Restriction map for
pFN21AB8744
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# NO CUT ENZYMES ( 175 enzymes) #
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AatII Acc65I AccI AccIII AclI
AcsI AcyI AflII AflIII AgeI
Alw44I AlwI Aor51HI ApaI ApaLI
ApoI AscI AseI AsnI Asp700I
Asp718I AspI AviII AvrII BalI
BamHI BanI BbeI BcgI BciVI
BclI BfaI BfrI BglI BglII
BlnI BlpI Bpu1102I BsaBI BsaHI
BsaI BsaWI BseAI BsiEI BsiWI
BsmFI BsmI Bsp1407I BspDI BspEI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BsrI BssHII BssSI Bst1107I
BstBI BstEII BstF5I BstPI BstXI
BstYI BstZ17I CelII Cfr10I ClaI
CpoI DraI DraII DraIII DrdI
DsaI EaeI EagI EarI EclXI
Eco47III Eco52I EcoNI EcoO109I EcoO65I
EcoRI EcoRV EcoT22I FbaI FokI
FseI FspI Hin1I HincII HindII
HindIII HpaI HphI KasI KpnI
Ksp632I KspI MaeI MaeIII MamI
MfeI MflI MluI MluNI MroI
MscI MseI MspA1I MunI NaeI
NarI NcoI NdeI NgoMIV NheI
NlaIII NotI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PpuMI PshAI PshBI
Psp1406I PstI PvuI PvuII RcaI
RsrII SacII SalI SapI SbfI
ScaI SexAI SfcI SfiI SfoI
SfuI SgrAI SmaI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
SwaI TfiI Tru9I Tsp45I Tsp509I
TspRI Tth111I Van91I XbaI XcmI
XhoI XhoII XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 45 enzymes) #
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AfaI 147 GTAC GT^AC
AhdI 112 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwNI 76 CAGTTCCTG CAG[ACGT][ACGT][ACGT]^CTG
AocI 34 CCTGAGG CC^T[ACGT]AGG
AspEI 112 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaII 155 GGACC G^G[AT]CC
BbrPI 241 CACGTG CAC^GTG
BbsI 375 GTCTTC GAAGAC(2/6)
BbvCI 298 GCTGAGG CCTCAGC(-5/-2)
BbvI 246 GCAGC GCAGC(8/12)
BcnI 87 CCCGG CC^[GC]GG
BpmI 52 CTGGAG CTGGAG(16/14)
BpuAI 375 GTCTTC GAAGAC(2/6)
BsaAI 241 CACGTG [CT]AC^GT[GA]
BsgI 244 GTGCAG GTGCAG(16/14)
BsiYI 81 CCTGCTCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 81 CCTGCTCCCGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmAI 120 GTCTC GTCTC(1/5)
BsmBI 119 CGTCTC CGTCTC(1/5)
BstAPI 75 GCAGTTCCTGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Bsu36I 34 CCTGAGG CC^T[ACGT]AGG
DpnII 198 GATC ^GATC
Eam1105I 112 GACATCGCGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 34 CCTGAGG CC^T[ACGT]AGG
EcoT14I 311 CCAAGG C^C[AT][AT]GG
FauI 141 GCGGG CCCGC(4/6)
HapII 88 CCGG C^CGG
HgaI 118 GCGTC GACGC(5/10)
HinfI 373 GAGTC G^A[ACGT]TC
HpaII 88 CCGG C^CGG
MaeII 242 ACGT A^CGT
MboI 198 GATC ^GATC
MslI 250 CACCCGAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
MspI 88 CCGG C^CGG
NciI 87 CCCGG CC^[GC]GG
NdeII 198 GATC ^GATC
PleI 373 GAGTC GAGTC(4/5)
PmaCI 241 CACGTG CAC^GTG
PmlI 241 CACGTG CAC^GTG
RsaI 147 GTAC GT^AC
SacI 346 GAGCTC GAGCT^C
Sau3AI 198 GATC ^GATC
StuI 215 AGGCCT AGG^CCT
StyI 311 CCAAGG C^C[AT][AT]GG
TseI 246 GCAGC G^C[AT]GC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AluI 167 AGCT AG^CT
347 AGCT
AspHI 133 GAGCAC G[AT]GC[AT]^C
346 GAGCTC
AvaI 58 CCCGAG C^[CT]CG[GA]G
252 CCCGAG
BanII 55 GAGCCC G[GA]GC[CT]^C
346 GAGCTC
BsiHKAI 133 GAGCAC G[AT]GC[AT]^C
346 GAGCTC
BsoBI 58 CCCGAG C^[CT]CG[GA]G
252 CCCGAG
BspMI 149 ACCTGC ACCTGC(4/8)
207 GCAGGT
BstNI 9 CCTGG CC^[AT]GG
158 CCAGG
Cfr13I 5 GGCCC G^G[ACGT]CC
155 GGACC
DdeI 35 CTGAG C^T[ACGT]AG
299 CTGAG
EcoRII 9 CCTGG ^CC[AT]GG
158 CCAGG
Fnu4HI 3 GCGGC GC^[ACGT]GC
246 GCAGC
HaeII 30 AGCGCC [GA]GCGC^[CT]
229 AGCGCC
HaeIII 5 GGCC GG^CC
216 GGCC
ItaI 3 GCGGC GC^[ACGT]GC
246 GCAGC
MboII 324 GAAGA GAAGA(8/7)
376 TCTTC
MvaI 9 CCTGG CC^[AT]GG
158 CCAGG
NlaIV 5 GGCCCC GG[ACGT]^[ACGT]CC
54 GGAGCC
Sau96I 5 GGCCC G^G[ACGT]CC
155 GGACC
SfaNI 102 GATGC GCATC(5/9)
336 GATGC
TaqI 71 TCGA T^CGA
344 TCGA
TthHB8I 71 TCGA T^CGA
344 TCGA
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# 3 OR MORE CUT ENZYMES ( 16 enzymes) #
#########################################
AccII AciI BmyI BsaJI BseRI
Bsp1286I BssKI BstUI Cac8I CfoI
HhaI HinP1I MnlI MvnI MwoI
ScrFI