(Circular) (Six-base) MAPSORT of: pFN21AB8743.seq Check: 9149 from: 1 to: 5074 pFN21AB8743 5074 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2884 278 Size: 53 83 186 936 1348 2468 Fragments arranged by size: 2468 1348 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2417 1937 Size: 480 4594 Acc65I G'GTAC_C Cuts at: 2395 2395 Size: 5074 AclI AA'CG_TT Cuts at: 1563 3201 3574 1563 Size: 1638 373 3063 Fragments arranged by size: 3063 1638 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3137 4521 4789 1632 Size: 1505 1384 268 1917 Fragments arranged by size: 1917 1505 1384 268 AfeI AGC'GCT Cuts at: 2243 2243 Size: 5074 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 4877 AflIII A'CryG_T Cuts at: 1203 3973 4950 1203 Size: 2770 977 1327 Fragments arranged by size: 2770 1327 977 AgeI A'CCGG_T Cuts at: 3905 3905 Size: 5074 AhdI GACnn_n'nnGTC Cuts at: 2175 3803 2175 Size: 1628 3446 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5074 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5074 AlwNI CAG_nnn'CTG Cuts at: 2817 4389 2817 Size: 1572 3502 ApaI G_GGCC'C Cuts at: 2294 2294 Size: 5074 ApaLI G'TGCA_C Cuts at: 3131 4287 3131 Size: 1156 3918 ApoI r'AATT_y Cuts at: 1493 2383 2609 2761 4870 1493 Size: 890 226 152 2109 1697 Fragments arranged by size: 2109 1697 890 226 152 AseI AT'TA_AT Cuts at: 160 1032 3628 160 Size: 872 2596 1606 Fragments arranged by size: 2596 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5074 AvaI C'yCGr_G Cuts at: 1870 1943 2017 2237 2399 1870 Size: 73 74 220 162 4545 Fragments arranged by size: 4545 220 162 74 73 BamHI G'GATC_C Cuts at: 2404 2771 2404 Size: 367 4707 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2346 2352 2395 3850 618 Size: 325 217 627 559 6 43 1455 1842 Fragments arranged by size: 1842 1455 627 559 325 217 43 6 BanII G_rGCy'C Cuts at: 1391 2053 2294 2393 1391 Size: 662 241 99 4072 Fragments arranged by size: 4072 662 241 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4103 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3265 3299 1903 Size: 34 1328 34 3678 Fragments arranged by size: 3678 1328 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2966 4176 2966 Size: 1210 3864 BclI T'GATC_A Cuts at: 1548 1548 Size: 5074 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2021 3685 136 Size: 107 122 71 631 605 349 1664 1525 Fragments arranged by size: 1664 1525 631 605 349 122 107 71 BglII A'GATC_T Cuts at: 5069 5069 Size: 5074 BlpI GC'TnA_GC Cuts at: 2491 2491 Size: 5074 Bme1580I G_kGCm'C Cuts at: 1790 2294 2357 3135 4291 1790 Size: 504 63 778 1156 2573 Fragments arranged by size: 2573 1156 778 504 63 BmgBI CAC'GTC Cuts at: 1318 2125 1318 Size: 807 4267 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2045 2805 3763 Size: 416 53 83 399 24 628 760 958 Cuts at: 3763 442 Size: 1753 Fragments arranged by size: 1753 958 760 628 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5074 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3734 1239 Size: 2495 2579 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3199 4064 4362 3199 Size: 865 298 3911 Fragments arranged by size: 3911 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3737 882 Size: 617 2238 2219 Fragments arranged by size: 2238 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2865 493 Size: 711 1661 2702 Fragments arranged by size: 2702 1661 711 BsaBI GATnn'nnATC Cuts at: 5068 5068 Size: 5074 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2009 2151 2299 2881 Size: 53 83 186 936 476 142 148 582 Cuts at: 2881 3263 3898 275 Size: 382 635 1451 Fragments arranged by size: 1451 936 635 582 476 382 186 148 142 83 53 BsaWI w'CCGG_w Cuts at: 3507 3905 4179 4326 4737 3507 Size: 398 274 147 411 3844 Fragments arranged by size: 3844 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 4849 Fragments arranged by size: 4849 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2179 1186 Size: 993 4081 BseYI C'CCAG_C Cuts at: 2067 2250 4277 4773 2067 Size: 183 2027 496 2368 Fragments arranged by size: 2368 2027 496 183 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 4501 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2057 2365 2524 3285 4313 1540 Size: 275 186 56 308 159 761 1028 2301 Fragments arranged by size: 2301 1028 761 308 275 186 159 56 BsiHKAI G_wGCw'C Cuts at: 3135 3220 4291 3135 Size: 85 1071 3918 Fragments arranged by size: 3918 1071 85 BsmI GAATG_Cn' Cuts at: 2585 2678 2585 Size: 93 4981 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2053 2294 2357 2393 3135 3220 4291 Size: 399 263 241 63 36 742 85 1071 Cuts at: 4291 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 263 241 85 63 36 BspEI T'CCGG_A Cuts at: 4737 4737 Size: 5074 BspHI T'CATG_A Cuts at: 2963 4693 2963 Size: 1730 3344 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2429 844 Size: 1585 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2961 1394 Size: 429 1138 3507 Fragments arranged by size: 3507 1138 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3569 3743 65 Size: 1183 2321 174 1396 Fragments arranged by size: 2321 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2349 3718 3905 1700 Size: 110 539 1369 187 2869 Fragments arranged by size: 2869 1369 539 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5074 BssHII G'CGCG_C Cuts at: 1925 2319 1925 Size: 394 4680 BssSI C'ACGA_G Cuts at: 3134 4146 3134 Size: 1012 4062 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5074 BstBI TT'CG_AA Cuts at: 3878 3878 Size: 5074 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5074 BstYI r'GATC_y Cuts at: 1972 2404 2771 3156 3173 4625 4711 4723 5069 Size: 432 367 385 17 1452 86 12 346 Cuts at: 5069 1972 Size: 1977 Fragments arranged by size: 1977 1452 432 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2025 2057 2294 513 Size: 552 960 32 237 3293 Fragments arranged by size: 3293 960 552 237 32 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4335 Fragments arranged by size: 4335 683 56 BtsI GCAGTG_nn' Cuts at: 2590 3384 3404 2590 Size: 794 20 4260 Fragments arranged by size: 4260 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5074 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5039 DraI TTT'AAA Cuts at: 2378 2378 Size: 5074 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4081 809 Size: 507 2765 1802 Fragments arranged by size: 2765 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2054 2521 3410 Size: 54 1425 50 275 18 224 467 889 Cuts at: 3410 5039 8 Size: 1629 43 Fragments arranged by size: 1629 1425 889 467 275 224 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2054 2521 1537 Size: 275 242 467 4090 Fragments arranged by size: 4090 467 275 242 EarI CTCTTCn'nnn_ Cuts at: 1353 3004 1353 Size: 1651 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3661 4035 4181 387 Size: 1064 138 2072 374 146 1280 Fragments arranged by size: 2072 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3137 3734 4521 4789 1239 Size: 393 1505 597 787 268 1524 Fragments arranged by size: 1524 1505 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2136 2290 2136 Size: 154 4920 EcoRI G'AATT_C Cuts at: 2383 2761 2383 Size: 378 4696 FspI TGC'GCA Cuts at: 3580 3580 Size: 5074 HaeII r_GCGC'y Cuts at: 1678 2227 2245 4221 1678 Size: 549 18 1976 2531 Fragments arranged by size: 2531 1976 549 18 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2161 2193 3715 3747 3789 Size: 32 135 32 589 32 1522 32 42 Cuts at: 3789 3821 1373 Size: 32 2626 Fragments arranged by size: 2626 1522 589 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2418 2664 669 Size: 988 281 480 246 3079 Fragments arranged by size: 3079 988 480 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5074 HpaI GTT'AAC Cuts at: 2664 2664 Size: 5074 KpnI G_GTAC'C Cuts at: 2399 2399 Size: 5074 MfeI C'AATT_G Cuts at: 2673 2673 Size: 5074 MluI A'CGCG_T Cuts at: 4950 4950 Size: 5074 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4188 4372 1305 Size: 2883 184 2007 Fragments arranged by size: 2883 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3252 Fragments arranged by size: 3252 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 3032 3391 3550 518 Size: 648 1866 359 159 2042 Fragments arranged by size: 2042 1866 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2059 2067 2296 2490 3167 4315 4560 1606 Size: 453 8 229 194 677 1148 245 2120 Fragments arranged by size: 2120 1148 677 453 245 229 194 8 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4522 NdeI CA'TA_TG Cuts at: 387 387 Size: 5074 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5074 NotI GC'GGCC_GC Cuts at: 2521 2521 Size: 5074 NspI r_CATG'y Cuts at: 1132 1229 2331 2432 3977 1132 Size: 97 1102 101 1545 2229 Fragments arranged by size: 2229 1545 1102 101 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5074 PciI A'CATG_T Cuts at: 3973 3973 Size: 5074 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5074 PmeI GTTT'AAAC Cuts at: 2378 2378 Size: 5074 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3143 3175 4656 4688 3143 Size: 32 1481 32 3529 Fragments arranged by size: 3529 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2136 2136 Size: 5074 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5074 PsiI TTA'TAA Cuts at: 2644 2644 Size: 5074 PspOMI G'GGCC_C Cuts at: 2290 2290 Size: 5074 PstI C_TGCA'G Cuts at: 830 2426 830 Size: 1596 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5074 SacII CC_GC'GG Cuts at: 2060 2297 2060 Size: 237 4837 SalI G'TCGA_C Cuts at: 1936 2416 1936 Size: 480 4594 SanDI GG'GwC_CC Cuts at: 2136 2136 Size: 5074 SbfI CC_TGCA'GG Cuts at: 2426 2426 Size: 5074 ScaI AGT'ACT Cuts at: 3322 5034 3322 Size: 1712 3362 SfcI C'TryA_G Cuts at: 826 1045 2314 2422 3557 4238 4429 826 Size: 219 1269 108 1135 681 191 1471 Fragments arranged by size: 1471 1269 1135 681 219 191 108 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5074 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5074 SmaI CCC'GGG Cuts at: 1872 2401 1872 Size: 529 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3178 4079 4341 820 Size: 197 926 1235 901 262 1553 Fragments arranged by size: 1553 1235 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 2865 493 Size: 2372 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5074 SphI G_CATG'C Cuts at: 1132 2331 2432 1132 Size: 1199 101 3774 Fragments arranged by size: 3774 1199 101 SspI AAT'ATT Cuts at: 5 52 2998 5 Size: 47 2946 2081 Fragments arranged by size: 2946 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5074 StyI C'CwwG_G Cuts at: 513 1065 2513 513 Size: 552 1448 3074 Fragments arranged by size: 3074 1448 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3101 3118 3271 3456 3901 325 Size: 1255 1521 17 153 185 445 1498 Fragments arranged by size: 1521 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3320 5032 96 Size: 274 80 33 51 1443 1343 1712 138 Fragments arranged by size: 1712 1443 1343 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5034 5066 5034 Size: 32 5042 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5074 XbaI T'CTAG_A Cuts at: 2410 2410 Size: 5074 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2032 2032 Size: 5074 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5074 XmaI C'CCGG_G Cuts at: 1870 2399 1870 Size: 529 4545 XmnI GAAnn'nnTTC Cuts at: 3203 3203 Size: 5074 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2882 276 Size: 53 83 186 936 1348 2468 Fragments arranged by size: 2468 1348 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspXI PsrI PvuII RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI