(Circular) (Six-base) MAPSORT of: pFN21AB8754.seq Check: 6105 from: 1 to: 5299 pFN21AB8754 5299 bp With 182 enzymes: SgfI * February 27, 2009 14:42 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2131 2131 Size: 5299 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3109 278 Size: 53 83 186 936 1573 2468 Fragments arranged by size: 2468 1573 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2642 1937 Size: 705 4594 Acc65I G'GTAC_C Cuts at: 2620 2620 Size: 5299 AclI AA'CG_TT Cuts at: 1563 3426 3799 1563 Size: 1863 373 3063 Fragments arranged by size: 3063 1863 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3362 4746 5014 1632 Size: 1730 1384 268 1917 Fragments arranged by size: 1917 1730 1384 268 AfeI AGC'GCT Cuts at: 2546 2546 Size: 5299 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5102 AflIII A'CryG_T Cuts at: 1203 4198 5175 1203 Size: 2995 977 1327 Fragments arranged by size: 2995 1327 977 AgeI A'CCGG_T Cuts at: 4130 4130 Size: 5299 AhdI GACnn_n'nnGTC Cuts at: 4028 4028 Size: 5299 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5299 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5299 AlwNI CAG_nnn'CTG Cuts at: 2224 2284 2494 3042 4614 2224 Size: 60 210 548 1572 2909 Fragments arranged by size: 2909 1572 548 210 60 ApaI G_GGCC'C Cuts at: 2301 2301 Size: 5299 ApaLI G'TGCA_C Cuts at: 2167 3356 4512 2167 Size: 1189 1156 2954 Fragments arranged by size: 2954 1189 1156 ApoI r'AATT_y Cuts at: 1493 2608 2834 2986 5095 1493 Size: 1115 226 152 2109 1697 Fragments arranged by size: 2109 1697 1115 226 152 AseI AT'TA_AT Cuts at: 160 1032 3853 160 Size: 872 2821 1606 Fragments arranged by size: 2821 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 AvaI C'yCGr_G Cuts at: 1870 1943 2624 1870 Size: 73 681 4545 Fragments arranged by size: 4545 681 73 BamHI G'GATC_C Cuts at: 2629 2996 2629 Size: 367 4932 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2620 4075 618 Size: 325 217 627 833 1455 1842 Fragments arranged by size: 1842 1455 833 627 325 217 BanII G_rGCy'C Cuts at: 1391 2069 2301 2484 2618 1391 Size: 678 232 183 134 4072 Fragments arranged by size: 4072 678 232 183 134 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4328 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 3490 3524 1903 Size: 34 50 34 1469 34 3678 Fragments arranged by size: 3678 1469 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3191 4401 3191 Size: 1210 4089 BclI T'GATC_A Cuts at: 1548 2145 1548 Size: 597 4702 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3910 136 Size: 107 122 71 631 605 2238 1525 Fragments arranged by size: 2238 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5294 5294 Size: 5299 BlpI GC'TnA_GC Cuts at: 2716 2716 Size: 5299 Bme1580I G_kGCm'C Cuts at: 1790 2096 2171 2301 3360 4516 1790 Size: 306 75 130 1059 1156 2573 Fragments arranged by size: 2573 1156 1059 306 130 75 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5299 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3030 3988 442 Size: 416 53 83 399 24 1613 958 1753 Fragments arranged by size: 1753 1613 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5299 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2306 2525 3959 1239 Size: 1067 219 1434 2579 Fragments arranged by size: 2579 1434 1067 219 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3424 4289 4587 3424 Size: 865 298 4136 Fragments arranged by size: 4136 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2441 3962 882 Size: 617 942 1521 2219 Fragments arranged by size: 2219 1521 942 617 BsaAI yAC'GTr Cuts at: 493 1204 3090 493 Size: 711 1886 2702 Fragments arranged by size: 2702 1886 711 BsaBI GATnn'nnATC Cuts at: 5293 5293 Size: 5299 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3106 3488 4123 275 Size: 53 83 186 936 1573 382 635 1451 Fragments arranged by size: 1573 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3732 4130 4404 4551 4962 3732 Size: 398 274 147 411 4069 Fragments arranged by size: 4069 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2417 2447 1178 Size: 30 165 30 1014 30 4030 Fragments arranged by size: 4030 1014 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2303 1186 Size: 1117 4182 BseYI C'CCAG_C Cuts at: 2293 4502 4998 2293 Size: 2209 496 2594 Fragments arranged by size: 2594 2209 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2339 1163 Size: 573 603 4123 Fragments arranged by size: 4123 603 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2749 3510 4538 1540 Size: 275 186 748 761 1028 2301 Fragments arranged by size: 2301 1028 761 748 275 186 BsiHKAI G_wGCw'C Cuts at: 2069 2171 2484 3360 3445 4516 2069 Size: 102 313 876 85 1071 2852 Fragments arranged by size: 2852 1071 876 313 102 85 BsmI GAATG_Cn' Cuts at: 2810 2903 2810 Size: 93 5206 BsmBI CGTCTCn'nnnn_ Cuts at: 2372 2501 2372 Size: 129 5170 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2069 2096 2171 2301 2484 2618 3360 Size: 399 279 27 75 130 183 134 742 Cuts at: 3360 3445 4516 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 279 183 134 130 85 75 27 BspEI T'CCGG_A Cuts at: 4962 4962 Size: 5299 BspHI T'CATG_A Cuts at: 3188 4918 3188 Size: 1730 3569 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2131 2654 844 Size: 1287 523 3489 Fragments arranged by size: 3489 1287 523 BsrBI CCG'CTC Cuts at: 1394 1823 3186 1394 Size: 429 1363 3507 Fragments arranged by size: 3507 1363 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3794 3968 65 Size: 1183 2546 174 1396 Fragments arranged by size: 2546 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2136 2192 3943 4130 1700 Size: 110 326 56 1751 187 2869 Fragments arranged by size: 2869 1751 326 187 110 56 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5299 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5299 BssSI C'ACGA_G Cuts at: 2357 3359 4371 2357 Size: 1002 1012 3285 Fragments arranged by size: 3285 1012 1002 BstAPI GCAn_nnn'nTGC Cuts at: 794 2370 794 Size: 1576 3723 BstBI TT'CG_AA Cuts at: 4103 4103 Size: 5299 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5299 BstYI r'GATC_y Cuts at: 1972 2629 2996 3381 3398 4850 4936 4948 5294 Size: 657 367 385 17 1452 86 12 346 Cuts at: 5294 1972 Size: 1977 Fragments arranged by size: 1977 1452 657 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4747 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2388 487 Size: 683 56 1162 3398 Fragments arranged by size: 3398 1162 683 56 BtsI GCAGTG_nn' Cuts at: 2815 3609 3629 2815 Size: 794 20 4485 Fragments arranged by size: 4485 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5299 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5264 DraI TTT'AAA Cuts at: 2603 2603 Size: 5299 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4306 809 Size: 507 2990 1802 Fragments arranged by size: 2990 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2211 2454 2746 Size: 54 1425 50 275 18 381 243 292 Cuts at: 2746 3635 5264 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 381 292 275 243 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2746 1537 Size: 275 934 4090 Fragments arranged by size: 4090 934 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2489 2555 3229 1353 Size: 1136 66 674 3423 Fragments arranged by size: 3423 1136 674 66 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3886 4260 4406 387 Size: 1064 138 2297 374 146 1280 Fragments arranged by size: 2297 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2067 2482 2067 Size: 415 4884 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2306 2525 3362 3959 4746 5014 1239 Size: 393 674 219 837 597 787 268 1524 Fragments arranged by size: 1524 837 787 674 597 393 268 219 EcoO109I rG'GnC_Cy Cuts at: 2297 2297 Size: 5299 EcoRI G'AATT_C Cuts at: 2608 2986 2608 Size: 378 4921 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2500 2500 Size: 5299 FspI TGC'GCA Cuts at: 3805 3805 Size: 5299 HaeII r_GCGC'y Cuts at: 1678 2224 2548 4446 1678 Size: 546 324 1898 2531 Fragments arranged by size: 2531 1898 546 324 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3940 3972 4014 4046 1373 Size: 32 135 32 2368 32 42 32 2626 Fragments arranged by size: 2626 2368 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2643 2889 669 Size: 988 281 705 246 3079 Fragments arranged by size: 3079 988 705 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5299 HpaI GTT'AAC Cuts at: 2889 2889 Size: 5299 KpnI G_GTAC'C Cuts at: 2624 2624 Size: 5299 MfeI C'AATT_G Cuts at: 2898 2898 Size: 5299 MluI A'CGCG_T Cuts at: 5175 5175 Size: 5299 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4413 4597 1305 Size: 3108 184 2007 Fragments arranged by size: 3108 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3477 Fragments arranged by size: 3477 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2255 2348 2594 3257 3616 3775 518 Size: 648 1089 93 246 663 359 159 2042 Fragments arranged by size: 2042 1089 663 648 359 246 159 93 MspA1I CmG'CkG Cuts at: 1606 2151 2386 2715 3392 4540 4785 1606 Size: 545 235 329 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 545 329 245 235 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4747 NdeI CA'TA_TG Cuts at: 387 387 Size: 5299 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5299 NotI GC'GGCC_GC Cuts at: 2746 2746 Size: 5299 NspI r_CATG'y Cuts at: 1132 1229 2657 4202 1132 Size: 97 1428 1545 2229 Fragments arranged by size: 2229 1545 1428 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5299 PciI A'CATG_T Cuts at: 4198 4198 Size: 5299 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5299 PfoI T'CCnGG_A Cuts at: 2487 2549 2487 Size: 62 5237 PmeI GTTT'AAAC Cuts at: 2603 2603 Size: 5299 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3368 3400 4881 4913 3368 Size: 32 1481 32 3754 Fragments arranged by size: 3754 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5299 PsiI TTA'TAA Cuts at: 2869 2869 Size: 5299 PspOMI G'GGCC_C Cuts at: 2297 2297 Size: 5299 PstI C_TGCA'G Cuts at: 830 2178 2651 830 Size: 1348 473 3478 Fragments arranged by size: 3478 1348 473 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5299 PvuII CAG'CTG Cuts at: 2151 2151 Size: 5299 SacI G_AGCT'C Cuts at: 2069 2484 2069 Size: 415 4884 SalI G'TCGA_C Cuts at: 1936 2641 1936 Size: 705 4594 SapI GCTCTTCn'nnn_ Cuts at: 2489 2489 Size: 5299 SbfI CC_TGCA'GG Cuts at: 2651 2651 Size: 5299 ScaI AGT'ACT Cuts at: 3547 5259 3547 Size: 1712 3587 SfcI C'TryA_G Cuts at: 826 1045 2174 2647 3782 4463 4654 826 Size: 219 1129 473 1135 681 191 1471 Fragments arranged by size: 1471 1135 1129 681 473 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5299 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5299 SmaI CCC'GGG Cuts at: 1872 2626 1872 Size: 754 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3403 4304 4566 820 Size: 197 926 1460 901 262 1553 Fragments arranged by size: 1553 1460 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3090 493 Size: 2597 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5299 SphI G_CATG'C Cuts at: 1132 2657 1132 Size: 1525 3774 SspI AAT'ATT Cuts at: 5 52 3223 5 Size: 47 3171 2081 Fragments arranged by size: 3171 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5299 StyI C'CwwG_G Cuts at: 513 1065 2235 2738 513 Size: 552 1170 503 3074 Fragments arranged by size: 3074 1170 552 503 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3326 3343 3496 3681 4126 325 Size: 1255 1746 17 153 185 445 1498 Fragments arranged by size: 1746 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3545 5257 96 Size: 274 80 33 51 1443 1568 1712 138 Fragments arranged by size: 1712 1568 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2417 2449 5259 5291 2417 Size: 32 2810 32 2425 Fragments arranged by size: 2810 2425 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2573 1316 Size: 1257 4042 XbaI T'CTAG_A Cuts at: 2635 2635 Size: 5299 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5299 XmaI C'CCGG_G Cuts at: 1870 2624 1870 Size: 754 4545 XmnI GAAnn'nnTTC Cuts at: 3428 3428 Size: 5299 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3107 276 Size: 53 83 186 936 1573 2468 Fragments arranged by size: 2468 1573 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SexAI SfiI SfoI SrfI SwaI XcmI