Restriction map for
pFN21AB8741
##################################
# NO CUT ENZYMES ( 202 enzymes) #
##################################
AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AflIII AgeI
AhdI Alw44I AlwI AocI Aor51HI
ApaI ApaLI AscI AseI AsnI
Asp700I Asp718I AspEI AspHI AspI
AvaI AviII AvrII BalI BamHI
BanII BbeI BbrPI BbvCI BcgI
BciVI BclI BcnI BfrI BglII
BlnI BlpI BmyI BpmI Bpu1102I
BsaAI BsaBI BsaHI BsaI BsaWI
BseAI BseRI BsgI BsiEI BsiHKAI
BsiWI BsiYI BslI BsmAI BsmBI
BsmI BsoBI Bsp1286I Bsp1407I BspDI
BspEI BspHI BspLU11I BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI
Bst1107I BstBI BstEII BstF5I BstPI
BstUI BstYI BstZ17I Bsu36I CelII
CfoI Cfr10I ClaI CpoI DdeI
DpnII DrdI DsaI EaeI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRV EcoT22I
FauI FbaI FokI FseI FspI
HaeII HapII HgaI HhaI Hin1I
HinP1I HincII HindII HindIII HinfI
HpaI HpaII KasI KpnI Ksp632I
KspI MaeII MamI MboI MfeI
MflI MluI MluNI MroI MscI
MslI MspI MunI MvnI NaeI
NarI NciI NcoI NdeI NdeII
NgoMIV NheI NotI NruI NsiI
NspI NspV PacI PaeR7I PflFI
PflMI PinAI PleI PmaCI PmeI
PmlI PshAI PshBI Psp1406I PstI
PvuI RcaI RsrII SacI SacII
SalI SapI Sau3AI SbfI ScaI
SexAI SfaNI SfcI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
SphI Sse8387I SspBI SspI StuI
SwaI TaqI TfiI Tsp45I Tth111I
TthHB8I Van91I XhoI XhoII XmaCI
XmaI XmnI
###################################
# ONE CUT ENZYMES ( 25 enzymes) #
###################################
AciI 301 CCGC CCGC(-3/-1)
AfaI 409 GTAC GT^AC
AlwNI 155 CAGCAGCTG CAG[ACGT][ACGT][ACGT]^CTG
BanI 283 GGTGCC G^G[CT][GA]CC
BbsI 81 GAAGAC GAAGAC(2/6)
BglI 163 GCCTGGCAGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BpuAI 81 GAAGAC GAAGAC(2/6)
BsrI 338 ACTGG ACTGG(1/-1)
BstAPI 168 GCAGGCCATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
BstXI 331 CCAAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Cac8I 168 GCAGGC GC[ACGT]^[ACGT]GC
DraI 253 TTTAAA TTT^AAA
DraIII 308 CACCAAGTG CAC[ACGT][ACGT][ACGT]^GTG
EcoT14I 86 CCAAGG C^C[AT][AT]GG
HaeIII 171 GGCC GG^CC
HphI 307 TCACC GGTGA(8/7)
MaeIII 246 GTTAC ^GT[ACGT]AC
MspA1I 158 CAGCTG C[AC]G^C[GT]G
PvuII 158 CAGCTG CAG^CTG
RsaI 409 GTAC GT^AC
SmlI 260 CTCAAG C^T[CT][GA]AG
StyI 86 CCAAGG C^C[AT][AT]GG
TspRI 337 CACTG CA[GC]TG[ACGT][ACGT]^
XbaI 40 TCTAGA T^CTAGA
XcmI 328 CCACCAAGCCACTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
###################################
# TWO CUT ENZYMES ( 20 enzymes) #
###################################
AvaII 238 GGTCC G^G[AT]CC
342 GGTCC
BbvI 157 GCAGC GCAGC(8/12)
160 GCTGC
BfaI 41 CTAG C^TAG
227 CTAG
BsaJI 86 CCAAGG C^C[ACGT][ACGT]GG
241 CCCAGG
BsmFI 239 GTCCC GGGAC(10/14)
343 GTCCC
BssKI 164 CCTGG ^CC[ACGT]GG
242 CCAGG
BstNI 164 CCTGG CC^[AT]GG
242 CCAGG
Cfr13I 238 GGTCC G^G[ACGT]CC
342 GGTCC
DraII 237 AGGTCCC [GA]G^G[ACGT]CC[CT]
341 GGGTCCC
EcoO109I 237 AGGTCCC [GA]G^G[ACGT]CC[CT]
341 GGGTCCC
EcoRI 96 GAATTC G^AATTC
222 GAATTC
EcoRII 164 CCTGG ^CC[AT]GG
242 CCAGG
MaeI 41 CTAG C^TAG
227 CTAG
MvaI 164 CCTGG CC^[AT]GG
242 CCAGG
MwoI 163 GCCTGGCAGGC GC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GC
168 GCAGGCCATGC
NlaIII 139 CATG CATG^
174 CATG
PpuMI 237 AGGTCCC [GA]G^G[AT]CC[CT]
341 GGGTCCC
Sau96I 238 GGTCC G^G[ACGT]CC
342 GGTCC
ScrFI 164 CCTGG CC^[ACGT]GG
242 CCAGG
TseI 157 GCAGC G^C[AT]GC
160 GCTGC
#########################################
# 3 OR MORE CUT ENZYMES ( 11 enzymes) #
#########################################
AcsI AluI ApoI Fnu4HI ItaI
MboII MnlI MseI NlaIV Tru9I
Tsp509I