Restriction map for
pFN21AB8760
(Linear) (Six-base) MAPSORT of: KIBB8760.seq Check: 9763 from: 1 to: 609
KIBB8760.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 106 609
Size: 106 503
AfeI AGC'GCT
Cuts at: 0 586 609
Size: 586 23
AflIII A'CryG_T
Cuts at: 0 566 609
Size: 566 43
AleI CACnn'nnGTG
Cuts at: 0 394 609
Size: 394 215
AlwNI CAG_nnn'CTG
Cuts at: 0 423 609
Size: 423 186
ApaLI G'TGCA_C
Cuts at: 0 507 609
Size: 507 102
AvaI C'yCGr_G
Cuts at: 0 367 609
Size: 367 242
BanI G'GyrC_C
Cuts at: 0 149 158 609
Size: 149 9 451
Fragments arranged by size:
451 149 9
BanII G_rGCy'C
Cuts at: 0 438 609
Size: 438 171
BbsI GAAGACnn'nnnn_
Cuts at: 0 261 531 609
Size: 261 270 78
Fragments arranged by size:
270 261 78
BglII A'GATC_T
Cuts at: 0 38 609
Size: 38 571
Bme1580I G_kGCm'C
Cuts at: 0 154 511 609
Size: 154 357 98
Fragments arranged by size:
357 154 98
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 25 609
Size: 25 584
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 469 472 535 609
Size: 469 3 63 74
Fragments arranged by size:
469 74 63 3
BseYI C'CCAG_C
Cuts at: 0 283 609
Size: 283 326
BsiHKAI G_wGCw'C
Cuts at: 0 511 609
Size: 511 98
Bsp1286I G_dGCh'C
Cuts at: 0 154 438 511 609
Size: 154 284 73 98
Fragments arranged by size:
284 154 98 73
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 106 609
Size: 106 503
BsrDI GCAATG_nn'
Cuts at: 0 294 609
Size: 294 315
BstAPI GCAn_nnn'nTGC
Cuts at: 0 293 423 609
Size: 293 130 186
Fragments arranged by size:
293 186 130
BstEII G'GTnAC_C
Cuts at: 0 396 609
Size: 396 213
BstXI CCAn_nnnn'nTGG
Cuts at: 0 81 609
Size: 81 528
BstYI r'GATC_y
Cuts at: 0 38 609
Size: 38 571
BtgI C'CryG_G
Cuts at: 0 162 392 609
Size: 162 230 217
Fragments arranged by size:
230 217 162
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 219 609
Size: 219 390
DrdI GACnn_nn'nnGTC
Cuts at: 0 220 609
Size: 220 389
EcoNI CCTnn'n_nnAGG
Cuts at: 0 425 609
Size: 425 184
EcoO109I rG'GnC_Cy
Cuts at: 0 222 609
Size: 222 387
EcoRV GAT'ATC
Cuts at: 0 599 609
Size: 599 10
HaeII r_GCGC'y
Cuts at: 0 588 609
Size: 588 21
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 27 609
Size: 27 582
MslI CAynn'nnrTG
Cuts at: 0 394 520 609
Size: 394 126 89
Fragments arranged by size:
394 126 89
MspA1I CmG'CkG
Cuts at: 0 164 609
Size: 164 445
NspI r_CATG'y
Cuts at: 0 303 570 609
Size: 303 267 39
Fragments arranged by size:
303 267 39
PciI A'CATG_T
Cuts at: 0 566 609
Size: 566 43
PfoI T'CCnGG_A
Cuts at: 0 206 609
Size: 206 403
PpuMI rG'GwC_Cy
Cuts at: 0 222 609
Size: 222 387
PstI C_TGCA'G
Cuts at: 0 119 609
Size: 119 490
SacII CC_GC'GG
Cuts at: 0 165 609
Size: 165 444
SexAI A'CCwGG_T
Cuts at: 0 493 609
Size: 493 116
SfcI C'TryA_G
Cuts at: 0 54 115 609
Size: 54 61 494
Fragments arranged by size:
494 61 54
SmaI CCC'GGG
Cuts at: 0 369 609
Size: 369 240
StyI C'CwwG_G
Cuts at: 0 320 609
Size: 320 289
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 281 609
Size: 281 328
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 133 165 609
Size: 133 32 444
Fragments arranged by size:
444 133 32
Tth111I GACn'n_nGTC
Cuts at: 0 9 609
Size: 9 600
XmaI C'CCGG_G
Cuts at: 0 367 609
Size: 367 242
Enzymes that do cut:
AarI AfeI AflIII AleI AlwNI ApaLI AvaI BanI
BanII BbsI BglII Bme1580I BsaXI BseRI BseYI BsiHKAI
Bsp1286I BspMI BsrDI BstAPI BstEII BstXI BstYI BtgI
BtgZI DrdI EcoNI EcoO109I EcoRV HaeII Hin4I MslI
MspA1I NspI PciI PfoI PpuMI PstI SacII SexAI
SfcI SmaI StyI TaqII TstI Tth111I XmaI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AflII AgeI AhdI
AlfI AloI ApaI ApoI AscI AseI AsiSI AvrII
BaeI BamHI BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BmrI BmtI BplI BpmI Bpu10I
BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsgI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BsrBI BsrFI BsrGI
BssHII BssSI BstBI BstZ17I Bsu36I BtsI ClaI CspCI
DraI DraIII EaeI EagI EarI EciI EcoICRI Eco57MI
EcoRI FalI FseI FspI FspAI HincII HindIII HpaI
KasI KpnI MfeI MluI MmeI MscI NaeI NarI
NcoI NdeI NgoMIV NheI NotI NruI NsiI PacI
PasI PflMI PmeI PmlI PpiI PshAI PsiI PspOMI
PspXI PsrI PvuI PvuII RsrII SacI SalI SanDI
SapI SbfI ScaI SfiI SfoI SgfI SgrAI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TatI
XbaI XcmI XhoI XmnI ZraI