(Circular) (Six-base) MAPSORT of: pFN21AB8749.seq Check: 7763 from: 1 to: 5959 pFN21AB8749 5959 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3769 278 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2139 3302 1937 Size: 202 1163 4594 Fragments arranged by size: 4594 1163 202 Acc65I G'GTAC_C Cuts at: 3280 3280 Size: 5959 AclI AA'CG_TT Cuts at: 1563 4086 4459 1563 Size: 2523 373 3063 Fragments arranged by size: 3063 2523 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2445 4022 5406 5674 1632 Size: 813 1577 1384 268 1917 Fragments arranged by size: 1917 1577 1384 813 268 AflII C'TTAA_G Cuts at: 820 1017 2470 820 Size: 197 1453 4309 Fragments arranged by size: 4309 1453 197 AflIII A'CryG_T Cuts at: 1203 4858 5835 1203 Size: 3655 977 1327 Fragments arranged by size: 3655 1327 977 AgeI A'CCGG_T Cuts at: 4790 4790 Size: 5959 AhdI GACnn_n'nnGTC Cuts at: 4688 4688 Size: 5959 AleI CACnn'nnGTG Cuts at: 1166 2198 1166 Size: 1032 4927 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5959 AlwNI CAG_nnn'CTG Cuts at: 3150 3702 5274 3150 Size: 552 1572 3835 Fragments arranged by size: 3835 1572 552 ApaLI G'TGCA_C Cuts at: 4016 5172 4016 Size: 1156 4803 ApoI r'AATT_y Cuts at: 1493 2913 3268 3494 3646 5755 1493 Size: 1420 355 226 152 2109 1697 Fragments arranged by size: 2109 1697 1420 355 226 152 AseI AT'TA_AT Cuts at: 160 1032 4513 160 Size: 872 3481 1606 Fragments arranged by size: 3481 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 AvaI C'yCGr_G Cuts at: 1870 1943 2907 3284 1870 Size: 73 964 377 4545 Fragments arranged by size: 4545 964 377 73 BamHI G'GATC_C Cuts at: 3289 3656 3289 Size: 367 5592 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2214 3280 4735 618 Size: 325 217 627 427 1066 1455 1842 Fragments arranged by size: 1842 1455 1066 627 427 325 217 BanII G_rGCy'C Cuts at: 1391 2542 2659 2789 3278 1391 Size: 1151 117 130 489 4072 Fragments arranged by size: 4072 1151 489 130 117 BbeI G_GCGC'C Cuts at: 2218 2218 Size: 5959 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2110 2685 928 Size: 971 211 575 4202 Fragments arranged by size: 4202 971 575 211 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3072 3106 4150 4184 1903 Size: 34 1135 34 1044 34 3678 Fragments arranged by size: 3678 1135 1044 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3851 5061 3851 Size: 1210 4749 BclI T'GATC_A Cuts at: 1548 1548 Size: 5959 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2519 4570 136 Size: 107 122 71 631 605 847 2051 1525 Fragments arranged by size: 2051 1525 847 631 605 122 107 71 BglII A'GATC_T Cuts at: 5954 5954 Size: 5959 BlpI GC'TnA_GC Cuts at: 3376 3376 Size: 5959 Bme1580I G_kGCm'C Cuts at: 1790 4020 5176 1790 Size: 2230 1156 2573 Fragments arranged by size: 2573 2230 1156 BmgBI CAC'GTC Cuts at: 1318 3096 1318 Size: 1778 4181 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3690 4648 442 Size: 416 53 83 399 24 2273 958 1753 Fragments arranged by size: 2273 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5959 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2933 2933 Size: 5959 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2444 4619 1239 Size: 1205 2175 2579 Fragments arranged by size: 2579 2175 1205 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4084 4949 5247 4084 Size: 865 298 4796 Fragments arranged by size: 4796 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2378 3161 4622 882 Size: 617 879 783 1461 2219 Fragments arranged by size: 2219 1461 879 783 617 BsaAI yAC'GTr Cuts at: 493 1204 2635 3750 493 Size: 711 1431 1115 2702 Fragments arranged by size: 2702 1431 1115 711 BsaBI GATnn'nnATC Cuts at: 5953 5953 Size: 5959 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2215 3766 4148 4783 Size: 53 83 186 936 682 1551 382 635 Cuts at: 4783 275 Size: 1451 Fragments arranged by size: 1551 1451 936 682 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4392 4790 5064 5211 5622 4392 Size: 398 274 147 411 4729 Fragments arranged by size: 4729 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5734 Fragments arranged by size: 5734 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3179 1186 Size: 1993 3966 BseYI C'CCAG_C Cuts at: 5162 5658 5162 Size: 496 5463 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3076 3150 1163 Size: 573 1340 74 3972 Fragments arranged by size: 3972 1340 573 74 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3409 4170 5198 1540 Size: 275 186 1408 761 1028 2301 Fragments arranged by size: 2301 1408 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2076 2448 4020 4105 5176 2076 Size: 372 1572 85 1071 2859 Fragments arranged by size: 2859 1572 1071 372 85 BsmI GAATG_Cn' Cuts at: 3470 3563 3470 Size: 93 5866 BsmBI CGTCTCn'nnnn_ Cuts at: 3106 3106 Size: 5959 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2076 2448 2542 2659 2789 3278 4020 Size: 399 286 372 94 117 130 489 742 Cuts at: 4020 4105 5176 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 489 399 372 286 130 117 94 85 BspEI T'CCGG_A Cuts at: 5622 5622 Size: 5959 BspHI T'CATG_A Cuts at: 3848 5578 3848 Size: 1730 4229 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3314 844 Size: 2470 3489 BsrBI CCG'CTC Cuts at: 1394 1823 3846 1394 Size: 429 2023 3507 Fragments arranged by size: 3507 2023 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4454 4628 65 Size: 1183 3206 174 1396 Fragments arranged by size: 3206 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4603 4790 1700 Size: 110 2793 187 2869 Fragments arranged by size: 2869 2793 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5959 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5959 BssSI C'ACGA_G Cuts at: 2254 4019 5031 2254 Size: 1765 1012 3182 Fragments arranged by size: 3182 1765 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5959 BstBI TT'CG_AA Cuts at: 4763 4763 Size: 5959 BstEII G'GTnAC_C Cuts at: 1184 2746 1184 Size: 1562 4397 BstXI CCAn_nnnn'nTGG Cuts at: 2200 3034 2200 Size: 834 5125 BstYI r'GATC_y Cuts at: 1972 3289 3656 4041 4058 5510 5596 5608 5954 Size: 1317 367 385 17 1452 86 12 346 Cuts at: 5954 1972 Size: 1977 Fragments arranged by size: 1977 1452 1317 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3162 3162 Size: 5959 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 5407 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5220 Fragments arranged by size: 5220 683 56 BtsI GCAGTG_nn' Cuts at: 3028 3475 4269 4289 3028 Size: 447 794 20 4698 Fragments arranged by size: 4698 794 447 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5959 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 2616 2651 529 Size: 35 2052 35 3837 Fragments arranged by size: 3837 2052 35 35 DraI TTT'AAA Cuts at: 2710 3231 3263 2710 Size: 521 32 5406 Fragments arranged by size: 5406 521 32 DraIII CAC_nnn'GTG Cuts at: 2211 2336 2211 Size: 125 5834 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4966 809 Size: 507 3650 1802 Fragments arranged by size: 3650 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2439 2511 2624 Size: 54 1425 50 275 18 609 72 113 Cuts at: 2624 3406 4295 5924 8 Size: 782 889 1629 43 Fragments arranged by size: 1629 1425 889 782 609 275 113 72 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3406 1537 Size: 275 1594 4090 Fragments arranged by size: 4090 1594 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2084 2089 3889 1353 Size: 731 5 1800 3423 Fragments arranged by size: 3423 1800 731 5 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3169 4546 4920 5066 387 Size: 1064 138 1580 1377 374 146 1280 Fragments arranged by size: 1580 1377 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2444 2445 4022 4619 5406 5674 1239 Size: 393 812 1 1577 597 787 268 1524 Fragments arranged by size: 1577 1524 812 787 597 393 268 1 EcoO109I rG'GnC_Cy Cuts at: 2364 2364 Size: 5959 EcoRI G'AATT_C Cuts at: 3268 3646 3268 Size: 378 5581 FspI TGC'GCA Cuts at: 4465 4465 Size: 5959 HaeII r_GCGC'y Cuts at: 1678 2218 5106 1678 Size: 540 2888 2531 Fragments arranged by size: 2888 2531 540 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4600 4632 4674 4706 1373 Size: 32 135 32 3028 32 42 32 2626 Fragments arranged by size: 3028 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2182 2341 2728 3303 3549 669 Size: 988 281 244 159 387 575 246 3079 Fragments arranged by size: 3079 988 575 387 281 246 244 159 HindIII A'AGCT_T Cuts at: 748 2591 748 Size: 1843 4116 HpaI GTT'AAC Cuts at: 3549 3549 Size: 5959 KasI G'GCGC_C Cuts at: 2214 2214 Size: 5959 KpnI G_GTAC'C Cuts at: 3284 3284 Size: 5959 MfeI C'AATT_G Cuts at: 3032 3558 3032 Size: 526 5433 MluI A'CGCG_T Cuts at: 5835 5835 Size: 5959 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3078 5073 5257 1305 Size: 1773 1995 184 2007 Fragments arranged by size: 2007 1995 1773 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 10 Size: 54 1425 343 681 3456 Fragments arranged by size: 3456 1425 681 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2121 2198 3917 4276 4435 518 Size: 648 955 77 1719 359 159 2042 Fragments arranged by size: 2042 1719 955 648 359 159 77 MspA1I CmG'CkG Cuts at: 1606 2130 3150 3375 4052 5200 5445 1606 Size: 524 1020 225 677 1148 245 2120 Fragments arranged by size: 2120 1148 1020 677 524 245 225 NarI GG'CG_CC Cuts at: 2215 2215 Size: 5959 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5407 NdeI CA'TA_TG Cuts at: 387 2220 387 Size: 1833 4126 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5959 NotI GC'GGCC_GC Cuts at: 3406 3406 Size: 5959 NspI r_CATG'y Cuts at: 1132 1229 3317 4862 1132 Size: 97 2088 1545 2229 Fragments arranged by size: 2229 2088 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5959 PciI A'CATG_T Cuts at: 4858 4858 Size: 5959 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5959 PmeI GTTT'AAAC Cuts at: 3263 3263 Size: 5959 PmlI CAC'GTG Cuts at: 2635 2635 Size: 5959 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4028 4060 5541 5573 4028 Size: 32 1481 32 4414 Fragments arranged by size: 4414 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 3118 1613 Size: 1505 4454 PsiI TTA'TAA Cuts at: 3529 3529 Size: 5959 PstI C_TGCA'G Cuts at: 830 3311 830 Size: 2481 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5959 PvuII CAG'CTG Cuts at: 2130 3150 2130 Size: 1020 4939 SalI G'TCGA_C Cuts at: 1936 3301 1936 Size: 1365 4594 SapI GCTCTTCn'nnn_ Cuts at: 2089 2089 Size: 5959 SbfI CC_TGCA'GG Cuts at: 3311 3311 Size: 5959 ScaI AGT'ACT Cuts at: 4207 5919 4207 Size: 1712 4247 SfcI C'TryA_G Cuts at: 826 1045 3243 3307 4442 5123 5314 826 Size: 219 2198 64 1135 681 191 1471 Fragments arranged by size: 2198 1471 1135 681 219 191 64 SfoI GGC'GCC Cuts at: 2216 2216 Size: 5959 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5959 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5959 SmaI CCC'GGG Cuts at: 1872 3286 1872 Size: 1414 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2470 4063 4964 5226 820 Size: 197 926 527 1593 901 262 1553 Fragments arranged by size: 1593 1553 926 901 527 262 197 SnaBI TAC'GTA Cuts at: 493 3750 493 Size: 3257 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5959 SphI G_CATG'C Cuts at: 1132 3317 1132 Size: 2185 3774 SspI AAT'ATT Cuts at: 5 52 3883 5 Size: 47 3831 2081 Fragments arranged by size: 3831 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5959 StyI C'CwwG_G Cuts at: 513 1065 2508 3105 3398 513 Size: 552 1443 597 293 3074 Fragments arranged by size: 3074 1443 597 552 293 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3986 4003 4156 4341 4786 325 Size: 1255 2406 17 153 185 445 1498 Fragments arranged by size: 2406 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4205 5917 96 Size: 274 80 33 51 1443 2228 1712 138 Fragments arranged by size: 2228 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2160 2192 2195 2227 5919 5951 2160 Size: 32 3 32 3692 32 2168 Fragments arranged by size: 3692 2168 32 32 32 3 Tth111I GACn'n_nGTC Cuts at: 1316 2345 1316 Size: 1029 4930 XbaI T'CTAG_A Cuts at: 2888 3295 2888 Size: 407 5552 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5959 XmaI C'CCGG_G Cuts at: 1870 3284 1870 Size: 1414 4545 XmnI GAAnn'nnTTC Cuts at: 4088 4088 Size: 5959 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3767 276 Size: 53 83 186 936 2233 2468 Fragments arranged by size: 2468 2233 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PmlI PpiI PshAI PsiI PstI PvuI PvuII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbvCI BfrBI Bpu10I BsiWI BstZ17I EcoICRI EcoNI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PfoI PpuMI PspOMI PspXI PsrI RsrII SacI SacII SanDI SexAI SfiI SrfI SwaI XcmI