Restriction map for
pFN21AB8732
(Linear) (Six-base) MAPSORT of: KIBB8732.seq Check: 717 from: 1 to: 1728
KIBB8732.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 1475 1728
Size: 1475 253
AccI GT'mk_AC
Cuts at: 0 1045 1728
Size: 1045 683
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 967 1728
Size: 967 761
AleI CACnn'nnGTG
Cuts at: 0 424 1291 1728
Size: 424 867 437
Fragments arranged by size:
867 437 424
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 415 1728
Size: 415 1313
AloI GAACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 27 1728
Size: 27 1701
AlwNI CAG_nnn'CTG
Cuts at: 0 63 229 709 928 1038 1467 1515 1527
Size: 63 166 480 219 110 429 48 12
Cuts at: 1527 1554 1728
Size: 27 174
Fragments arranged by size:
480 429 219 174 166 110 63 48
27 12
ApoI r'AATT_y
Cuts at: 0 187 194 301 564 1728
Size: 187 7 107 263 1164
Fragments arranged by size:
1164 263 187 107 7
AseI AT'TA_AT
Cuts at: 0 1502 1728
Size: 1502 226
AvrII C'CTAG_G
Cuts at: 0 234 1138 1728
Size: 234 904 590
Fragments arranged by size:
904 590 234
BamHI G'GATC_C
Cuts at: 0 444 1728
Size: 444 1284
BanI G'GyrC_C
Cuts at: 0 574 1728
Size: 574 1154
BanII G_rGCy'C
Cuts at: 0 1239 1648 1728
Size: 1239 409 80
Fragments arranged by size:
1239 409 80
BbvCI CC'TCA_GC
Cuts at: 0 119 271 1728
Size: 119 152 1457
Fragments arranged by size:
1457 152 119
BclI T'GATC_A
Cuts at: 0 455 1728
Size: 455 1273
BfrBI ATG'CAT
Cuts at: 0 1407 1728
Size: 1407 321
BglI GCCn_nnn'nGGC
Cuts at: 0 958 1728
Size: 958 770
BlpI GC'TnA_GC
Cuts at: 0 544 1275 1728
Size: 544 731 453
Fragments arranged by size:
731 544 453
BmrI ACTGGGnnnn_n'
Cuts at: 0 306 426 546 1728
Size: 306 120 120 1182
Fragments arranged by size:
1182 306 120 120
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 590 686 1108 1375 1474 1525 1728
Size: 200 390 96 422 267 99 51 203
Fragments arranged by size:
422 390 267 203 200 99 96 51
Bpu10I CC'TnA_GC
Cuts at: 0 119 271 1728
Size: 119 152 1457
Fragments arranged by size:
1457 152 119
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 339 1685 1728
Size: 339 1346 43
Fragments arranged by size:
1346 339 43
BsaI GGTCTCn'nnnn_
Cuts at: 0 858 1728
Size: 858 870
BsaHI Gr'CG_yC
Cuts at: 0 1042 1728
Size: 1042 686
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 198 228 1728
Size: 198 30 1500
Fragments arranged by size:
1500 198 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 203 1577 1728
Size: 203 1374 151
Fragments arranged by size:
1374 203 151
BseYI C'CCAG_C
Cuts at: 0 515 1313 1649 1728
Size: 515 798 336 79
Fragments arranged by size:
798 515 336 79
BsiEI CG_ry'CG
Cuts at: 0 29 1728
Size: 29 1699
Bsp1286I G_dGCh'C
Cuts at: 0 1239 1648 1728
Size: 1239 409 80
Fragments arranged by size:
1239 409 80
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 894 1475 1704 1728
Size: 894 581 229 24
Fragments arranged by size:
894 581 229 24
BsrDI GCAATG_nn'
Cuts at: 0 280 1205 1728
Size: 280 925 523
Fragments arranged by size:
925 523 280
BsrFI r'CCGG_y
Cuts at: 0 437 1728
Size: 437 1291
BssSI C'ACGA_G
Cuts at: 0 364 1728
Size: 364 1364
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1326 1374 1404 1428 1455 1467 1527 1554
Size: 1326 48 30 24 27 12 60 27
Cuts at: 1554 1563 1728
Size: 9 165
Fragments arranged by size:
1326 165 60 48 30 27 27 24
12 9
BstBI TT'CG_AA
Cuts at: 0 191 1728
Size: 191 1537
BstYI r'GATC_y
Cuts at: 0 444 750 1728
Size: 444 306 978
Fragments arranged by size:
978 444 306
BtgI C'CryG_G
Cuts at: 0 20 1253 1265 1728
Size: 20 1233 12 463
Fragments arranged by size:
1233 463 20 12
BtsI GCAGTG_nn'
Cuts at: 0 64 399 1728
Size: 64 335 1329
Fragments arranged by size:
1329 335 64
DrdI GACnn_nn'nnGTC
Cuts at: 0 1041 1728
Size: 1041 687
EaeI y'GGCC_r
Cuts at: 0 26 1728
Size: 26 1702
EagI C'GGCC_G
Cuts at: 0 26 1728
Size: 26 1702
EarI CTCTTCn'nnn_
Cuts at: 0 647 1728
Size: 647 1081
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 200 590 686 967 1108 1375 1474 1525
Size: 200 390 96 281 141 267 99 51
Cuts at: 1525 1728
Size: 203
Fragments arranged by size:
390 281 267 203 200 141 99 96
51
EcoNI CCTnn'n_nnAGG
Cuts at: 0 233 1728
Size: 233 1495
EcoO109I rG'GnC_Cy
Cuts at: 0 33 951 1728
Size: 33 918 777
Fragments arranged by size:
918 777 33
EcoRI G'AATT_C
Cuts at: 0 187 301 1728
Size: 187 114 1427
Fragments arranged by size:
1427 187 114
HaeII r_GCGC'y
Cuts at: 0 551 1539 1728
Size: 551 988 189
Fragments arranged by size:
988 551 189
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 1683 1715 1728
Size: 1683 32 13
Fragments arranged by size:
1683 32 13
HincII GTy'rAC
Cuts at: 0 1046 1728
Size: 1046 682
MfeI C'AATT_G
Cuts at: 0 1330 1728
Size: 1330 398
MslI CAynn'nnrTG
Cuts at: 0 392 424 873 1291 1728
Size: 392 32 449 418 437
Fragments arranged by size:
449 437 418 392 32
MspA1I CmG'CkG
Cuts at: 0 22 1515 1527 1545 1554 1728
Size: 22 1493 12 18 9 174
Fragments arranged by size:
1493 174 22 18 12 9
NcoI C'CATG_G
Cuts at: 0 1253 1265 1728
Size: 1253 12 463
Fragments arranged by size:
1253 463 12
NdeI CA'TA_TG
Cuts at: 0 404 783 1728
Size: 404 379 945
Fragments arranged by size:
945 404 379
NsiI A_TGCA'T
Cuts at: 0 1409 1728
Size: 1409 319
NspI r_CATG'y
Cuts at: 0 243 1221 1458 1566 1728
Size: 243 978 237 108 162
Fragments arranged by size:
978 243 237 162 108
PasI CC'CwG_GG
Cuts at: 0 291 1728
Size: 291 1437
PflMI CCAn_nnn'nTGG
Cuts at: 0 175 709 1728
Size: 175 534 1019
Fragments arranged by size:
1019 534 175
PpuMI rG'GwC_Cy
Cuts at: 0 33 1728
Size: 33 1695
PstI C_TGCA'G
Cuts at: 0 1472 1520 1532 1550 1728
Size: 1472 48 12 18 178
Fragments arranged by size:
1472 178 48 18 12
PvuII CAG'CTG
Cuts at: 0 1515 1527 1545 1554 1728
Size: 1515 12 18 9 174
Fragments arranged by size:
1515 174 18 12 9
SacII CC_GC'GG
Cuts at: 0 23 1728
Size: 23 1705
SalI G'TCGA_C
Cuts at: 0 1044 1728
Size: 1044 684
SanDI GG'GwC_CC
Cuts at: 0 33 1728
Size: 33 1695
SfcI C'TryA_G
Cuts at: 0 78 1468 1516 1528 1546 1728
Size: 78 1390 48 12 18 182
Fragments arranged by size:
1390 182 78 48 18 12
SmlI C'TyrA_G
Cuts at: 0 354 1700 1728
Size: 354 1346 28
Fragments arranged by size:
1346 354 28
SphI G_CATG'C
Cuts at: 0 243 1728
Size: 243 1485
StyI C'CwwG_G
Cuts at: 0 174 234 885 1138 1253 1265 1728
Size: 174 60 651 253 115 12 463
Fragments arranged by size:
651 463 253 174 115 60 12
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 496 1303 1728
Size: 496 807 425
Fragments arranged by size:
807 496 425
TatI w'GTAC_w
Cuts at: 0 249 1728
Size: 249 1479
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 198 230 1728
Size: 198 32 1498
Fragments arranged by size:
1498 198 32
Enzymes that do cut:
AarI AccI AcuI AleI AlfI AloI AlwNI ApoI
AseI AvrII BamHI BanI BanII BbvCI BclI BfrBI
BglI BlpI BmrI BpmI Bpu10I BpuEI BsaI BsaHI
BsaXI BseRI BseYI BsiEI Bsp1286I BspMI BsrDI BsrFI
BssSI BstAPI BstBI BstYI BtgI BtsI DrdI EaeI
EagI EarI Eco57MI EcoNI EcoO109I EcoRI HaeII Hin4I
HincII MfeI MslI MspA1I NcoI NdeI NsiI NspI
PasI PflMI PpuMI PstI PvuII SacII SalI SanDI
SfcI SmlI SphI StyI TaqII TatI TstI
Enzymes that do not cut:
AatII Acc65I AclI AfeI AflII AflIII AgeI AhdI
ApaI ApaLI AscI AsiSI AvaI BaeI BbeI BbsI
BcgI BciVI BglII Bme1580I BmgBI BmtI BplI BsaAI
BsaBI BsaWI BsgI BsiHKAI BsiWI BsmI BsmBI BspEI
BspHI BsrBI BsrGI BssHII BstEII BstXI BstZ17I Bsu36I
BtgZI ClaI CspCI DraI DraIII EciI EcoICRI EcoRV
FalI FseI FspI FspAI HindIII HpaI KasI KpnI
MluI MmeI MscI NaeI NarI NgoMIV NheI NotI
NruI PacI PciI PfoI PmeI PmlI PpiI PshAI
PsiI PspOMI PspXI PsrI PvuI RsrII SacI SapI
SbfI ScaI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI Tth111I XbaI
XcmI XhoI XmaI XmnI ZraI