Restriction map for
pFN21AB8754
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# NO CUT ENZYMES ( 166 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AfaI AflII AflIII
AgeI AhdI AlwI AocI ApoI
AscI AseI AsnI Asp700I Asp718I
AspEI AvaI AvaII AviII AvrII
BalI BamHI BanI BbeI BbrPI
BbsI BbvCI BcgI BciVI BfaI
BfrI BglI BglII BlnI BlpI
Bpu1102I BpuAI BsaAI BsaBI BsaHI
BsaWI BseAI BsiEI BsiWI BsmFI
BsmI BsoBI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrGI
BssHII Bst1107I BstBI BstEII BstPI
BstXI BstYI BstZ17I Bsu36I CelII
ClaI CpoI DdeI DraI DraIII
DrdI DsaI EagI Eam1105I EclXI
Eco52I Eco81I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI Hin1I
HincII HindII HindIII HinfI HpaI
KasI KpnI KspI MaeI MamI
MfeI MflI MluI MluNI MroI
MscI MseI MunI NaeI NarI
NcoI NdeI NgoMIV NheI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflMI PinAI PleI PmaCI
PmeI PmlI PpuMI PshAI PshBI
Psp1406I PvuI RcaI RsaI RsrII
SacII SalI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmaI
SmlI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI TfiI
Tru9I Tsp509I TspRI Van91I XbaI
XcmI XhoI XhoII XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 27 enzymes) #
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Alw44I 162 GTGCAC G^TGCAC
Aor51HI 539 AGCGCT AGC^GCT
ApaI 292 GGGCCC GGGCC^C
ApaLI 162 GTGCAC G^TGCAC
AspI 565 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BclI 140 TGATCA T^GATCA
BsaI 442 GAGACC GGTCTC(1/5)
BseRI 283 GAGGAG GAGGAG(10/8)
BsgI 313 GTGCAG GTGCAG(16/14)
BspMI 135 GCAGGT ACCTGC(4/8)
BsrI 198 CCAGT ACTGG(1/-1)
BssSI 352 CTCGTG CACGAG(-5/-1)
BstAPI 359 GCAACAAGTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
DraII 291 GGGGCCC [GA]G^G[ACGT]CC[CT]
Eco47III 539 AGCGCT AGC^GCT
EcoO109I 291 GGGGCCC [GA]G^G[ACGT]CC[CT]
EcoT14I 230 CCAAGG C^C[AT][AT]GG
FbaI 140 TGATCA T^GATCA
PflFI 565 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PstI 169 CTGCAG CTGCA^G
PvuII 144 CAGCTG CAG^CTG
SapI 477 GCTCTTC GCTCTTC(1/4)
SfaNI 348 GATGC GCATC(5/9)
SfcI 169 CTGCAG C^T[GA][CT]AG
StyI 230 CCAAGG C^C[AT][AT]GG
Tsp45I 262 GTCAC ^GT[GC]AC
Tth111I 565 GACCGCGTC GAC[ACGT]^[ACGT][ACGT]GTC
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# TWO CUT ENZYMES ( 20 enzymes) #
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BcnI 204 CCCGG CC^[GC]GG
545 CCGGG
BpmI 280 CTGGAG CTGGAG(16/14)
499 CTGGAG
BsmBI 373 GAGACG CGTCTC(1/5)
502 GAGACG
BsrFI 131 ACCGGC [GA]^CCGG[CT]
187 ACCGGC
Cfr10I 131 ACCGGC [GA]^CCGG[CT]
187 ACCGGC
EaeI 206 CGGCCA [CT]^GGCC[GA]
449 CGGCCA
EarI 478 CTCTTC CTCTTC(1/4)
555 GAAGAG
FauI 43 GCGGG CCCGC(4/6)
381 GCGGG
HaeII 215 AGCGCC [GA]GCGC^[CT]
539 AGCGCT
HphI 138 GGTGA GGTGA(8/7)
263 TCACC
Ksp632I 478 CTCTTC CTCTTC(1/4)
555 GAAGAG
MaeII 91 ACGT A^CGT
467 ACGT
MaeIII 14 GTAAC ^GT[ACGT]AC
262 GTCAC
MboII 555 GAAGA GAAGA(8/7)
479 TCTTC
MspA1I 144 CAGCTG C[AC]G^C[GT]G
379 CAGCGG
NciI 204 CCCGG CC^[GC]GG
545 CCGGG
NlaIV 291 GGGGCC GG[ACGT]^[ACGT]CC
292 GGGCCC
SacI 60 GAGCTC GAGCT^C
475 GAGCTC
TaqI 119 TCGA T^CGA
530 TCGA
TthHB8I 119 TCGA T^CGA
530 TCGA
#########################################
# 3 OR MORE CUT ENZYMES ( 45 enzymes) #
#########################################
AccII AciI AluI AlwNI AspHI
BanII BbvI BmyI BsaJI BsiHKAI
BsiYI BslI BsmAI Bsp1286I BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DpnII EcoRII Fnu4HI FokI
HaeIII HapII HgaI HhaI HinP1I
HpaII ItaI MboI MnlI MslI
MspI MvaI MvnI MwoI NdeII
NlaIII Sau3AI Sau96I ScrFI TseI