Restriction map for
pFN21AB8745
(Linear) (Six-base) MAPSORT of: KIBB8745.seq Check: 3713 from: 1 to: 1206
KIBB8745.orf
With 182 enzymes: SgfI *
March 3, 2008 11:08 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 1206
Size: 286 920
AfeI AGC'GCT
Cuts at: 0 253 1206
Size: 253 953
AflII C'TTAA_G
Cuts at: 0 574 1206
Size: 574 632
AflIII A'CryG_T
Cuts at: 0 18 1206
Size: 18 1188
AlwNI CAG_nnn'CTG
Cuts at: 0 1137 1206
Size: 1137 69
ApaI G_GGCC'C
Cuts at: 0 66 277 1206
Size: 66 211 929
Fragments arranged by size:
929 211 66
ApoI r'AATT_y
Cuts at: 0 397 1113 1206
Size: 397 716 93
Fragments arranged by size:
716 397 93
AvaI C'yCGr_G
Cuts at: 0 144 1206
Size: 144 1062
BanI G'GyrC_C
Cuts at: 0 156 871 1206
Size: 156 715 335
Fragments arranged by size:
715 335 156
BanII G_rGCy'C
Cuts at: 0 66 277 751 948 1206
Size: 66 211 474 197 258
Fragments arranged by size:
474 258 211 197 66
BbvCI CC'TCA_GC
Cuts at: 0 76 1206
Size: 76 1130
BclI T'GATC_A
Cuts at: 0 1118 1206
Size: 1118 88
BglI GCCn_nnn'nGGC
Cuts at: 0 118 1206
Size: 118 1088
Bme1580I G_kGCm'C
Cuts at: 0 66 277 876 1206
Size: 66 211 599 330
Fragments arranged by size:
599 330 211 66
BmtI G_CTAG'C
Cuts at: 0 8 1206
Size: 8 1198
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 450 1206
Size: 450 756
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 451 649 1114 1206
Size: 451 198 465 92
Fragments arranged by size:
465 451 198 92
Bpu10I CC'TnA_GC
Cuts at: 0 76 1206
Size: 76 1130
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 232 404 1206
Size: 232 172 802
Fragments arranged by size:
802 232 172
BsaAI yAC'GTr
Cuts at: 0 19 1206
Size: 19 1187
BsaBI GATnn'nnATC
Cuts at: 0 763 1206
Size: 763 443
BsaHI Gr'CG_yC
Cuts at: 0 209 1206
Size: 209 997
BsaWI w'CCGG_w
Cuts at: 0 512 1206
Size: 512 694
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 250 1206
Size: 250 956
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 481 1206
Size: 481 725
BsmI GAATG_Cn'
Cuts at: 0 48 1206
Size: 48 1158
Bsp1286I G_dGCh'C
Cuts at: 0 66 277 751 876 948 1206
Size: 66 211 474 125 72 258
Fragments arranged by size:
474 258 211 125 72 66
BsrBI CCG'CTC
Cuts at: 0 612 1206
Size: 612 594
BsrDI GCAATG_nn'
Cuts at: 0 11 789 1206
Size: 11 778 417
Fragments arranged by size:
778 417 11
BsrFI r'CCGG_y
Cuts at: 0 32 1206
Size: 32 1174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 205 354 627 1206
Size: 205 149 273 579
Fragments arranged by size:
579 273 205 149
Bsu36I CC'TnA_GG
Cuts at: 0 268 902 1206
Size: 268 634 304
Fragments arranged by size:
634 304 268
BtgI C'CryG_G
Cuts at: 0 390 1206
Size: 390 816
BtsI GCAGTG_nn'
Cuts at: 0 384 777 1206
Size: 384 393 429
Fragments arranged by size:
429 393 384
EaeI y'GGCC_r
Cuts at: 0 110 1206
Size: 110 1096
EarI CTCTTCn'nnn_
Cuts at: 0 650 1206
Size: 650 556
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 286 451 649 1114 1206
Size: 286 165 198 465 92
Fragments arranged by size:
465 286 198 165 92
EcoO109I rG'GnC_Cy
Cuts at: 0 80 273 274 558 849 1206
Size: 80 193 1 284 291 357
Fragments arranged by size:
357 291 284 193 80 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 454 1206
Size: 454 752
HaeII r_GCGC'y
Cuts at: 0 255 1206
Size: 255 951
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1110 1142 1206
Size: 1110 32 64
Fragments arranged by size:
1110 64 32
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 588 1206
Size: 588 618
MscI TGG'CCA
Cuts at: 0 112 1206
Size: 112 1094
MslI CAynn'nnrTG
Cuts at: 0 295 1206
Size: 295 911
MspA1I CmG'CkG
Cuts at: 0 1037 1206
Size: 1037 169
NaeI GCC'GGC
Cuts at: 0 34 1206
Size: 34 1172
NcoI C'CATG_G
Cuts at: 0 390 1206
Size: 390 816
NgoMIV G'CCGG_C
Cuts at: 0 32 1206
Size: 32 1174
NheI G'CTAG_C
Cuts at: 0 4 1206
Size: 4 1202
PasI CC'CwG_GG
Cuts at: 0 84 1206
Size: 84 1122
PfoI T'CCnGG_A
Cuts at: 0 1130 1206
Size: 1130 76
PmlI CAC'GTG
Cuts at: 0 19 1206
Size: 19 1187
PpuMI rG'GwC_Cy
Cuts at: 0 80 558 849 1206
Size: 80 478 291 357
Fragments arranged by size:
478 357 291 80
PshAI GACnn'nnGTC
Cuts at: 0 199 1206
Size: 199 1007
PspOMI G'GGCC_C
Cuts at: 0 62 273 1206
Size: 62 211 933
Fragments arranged by size:
933 211 62
SmlI C'TyrA_G
Cuts at: 0 247 419 574 1206
Size: 247 172 155 632
Fragments arranged by size:
632 247 172 155
StyI C'CwwG_G
Cuts at: 0 58 165 390 1206
Size: 58 107 225 816
Fragments arranged by size:
816 225 107 58
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 88 1206
Size: 88 1118
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 863 1206
Size: 863 343
XmnI GAAnn'nnTTC
Cuts at: 0 139 1206
Size: 139 1067
Enzymes that do cut:
AcuI AfeI AflII AflIII AlwNI ApaI ApoI AvaI
BanI BanII BbvCI BclI BglI Bme1580I BmtI BplI
BpmI Bpu10I BpuEI BsaAI BsaBI BsaHI BsaWI BseRI
BsgI BsmI Bsp1286I BsrBI BsrDI BsrFI BstXI Bsu36I
BtgI BtsI EaeI EarI Eco57MI EcoO109I FalI HaeII
Hin4I MmeI MscI MslI MspA1I NaeI NcoI NgoMIV
NheI PasI PfoI PmlI PpuMI PshAI PspOMI SmlI
StyI TaqII XcmI XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AgeI AhdI AleI
AlfI AloI ApaLI AscI AseI AsiSI AvrII BaeI
BamHI BbeI BbsI BcgI BciVI BfrBI BglII BlpI
BmgBI BmrI BsaI BsaXI BseYI BsiEI BsiHKAI BsiWI
BsmBI BspEI BspHI BspMI BsrGI BssHII BssSI BstAPI
BstBI BstEII BstYI BstZ17I BtgZI ClaI CspCI DraI
DraIII DrdI EagI EciI EcoICRI EcoNI EcoRI EcoRV
FseI FspI FspAI HincII HindIII HpaI KasI KpnI
MfeI MluI NarI NdeI NotI NruI NsiI NspI
PacI PciI PflMI PmeI PpiI PsiI PspXI PsrI
PstI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI ScaI SexAI SfcI SfiI SfoI SgfI
SgrAI SmaI SnaBI SpeI SphI SrfI SspI StuI
SwaI TatI TstI Tth111I XbaI XhoI XmaI ZraI