Restriction map for
pFN21AB8755
(Linear) (Six-base) MAPSORT of: KIBB8755.seq Check: 5716 from: 1 to: 597
KIBB8755.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AhdI GACnn_n'nnGTC
Cuts at: 0 521 597
Size: 521 76
AlwNI CAG_nnn'CTG
Cuts at: 0 219 492 597
Size: 219 273 105
Fragments arranged by size:
273 219 105
BanII G_rGCy'C
Cuts at: 0 64 290 379 597
Size: 64 226 89 218
Fragments arranged by size:
226 218 89 64
BbsI GAAGACnn'nnnn_
Cuts at: 0 128 597
Size: 128 469
BbvCI CC'TCA_GC
Cuts at: 0 450 597
Size: 450 147
BclI T'GATC_A
Cuts at: 0 140 590 597
Size: 140 450 7
Fragments arranged by size:
450 140 7
Bme1580I G_kGCm'C
Cuts at: 0 239 597
Size: 239 358
BmgBI CAC'GTC
Cuts at: 0 330 597
Size: 330 267
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 523 597
Size: 511 12 74
Fragments arranged by size:
511 74 12
Bpu10I CC'TnA_GC
Cuts at: 0 5 450 597
Size: 5 445 147
Fragments arranged by size:
445 147 5
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 274 597
Size: 274 323
BsaI GGTCTCn'nnnn_
Cuts at: 0 439 597
Size: 439 158
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 298 597
Size: 298 299
BseYI C'CCAG_C
Cuts at: 0 379 597
Size: 379 218
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 412 597
Size: 412 185
BsiHKAI G_wGCw'C
Cuts at: 0 64 290 597
Size: 64 226 307
Fragments arranged by size:
307 226 64
Bsp1286I G_dGCh'C
Cuts at: 0 64 239 290 379 597
Size: 64 175 51 89 218
Fragments arranged by size:
218 175 89 64 51
BsrDI GCAATG_nn'
Cuts at: 0 166 597
Size: 166 431
BsrFI r'CCGG_y
Cuts at: 0 131 597
Size: 131 466
BstXI CCAn_nnnn'nTGG
Cuts at: 0 351 597
Size: 351 246
EaeI y'GGCC_r
Cuts at: 0 29 206 597
Size: 29 177 391
Fragments arranged by size:
391 177 29
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 421 597
Size: 421 176
EcoICRI GAG'CTC
Cuts at: 0 62 288 597
Size: 62 226 309
Fragments arranged by size:
309 226 62
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 511 523 597
Size: 511 12 74
Fragments arranged by size:
511 74 12
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 453 597
Size: 453 144
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 507 539 597
Size: 507 32 58
Fragments arranged by size:
507 58 32
MslI CAynn'nnrTG
Cuts at: 0 250 352 429 597
Size: 250 102 77 168
Fragments arranged by size:
250 168 102 77
MspA1I CmG'CkG
Cuts at: 0 146 216 597
Size: 146 70 381
Fragments arranged by size:
381 146 70
NdeI CA'TA_TG
Cuts at: 0 160 597
Size: 160 437
PfoI T'CCnGG_A
Cuts at: 0 95 485 597
Size: 95 390 112
Fragments arranged by size:
390 112 95
PvuII CAG'CTG
Cuts at: 0 146 597
Size: 146 451
SacI G_AGCT'C
Cuts at: 0 64 290 597
Size: 64 226 307
Fragments arranged by size:
307 226 64
SmlI C'TyrA_G
Cuts at: 0 289 597
Size: 289 308
StuI AGG'CCT
Cuts at: 0 412 597
Size: 412 185
StyI C'CwwG_G
Cuts at: 0 278 413 597
Size: 278 135 184
Fragments arranged by size:
278 184 135
TatI w'GTAC_w
Cuts at: 0 254 597
Size: 254 343
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 351 383 597
Size: 351 32 214
Fragments arranged by size:
351 214 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 351 597
Size: 351 246
Enzymes that do cut:
AhdI AlwNI BanII BbsI BbvCI BclI Bme1580I BmgBI
BpmI Bpu10I BpuEI BsaI BseRI BseYI BsgI BsiHKAI
Bsp1286I BsrDI BsrFI BstXI EaeI EciI EcoICRI Eco57MI
FalI Hin4I MslI MspA1I NdeI PfoI PvuII SacI
SmlI StuI StyI TatI TstI XcmI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AcuI AfeI AflII
AflIII AgeI AleI AlfI AloI ApaI ApaLI ApoI
AscI AseI AsiSI AvaI AvrII BaeI BamHI BanI
BbeI BcgI BciVI BfrBI BglI BglII BlpI BmrI
BmtI BplI BsaAI BsaBI BsaHI BsaWI BsaXI BsiEI
BsiWI BsmI BsmBI BspEI BspHI BspMI BsrBI BsrGI
BssHII BssSI BstAPI BstBI BstEII BstYI BstZ17I Bsu36I
BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI
EagI EarI EcoNI EcoO109I EcoRI EcoRV FseI FspI
FspAI HaeII HincII HindIII HpaI KasI KpnI MfeI
MluI MmeI MscI NaeI NarI NcoI NgoMIV NheI
NotI NruI NsiI NspI PacI PasI PciI PflMI
PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PspXI
PsrI PstI PvuI RsrII SacII SalI SanDI SapI
SbfI ScaI SexAI SfcI SfiI SfoI SgfI SgrAI
SmaI SnaBI SpeI SphI SrfI SspI SwaI TaqII
Tth111I XbaI XhoI XmaI XmnI ZraI