Restriction map for
pFN21AB8734
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# NO CUT ENZYMES ( 138 enzymes) #
##################################
AatII Acc65I AccI AcsI AcyI
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI ApoI AscI
Asp718I AspEI AspI AvaI AviII
BalI BanII BbeI BbrPI BbvCI
BcgI BciVI BclI BcnI BglI
BlpI Bpu1102I BsaAI BsaHI BsaI
BsiEI BsmBI BsoBI Bsp1407I BspDI
BspHI BspLU11I BsrBI BsrDI BsrFI
BsrGI BssHII Bst1107I BstAPI BstBI
BstEII BstPI BstXI BstZ17I Bsu36I
CelII Cfr10I ClaI DrdI EaeI
EagI Eam1105I EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRV
EcoT22I FauI FbaI FseI FspI
HaeII HgaI Hin1I HincII HindII
HpaI KasI KpnI KspI MluI
MluNI MscI NaeI NarI NciI
NcoI NdeI NgoMIV NotI NruI
NsiI NspI NspV PacI PaeR7I
PflFI PflMI PinAI PmaCI PmeI
PmlI PshAI PvuI PvuII RcaI
SacI SacII SalI SapI SbfI
ScaI SexAI SfiI SfoI SfuI
SgrAI SmaI SnaBI SpeI SphI
Sse8387I SspBI SspI SwaI TaqI
Tth111I TthHB8I Van91I XbaI XcmI
XhoI XmaCI XmaI
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# ONE CUT ENZYMES ( 49 enzymes) #
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AccIII 114 TCCGGA T^CCGGA
AclI 999 AACGTT AA^CGTT
AflII 1287 CTTAAG C^TTAAG
AlwNI 812 CAGATTCTG CAG[ACGT][ACGT][ACGT]^CTG
AseI 543 ATTAAT AT^TAAT
AsnI 543 ATTAAT AT^TAAT
Asp700I 169 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
BamHI 1026 GGATCC G^GATCC
BanI 41 GGCACC G^G[CT][GA]CC
BbsI 154 GTCTTC GAAGAC(2/6)
BfrI 1287 CTTAAG C^TTAAG
BglII 1173 AGATCT A^GATCT
BpuAI 154 GTCTTC GAAGAC(2/6)
BsaBI 1579 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BsaWI 114 TCCGGA [AT]^CCGG[AT]
BseAI 114 TCCGGA T^CCGGA
BsgI 19 GTGCAG GTGCAG(16/14)
BsiWI 105 CGTACG C^GTACG
BsmAI 976 GAGAC GTCTC(1/5)
BsmFI 419 GGGAC GGGAC(10/14)
BsmI 1179 GCATTC GAATGC(1/-1)
BspEI 114 TCCGGA T^CCGGA
BspMI 21 GCAGGT ACCTGC(4/8)
BssSI 9 CTCGTG CACGAG(-5/-1)
CpoI 111 CGGTCCG CG^G[AT]CCG
DraIII 136 CACTTTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 71 CCGTGG C^C[GA][CT]GG
EarI 679 GAAGAG CTCTTC(1/4)
HapII 115 CCGG C^CGG
HindIII 171 AAGCTT A^AGCTT
HpaII 115 CCGG C^CGG
Ksp632I 679 GAAGAG CTCTTC(1/4)
MaeII 1000 ACGT A^CGT
MamI 1579 GATTTTGATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MfeI 251 CAATTG C^AATTG
MroI 114 TCCGGA T^CCGGA
MspA1I 351 CCGCTG C[AC]G^C[GT]G
MspI 115 CCGG C^CGG
MunI 251 CAATTG C^AATTG
NheI 4 GCTAGC G^CTAGC
PshBI 543 ATTAAT AT^TAAT
Psp1406I 999 AACGTT AA^CGTT
PstI 1516 CTGCAG CTGCA^G
RsrII 111 CGGTCCG CG^G[AT]CCG
SfaNI 318 GCATC GCATC(5/9)
SmlI 1287 CTTAAG C^T[CT][GA]AG
StuI 1246 AGGCCT AGG^CCT
Tsp45I 1445 GTGAC ^GT[GC]AC
XmnI 169 GAAAGCTTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
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# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 46 CGCG CG^CG
109 CGCG
AfaI 106 GTAC GT^AC
518 GTAC
AspHI 118 GAGCAC G[AT]GC[AT]^C
553 GAGCAC
AvrII 178 CCTAGG C^CTAGG
283 CCTAGG
BlnI 178 CCTAGG C^CTAGG
283 CCTAGG
BsiHKAI 118 GAGCAC G[AT]GC[AT]^C
553 GAGCAC
BstUI 46 CGCG CG^CG
109 CGCG
CfoI 47 GCGC GCG^C
884 GCGC
DraI 265 TTTAAA TTT^AAA
868 TTTAAA
DraII 419 GGGACCC [GA]G^G[ACGT]CC[CT]
1149 AGGTCCT
EcoO109I 419 GGGACCC [GA]G^G[ACGT]CC[CT]
1149 AGGTCCT
EcoT14I 178 CCTAGG C^C[AT][AT]GG
283 CCTAGG
HhaI 47 GCGC GCG^C
884 GCGC
HinP1I 47 GCGC G^CGC
884 GCGC
MslI 589 CATAAAAGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1180 CATTCTGATG
MvnI 46 CGCG CG^CG
109 CGCG
NlaIII 723 CATG CATG^
943 CATG
PleI 577 GACTC GAGTC(4/5)
1231 GACTC
PpuMI 419 GGGACCC [GA]G^G[AT]CC[CT]
1149 AGGTCCT
RsaI 106 GTAC GT^AC
518 GTAC
SfcI 1516 CTGCAG C^T[GA][CT]AG
1621 CTACAG
StyI 178 CCTAGG C^C[AT][AT]GG
283 CCTAGG
#########################################
# 3 OR MORE CUT ENZYMES ( 49 enzymes) #
#########################################
AciI AluI AlwI AvaII BbvI
BfaI BmyI BpmI BsaJI BseRI
BsiYI BslI Bsp1286I BsrI BssKI
BstF5I BstNI BstYI Cac8I Cfr13I
DdeI DpnII EcoRII Fnu4HI FokI
HaeIII HinfI HphI ItaI MaeI
MaeIII MboI MboII MflI MnlI
MseI MvaI MwoI NdeII NlaIV
Sau3AI Sau96I ScrFI TfiI Tru9I
TseI Tsp509I TspRI XhoII