUT ENZYMES ( 150 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AgeI
AhdI AlwI AocI ApaI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AviII AvrII BalI BamHI
BbeI BbrPI BbvCI BcgI BciVI
BclI BfaI BfrI BglI BlnI
BlpI BpmI Bpu1102I BsaAI BsaBI
BsaHI BsaI BsaWI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrFI BsrGI
BsrI BssHII BssSI Bst1107I BstBI
BstZ17I Bsu36I CelII Cfr10I ClaI
CpoI DraI DraIII EaeI EagI
Eam1105I EarI EclXI Eco52I Eco81I
EcoRI EcoT22I FbaI FseI FspI
Hin1I HincII HindII HindIII HpaI
KasI KpnI Ksp632I MaeI MaeII
MamI MfeI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspV PacI PaeR7I
PflMI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsaI RsrII SacI SalI
SapI SbfI ScaI SfiI SfoI
SfuI SgrAI SmlI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tru9I TthHB8I
Van91I XbaI XcmI XhoI XmnI
###################################
# ONE CUT ENZYMES ( 58 enzymes) #
###################################
AccII 163 CGCG CG^CG
AflIII 566 ACATGT A^C[GA][CT]GT
Alw44I 507 GTGCAC G^TGCAC
AlwNI 418 CAGCCTCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 584 AGCGCT AGC^GCT
ApaLI 507 GTGCAC G^TGCAC
AspHI 507 GTGCAC G[AT]GC[AT]^C
AspI 6 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 367 CCCGGG C^[CT]CG[GA]G
BanII 434 GGGCTC G[GA]GC[CT]^C
BglII 38 AGATCT A^GATCT
BsiHKAI 507 GTGCAC G[AT]GC[AT]^C
BsmBI 4 GAGACG CGTCTC(1/5)
BsmFI 12 GTCCC GGGAC(10/14)
BsoBI 367 CCCGGG C^[CT]CG[GA]G
BspLU11I 566 ACATGT A^CATGT
BspMI 97 ACCTGC ACCTGC(4/8)
BsrDI 287 GCAATG GCAATG(2/0)
BstEII 396 GGTGACC G^GT[ACGT]ACC
BstPI 396 GGTGACC G^GT[ACGT]ACC
BstUI 163 CGCG CG^CG
BstXI 74 CCAAGTTGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 38 AGATCT [GA]^GATC[CT]
DdeI 581 CTAAG C^T[ACGT]AG
DpnII 39 GATC ^GATC
DraII 221 AGGTCCT [GA]G^G[ACGT]CC[CT]
DrdI 214 GACCCGGAGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco47III 584 AGCGCT AGC^GCT
EcoNI 421 CCTCTGCAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO109I 221 AGGTCCT [GA]G^G[ACGT]CC[CT]
EcoO65I 396 GGTGACC G^GT[ACGT]ACC
EcoRV 597 GATATC GAT^ATC
EcoT14I 320 CCAAGG C^C[AT][AT]GG
FauI 471 GCGGG CCCGC(4/6)
HaeII 584 AGCGCT [GA]GCGC^[CT]
HaeIII 71 GGCC GG^CC
HinfI 19 GACTC G^A[ACGT]TC
KspI 162 CCGCGG CCGC^GG
MboI 39 GATC ^GATC
MflI 38 AGATCT [GA]^GATC[CT]
MspA1I 162 CCGCGG C[AC]G^C[GT]G
MvnI 163 CGCG CG^CG
NdeII 39 GATC ^GATC
PflFI 6 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 19 GACTC GAGTC(4/5)
PpuMI 221 AGGTCCT [GA]G^G[AT]CC[CT]
PstI 115 CTGCAG CTGCA^G
SacII 162 CCGCGG CCGC^GG
Sau3AI 39 GATC ^GATC
SexAI 493 ACCTGGT A^CC[AT]GGT
SfaNI 551 GCATC GCATC(5/9)
SmaI 367 CCCGGG CCC^GGG
StyI 320 CCAAGG C^C[AT][AT]GG
Tsp509I 49 AATT ^AATT
Tth111I 6 GACGCTGTC GAC[ACGT]^[ACGT][ACGT]GTC
XhoII 38 AGATCT [GA]^GATC[CT]
XmaCI 367 CCCGGG C^CCGGG
XmaI 367 CCCGGG C^CCGGG
###################################
# TWO CUT ENZYMES ( 14 enzymes) #
###################################
AvaII 128 GGACC G^G[AT]CC
222 GGTCC
BanI 149 GGTGCC G^G[CT][GA]CC
158 GGTGCC
BbsI 268 GTCTTC GAAGAC(2/6)
538 GTCTTC
BpuAI 268 GTCTTC GAAGAC(2/6)
538 GTCTTC
BsmAI 4 GAGAC GTCTC(1/5)
344 GAGAC
BstAPI 287 GCAATGGGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
417 GCAGCCTCTGC
BstF5I 402 CATCC GGATG(2/0)
141 GGATG
DsaI 162 CCGCGG C^C[GA][CT]GG
392 CCGTGG
FokI 402 CATCC GGATG(9/13)
141 GGATG
HgaI 6 GACGC GACGC(5/10)
530 GCGTC
MboII 269 TCTTC GAAGA(8/7)
539 TCTTC
MslI 390 CACCGTGGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
516 CATCTTGGTG
NspI 299 ACATGC [GA]CATG^[CT]
566 ACATGT
SfcI 54 CTACAG C^T[GA][CT]AG
115 CTGCAG
#########################################
# 3 OR MORE CUT ENZYMES ( 36 enzymes) #
#########################################
AciI AluI BbvI BcnI BmyI
BsaJI BseRI BsiYI BslI Bsp1286I
BssKI BstNI Cac8I CfoI Cfr13I
EcoRII Fnu4HI HapII HhaI HinP1I
HpaII HphI ItaI MaeIII MnlI
MspI MvaI MwoI NciI NlaIII
NlaIV Sau96I ScrFI TseI Tsp45I
TspRI