Restriction map for
pFN21AB8732
##################################
# NO CUT ENZYMES ( 117 enzymes) #
##################################
AatII Acc65I AccIII AclI AflII
AflIII AgeI AhdI Alw44I AocI
Aor51HI ApaI ApaLI AscI Asp700I
Asp718I AspEI AspHI AspI AvaI
AviII BalI BbeI BbrPI BbsI
BcgI BciVI BfrI BglII BpuAI
BsaAI BsaBI BsaWI BseAI BsgI
BsiHKAI BsiWI BsmBI BsmI BsoBI
Bsp1407I BspDI BspEI BspHI BspLU11I
BsrBI BsrGI BssHII Bst1107I BstEII
BstPI BstXI BstZ17I Bsu36I ClaI
CpoI DraI DraIII Eam1105I Eco47III
Eco81I EcoO65I EcoRV FseI FspI
HindIII HpaI KasI KpnI MaeII
MamI MluI MluNI MroI MscI
NaeI NarI NgoMIV NheI NotI
NruI PacI PaeR7I PflFI PinAI
PmaCI PmeI PmlI PshAI Psp1406I
PvuI RcaI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI
SgrAI SmaI SnaBI SpeI Sse8387I
SspBI SspI StuI SwaI TfiI
Tth111I XbaI XcmI XhoI XmaCI
XmaI XmnI
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# ONE CUT ENZYMES ( 43 enzymes) #
###################################
AccI 1044 GTCGAC GT^[AC][GT]AC
AcyI 1041 GGCGTC G[GA]^CG[CT]C
AseI 1501 ATTAAT AT^TAAT
AsnI 1501 ATTAAT AT^TAAT
BamHI 444 GGATCC G^GATCC
BanI 574 GGTGCC G^G[CT][GA]CC
BclI 455 TGATCA T^GATCA
BcnI 29 CCGGG CC^[GC]GG
BglI 952 GCCCTGGCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BsaHI 1041 GGCGTC G[GA]^CG[CT]C
BsaI 852 GGTCTC GGTCTC(1/5)
BsiEI 26 CGGCCG CG[GA][CT]^CG
BsrFI 437 ACCGGC [GA]^CCGG[CT]
BssSI 364 CTCGTG CACGAG(-5/-1)
BstBI 190 TTCGAA TT^CGAA
Cfr10I 437 ACCGGC [GA]^CCGG[CT]
DrdI 1035 GACCCTGGCGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
EaeI 26 CGGCCG [CT]^GGCC[GA]
EagI 26 CGGCCG C^GGCCG
EarI 652 GAAGAG CTCTTC(1/4)
EclXI 26 CGGCCG C^GGCCG
Eco52I 26 CGGCCG C^GGCCG
EcoNI 229 CCTGCCCTAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT22I 1405 ATGCAT ATGCA^T
FauI 19 CCCGC CCCGC(4/6)
FbaI 455 TGATCA T^GATCA
HgaI 1042 GCGTC GACGC(5/10)
Hin1I 1041 GGCGTC G[GA]^CG[CT]C
HincII 1044 GTCGAC GT[CT]^[GA]AC
HindII 1044 GTCGAC GT[CT]^[GA]AC
Ksp632I 652 GAAGAG CTCTTC(1/4)
KspI 20 CCGCGG CCGC^GG
MfeI 1330 CAATTG C^AATTG
MunI 1330 CAATTG C^AATTG
NciI 29 CCGGG CC^[GC]GG
NsiI 1405 ATGCAT ATGCA^T
NspV 190 TTCGAA TT^CGAA
PpuMI 32 GGGACCC [GA]G^G[AT]CC[CT]
PshBI 1501 ATTAAT AT^TAAT
SacII 20 CCGCGG CCGC^GG
SalI 1044 GTCGAC G^TCGAC
SfuI 190 TTCGAA TT^CGAA
SphI 239 GCATGC GCATG^C
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AvrII 234 CCTAGG C^CTAGG
1138 CCTAGG
BanII 1235 GGGCTC G[GA]GC[CT]^C
1644 GAGCCC
BbvCI 270 CCTCAGC CCTCAGC(-5/-2)
118 GCTGAGG
BfaI 235 CTAG C^TAG
1139 CTAG
BlnI 234 CCTAGG C^CTAGG
1138 CCTAGG
BlpI 543 GCTCAGC GC^T[ACGT]AGC
1274 GCTCAGC
BmyI 1235 GGGCTC G[AGT]GC[ACT]^C
1644 GAGCCC
Bpu1102I 543 GCTCAGC GC^T[ACGT]AGC
1274 GCTCAGC
BseRI 212 CTCCTC GAGGAG(10/8)
1586 CTCCTC
Bsp1286I 1235 GGGCTC G[AGT]GC[ACT]^C
1644 GAGCCC
BsrDI 281 CATTGC GCAATG(2/0)
1206 CATTGC
BstYI 444 GGATCC [GA]^GATC[CT]
750 AGATCC
CelII 543 GCTCAGC GC^T[ACGT]AGC
1274 GCTCAGC
DraII 32 GGGACCC [GA]G^G[ACGT]CC[CT]
950 AGGCCCT
EcoO109I 32 GGGACCC [GA]G^G[ACGT]CC[CT]
950 AGGCCCT
EcoRI 187 GAATTC G^AATTC
301 GAATTC
HaeII 547 AGCGCC [GA]GCGC^[CT]
1535 AGCGCC
MaeI 235 CTAG C^TAG
1139 CTAG
MaeIII 1192 GTCAC ^GT[ACGT]AC
1201 GTCAC
MflI 444 GGATCC [GA]^GATC[CT]
750 AGATCC
NcoI 1253 CCATGG C^CATGG
1265 CCATGG
NdeI 403 CATATG CA^TATG
782 CATATG
PflMI 169 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
703 CCAGACTCTGG
SmlI 354 CTCAAG C^T[CT][GA]AG
1700 CTCAAG
Tsp45I 1192 GTCAC ^GT[GC]AC
1201 GTCAC
Van91I 169 CCAAGCCTTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
703 CCAGACTCTGG
XhoII 444 GGATCC [GA]^GATC[CT]
750 AGATCC
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AcsI AfaI AluI
AlwI AlwNI ApoI AvaII BbvI
BpmI BsaJI BsiYI BslI BsmAI
BsmFI BspMI BsrI BssKI BstAPI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DsaI EcoRII
EcoT14I Fnu4HI FokI HaeIII HapII
HhaI HinP1I HinfI HpaII HphI
ItaI MboI MboII MnlI MseI
MslI MspA1I MspI MvaI MvnI
MwoI NdeII NlaIII NlaIV NspI
PleI PstI PvuII RsaI Sau3AI
Sau96I ScrFI SfaNI SfcI StyI
TaqI Tru9I TseI Tsp509I TspRI
TthHB8I