Restriction map for
pFN21AB8756
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# NO CUT ENZYMES ( 191 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AlwNI AocI
Aor51HI ApaI ApaLI AscI AseI
AsnI Asp700I Asp718I AspEI AspI
AvaI AvaII AviII AvrII BalI
BamHI BanI BbeI BbrPI BbsI
BbvCI BcgI BciVI BcnI BfrI
BglI BglII BlnI BlpI BpmI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaI BsaWI BseAI BseRI BsgI
BsiEI BsiWI BsmAI BsmBI BsmI
BsoBI Bsp1407I BspDI BspEI BspHI
BspLU11I BsrBI BsrFI BsrGI BsrI
BssHII BssSI Bst1107I BstAPI BstBI
BstUI BstXI BstYI BstZ17I Bsu36I
Cac8I CelII CfoI Cfr10I Cfr13I
ClaI CpoI DraI DraII DraIII
DrdI DsaI EaeI EagI Eam1105I
EarI EclXI Eco47III Eco52I Eco81I
EcoO109I EcoRI EcoT22I FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HpaI
HpaII KasI KpnI Ksp632I KspI
MaeII MamI MfeI MflI MluI
MluNI MroI MscI MslI MspA1I
MspI MunI MvnI NaeI NarI
NciI NcoI NdeI NgoMIV NheI
NlaIV NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshBI Psp1406I PvuI PvuII RcaI
RsrII SacII SalI SapI Sau96I
SbfI ScaI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TfiI Tth111I Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
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AluI 469 AGCT AG^CT
AspHI 468 GAGCTC G[AT]GC[AT]^C
BanII 468 GAGCTC G[GA]GC[CT]^C
BclI 554 TGATCA T^GATCA
BfaI 356 CTAG C^TAG
BmyI 468 GAGCTC G[AGT]GC[ACT]^C
BsiHKAI 468 GAGCTC G[AT]GC[AT]^C
BsiYI 148 CCTACCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 148 CCTACCAAAGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 379 GTCCC GGGAC(10/14)
Bsp1286I 468 GAGCTC G[AGT]GC[ACT]^C
BspMI 41 ACCTGC ACCTGC(4/8)
BsrDI 454 GCAATG GCAATG(2/0)
BssKI 482 CCTGG ^CC[ACGT]GG
BstEII 180 GGTAACC G^GT[ACGT]ACC
BstNI 482 CCTGG CC^[AT]GG
BstPI 180 GGTAACC G^GT[ACGT]ACC
DpnII 555 GATC ^GATC
EcoNI 148 CCTACCAAAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoO65I 180 GGTAACC G^GT[ACGT]ACC
EcoRII 482 CCTGG ^CC[AT]GG
EcoRV 360 GATATC GAT^ATC
EcoT14I 185 CCTTGG C^C[AT][AT]GG
FauI 91 GCGGG CCCGC(4/6)
FbaI 554 TGATCA T^GATCA
HphI 460 GGTGA GGTGA(8/7)
MaeI 356 CTAG C^TAG
MboI 555 GATC ^GATC
MnlI 414 GAGG CCTC(7/6)
MvaI 482 CCTGG CC^[AT]GG
NdeII 555 GATC ^GATC
NlaIII 547 CATG CATG^
PshAI 99 GACAACTGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
PstI 118 CTGCAG CTGCA^G
SacI 468 GAGCTC GAGCT^C
Sau3AI 555 GATC ^GATC
ScrFI 482 CCTGG CC^[ACGT]GG
SfaNI 272 GATGC GCATC(5/9)
SfcI 118 CTGCAG C^T[GA][CT]AG
SmlI 535 CTTGAG C^T[CT][GA]AG
StyI 185 CCTTGG C^C[AT][AT]GG
TaqI 314 TCGA T^CGA
TthHB8I 314 TCGA T^CGA
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# TWO CUT ENZYMES ( 18 enzymes) #
###################################
AciI 91 GCGG CCGC(-3/-1)
579 GCGG
AcsI 130 GAATTT [GA]^AATT[CT]
192 AAATTC
AfaI 145 GTAC GT^AC
385 GTAC
ApoI 130 GAATTT [GA]^AATT[CT]
192 AAATTC
BbvI 492 GCAGC GCAGC(8/12)
117 GCTGC
BsaJI 185 CCTTGG C^C[ACGT][ACGT]GG
481 CCCTGG
BstF5I 222 GGATG GGATG(2/0)
320 GGATG
DdeI 167 CTGAG C^T[ACGT]AG
466 CTGAG
FokI 222 GGATG GGATG(9/13)
320 GGATG
HaeIII 248 GGCC GG^CC
520 GGCC
HinfI 301 GACTC G^A[ACGT]TC
377 GAGTC
MboII 325 GAAGA GAAGA(8/7)
566 GAAGA
MwoI 275 GCACAGATGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
486 GCATTTGCAGC
PleI 301 GACTC GAGTC(4/5)
377 GAGTC
RsaI 145 GTAC GT^AC
385 GTAC
TseI 117 GCTGC G^C[AT]GC
492 GCAGC
Tsp45I 205 GTCAC ^GT[GC]AC
426 GTCAC
TspRI 165 CACTG CA[GC]TG[ACGT][ACGT]^
251 CACTG
#########################################
# 3 OR MORE CUT ENZYMES ( 6 enzymes) #
#########################################
Fnu4HI ItaI MaeIII MseI Tru9I
Tsp509I