(Circular) (Six-base) MAPSORT of: pFN21AB8732.seq Check: 8170 from: 1 to: 6433 pFN21AB8732 6433 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 3480 3480 Size: 6433 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4243 278 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3050 3776 1937 Size: 1113 726 4594 Fragments arranged by size: 4594 1113 726 Acc65I G'GTAC_C Cuts at: 3754 3754 Size: 6433 AclI AA'CG_TT Cuts at: 1563 4560 4933 1563 Size: 2997 373 3063 Fragments arranged by size: 3063 2997 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2972 4496 5880 6148 1632 Size: 1340 1524 1384 268 1917 Fragments arranged by size: 1917 1524 1384 1340 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6236 AflIII A'CryG_T Cuts at: 1203 5332 6309 1203 Size: 4129 977 1327 Fragments arranged by size: 4129 1327 977 AgeI A'CCGG_T Cuts at: 5264 5264 Size: 6433 AhdI GACnn_n'nnGTC Cuts at: 5162 5162 Size: 6433 AleI CACnn'nnGTG Cuts at: 1166 2429 3296 1166 Size: 1263 867 4303 Fragments arranged by size: 4303 1263 867 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 2420 1184 Size: 1236 5197 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2000 2032 2000 Size: 32 6401 AlwNI CAG_nnn'CTG Cuts at: 2068 2234 2714 2933 3043 3472 3520 3532 3559 Size: 166 480 219 110 429 48 12 27 Cuts at: 3559 4176 5748 2068 Size: 617 1572 2753 Fragments arranged by size: 2753 1572 617 480 429 219 166 110 48 27 12 ApaLI G'TGCA_C Cuts at: 4490 5646 4490 Size: 1156 5277 ApoI r'AATT_y Cuts at: 1493 2192 2199 2306 2569 3742 3968 4120 6229 Size: 699 7 107 263 1173 226 152 2109 Cuts at: 6229 1493 Size: 1697 Fragments arranged by size: 2109 1697 1173 699 263 226 152 107 7 AseI AT'TA_AT Cuts at: 160 1032 3507 4987 160 Size: 872 2475 1480 1606 Fragments arranged by size: 2475 1606 1480 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 AvaI C'yCGr_G Cuts at: 1870 1943 3758 1870 Size: 73 1815 4545 Fragments arranged by size: 4545 1815 73 AvrII C'CTAG_G Cuts at: 2239 3143 2239 Size: 904 5529 BamHI G'GATC_C Cuts at: 2449 3763 4130 2449 Size: 1314 367 4752 Fragments arranged by size: 4752 1314 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2579 3754 5209 618 Size: 325 217 627 792 1175 1455 1842 Fragments arranged by size: 1842 1455 1175 792 627 325 217 BanII G_rGCy'C Cuts at: 1391 3244 3653 3752 1391 Size: 1853 409 99 4072 Fragments arranged by size: 4072 1853 409 99 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5462 BbvCI CC'TCA_GC Cuts at: 2124 2276 2124 Size: 152 6281 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4624 4658 1903 Size: 34 2687 34 3678 Fragments arranged by size: 3678 2687 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4325 5535 4325 Size: 1210 5223 BclI T'GATC_A Cuts at: 1548 2460 1548 Size: 912 5521 BfrBI ATG'CAT Cuts at: 3412 3412 Size: 6433 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2963 5044 136 Size: 107 122 71 631 605 1291 2081 1525 Fragments arranged by size: 2081 1525 1291 631 605 122 107 71 BglII A'GATC_T Cuts at: 6428 6428 Size: 6433 BlpI GC'TnA_GC Cuts at: 2549 3280 3850 2549 Size: 731 570 5132 Fragments arranged by size: 5132 731 570 Bme1580I G_kGCm'C Cuts at: 1790 4494 5650 1790 Size: 2704 1156 2573 Fragments arranged by size: 2704 2573 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6433 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2311 2431 2551 Size: 416 53 83 399 24 894 120 120 Cuts at: 2551 4164 5122 442 Size: 1613 958 1753 Fragments arranged by size: 1753 1613 958 894 416 399 120 120 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6433 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2205 2595 2691 3113 3380 3479 3530 5093 Size: 966 390 96 422 267 99 51 1563 Cuts at: 5093 1239 Size: 2579 Fragments arranged by size: 2579 1563 966 422 390 267 99 96 51 Bpu10I CC'TnA_GC Cuts at: 2124 2276 2124 Size: 152 6281 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2344 3690 4558 5423 5721 2344 Size: 1346 868 865 298 3056 Fragments arranged by size: 3056 1346 868 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2863 5096 882 Size: 617 1364 2233 2219 Fragments arranged by size: 2233 2219 1364 617 BsaAI yAC'GTr Cuts at: 493 1204 4224 493 Size: 711 3020 2702 Fragments arranged by size: 3020 2702 711 BsaBI GATnn'nnATC Cuts at: 6427 6427 Size: 6433 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3047 4240 4622 5257 Size: 53 83 186 936 1514 1193 382 635 Cuts at: 5257 275 Size: 1451 Fragments arranged by size: 1514 1451 1193 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4866 5264 5538 5685 6096 4866 Size: 398 274 147 411 5203 Fragments arranged by size: 5203 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2203 2233 1178 Size: 30 165 30 800 30 5378 Fragments arranged by size: 5378 800 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2208 3582 1186 Size: 1022 1374 4037 Fragments arranged by size: 4037 1374 1022 BseYI C'CCAG_C Cuts at: 2520 3318 3654 5636 6132 2520 Size: 798 336 1982 496 2821 Fragments arranged by size: 2821 1982 798 496 336 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5860 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2034 3883 4644 5672 1540 Size: 275 186 33 1849 761 1028 2301 Fragments arranged by size: 2301 1849 1028 761 275 186 33 BsiHKAI G_wGCw'C Cuts at: 4494 4579 5650 4494 Size: 85 1071 5277 Fragments arranged by size: 5277 1071 85 BsmI GAATG_Cn' Cuts at: 3944 4037 3944 Size: 93 6340 Bsp1286I G_dGCh'C Cuts at: 1391 1790 3244 3653 3752 4494 4579 5650 1391 Size: 399 1454 409 99 742 85 1071 2174 Fragments arranged by size: 2174 1454 1071 742 409 399 99 85 BspEI T'CCGG_A Cuts at: 6096 6096 Size: 6433 BspHI T'CATG_A Cuts at: 4322 6052 4322 Size: 1730 4703 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2899 3480 3709 3788 844 Size: 2055 581 229 79 3489 Fragments arranged by size: 3489 2055 581 229 79 BsrBI CCG'CTC Cuts at: 1394 1823 4320 1394 Size: 429 2497 3507 Fragments arranged by size: 3507 2497 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2285 3210 4928 5102 65 Size: 1183 1037 925 1718 174 1396 Fragments arranged by size: 1718 1396 1183 1037 925 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2442 5077 5264 1700 Size: 110 632 2635 187 2869 Fragments arranged by size: 2869 2635 632 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6433 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6433 BssSI C'ACGA_G Cuts at: 2369 4493 5505 2369 Size: 2124 1012 3297 Fragments arranged by size: 3297 2124 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 3331 3379 3409 3433 3460 3472 3532 3559 Size: 2537 48 30 24 27 12 60 27 Cuts at: 3559 3568 794 Size: 9 3659 Fragments arranged by size: 3659 2537 60 48 30 27 27 24 12 9 BstBI TT'CG_AA Cuts at: 2196 5237 2196 Size: 3041 3392 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6433 BstYI r'GATC_y Cuts at: 1972 2449 2755 3763 4130 4515 4532 5984 6070 Size: 477 306 1008 367 385 17 1452 86 Cuts at: 6070 6082 6428 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 1008 477 385 367 346 306 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2025 3258 3270 513 Size: 552 939 21 1233 12 3676 Fragments arranged by size: 3676 1233 939 552 21 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5694 Fragments arranged by size: 5694 683 56 BtsI GCAGTG_nn' Cuts at: 2069 2404 3949 4743 4763 2069 Size: 335 1545 794 20 3739 Fragments arranged by size: 3739 1545 794 335 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6433 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6398 DraI TTT'AAA Cuts at: 3737 3737 Size: 6433 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 3046 5440 809 Size: 507 1730 2394 1802 Fragments arranged by size: 2394 1802 1730 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2031 3880 4769 Size: 54 1425 50 275 18 201 1849 889 Cuts at: 4769 6398 8 Size: 1629 43 Fragments arranged by size: 1849 1629 1425 889 275 201 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2031 3880 1537 Size: 275 219 1849 4090 Fragments arranged by size: 4090 1849 275 219 EarI CTCTTCn'nnn_ Cuts at: 1353 2652 4363 1353 Size: 1299 1711 3423 Fragments arranged by size: 3423 1711 1299 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 5020 5394 5540 387 Size: 1064 138 3431 374 146 1280 Fragments arranged by size: 3431 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2205 2595 2691 2972 3113 3380 3479 Size: 393 573 390 96 281 141 267 99 Cuts at: 3479 3530 4496 5093 5880 6148 1239 Size: 51 966 597 787 268 1524 Fragments arranged by size: 1524 966 787 597 573 393 390 281 268 267 141 99 96 51 EcoNI CCTnn'n_nnAGG Cuts at: 2238 2238 Size: 6433 EcoO109I rG'GnC_Cy Cuts at: 2038 2956 2038 Size: 918 5515 EcoRI G'AATT_C Cuts at: 2192 2306 3742 4120 2192 Size: 114 1436 378 4505 Fragments arranged by size: 4505 1436 378 114 FspI TGC'GCA Cuts at: 4939 4939 Size: 6433 HaeII r_GCGC'y Cuts at: 1678 2556 3544 5580 1678 Size: 878 988 2036 2531 Fragments arranged by size: 2531 2036 988 878 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3688 3720 5074 5106 5148 Size: 32 135 32 2116 32 1354 32 42 Cuts at: 5148 5180 1373 Size: 32 2626 Fragments arranged by size: 2626 2116 1354 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3051 3777 4023 669 Size: 988 281 1113 726 246 3079 Fragments arranged by size: 3079 1113 988 726 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6433 HpaI GTT'AAC Cuts at: 4023 4023 Size: 6433 KpnI G_GTAC'C Cuts at: 3758 3758 Size: 6433 MfeI C'AATT_G Cuts at: 3335 4032 3335 Size: 697 5736 MluI A'CGCG_T Cuts at: 6309 6309 Size: 6433 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5547 5731 1305 Size: 4242 184 2007 Fragments arranged by size: 4242 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4611 Fragments arranged by size: 4611 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2397 2429 2878 3296 4391 4750 Size: 648 843 388 32 449 418 1095 359 Cuts at: 4750 4909 518 Size: 159 2042 Fragments arranged by size: 2042 1095 843 648 449 418 388 359 159 32 MspA1I CmG'CkG Cuts at: 1606 2027 3520 3532 3550 3559 3849 4526 5674 Size: 421 1493 12 18 9 290 677 1148 Cuts at: 5674 5919 1606 Size: 245 2120 Fragments arranged by size: 2120 1493 1148 677 421 290 245 18 12 9 NcoI C'CATG_G Cuts at: 513 1065 2004 3258 3270 513 Size: 552 939 1254 12 3676 Fragments arranged by size: 3676 1254 939 552 12 NdeI CA'TA_TG Cuts at: 387 2409 2788 387 Size: 2022 379 4032 Fragments arranged by size: 4032 2022 379 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6433 NotI GC'GGCC_GC Cuts at: 3880 3880 Size: 6433 NsiI A_TGCA'T Cuts at: 3414 3414 Size: 6433 NspI r_CATG'y Cuts at: 1132 1229 2248 3226 3463 3571 3791 5336 1132 Size: 97 1019 978 237 108 220 1545 2229 Fragments arranged by size: 2229 1545 1019 978 237 220 108 97 PasI CC'CwG_GG Cuts at: 1348 2296 1348 Size: 948 5485 PciI A'CATG_T Cuts at: 5332 5332 Size: 6433 PflMI CCAn_nnn'nTGG Cuts at: 1108 2180 2714 1108 Size: 1072 534 4827 Fragments arranged by size: 4827 1072 534 PmeI GTTT'AAAC Cuts at: 3737 3737 Size: 6433 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4502 4534 6015 6047 4502 Size: 32 1481 32 4888 Fragments arranged by size: 4888 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2038 2038 Size: 6433 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6433 PsiI TTA'TAA Cuts at: 4003 4003 Size: 6433 PstI C_TGCA'G Cuts at: 830 3477 3525 3537 3555 3785 830 Size: 2647 48 12 18 230 3478 Fragments arranged by size: 3478 2647 230 48 18 12 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6433 PvuII CAG'CTG Cuts at: 3520 3532 3550 3559 3520 Size: 12 18 9 6394 Fragments arranged by size: 6394 18 12 9 SacII CC_GC'GG Cuts at: 2028 2028 Size: 6433 SalI G'TCGA_C Cuts at: 1936 3049 3775 1936 Size: 1113 726 4594 Fragments arranged by size: 4594 1113 726 SanDI GG'GwC_CC Cuts at: 2038 2038 Size: 6433 SbfI CC_TGCA'GG Cuts at: 3785 3785 Size: 6433 ScaI AGT'ACT Cuts at: 4681 6393 4681 Size: 1712 4721 SfcI C'TryA_G Cuts at: 826 1045 2083 3473 3521 3533 3551 3781 4916 Size: 219 1038 1390 48 12 18 230 1135 Cuts at: 4916 5597 5788 826 Size: 681 191 1471 Fragments arranged by size: 1471 1390 1135 1038 681 230 219 191 48 18 12 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6433 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6433 SmaI CCC'GGG Cuts at: 1872 3760 1872 Size: 1888 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2359 3705 4537 5438 5700 820 Size: 197 926 416 1346 832 901 262 1553 Fragments arranged by size: 1553 1346 926 901 832 416 262 197 SnaBI TAC'GTA Cuts at: 493 4224 493 Size: 3731 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6433 SphI G_CATG'C Cuts at: 1132 2248 3791 1132 Size: 1116 1543 3774 Fragments arranged by size: 3774 1543 1116 SspI AAT'ATT Cuts at: 5 52 4357 5 Size: 47 4305 2081 Fragments arranged by size: 4305 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6433 StyI C'CwwG_G Cuts at: 513 1065 2004 2179 2239 2890 3143 3258 3270 Size: 552 939 175 60 651 253 115 12 Cuts at: 3270 3872 513 Size: 602 3074 Fragments arranged by size: 3074 939 651 602 552 253 175 115 60 12 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2501 3308 4460 4477 4630 4815 5260 Size: 1255 921 807 1152 17 153 185 445 Cuts at: 5260 325 Size: 1498 Fragments arranged by size: 1498 1255 1152 921 807 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2254 4679 6391 Size: 274 80 33 51 1443 277 2425 1712 Cuts at: 6391 96 Size: 138 Fragments arranged by size: 2425 1712 1443 277 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2203 2235 6393 6425 2203 Size: 32 4158 32 2211 Fragments arranged by size: 4158 2211 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6433 XbaI T'CTAG_A Cuts at: 3769 3769 Size: 6433 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6433 XmaI C'CCGG_G Cuts at: 1870 3758 1870 Size: 1888 4545 XmnI GAAnn'nnTTC Cuts at: 4562 4562 Size: 6433 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4241 276 Size: 53 83 186 936 2707 2468 Fragments arranged by size: 2707 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI ApaI AscI BaeI BbeI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI PacI PfoI PmlI PspOMI PspXI PsrI RsrII SacI SapI SexAI SfiI SfoI SrfI SwaI XcmI