Restriction map for
pFN21AB8755
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# NO CUT ENZYMES ( 168 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI AocI Aor51HI ApaI
ApaLI ApoI AscI AseI AsnI
Asp700I Asp718I AspI AvaI AviII
AvrII BalI BamHI BanI BbeI
BbrPI BcgI BciVI BfaI BfrI
BglI BglII BlnI BlpI Bpu1102I
BsaAI BsaBI BsaHI BsaWI BseAI
BsiEI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BspMI BsrBI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I Bsu36I CelII ClaI
CpoI DraI DraII DraIII DrdI
DsaI EagI EarI EclXI Eco47III
Eco52I Eco81I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FseI FspI
HaeII HgaI Hin1I HincII HindII
HindIII HpaI KasI KpnI Ksp632I
KspI MaeI MamI MfeI MflI
MluI MluNI MroI MscI MseI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmaCI PmeI PmlI PpuMI
PshAI PshBI Psp1406I PstI PvuI
RcaI RsrII SacII SalI SapI
SbfI ScaI SexAI SfcI SfiI
SfoI SfuI SgrAI SmaI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp509I TspRI
Tth111I Van91I XbaI XhoI XhoII
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 39 enzymes) #
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AccII 385 CGCG CG^CG
AfaI 255 GTAC GT^AC
AhdI 516 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AspEI 516 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 515 GGACC G^G[AT]CC
BbsI 121 GAAGAC GAAGAC(2/6)
BbvCI 449 CCTCAGC CCTCAGC(-5/-2)
BpuAI 121 GAAGAC GAAGAC(2/6)
BsaI 445 GAGACC GGTCTC(1/5)
BseRI 283 GAGGAG GAGGAG(10/8)
BsgI 391 GTGCAG GTGCAG(16/14)
BsmFI 326 GGGAC GGGAC(10/14)
BsrDI 167 CATTGC GCAATG(2/0)
BsrFI 131 ACCGGC [GA]^CCGG[CT]
BsrI 198 CCAGT ACTGG(1/-1)
BstUI 385 CGCG CG^CG
BstXI 344 CCATCATGCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
CfoI 510 GCGC GCG^C
Cfr10I 131 ACCGGC [GA]^CCGG[CT]
Eam1105I 516 GACCGTGAGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
FauI 419 CCCGC CCCGC(4/6)
HhaI 510 GCGC GCG^C
HinP1I 510 GCGC G^CGC
HinfI 522 GAGTC G^A[ACGT]TC
HphI 176 TCACC GGTGA(8/7)
MboII 121 GAAGA GAAGA(8/7)
MvnI 385 CGCG CG^CG
NdeI 159 CATATG CA^TATG
NlaIV 191 GGAGCC GG[ACGT]^[ACGT]CC
PleI 522 GAGTC GAGTC(4/5)
PvuII 144 CAGCTG CAG^CTG
RsaI 255 GTAC GT^AC
SfaNI 338 GCATC GCATC(5/9)
SmlI 289 CTCAAG C^T[CT][GA]AG
StuI 410 AGGCCT AGG^CCT
TaqI 533 TCGA T^CGA
Tsp45I 149 GTGAC ^GT[GC]AC
TthHB8I 533 TCGA T^CGA
XcmI 344 CCATCATGCTGGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
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# TWO CUT ENZYMES ( 18 enzymes) #
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AlwNI 214 CAGCGGCTG CAG[ACGT][ACGT][ACGT]^CTG
487 CAGGAGCTG
AspHI 60 GAGCTC G[AT]GC[AT]^C
286 GAGCTC
BclI 140 TGATCA T^GATCA
590 TGATCA
BpmI 526 CTCCAG CTGGAG(16/14)
502 CTGGAG
BsiHKAI 60 GAGCTC G[AT]GC[AT]^C
286 GAGCTC
BsmAI 445 GAGAC GTCTC(1/5)
524 GTCTC
Cfr13I 417 GGCCC G^G[ACGT]CC
515 GGACC
DdeI 5 CTGAG C^T[ACGT]AG
450 CTCAG
EaeI 29 TGGCCG [CT]^GGCC[GA]
206 CGGCCA
EcoT14I 278 CCTTGG C^C[AT][AT]GG
413 CCTTGG
FbaI 140 TGATCA T^GATCA
590 TGATCA
MaeII 329 ACGT A^CGT
470 ACGT
MaeIII 14 GTAAC ^GT[ACGT]AC
149 GTGAC
MspA1I 144 CAGCTG C[AC]G^C[GT]G
214 CAGCGG
MwoI 47 GCGGTGTTGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
377 GCCCCAGCCGC
SacI 60 GAGCTC GAGCT^C
286 GAGCTC
Sau96I 417 GGCCC G^G[ACGT]CC
515 GGACC
StyI 278 CCTTGG C^C[AT][AT]GG
413 CCTTGG
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# 3 OR MORE CUT ENZYMES ( 33 enzymes) #
#########################################
AciI AluI BanII BbvI BcnI
BmyI BsaJI BsiYI BslI Bsp1286I
BssKI BstF5I BstNI Cac8I DpnII
EcoRII Fnu4HI FokI HaeIII HapII
HpaII ItaI MboI MnlI MslI
MspI MvaI NciI NdeII NlaIII
Sau3AI ScrFI TseI