(Circular) (Six-base) MAPSORT of: pFN21AB8740.seq Check: 1310 from: 1 to: 5254 pFN21AB8740 5254 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2463 2463 Size: 5254 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3064 278 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2597 1937 Size: 660 4594 Acc65I G'GTAC_C Cuts at: 2575 2575 Size: 5254 AclI AA'CG_TT Cuts at: 1563 3381 3754 1563 Size: 1818 373 3063 Fragments arranged by size: 3063 1818 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3317 4701 4969 1632 Size: 1685 1384 268 1917 Fragments arranged by size: 1917 1685 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5057 AflIII A'CryG_T Cuts at: 1203 2262 4153 5130 1203 Size: 1059 1891 977 1327 Fragments arranged by size: 1891 1327 1059 977 AgeI A'CCGG_T Cuts at: 4085 4085 Size: 5254 AhdI GACnn_n'nnGTC Cuts at: 3983 3983 Size: 5254 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5254 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5254 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2426 2427 2458 2459 2426 Size: 1 31 1 5221 Fragments arranged by size: 5221 31 1 1 AlwNI CAG_nnn'CTG Cuts at: 2364 2997 4569 2364 Size: 633 1572 3049 Fragments arranged by size: 3049 1572 633 ApaLI G'TGCA_C Cuts at: 3311 4467 3311 Size: 1156 4098 ApoI r'AATT_y Cuts at: 1493 2563 2789 2941 5050 1493 Size: 1070 226 152 2109 1697 Fragments arranged by size: 2109 1697 1070 226 152 AseI AT'TA_AT Cuts at: 160 1032 3808 160 Size: 872 2776 1606 Fragments arranged by size: 2776 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 AvaI C'yCGr_G Cuts at: 1870 1943 2141 2221 2256 2579 1870 Size: 73 198 80 35 323 4545 Fragments arranged by size: 4545 323 198 80 73 35 BamHI G'GATC_C Cuts at: 2584 2951 2584 Size: 367 4887 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2072 2104 2575 4030 618 Size: 325 217 627 285 32 471 1455 1842 Fragments arranged by size: 1842 1455 627 471 325 285 217 32 BanII G_rGCy'C Cuts at: 1391 2573 1391 Size: 1182 4072 BbeI G_GCGC'C Cuts at: 2076 2076 Size: 5254 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2433 928 Size: 971 534 3749 Fragments arranged by size: 3749 971 534 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2227 2261 2287 2321 3445 3479 1903 Size: 34 290 34 26 34 1124 34 3678 Fragments arranged by size: 3678 1124 290 34 34 34 34 26 BciVI GTATCCnnnnn_n' Cuts at: 3146 4356 3146 Size: 1210 4044 BclI T'GATC_A Cuts at: 1548 1548 Size: 5254 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 3865 136 Size: 107 122 71 631 605 2193 1525 Fragments arranged by size: 2193 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5249 5249 Size: 5254 BlpI GC'TnA_GC Cuts at: 2671 2671 Size: 5254 Bme1580I G_kGCm'C Cuts at: 1790 2109 2130 3315 4471 1790 Size: 319 21 1185 1156 2573 Fragments arranged by size: 2573 1185 1156 319 21 BmgBI CAC'GTC Cuts at: 1318 2125 1318 Size: 807 4447 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2204 2985 3943 Size: 416 53 83 399 24 787 781 958 Cuts at: 3943 442 Size: 1753 Fragments arranged by size: 1753 958 787 781 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5254 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2213 3914 1239 Size: 974 1701 2579 Fragments arranged by size: 2579 1701 974 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2270 3379 4244 4542 2270 Size: 1109 865 298 2982 Fragments arranged by size: 2982 1109 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3917 882 Size: 617 2418 2219 Fragments arranged by size: 2418 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2278 3045 493 Size: 711 1074 767 2702 Fragments arranged by size: 2702 1074 767 711 BsaBI GATnn'nnATC Cuts at: 2012 5248 2012 Size: 3236 2018 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2073 2415 3061 3443 Size: 53 83 186 936 540 342 646 382 Cuts at: 3443 4078 275 Size: 635 1451 Fragments arranged by size: 1451 936 646 635 540 382 342 186 83 53 BsaWI w'CCGG_w Cuts at: 3687 4085 4359 4506 4917 3687 Size: 398 274 147 411 4024 Fragments arranged by size: 4024 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5029 Fragments arranged by size: 5029 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2210 2366 1186 Size: 1024 156 4074 Fragments arranged by size: 4074 1024 156 BseYI C'CCAG_C Cuts at: 2108 2342 4457 4953 2108 Size: 234 2115 496 2409 Fragments arranged by size: 2409 2115 496 234 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2306 2450 1163 Size: 573 570 144 3967 Fragments arranged by size: 3967 573 570 144 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2704 3465 4493 1540 Size: 275 186 703 761 1028 2301 Fragments arranged by size: 2301 1028 761 703 275 186 BsiHKAI G_wGCw'C Cuts at: 2478 3315 3400 4471 2478 Size: 837 85 1071 3261 Fragments arranged by size: 3261 1071 837 85 BsmI GAATG_Cn' Cuts at: 2765 2858 2765 Size: 93 5161 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2109 2130 2478 2573 3315 3400 4471 Size: 399 319 21 348 95 742 85 1071 Cuts at: 4471 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 399 348 319 95 85 21 BspEI T'CCGG_A Cuts at: 4917 4917 Size: 5254 BspHI T'CATG_A Cuts at: 3143 4873 3143 Size: 1730 3524 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2463 2609 844 Size: 1619 146 3489 Fragments arranged by size: 3489 1619 146 BsrBI CCG'CTC Cuts at: 1394 1823 3141 1394 Size: 429 1318 3507 Fragments arranged by size: 3507 1318 429 BsrDI GCAATG_nn' Cuts at: 65 1248 3749 3923 65 Size: 1183 2501 174 1396 Fragments arranged by size: 2501 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2338 2417 3898 4085 1700 Size: 110 528 79 1481 187 2869 Fragments arranged by size: 2869 1481 528 187 110 79 BsrGI T'GTAC_A Cuts at: 96 2265 96 Size: 2169 3085 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5254 BssSI C'ACGA_G Cuts at: 3314 4326 3314 Size: 1012 4242 BstAPI GCAn_nnn'nTGC Cuts at: 794 2473 794 Size: 1679 3575 BstBI TT'CG_AA Cuts at: 4058 4058 Size: 5254 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5254 BstYI r'GATC_y Cuts at: 1972 2584 2951 3336 3353 4805 4891 4903 5249 Size: 612 367 385 17 1452 86 12 346 Cuts at: 5249 1972 Size: 1977 Fragments arranged by size: 1977 1452 612 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 513 Size: 552 4702 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 4515 Fragments arranged by size: 4515 683 56 BtsI GCAGTG_nn' Cuts at: 2185 2365 2770 3564 3584 2185 Size: 180 405 794 20 3855 Fragments arranged by size: 3855 794 405 180 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5254 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5219 DraI TTT'AAA Cuts at: 2558 2558 Size: 5254 DraIII CAC_nnn'GTG Cuts at: 2278 2473 2278 Size: 195 5059 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4261 809 Size: 507 2945 1802 Fragments arranged by size: 2945 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2701 3590 5219 Size: 54 1425 50 275 18 871 889 1629 Cuts at: 5219 8 Size: 43 Fragments arranged by size: 1629 1425 889 871 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2701 1537 Size: 275 889 4090 Fragments arranged by size: 4090 889 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3184 1353 Size: 1831 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 3841 4215 4361 387 Size: 1064 138 2252 374 146 1280 Fragments arranged by size: 2252 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2213 3317 3914 4701 4969 1239 Size: 393 581 1104 597 787 268 1524 Fragments arranged by size: 1524 1104 787 597 581 393 268 EcoO109I rG'GnC_Cy Cuts at: 2317 2317 Size: 5254 EcoRI G'AATT_C Cuts at: 2563 2941 2563 Size: 378 4876 FspI TGC'GCA Cuts at: 3760 3760 Size: 5254 HaeII r_GCGC'y Cuts at: 1678 2076 2232 4401 1678 Size: 398 156 2169 2531 Fragments arranged by size: 2531 2169 398 156 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3895 3927 3969 4001 1373 Size: 32 135 32 2323 32 42 32 2626 Fragments arranged by size: 2626 2323 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2598 2844 669 Size: 988 281 660 246 3079 Fragments arranged by size: 3079 988 660 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5254 HpaI GTT'AAC Cuts at: 2844 2844 Size: 5254 KasI G'GCGC_C Cuts at: 2072 2072 Size: 5254 KpnI G_GTAC'C Cuts at: 2579 2579 Size: 5254 MfeI C'AATT_G Cuts at: 2542 2853 2542 Size: 311 4943 MluI A'CGCG_T Cuts at: 5130 5130 Size: 5254 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 4368 4552 1305 Size: 3063 184 2007 Fragments arranged by size: 3063 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3432 Fragments arranged by size: 3432 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2384 3212 3571 3730 518 Size: 648 1218 828 359 159 2042 Fragments arranged by size: 2042 1218 828 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2670 3347 4495 4740 1606 Size: 1064 677 1148 245 2120 Fragments arranged by size: 2120 1148 1064 677 245 NaeI GCC'GGC Cuts at: 2419 2419 Size: 5254 NarI GG'CG_CC Cuts at: 2073 2073 Size: 5254 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 4702 NdeI CA'TA_TG Cuts at: 387 387 Size: 5254 NgoMIV G'CCGG_C Cuts at: 2417 2417 Size: 5254 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5254 NotI GC'GGCC_GC Cuts at: 2701 2701 Size: 5254 NspI r_CATG'y Cuts at: 1132 1229 2266 2612 4157 1132 Size: 97 1037 346 1545 2229 Fragments arranged by size: 2229 1545 1037 346 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5254 PciI A'CATG_T Cuts at: 2262 4153 2262 Size: 1891 3363 PflMI CCAn_nnn'nTGG Cuts at: 1108 2342 1108 Size: 1234 4020 PmeI GTTT'AAAC Cuts at: 2558 2558 Size: 5254 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3323 3355 4836 4868 3323 Size: 32 1481 32 3709 Fragments arranged by size: 3709 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2317 2317 Size: 5254 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5254 PsiI TTA'TAA Cuts at: 2824 2824 Size: 5254 PstI C_TGCA'G Cuts at: 830 2606 830 Size: 1776 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5254 SalI G'TCGA_C Cuts at: 1936 2596 1936 Size: 660 4594 SbfI CC_TGCA'GG Cuts at: 2606 2606 Size: 5254 ScaI AGT'ACT Cuts at: 3502 5214 3502 Size: 1712 3542 SfcI C'TryA_G Cuts at: 826 1045 2602 3737 4418 4609 826 Size: 219 1557 1135 681 191 1471 Fragments arranged by size: 1557 1471 1135 681 219 191 SfoI GGC'GCC Cuts at: 2074 2074 Size: 5254 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5254 SgrAI Cr'CCGG_yG Cuts at: 1700 2417 1700 Size: 717 4537 SmaI CCC'GGG Cuts at: 1872 2143 2581 1872 Size: 271 438 4545 Fragments arranged by size: 4545 438 271 SmlI C'TyrA_G Cuts at: 820 1017 1943 2285 3358 4259 4521 820 Size: 197 926 342 1073 901 262 1553 Fragments arranged by size: 1553 1073 926 901 342 262 197 SnaBI TAC'GTA Cuts at: 493 3045 493 Size: 2552 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5254 SphI G_CATG'C Cuts at: 1132 2612 1132 Size: 1480 3774 SspI AAT'ATT Cuts at: 5 52 3178 5 Size: 47 3126 2081 Fragments arranged by size: 3126 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5254 StyI C'CwwG_G Cuts at: 513 1065 2373 2693 513 Size: 552 1308 320 3074 Fragments arranged by size: 3074 1308 552 320 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3281 3298 3451 3636 4081 325 Size: 1255 1701 17 153 185 445 1498 Fragments arranged by size: 1701 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2265 3500 5212 Size: 274 80 33 51 1443 288 1235 1712 Cuts at: 5212 96 Size: 138 Fragments arranged by size: 1712 1443 1235 288 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5214 5246 5214 Size: 32 5222 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5254 XbaI T'CTAG_A Cuts at: 2590 2590 Size: 5254 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2380 2380 Size: 5254 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5254 XmaI C'CCGG_G Cuts at: 1870 2141 2579 1870 Size: 271 438 4545 Fragments arranged by size: 4545 438 271 XmnI GAAnn'nnTTC Cuts at: 2063 3383 2063 Size: 1320 3934 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3062 276 Size: 53 83 186 936 1528 2468 Fragments arranged by size: 2468 1528 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI AvrII BaeI BbvCI BfrBI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FalI FseI FspAI NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SrfI SwaI