Restriction map for
pFN21AB8734
(Linear) (Six-base) MAPSORT of: KIBB8734.seq Check: 8463 from: 1 to: 1653
KIBB8734.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 12 1653
Size: 12 1641
AclI AA'CG_TT
Cuts at: 0 1000 1653
Size: 1000 653
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 427 839 1653
Size: 427 412 814
Fragments arranged by size:
814 427 412
AflII C'TTAA_G
Cuts at: 0 1287 1653
Size: 1287 366
AlwNI CAG_nnn'CTG
Cuts at: 0 817 1653
Size: 817 836
AseI AT'TA_AT
Cuts at: 0 544 1653
Size: 544 1109
AvrII C'CTAG_G
Cuts at: 0 178 283 1653
Size: 178 105 1370
Fragments arranged by size:
1370 178 105
BamHI G'GATC_C
Cuts at: 0 1026 1653
Size: 1026 627
BanI G'GyrC_C
Cuts at: 0 41 1653
Size: 41 1612
BbsI GAAGACnn'nnnn_
Cuts at: 0 147 1653
Size: 147 1506
BglII A'GATC_T
Cuts at: 0 1173 1653
Size: 1173 480
Bme1580I G_kGCm'C
Cuts at: 0 44 1653
Size: 44 1609
BmrI ACTGGGnnnn_n'
Cuts at: 0 1079 1653
Size: 1079 574
BmtI G_CTAG'C
Cuts at: 0 8 1653
Size: 8 1645
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 564 1083 1089 1095 1101 1653
Size: 52 512 519 6 6 6 552
Fragments arranged by size:
552 519 512 52 6 6 6
BsaBI GATnn'nnATC
Cuts at: 0 1583 1653
Size: 1583 70
BsaWI w'CCGG_w
Cuts at: 0 114 1653
Size: 114 1539
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1063 1210 1274 1653
Size: 1063 147 64 379
Fragments arranged by size:
1063 379 147 64
BseYI C'CCAG_C
Cuts at: 0 36 1653
Size: 36 1617
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 40 1653
Size: 40 1613
BsiHKAI G_wGCw'C
Cuts at: 0 122 557 1653
Size: 122 435 1096
Fragments arranged by size:
1096 435 122
BsiWI C'GTAC_G
Cuts at: 0 105 1653
Size: 105 1548
BsmI GAATG_Cn'
Cuts at: 0 1179 1653
Size: 1179 474
Bsp1286I G_dGCh'C
Cuts at: 0 44 122 557 1653
Size: 44 78 435 1096
Fragments arranged by size:
1096 435 78 44
BspEI T'CCGG_A
Cuts at: 0 114 1653
Size: 114 1539
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 12 1653
Size: 12 1641
BssSI C'ACGA_G
Cuts at: 0 9 1653
Size: 9 1644
BstYI r'GATC_y
Cuts at: 0 201 1026 1173 1653
Size: 201 825 147 480
Fragments arranged by size:
825 480 201 147
BtgI C'CryG_G
Cuts at: 0 71 1653
Size: 71 1582
DraI TTT'AAA
Cuts at: 0 267 870 1653
Size: 267 603 783
Fragments arranged by size:
783 603 267
DraIII CAC_nnn'GTG
Cuts at: 0 141 1653
Size: 141 1512
EarI CTCTTCn'nnn_
Cuts at: 0 674 1653
Size: 674 979
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 52 427 564 839 1083 1089 1095 1101
Size: 52 375 137 275 244 6 6 6
Cuts at: 1101 1653
Size: 552
Fragments arranged by size:
552 375 275 244 137 52 6 6
6
EcoO109I rG'GnC_Cy
Cuts at: 0 420 1150 1653
Size: 420 730 503
Fragments arranged by size:
730 503 420
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 720 1653
Size: 720 933
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1042 1074 1186 1216 1218 1248 1576 1608
Size: 1042 32 112 30 2 30 328 32
Cuts at: 1608 1653
Size: 45
Fragments arranged by size:
1042 328 112 45 32 32 30 30
2
HindIII A'AGCT_T
Cuts at: 0 171 1653
Size: 171 1482
MfeI C'AATT_G
Cuts at: 0 251 1653
Size: 251 1402
MslI CAynn'nnrTG
Cuts at: 0 593 1184 1653
Size: 593 591 469
Fragments arranged by size:
593 591 469
MspA1I CmG'CkG
Cuts at: 0 353 1653
Size: 353 1300
NheI G'CTAG_C
Cuts at: 0 4 1653
Size: 4 1649
PpuMI rG'GwC_Cy
Cuts at: 0 420 1150 1653
Size: 420 730 503
Fragments arranged by size:
730 503 420
PstI C_TGCA'G
Cuts at: 0 1520 1653
Size: 1520 133
RsrII CG'GwC_CG
Cuts at: 0 112 1653
Size: 112 1541
SanDI GG'GwC_CC
Cuts at: 0 420 1653
Size: 420 1233
SfcI C'TryA_G
Cuts at: 0 1516 1621 1653
Size: 1516 105 32
Fragments arranged by size:
1516 105 32
SmlI C'TyrA_G
Cuts at: 0 1287 1653
Size: 1287 366
StuI AGG'CCT
Cuts at: 0 1248 1653
Size: 1248 405
StyI C'CwwG_G
Cuts at: 0 178 283 1653
Size: 178 105 1370
Fragments arranged by size:
1370 178 105
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 100 1653
Size: 100 1553
XmnI GAAnn'nnTTC
Cuts at: 0 173 1653
Size: 173 1480
Enzymes that do cut:
AarI AclI AcuI AflII AlwNI AseI AvrII BamHI
BanI BbsI BglII Bme1580I BmrI BmtI BpmI BsaBI
BsaWI BseRI BseYI BsgI BsiHKAI BsiWI BsmI Bsp1286I
BspEI BspMI BssSI BstYI BtgI DraI DraIII EarI
Eco57MI EcoO109I FalI Hin4I HindIII MfeI MslI MspA1I
NheI PpuMI PstI RsrII SanDI SfcI SmlI StuI
StyI TaqII XmnI
Enzymes that do not cut:
AatII AccI Acc65I AfeI AflIII AgeI AhdI AleI
AlfI AloI ApaI ApaLI ApoI AscI AsiSI AvaI
BaeI BanII BbeI BbvCI BcgI BciVI BclI BfrBI
BglI BlpI BmgBI BplI Bpu10I BpuEI BsaI BsaAI
BsaHI BsaXI BsiEI BsmBI BspHI BsrBI BsrDI BsrFI
BsrGI BssHII BstAPI BstBI BstEII BstXI BstZ17I Bsu36I
BtgZI BtsI ClaI CspCI DrdI EaeI EagI EciI
EcoICRI EcoNI EcoRI EcoRV FseI FspI FspAI HaeII
HincII HpaI KasI KpnI MluI MmeI MscI NaeI
NarI NcoI NdeI NgoMIV NotI NruI NsiI NspI
PacI PasI PciI PflMI PfoI PmeI PmlI PpiI
PshAI PsiI PspOMI PspXI PsrI PvuI PvuII SacI
SacII SalI SapI SbfI ScaI SexAI SfiI SfoI
SgfI SgrAI SmaI SnaBI SpeI SphI SrfI SspI
SwaI TatI TstI Tth111I XbaI XcmI XhoI XmaI
ZraI