(Circular) (Six-base) MAPSORT of: pFN21AB8734.seq Check: 5478 from: 1 to: 6358 pFN21AB8734 6358 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2017 2017 Size: 6358 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4168 278 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3701 1937 Size: 1764 4594 Acc65I G'GTAC_C Cuts at: 3679 3679 Size: 6358 AclI AA'CG_TT Cuts at: 1563 3005 4485 4858 1563 Size: 1442 1480 373 3063 Fragments arranged by size: 3063 1480 1442 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2432 2844 4421 5805 6073 1632 Size: 800 412 1577 1384 268 1917 Fragments arranged by size: 1917 1577 1384 800 412 268 AflII C'TTAA_G Cuts at: 820 1017 3292 820 Size: 197 2275 3886 Fragments arranged by size: 3886 2275 197 AflIII A'CryG_T Cuts at: 1203 5257 6234 1203 Size: 4054 977 1327 Fragments arranged by size: 4054 1327 977 AgeI A'CCGG_T Cuts at: 5189 5189 Size: 6358 AhdI GACnn_n'nnGTC Cuts at: 5087 5087 Size: 6358 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6358 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6358 AlwNI CAG_nnn'CTG Cuts at: 2822 4101 5673 2822 Size: 1279 1572 3507 Fragments arranged by size: 3507 1572 1279 ApaLI G'TGCA_C Cuts at: 4415 5571 4415 Size: 1156 5202 ApoI r'AATT_y Cuts at: 1493 3667 3893 4045 6154 1493 Size: 2174 226 152 2109 1697 Fragments arranged by size: 2174 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2549 4912 160 Size: 872 1517 2363 1606 Fragments arranged by size: 2363 1606 1517 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 AvaI C'yCGr_G Cuts at: 1870 1943 3683 1870 Size: 73 1740 4545 Fragments arranged by size: 4545 1740 73 AvrII C'CTAG_G Cuts at: 2183 2288 2183 Size: 105 6253 BamHI G'GATC_C Cuts at: 3031 3688 4055 3031 Size: 657 367 5334 Fragments arranged by size: 5334 657 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2046 3679 5134 618 Size: 325 217 627 259 1633 1455 1842 Fragments arranged by size: 1842 1633 1455 627 325 259 217 BanII G_rGCy'C Cuts at: 1391 3677 1391 Size: 2286 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2152 928 Size: 971 253 5134 Fragments arranged by size: 5134 971 253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4549 4583 1903 Size: 34 2612 34 3678 Fragments arranged by size: 3678 2612 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4250 5460 4250 Size: 1210 5148 BclI T'GATC_A Cuts at: 1548 1548 Size: 6358 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4969 136 Size: 107 122 71 631 605 3297 1525 Fragments arranged by size: 3297 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 3178 6353 3178 Size: 3175 3183 BlpI GC'TnA_GC Cuts at: 3775 3775 Size: 6358 Bme1580I G_kGCm'C Cuts at: 1790 2049 4419 5575 1790 Size: 259 2370 1156 2573 Fragments arranged by size: 2573 2370 1156 259 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6358 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3084 4089 5047 Size: 416 53 83 399 24 1667 1005 958 Cuts at: 5047 442 Size: 1753 Fragments arranged by size: 1753 1667 1005 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2013 1056 Size: 957 5401 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2057 2569 3088 3094 3100 3106 5018 1239 Size: 818 512 519 6 6 6 1912 2579 Fragments arranged by size: 2579 1912 818 519 512 6 6 6 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4483 5348 5646 4483 Size: 865 298 5195 Fragments arranged by size: 5195 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 5021 882 Size: 617 3522 2219 Fragments arranged by size: 3522 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 4149 493 Size: 711 2945 2702 Fragments arranged by size: 2945 2702 711 BsaBI GATnn'nnATC Cuts at: 3588 6352 3588 Size: 2764 3594 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4165 4547 5182 275 Size: 53 83 186 936 2632 382 635 1451 Fragments arranged by size: 2632 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2119 4791 5189 5463 5610 6021 2119 Size: 2672 398 274 147 411 2456 Fragments arranged by size: 2672 2456 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6133 Fragments arranged by size: 6133 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3068 3215 3279 1186 Size: 1882 147 64 4265 Fragments arranged by size: 4265 1882 147 64 BseYI C'CCAG_C Cuts at: 2041 5561 6057 2041 Size: 3520 496 2342 Fragments arranged by size: 3520 2342 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2045 1163 Size: 573 309 5476 Fragments arranged by size: 5476 573 309 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3808 4569 5597 1540 Size: 275 186 1807 761 1028 2301 Fragments arranged by size: 2301 1807 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2127 2562 4419 4504 5575 2127 Size: 435 1857 85 1071 2910 Fragments arranged by size: 2910 1857 1071 435 85 BsiWI C'GTAC_G Cuts at: 2110 2110 Size: 6358 BsmI GAATG_Cn' Cuts at: 3184 3869 3962 3184 Size: 685 93 5580 Fragments arranged by size: 5580 685 93 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2049 2127 2562 3677 4419 4504 5575 Size: 399 259 78 435 1115 742 85 1071 Cuts at: 5575 1391 Size: 2174 Fragments arranged by size: 2174 1115 1071 742 435 399 259 85 78 BspEI T'CCGG_A Cuts at: 2119 6021 2119 Size: 3902 2456 BspHI T'CATG_A Cuts at: 4247 5977 4247 Size: 1730 4628 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2017 3713 844 Size: 1173 1696 3489 Fragments arranged by size: 3489 1696 1173 BsrBI CCG'CTC Cuts at: 1394 1823 4245 1394 Size: 429 2422 3507 Fragments arranged by size: 3507 2422 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4853 5027 65 Size: 1183 3605 174 1396 Fragments arranged by size: 3605 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 5002 5189 1700 Size: 110 3192 187 2869 Fragments arranged by size: 3192 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6358 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6358 BssSI C'ACGA_G Cuts at: 2014 4418 5430 2014 Size: 2404 1012 2942 Fragments arranged by size: 2942 2404 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6358 BstBI TT'CG_AA Cuts at: 5162 5162 Size: 6358 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6358 BstYI r'GATC_y Cuts at: 1972 2206 3031 3178 3688 4055 4440 4457 5909 Size: 234 825 147 510 367 385 17 1452 Cuts at: 5909 5995 6007 6353 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 825 510 385 367 346 234 147 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2076 513 Size: 552 939 72 4795 Fragments arranged by size: 4795 939 552 72 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5619 Fragments arranged by size: 5619 683 56 BtsI GCAGTG_nn' Cuts at: 3874 4668 4688 3874 Size: 794 20 5544 Fragments arranged by size: 5544 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6358 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6323 DraI TTT'AAA Cuts at: 2272 2875 3662 2272 Size: 603 787 4968 Fragments arranged by size: 4968 787 603 DraIII CAC_nnn'GTG Cuts at: 2146 2146 Size: 6358 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5365 809 Size: 507 4049 1802 Fragments arranged by size: 4049 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3805 4694 6323 Size: 54 1425 50 275 18 1975 889 1629 Cuts at: 6323 8 Size: 43 Fragments arranged by size: 1975 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3805 1537 Size: 275 1993 4090 Fragments arranged by size: 4090 1993 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2679 4288 1353 Size: 1326 1609 3423 Fragments arranged by size: 3423 1609 1326 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4945 5319 5465 387 Size: 1064 138 3356 374 146 1280 Fragments arranged by size: 3356 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2057 2432 2569 2844 3088 3094 3100 Size: 393 425 375 137 275 244 6 6 Cuts at: 3100 3106 4421 5018 5805 6073 1239 Size: 6 1315 597 787 268 1524 Fragments arranged by size: 1524 1315 787 597 425 393 375 275 268 244 137 6 6 6 EcoO109I rG'GnC_Cy Cuts at: 2425 3155 2425 Size: 730 5628 EcoRI G'AATT_C Cuts at: 3667 4045 3667 Size: 378 5980 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2725 2725 Size: 6358 FspI TGC'GCA Cuts at: 4864 4864 Size: 6358 HaeII r_GCGC'y Cuts at: 1678 5505 1678 Size: 3827 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3047 3079 3191 3221 3223 Size: 32 135 32 1475 32 112 30 2 Cuts at: 3223 3253 3581 3613 4999 5031 5073 5105 1373 Size: 30 328 32 1386 32 42 32 2626 Fragments arranged by size: 2626 1475 1386 328 135 112 42 32 32 32 32 32 32 30 30 2 HincII GTy'rAC Cuts at: 669 1657 1938 3702 3948 669 Size: 988 281 1764 246 3079 Fragments arranged by size: 3079 1764 988 281 246 HindIII A'AGCT_T Cuts at: 748 2176 748 Size: 1428 4930 HpaI GTT'AAC Cuts at: 3948 3948 Size: 6358 KpnI G_GTAC'C Cuts at: 3683 3683 Size: 6358 MfeI C'AATT_G Cuts at: 2256 3957 2256 Size: 1701 4657 MluI A'CGCG_T Cuts at: 6234 6234 Size: 6358 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5472 5656 1305 Size: 4167 184 2007 Fragments arranged by size: 4167 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4536 Fragments arranged by size: 4536 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2598 3189 4316 4675 4834 518 Size: 648 1432 591 1127 359 159 2042 Fragments arranged by size: 2042 1432 1127 648 591 359 159 MspA1I CmG'CkG Cuts at: 1606 2358 3774 4451 5599 5844 1606 Size: 752 1416 677 1148 245 2120 Fragments arranged by size: 2120 1416 1148 752 677 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4867 Fragments arranged by size: 4867 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6358 NheI G'CTAG_C Cuts at: 1052 2009 1052 Size: 957 5401 NotI GC'GGCC_GC Cuts at: 3805 3805 Size: 6358 NspI r_CATG'y Cuts at: 1132 1229 3716 5261 1132 Size: 97 2487 1545 2229 Fragments arranged by size: 2487 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6358 PciI A'CATG_T Cuts at: 5257 5257 Size: 6358 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6358 PmeI GTTT'AAAC Cuts at: 3662 3662 Size: 6358 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4427 4459 5940 5972 4427 Size: 32 1481 32 4813 Fragments arranged by size: 4813 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2425 3155 2425 Size: 730 5628 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6358 PsiI TTA'TAA Cuts at: 3928 3928 Size: 6358 PstI C_TGCA'G Cuts at: 830 3525 3710 830 Size: 2695 185 3478 Fragments arranged by size: 3478 2695 185 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6358 RsrII CG'GwC_CG Cuts at: 2117 2117 Size: 6358 SalI G'TCGA_C Cuts at: 1936 3700 1936 Size: 1764 4594 SanDI GG'GwC_CC Cuts at: 2425 2425 Size: 6358 SbfI CC_TGCA'GG Cuts at: 3710 3710 Size: 6358 ScaI AGT'ACT Cuts at: 4606 6318 4606 Size: 1712 4646 SfcI C'TryA_G Cuts at: 826 1045 3521 3626 3706 4841 5522 5713 826 Size: 219 2476 105 80 1135 681 191 1471 Fragments arranged by size: 2476 1471 1135 681 219 191 105 80 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6358 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6358 SmaI CCC'GGG Cuts at: 1872 3685 1872 Size: 1813 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3292 4462 5363 5625 820 Size: 197 926 1349 1170 901 262 1553 Fragments arranged by size: 1553 1349 1170 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4149 493 Size: 3656 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6358 SphI G_CATG'C Cuts at: 1132 3716 1132 Size: 2584 3774 SspI AAT'ATT Cuts at: 5 52 4282 5 Size: 47 4230 2081 Fragments arranged by size: 4230 2081 47 StuI AGG'CCT Cuts at: 1517 3253 1517 Size: 1736 4622 StyI C'CwwG_G Cuts at: 513 1065 2004 2183 2288 3797 513 Size: 552 939 179 105 1509 3074 Fragments arranged by size: 3074 1509 939 552 179 105 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2105 4385 4402 4555 4740 5185 325 Size: 1255 525 2280 17 153 185 445 1498 Fragments arranged by size: 2280 1498 1255 525 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4604 6316 96 Size: 274 80 33 51 1443 2627 1712 138 Fragments arranged by size: 2627 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6318 6350 6318 Size: 32 6326 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6358 XbaI T'CTAG_A Cuts at: 3694 3694 Size: 6358 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6358 XmaI C'CCGG_G Cuts at: 1870 3683 1870 Size: 1813 4545 XmnI GAAnn'nnTTC Cuts at: 2178 4487 2178 Size: 2309 4049 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4166 276 Size: 53 83 186 936 2632 2468 Fragments arranged by size: 2632 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsiWI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI RsrII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI BplI Bpu10I BsmBI BstXI BstZ17I Bsu36I EcoICRI EcoNI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PspXI PsrI PvuII SacI SacII SapI SexAI SfiI SfoI SrfI SwaI XcmI