(Circular) (Six-base) MAPSORT of: pFN21AB8747.seq Check: 5245 from: 1 to: 4798 pFN21AB8747 4798 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2608 278 Size: 53 83 186 936 1072 2468 Fragments arranged by size: 2468 1072 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2141 1937 Size: 204 4594 Acc65I G'GTAC_C Cuts at: 2119 2119 Size: 4798 AclI AA'CG_TT Cuts at: 1563 2925 3298 1563 Size: 1362 373 3063 Fragments arranged by size: 3063 1362 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2861 4245 4513 1632 Size: 1229 1384 268 1917 Fragments arranged by size: 1917 1384 1229 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4601 AflIII A'CryG_T Cuts at: 1203 3697 4674 1203 Size: 2494 977 1327 Fragments arranged by size: 2494 1327 977 AgeI A'CCGG_T Cuts at: 3629 3629 Size: 4798 AhdI GACnn_n'nnGTC Cuts at: 3527 3527 Size: 4798 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 4798 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 4798 AlwNI CAG_nnn'CTG Cuts at: 2541 4113 2541 Size: 1572 3226 ApaLI G'TGCA_C Cuts at: 2855 4011 2855 Size: 1156 3642 ApoI r'AATT_y Cuts at: 1493 2107 2333 2485 4594 1493 Size: 614 226 152 2109 1697 Fragments arranged by size: 2109 1697 614 226 152 AseI AT'TA_AT Cuts at: 160 1032 3352 160 Size: 872 2320 1606 Fragments arranged by size: 2320 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 4798 AvaI C'yCGr_G Cuts at: 1870 1943 2019 2123 1870 Size: 73 76 104 4545 Fragments arranged by size: 4545 104 76 73 BamHI G'GATC_C Cuts at: 2128 2495 2128 Size: 367 4431 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2119 3574 618 Size: 325 217 627 332 1455 1842 Fragments arranged by size: 1842 1455 627 332 325 217 BanII G_rGCy'C Cuts at: 1391 2117 1391 Size: 726 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 3827 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2989 3023 1903 Size: 34 1052 34 3678 Fragments arranged by size: 3678 1052 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2690 3900 2690 Size: 1210 3588 BclI T'GATC_A Cuts at: 1548 1548 Size: 4798 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3409 136 Size: 107 122 71 631 605 1737 1525 Fragments arranged by size: 1737 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 4793 4793 Size: 4798 BlpI GC'TnA_GC Cuts at: 2215 2215 Size: 4798 Bme1580I G_kGCm'C Cuts at: 1790 2859 4015 1790 Size: 1069 1156 2573 Fragments arranged by size: 2573 1156 1069 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 4798 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2529 3487 442 Size: 416 53 83 399 24 1112 958 1753 Fragments arranged by size: 1753 1112 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 4798 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3458 1239 Size: 2219 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2923 3788 4086 2923 Size: 865 298 3635 Fragments arranged by size: 3635 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3461 882 Size: 617 1962 2219 Fragments arranged by size: 2219 1962 617 BsaAI yAC'GTr Cuts at: 493 1204 2589 493 Size: 711 1385 2702 Fragments arranged by size: 2702 1385 711 BsaBI GATnn'nnATC Cuts at: 4792 4792 Size: 4798 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2605 2987 3622 275 Size: 53 83 186 936 1072 382 635 1451 Fragments arranged by size: 1451 1072 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3231 3629 3903 4050 4461 3231 Size: 398 274 147 411 3568 Fragments arranged by size: 3568 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4573 Fragments arranged by size: 4573 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 4798 BseYI C'CCAG_C Cuts at: 4001 4497 4001 Size: 496 4302 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4225 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2248 3009 4037 1540 Size: 275 186 247 761 1028 2301 Fragments arranged by size: 2301 1028 761 275 247 186 BsiHKAI G_wGCw'C Cuts at: 2859 2944 4015 2859 Size: 85 1071 3642 Fragments arranged by size: 3642 1071 85 BsmI GAATG_Cn' Cuts at: 2309 2402 2309 Size: 93 4705 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2117 2859 2944 4015 1391 Size: 399 327 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 327 85 BspEI T'CCGG_A Cuts at: 4461 4461 Size: 4798 BspHI T'CATG_A Cuts at: 2687 4417 2687 Size: 1730 3068 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2153 844 Size: 1309 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2685 1394 Size: 429 862 3507 Fragments arranged by size: 3507 862 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3293 3467 65 Size: 1183 2045 174 1396 Fragments arranged by size: 2045 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3442 3629 1700 Size: 110 1632 187 2869 Fragments arranged by size: 2869 1632 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 4798 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 4798 BssSI C'ACGA_G Cuts at: 2858 3870 2858 Size: 1012 3786 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 4798 BstBI TT'CG_AA Cuts at: 3602 3602 Size: 4798 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 4798 BstYI r'GATC_y Cuts at: 1972 2128 2495 2880 2897 4349 4435 4447 4793 Size: 156 367 385 17 1452 86 12 346 Cuts at: 4793 1972 Size: 1977 Fragments arranged by size: 1977 1452 385 367 346 156 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 3307 Fragments arranged by size: 3307 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4059 Fragments arranged by size: 4059 683 56 BtsI GCAGTG_nn' Cuts at: 2314 3108 3128 2314 Size: 794 20 3984 Fragments arranged by size: 3984 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 4798 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 4763 DraI TTT'AAA Cuts at: 2102 2102 Size: 4798 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3805 809 Size: 507 2489 1802 Fragments arranged by size: 2489 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2245 3134 4763 Size: 54 1425 50 275 18 415 889 1629 Cuts at: 4763 8 Size: 43 Fragments arranged by size: 1629 1425 889 415 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2245 1537 Size: 275 433 4090 Fragments arranged by size: 4090 433 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2728 1353 Size: 1375 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3385 3759 3905 387 Size: 1064 138 1796 374 146 1280 Fragments arranged by size: 1796 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2861 3458 4245 4513 1239 Size: 393 1229 597 787 268 1524 Fragments arranged by size: 1524 1229 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2085 2085 Size: 4798 EcoRI G'AATT_C Cuts at: 2107 2485 2107 Size: 378 4420 FspI TGC'GCA Cuts at: 3304 3304 Size: 4798 HaeII r_GCGC'y Cuts at: 1678 3945 1678 Size: 2267 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2074 2106 3439 3471 3513 Size: 32 135 32 502 32 1333 32 42 Cuts at: 3513 3545 1373 Size: 32 2626 Fragments arranged by size: 2626 1333 502 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2142 2388 669 Size: 988 281 204 246 3079 Fragments arranged by size: 3079 988 281 246 204 HindIII A'AGCT_T Cuts at: 748 748 Size: 4798 HpaI GTT'AAC Cuts at: 2388 2388 Size: 4798 KpnI G_GTAC'C Cuts at: 2123 2123 Size: 4798 MfeI C'AATT_G Cuts at: 2397 2397 Size: 4798 MluI A'CGCG_T Cuts at: 4674 4674 Size: 4798 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3912 4096 1305 Size: 2607 184 2007 Fragments arranged by size: 2607 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 2976 Fragments arranged by size: 2976 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2756 3115 3274 518 Size: 648 1590 359 159 2042 Fragments arranged by size: 2042 1590 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2214 2891 4039 4284 1606 Size: 608 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 608 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 3307 Fragments arranged by size: 3307 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 4798 NheI G'CTAG_C Cuts at: 1052 1052 Size: 4798 NotI GC'GGCC_GC Cuts at: 2245 2245 Size: 4798 NspI r_CATG'y Cuts at: 1132 1229 2156 3701 1132 Size: 97 927 1545 2229 Fragments arranged by size: 2229 1545 927 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 4798 PciI A'CATG_T Cuts at: 3697 3697 Size: 4798 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 4798 PmeI GTTT'AAAC Cuts at: 2102 2102 Size: 4798 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2867 2899 4380 4412 2867 Size: 32 1481 32 3253 Fragments arranged by size: 3253 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2085 2085 Size: 4798 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 4798 PsiI TTA'TAA Cuts at: 2368 2368 Size: 4798 PstI C_TGCA'G Cuts at: 830 2150 830 Size: 1320 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 4798 SalI G'TCGA_C Cuts at: 1936 2140 1936 Size: 204 4594 SbfI CC_TGCA'GG Cuts at: 2150 2150 Size: 4798 ScaI AGT'ACT Cuts at: 3046 4758 3046 Size: 1712 3086 SfcI C'TryA_G Cuts at: 826 1045 2146 3281 3962 4153 826 Size: 219 1101 1135 681 191 1471 Fragments arranged by size: 1471 1135 1101 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 4798 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 4798 SmaI CCC'GGG Cuts at: 1872 2021 2125 1872 Size: 149 104 4545 Fragments arranged by size: 4545 149 104 SmlI C'TyrA_G Cuts at: 820 1017 1943 2902 3803 4065 820 Size: 197 926 959 901 262 1553 Fragments arranged by size: 1553 959 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2589 493 Size: 2096 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 4798 SphI G_CATG'C Cuts at: 1132 2156 1132 Size: 1024 3774 SspI AAT'ATT Cuts at: 5 52 2722 5 Size: 47 2670 2081 Fragments arranged by size: 2670 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 4798 StyI C'CwwG_G Cuts at: 513 1065 2004 2237 513 Size: 552 939 233 3074 Fragments arranged by size: 3074 939 552 233 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2825 2842 2995 3180 3625 325 Size: 1255 1245 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1245 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3044 4756 96 Size: 274 80 33 51 1443 1067 1712 138 Fragments arranged by size: 1712 1443 1067 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 4758 4790 4758 Size: 32 4766 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 4798 XbaI T'CTAG_A Cuts at: 2134 2134 Size: 4798 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 4798 XmaI C'CCGG_G Cuts at: 1870 2019 2123 1870 Size: 149 104 4545 Fragments arranged by size: 4545 149 104 XmnI GAAnn'nnTTC Cuts at: 2927 2927 Size: 4798 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2606 276 Size: 53 83 186 936 1072 2468 Fragments arranged by size: 2468 1072 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI