(Circular) (Six-base) MAPSORT of: pFN21AB8745.seq Check: 7014 from: 1 to: 5911 pFN21AB8745 5911 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3721 278 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3254 1937 Size: 1317 4594 Acc65I G'GTAC_C Cuts at: 3232 3232 Size: 5911 AclI AA'CG_TT Cuts at: 1563 4038 4411 1563 Size: 2475 373 3063 Fragments arranged by size: 3063 2475 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2291 3974 5358 5626 1632 Size: 659 1683 1384 268 1917 Fragments arranged by size: 1917 1683 1384 659 268 AfeI AGC'GCT Cuts at: 2258 2258 Size: 5911 AflII C'TTAA_G Cuts at: 820 1017 2579 820 Size: 197 1562 4152 Fragments arranged by size: 4152 1562 197 AflIII A'CryG_T Cuts at: 1203 2023 4810 5787 1203 Size: 820 2787 977 1327 Fragments arranged by size: 2787 1327 977 820 AgeI A'CCGG_T Cuts at: 4742 4742 Size: 5911 AhdI GACnn_n'nnGTC Cuts at: 4640 4640 Size: 5911 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5911 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5911 AlwNI CAG_nnn'CTG Cuts at: 3142 3654 5226 3142 Size: 512 1572 3827 Fragments arranged by size: 3827 1572 512 ApaI G_GGCC'C Cuts at: 2071 2282 2071 Size: 211 5700 ApaLI G'TGCA_C Cuts at: 3968 5124 3968 Size: 1156 4755 ApoI r'AATT_y Cuts at: 1493 2402 3118 3220 3446 3598 5707 1493 Size: 909 716 102 226 152 2109 1697 Fragments arranged by size: 2109 1697 909 716 226 152 102 AseI AT'TA_AT Cuts at: 160 1032 4465 160 Size: 872 3433 1606 Fragments arranged by size: 3433 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 AvaI C'yCGr_G Cuts at: 1870 1943 2149 3236 1870 Size: 73 206 1087 4545 Fragments arranged by size: 4545 1087 206 73 BamHI G'GATC_C Cuts at: 3241 3608 3241 Size: 367 5544 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2161 2876 3232 4687 618 Size: 325 217 627 374 715 356 1455 1842 Fragments arranged by size: 1842 1455 715 627 374 356 325 217 BanII G_rGCy'C Cuts at: 1391 2071 2282 2756 2953 3230 1391 Size: 680 211 474 197 277 4072 Fragments arranged by size: 4072 680 474 277 211 197 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4940 BbvCI CC'TCA_GC Cuts at: 2081 2081 Size: 5911 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4102 4136 1903 Size: 34 2165 34 3678 Fragments arranged by size: 3678 2165 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3803 5013 3803 Size: 1210 4701 BclI T'GATC_A Cuts at: 1548 3123 1548 Size: 1575 4336 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2123 4522 136 Size: 107 122 71 631 605 451 2399 1525 Fragments arranged by size: 2399 1525 631 605 451 122 107 71 BglII A'GATC_T Cuts at: 5906 5906 Size: 5911 BlpI GC'TnA_GC Cuts at: 3328 3328 Size: 5911 Bme1580I G_kGCm'C Cuts at: 1790 2071 2282 2881 3972 5128 1790 Size: 281 211 599 1091 1156 2573 Fragments arranged by size: 2573 1156 1091 599 281 211 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5911 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3642 4600 442 Size: 416 53 83 399 24 2225 958 1753 Fragments arranged by size: 2225 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2013 1056 Size: 957 4954 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2455 2455 Size: 5911 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2456 2654 3119 4571 1239 Size: 1217 198 465 1452 2579 Fragments arranged by size: 2579 1452 1217 465 198 Bpu10I CC'TnA_GC Cuts at: 2081 2081 Size: 5911 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2237 2409 4036 4901 5199 2237 Size: 172 1627 865 298 2949 Fragments arranged by size: 2949 1627 865 298 172 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4574 882 Size: 617 3075 2219 Fragments arranged by size: 3075 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2024 3702 493 Size: 711 820 1678 2702 Fragments arranged by size: 2702 1678 820 711 BsaBI GATnn'nnATC Cuts at: 2768 5905 2768 Size: 3137 2774 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2214 3718 4100 4735 Size: 53 83 186 936 681 1504 382 635 Cuts at: 4735 275 Size: 1451 Fragments arranged by size: 1504 1451 936 681 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2517 4344 4742 5016 5163 5574 2517 Size: 1827 398 274 147 411 2854 Fragments arranged by size: 2854 1827 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5686 Fragments arranged by size: 5686 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2255 1186 Size: 1069 4842 BseYI C'CCAG_C Cuts at: 5114 5610 5114 Size: 496 5415 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2486 1163 Size: 573 750 4588 Fragments arranged by size: 4588 750 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3361 4122 5150 1540 Size: 275 186 1360 761 1028 2301 Fragments arranged by size: 2301 1360 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 3972 4057 5128 3972 Size: 85 1071 4755 Fragments arranged by size: 4755 1071 85 BsmI GAATG_Cn' Cuts at: 2053 3422 3515 2053 Size: 1369 93 4449 Fragments arranged by size: 4449 1369 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2071 2282 2756 2881 2953 3230 3972 Size: 399 281 211 474 125 72 277 742 Cuts at: 3972 4057 5128 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 474 399 281 277 211 125 85 72 BspEI T'CCGG_A Cuts at: 5574 5574 Size: 5911 BspHI T'CATG_A Cuts at: 3800 5530 3800 Size: 1730 4181 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3266 844 Size: 2422 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2617 3798 1394 Size: 429 794 1181 3507 Fragments arranged by size: 3507 1181 794 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2016 2794 4406 4580 65 Size: 1183 768 778 1612 174 1396 Fragments arranged by size: 1612 1396 1183 778 768 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2037 4555 4742 1700 Size: 110 227 2518 187 2869 Fragments arranged by size: 2869 2518 227 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5911 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5911 BssSI C'ACGA_G Cuts at: 3971 4983 3971 Size: 1012 4899 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5911 BstBI TT'CG_AA Cuts at: 4715 4715 Size: 5911 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5911 BstXI CCAn_nnnn'nTGG Cuts at: 2210 2359 2632 2210 Size: 149 273 5489 Fragments arranged by size: 5489 273 149 BstYI r'GATC_y Cuts at: 1972 3241 3608 3993 4010 5462 5548 5560 5906 Size: 1269 367 385 17 1452 86 12 346 Cuts at: 5906 1972 Size: 1977 Fragments arranged by size: 1977 1452 1269 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2273 2907 2273 Size: 634 5277 BtgI C'CryG_G Cuts at: 513 1065 2004 2395 513 Size: 552 939 391 4029 Fragments arranged by size: 4029 939 552 391 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5172 Fragments arranged by size: 5172 683 56 BtsI GCAGTG_nn' Cuts at: 2389 2782 3427 4221 4241 2389 Size: 393 645 794 20 4059 Fragments arranged by size: 4059 794 645 393 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5911 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5876 DraI TTT'AAA Cuts at: 3215 3215 Size: 5911 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4918 809 Size: 507 3602 1802 Fragments arranged by size: 3602 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2115 3358 4247 Size: 54 1425 50 275 18 285 1243 889 Cuts at: 4247 5876 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1243 889 285 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3358 1537 Size: 275 1546 4090 Fragments arranged by size: 4090 1546 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2655 3841 1353 Size: 1302 1186 3423 Fragments arranged by size: 3423 1302 1186 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4498 4872 5018 387 Size: 1064 138 2909 374 146 1280 Fragments arranged by size: 2909 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2291 2456 2654 3119 3974 4571 5358 Size: 393 659 165 198 465 855 597 787 Cuts at: 5358 5626 1239 Size: 268 1524 Fragments arranged by size: 1524 855 787 659 597 465 393 268 198 165 EcoO109I rG'GnC_Cy Cuts at: 2085 2278 2279 2563 2854 2085 Size: 193 1 284 291 5142 Fragments arranged by size: 5142 291 284 193 1 EcoRI G'AATT_C Cuts at: 3220 3598 3220 Size: 378 5533 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2459 2459 Size: 5911 FspI TGC'GCA Cuts at: 4417 4417 Size: 5911 HaeII r_GCGC'y Cuts at: 1678 2260 5058 1678 Size: 582 2798 2531 Fragments arranged by size: 2798 2531 582 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3115 3147 4552 4584 4626 Size: 32 135 32 1543 32 1405 32 42 Cuts at: 4626 4658 1373 Size: 32 2626 Fragments arranged by size: 2626 1543 1405 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3255 3501 669 Size: 988 281 1317 246 3079 Fragments arranged by size: 3079 1317 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5911 HpaI GTT'AAC Cuts at: 3501 3501 Size: 5911 KpnI G_GTAC'C Cuts at: 3236 3236 Size: 5911 MfeI C'AATT_G Cuts at: 3510 3510 Size: 5911 MluI A'CGCG_T Cuts at: 5787 5787 Size: 5911 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2593 5025 5209 1305 Size: 1288 2432 184 2007 Fragments arranged by size: 2432 2007 1288 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2117 10 Size: 54 1425 343 285 3804 Fragments arranged by size: 3804 1425 343 285 54 MslI CAynn'nnrTG Cuts at: 518 1166 2300 3869 4228 4387 518 Size: 648 1134 1569 359 159 2042 Fragments arranged by size: 2042 1569 1134 648 359 159 MspA1I CmG'CkG Cuts at: 1606 3042 3327 4004 5152 5397 1606 Size: 1436 285 677 1148 245 2120 Fragments arranged by size: 2120 1436 1148 677 285 245 NaeI GCC'GGC Cuts at: 2039 2039 Size: 5911 NcoI C'CATG_G Cuts at: 513 1065 2004 2395 513 Size: 552 939 391 4029 Fragments arranged by size: 4029 939 552 391 NdeI CA'TA_TG Cuts at: 387 387 Size: 5911 NgoMIV G'CCGG_C Cuts at: 2037 2037 Size: 5911 NheI G'CTAG_C Cuts at: 1052 2009 1052 Size: 957 4954 NotI GC'GGCC_GC Cuts at: 3358 3358 Size: 5911 NspI r_CATG'y Cuts at: 1132 1229 3269 4814 1132 Size: 97 2040 1545 2229 Fragments arranged by size: 2229 2040 1545 97 PasI CC'CwG_GG Cuts at: 1348 2089 1348 Size: 741 5170 PciI A'CATG_T Cuts at: 4810 4810 Size: 5911 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5911 PfoI T'CCnGG_A Cuts at: 3135 3135 Size: 5911 PmeI GTTT'AAAC Cuts at: 3215 3215 Size: 5911 PmlI CAC'GTG Cuts at: 2024 2024 Size: 5911 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3980 4012 5493 5525 3980 Size: 32 1481 32 4366 Fragments arranged by size: 4366 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2085 2563 2854 2085 Size: 478 291 5142 Fragments arranged by size: 5142 478 291 PshAI GACnn'nnGTC Cuts at: 1613 2204 1613 Size: 591 5320 PsiI TTA'TAA Cuts at: 3481 3481 Size: 5911 PspOMI G'GGCC_C Cuts at: 2067 2278 2067 Size: 211 5700 PstI C_TGCA'G Cuts at: 830 3263 830 Size: 2433 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5911 SalI G'TCGA_C Cuts at: 1936 3253 1936 Size: 1317 4594 SbfI CC_TGCA'GG Cuts at: 3263 3263 Size: 5911 ScaI AGT'ACT Cuts at: 4159 5871 4159 Size: 1712 4199 SfcI C'TryA_G Cuts at: 826 1045 3259 4394 5075 5266 826 Size: 219 2214 1135 681 191 1471 Fragments arranged by size: 2214 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5911 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5911 SmaI CCC'GGG Cuts at: 1872 3238 1872 Size: 1366 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2252 2424 2579 4015 4916 5178 Size: 197 926 309 172 155 1436 901 262 Cuts at: 5178 820 Size: 1553 Fragments arranged by size: 1553 1436 926 901 309 262 197 172 155 SnaBI TAC'GTA Cuts at: 493 3702 493 Size: 3209 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5911 SphI G_CATG'C Cuts at: 1132 3269 1132 Size: 2137 3774 SspI AAT'ATT Cuts at: 5 52 3835 5 Size: 47 3783 2081 Fragments arranged by size: 3783 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5911 StyI C'CwwG_G Cuts at: 513 1065 2004 2063 2170 2395 3350 513 Size: 552 939 59 107 225 955 3074 Fragments arranged by size: 3074 955 939 552 225 107 59 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2093 3938 3955 4108 4293 4738 325 Size: 1255 513 1845 17 153 185 445 1498 Fragments arranged by size: 1845 1498 1255 513 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4157 5869 96 Size: 274 80 33 51 1443 2180 1712 138 Fragments arranged by size: 2180 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5871 5903 5871 Size: 32 5879 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5911 XbaI T'CTAG_A Cuts at: 3247 3247 Size: 5911 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2868 2868 Size: 5911 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5911 XmaI C'CCGG_G Cuts at: 1870 3236 1870 Size: 1366 4545 XmnI GAAnn'nnTTC Cuts at: 2144 4040 2144 Size: 1896 4015 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3719 276 Size: 53 83 186 936 2185 2468 Fragments arranged by size: 2468 2185 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BfrBI BsiWI BsmBI BstZ17I DraIII EcoICRI EcoNI EcoRV FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI