Restriction map for
pFN21AB8745
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# NO CUT ENZYMES ( 131 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AfaI AgeI AhdI Alw44I ApaLI
AscI AseI AsnI Asp718I AspEI
AspHI AspI AviII AvrII BamHI
BbeI BbsI BcgI BciVI BcnI
BglII BlnI BlpI Bpu1102I BpuAI
BsaI BseAI BsiEI BsiHKAI BsiWI
BsmBI Bsp1407I BspDI BspEI BspHI
BspLU11I BspMI BsrGI BssHII BssSI
Bst1107I BstAPI BstBI BstEII BstPI
BstYI BstZ17I CelII ClaI CpoI
DraI DraIII DrdI EagI Eam1105I
EclXI Eco52I EcoNI EcoO65I EcoRI
EcoRV EcoT22I FseI FspI HincII
HindII HindIII HpaI KasI KpnI
KspI MfeI MflI MluI MroI
MunI NarI NciI NdeI NotI
NruI NsiI NspI NspV PacI
PaeR7I PflFI PflMI PinAI PmeI
PshBI Psp1406I PstI PvuI PvuII
RcaI RsaI RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SnaBI SpeI SphI Sse8387I
SspBI SspI StuI SwaI Tth111I
Van91I XbaI XhoI XhoII XmaCI
XmaI
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# ONE CUT ENZYMES ( 59 enzymes) #
###################################
AccII 283 CGCG CG^CG
AcyI 208 GGCGTC G[GA]^CG[CT]C
AflII 574 CTTAAG C^TTAAG
AflIII 18 ACGTGT A^C[GA][CT]GT
AlwNI 1132 CAGGAGCTG CAG[ACGT][ACGT][ACGT]^CTG
Aor51HI 251 AGCGCT AGC^GCT
Asp700I 135 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AvaI 144 CTCGGG C^[CT]CG[GA]G
BalI 110 TGGCCA TGG^CCA
BbrPI 17 CACGTG CAC^GTG
BbvCI 75 GCTGAGG CCTCAGC(-5/-2)
BclI 1118 TGATCA T^GATCA
BfaI 5 CTAG C^TAG
BfrI 574 CTTAAG C^TTAAG
BglI 112 GCCAGCATGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaAI 17 CACGTG [CT]AC^GT[GA]
BsaBI 759 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaHI 208 GGCGTC G[GA]^CG[CT]C
BsaWI 512 ACCGGA [AT]^CCGG[AT]
BseRI 235 GAGGAG GAGGAG(10/8)
BsgI 460 GTGCAG GTGCAG(16/14)
BsmI 42 GAATGC GAATGC(1/-1)
BsoBI 144 CTCGGG C^[CT]CG[GA]G
BsrBI 610 GAGCGG CCGCTC(-3/-3)
BsrFI 32 GCCGGC [GA]^CCGG[CT]
BstUI 283 CGCG CG^CG
CfoI 252 GCGC GCG^C
Cfr10I 32 GCCGGC [GA]^CCGG[CT]
DsaI 390 CCATGG C^C[GA][CT]GG
EaeI 110 TGGCCA [CT]^GGCC[GA]
EarI 655 GAAGAG CTCTTC(1/4)
Eco47III 251 AGCGCT AGC^GCT
FauI 281 CCCGC CCCGC(4/6)
FbaI 1118 TGATCA T^GATCA
HaeII 251 AGCGCT [GA]GCGC^[CT]
HhaI 252 GCGC GCG^C
Hin1I 208 GGCGTC G[GA]^CG[CT]C
HinP1I 252 GCGC G^CGC
Ksp632I 655 GAAGAG CTCTTC(1/4)
MaeI 5 CTAG C^TAG
MaeII 18 ACGT A^CGT
MamI 759 GATAGTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MluNI 110 TGGCCA TGG^CCA
MscI 110 TGGCCA TGG^CCA
MslI 291 CATGCGTGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspA1I 1035 CCGCTG C[AC]G^C[GT]G
MvnI 283 CGCG CG^CG
NaeI 32 GCCGGC GCC^GGC
NcoI 390 CCATGG C^CATGG
NgoMIV 32 GCCGGC G^CCGGC
NheI 4 GCTAGC G^CTAGC
PmaCI 17 CACGTG CAC^GTG
PmlI 17 CACGTG CAC^GTG
PshAI 195 GACCCAAGTC GAC[ACGT][ACGT]^[ACGT][ACGT]GTC
TaqI 809 TCGA T^CGA
TfiI 925 GATTC G^A[AT]TC
TthHB8I 809 TCGA T^CGA
XcmI 856 CCACTTCCATCCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XmnI 135 GAAGTTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 17 enzymes) #
###################################
AcsI 397 AAATTT [GA]^AATT[CT]
1113 GAATTT
AlwI 447 GATCC GGATC(4/5)
831 GATCC
AocI 267 CCTTAGG CC^T[ACGT]AGG
901 CCTCAGG
ApaI 62 GGGCCC GGGCC^C
273 GGGCCC
ApoI 397 AAATTT [GA]^AATT[CT]
1113 GAATTT
BanI 156 GGTGCC G^G[CT][GA]CC
871 GGTGCC
BsmFI 557 GGGAC GGGAC(10/14)
81 GTCCC
BsrDI 12 CATTGC GCAATG(2/0)
782 GCAATG
BsrI 188 ACTGG ACTGG(1/-1)
962 CCAGT
Bsu36I 267 CCTTAGG CC^T[ACGT]AGG
901 CCTCAGG
Eco81I 267 CCTTAGG CC^T[ACGT]AGG
901 CCTCAGG
HapII 33 CCGG C^CGG
513 CCGG
HgaI 209 GCGTC GACGC(5/10)
690 GCGTC
HpaII 33 CCGG C^CGG
513 CCGG
HphI 544 GGTGA GGTGA(8/7)
1051 GGTGA
MboII 172 GAAGA GAAGA(8/7)
655 GAAGA
MspI 33 CCGG C^CGG
513 CCGG
#########################################
# 3 OR MORE CUT ENZYMES ( 51 enzymes) #
#########################################
AciI AluI AvaII BanII BbvI
BmyI BpmI BsaJI BsiYI BslI
BsmAI Bsp1286I BssKI BstF5I BstNI
BstXI Cac8I Cfr13I DdeI DpnII
DraII EcoO109I EcoRII EcoT14I Fnu4HI
FokI HaeIII HinfI ItaI MaeIII
MboI MnlI MseI MvaI MwoI
NdeII NlaIII NlaIV PleI PpuMI
Sau3AI Sau96I ScrFI SfaNI SmlI
StyI Tru9I TseI Tsp45I Tsp509I
TspRI