Restriction map for
pFN21AB8731
(Circular) (Six-base) MAPSORT of: pFN21AB8731.seq Check: 7162 from: 1 to: 6763
pFN21AB8731 6763 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 4573 278
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 4106 1937
Size: 2169 4594
Acc65I G'GTAC_C
Cuts at: 4084 4084
Size: 6763
AclI AA'CG_TT
Cuts at: 1563 4890 5263 1563
Size: 3327 373 3063
Fragments arranged by size:
3327 3063 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2349 2656 2824 3244 3496 4826 6210 6478
Size: 717 307 168 420 252 1330 1384 268
Cuts at: 6478 1632
Size: 1917
Fragments arranged by size:
1917 1384 1330 717 420 307 268 252
168
AflII C'TTAA_G
Cuts at: 820 1017 820
Size: 197 6566
AflIII A'CryG_T
Cuts at: 1203 3118 5662 6639 1203
Size: 1915 2544 977 1327
Fragments arranged by size:
2544 1915 1327 977
AgeI A'CCGG_T
Cuts at: 5594 5594
Size: 6763
AhdI GACnn_n'nnGTC
Cuts at: 5492 5492
Size: 6763
AleI CACnn'nnGTG
Cuts at: 1166 3219 3723 1166
Size: 2053 504 4206
Fragments arranged by size:
4206 2053 504
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6763
AlwNI CAG_nnn'CTG
Cuts at: 4506 6078 4506
Size: 1572 5191
ApaLI G'TGCA_C
Cuts at: 3788 4820 5976 3788
Size: 1032 1156 4575
Fragments arranged by size:
4575 1156 1032
ApoI r'AATT_y
Cuts at: 1493 2309 2357 2622 2706 2790 2874 3126 3294
Size: 816 48 265 84 84 84 252 168
Cuts at: 3294 3378 3462 4050 4072 4298 4450 6559 1493
Size: 84 84 588 22 226 152 2109 1697
Fragments arranged by size:
2109 1697 816 588 265 252 226 168
152 84 84 84 84 84 48 22
AseI AT'TA_AT
Cuts at: 160 1032 5317 160
Size: 872 4285 1606
Fragments arranged by size:
4285 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
AvaI C'yCGr_G
Cuts at: 1870 1943 4088 1870
Size: 73 2145 4545
Fragments arranged by size:
4545 2145 73
BamHI G'GATC_C
Cuts at: 2080 4093 4460 2080
Size: 2013 367 4383
Fragments arranged by size:
4383 2013 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 4084 5539 618
Size: 325 217 627 2297 1455 1842
Fragments arranged by size:
2297 1842 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 4082 1391
Size: 2691 4072
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5792
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 4954 4988 1903
Size: 34 3017 34 3678
Fragments arranged by size:
3678 3017 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 4655 5865 4655
Size: 1210 5553
BclI T'GATC_A
Cuts at: 1548 2863 1548
Size: 1315 5448
BfrBI ATG'CAT
Cuts at: 2816 2984 2816
Size: 168 6595
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 5374 136
Size: 107 122 71 631 605 3702 1525
Fragments arranged by size:
3702 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 6758 6758
Size: 6763
BlpI GC'TnA_GC
Cuts at: 3187 4180 3187
Size: 993 5770
Bme1580I G_kGCm'C
Cuts at: 1790 3792 4824 5980 1790
Size: 2002 1032 1156 2573
Fragments arranged by size:
2573 2002 1156 1032
BmgBI CAC'GTC
Cuts at: 1318 1318
Size: 6763
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 2976 3396 4494
Size: 416 53 83 399 24 1559 420 1098
Cuts at: 4494 5452 442
Size: 958 1753
Fragments arranged by size:
1753 1559 1098 958 420 416 399 83
53 24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6763
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2652 2820 2904 3492 3576 5423 1239
Size: 1413 168 84 588 84 1847 2579
Fragments arranged by size:
2579 1847 1413 588 168 84 84
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 4888 5753 6051 4888
Size: 865 298 5600
Fragments arranged by size:
5600 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 5426 882
Size: 617 3927 2219
Fragments arranged by size:
3927 2219 617
BsaAI yAC'GTr
Cuts at: 493 1204 4554 493
Size: 711 3350 2702
Fragments arranged by size:
3350 2702 711
BsaBI GATnn'nnATC
Cuts at: 2265 6757 2265
Size: 4492 2271
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 4570 4952 5587 275
Size: 53 83 186 936 3037 382 635 1451
Fragments arranged by size:
3037 1451 936 635 382 186 83 53
BsaWI w'CCGG_w
Cuts at: 2103 5196 5594 5868 6015 6426 2103
Size: 3093 398 274 147 411 2440
Fragments arranged by size:
3093 2440 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 6538
Fragments arranged by size:
6538 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2078 1186
Size: 892 5871
BseYI C'CCAG_C
Cuts at: 5966 6462 5966
Size: 496 6267
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 1163
Size: 573 6190
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 4213 4974 6002 1540
Size: 275 186 2212 761 1028 2301
Fragments arranged by size:
2301 2212 1028 761 275 186
BsiHKAI G_wGCw'C
Cuts at: 3692 3792 4824 4909 5980 3692
Size: 100 1032 85 1071 4475
Fragments arranged by size:
4475 1071 1032 100 85
BsmI GAATG_Cn'
Cuts at: 2921 3251 3494 4274 4367 2921
Size: 330 243 780 93 5317
Fragments arranged by size:
5317 780 330 243 93
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 3692 3792 4082 4824 4909 5980 1391
Size: 399 1902 100 290 742 85 1071 2174
Fragments arranged by size:
2174 1902 1071 742 399 290 100 85
BspEI T'CCGG_A
Cuts at: 6426 6426
Size: 6763
BspHI T'CATG_A
Cuts at: 2866 3454 4652 6382 2866
Size: 588 1198 1730 3247
Fragments arranged by size:
3247 1730 1198 588
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 4118 844
Size: 3274 3489
BsrBI CCG'CTC
Cuts at: 1394 1823 4650 1394
Size: 429 2827 3507
Fragments arranged by size:
3507 2827 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 2347 2943 3447 3499 5258 5432 65
Size: 1183 1099 596 504 52 1759 174 1396
Fragments arranged by size:
1759 1396 1183 1099 596 504 174 52
BsrFI r'CCGG_y
Cuts at: 1700 1810 5407 5594 1700
Size: 110 3597 187 2869
Fragments arranged by size:
3597 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 3199 96
Size: 3103 3660
BssHII G'CGCG_C
Cuts at: 1925 1925
Size: 6763
BssSI C'ACGA_G
Cuts at: 4823 5835 4823
Size: 1012 5751
BstAPI GCAn_nnn'nTGC
Cuts at: 794 3026 794
Size: 2232 4531
BstBI TT'CG_AA
Cuts at: 5567 5567
Size: 6763
BstEII G'GTnAC_C
Cuts at: 1184 1184
Size: 6763
BstYI r'GATC_y
Cuts at: 1972 2080 4093 4460 4845 4862 6314 6400 6412
Size: 108 2013 367 385 17 1452 86 12
Cuts at: 6412 6758 1972
Size: 346 1977
Fragments arranged by size:
2013 1977 1452 385 367 346 108 86
17 12
BtgI C'CryG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 5075
Fragments arranged by size:
5075 1136 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 6024
Fragments arranged by size:
6024 683 56
BtsI GCAGTG_nn'
Cuts at: 3692 4279 5073 5093 3692
Size: 587 794 20 5362
Fragments arranged by size:
5362 794 587 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6763
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 3481 3516 529
Size: 35 2917 35 3776
Fragments arranged by size:
3776 2917 35 35
DraI TTT'AAA
Cuts at: 4067 4067
Size: 6763
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5770 809
Size: 507 4454 1802
Fragments arranged by size:
4454 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 4210 5099 6728
Size: 54 1425 50 275 18 2380 889 1629
Cuts at: 6728 8
Size: 43
Fragments arranged by size:
2380 1629 1425 889 275 54 50 43
18
EagI C'GGCC_G
Cuts at: 1537 1812 4210 1537
Size: 275 2398 4090
Fragments arranged by size:
4090 2398 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2066 3036 4693 1353
Size: 713 970 1657 3423
Fragments arranged by size:
3423 1657 970 713
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 5350 5724 5870 387
Size: 1064 138 3761 374 146 1280
Fragments arranged by size:
3761 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2349 2652 2656 2820 2824 2904 3244
Size: 393 717 303 4 164 4 80 340
Cuts at: 3244 3492 3496 3576 4826 5423 6210 6478 1239
Size: 248 4 80 1250 597 787 268 1524
Fragments arranged by size:
1524 1250 787 717 597 393 340 303
268 248 164 80 80 4 4 4
EcoRI G'AATT_C
Cuts at: 2622 2790 3126 3378 4050 4072 4450 2622
Size: 168 336 252 672 22 378 4935
Fragments arranged by size:
4935 672 378 336 252 168 22
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 3292 3544 3292
Size: 252 6511
FspI TGC'GCA
Cuts at: 5269 5269
Size: 6763
HaeII r_GCGC'y
Cuts at: 1678 5910 1678
Size: 4232 2531
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2065 2097 2394 2426 3343
Size: 32 135 32 493 32 297 32 917
Cuts at: 3343 3375 5404 5436 5478 5510 1373
Size: 32 2029 32 42 32 2626
Fragments arranged by size:
2626 2029 917 493 297 135 42 32
32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 4107 4353 669
Size: 988 281 2169 246 3079
Fragments arranged by size:
3079 2169 988 281 246
HindIII A'AGCT_T
Cuts at: 748 2751 3339 748
Size: 2003 588 4172
Fragments arranged by size:
4172 2003 588
HpaI GTT'AAC
Cuts at: 4353 4353
Size: 6763
KpnI G_GTAC'C
Cuts at: 4088 4088
Size: 6763
MfeI C'AATT_G
Cuts at: 4362 4362
Size: 6763
MluI A'CGCG_T
Cuts at: 6639 6639
Size: 6763
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 5877 6061 1305
Size: 4572 184 2007
Fragments arranged by size:
4572 2007 184
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4941
Fragments arranged by size:
4941 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2466 2762 3135 3219 3723 3807 3891
Size: 648 1300 296 373 84 504 84 84
Cuts at: 3891 3975 4059 4721 5080 5239 518
Size: 84 84 662 359 159 2042
Fragments arranged by size:
2042 1300 662 648 504 373 359 296
159 84 84 84 84 84
MspA1I CmG'CkG
Cuts at: 1606 4179 4856 6004 6249 1606
Size: 2573 677 1148 245 2120
Fragments arranged by size:
2573 2120 1148 677 245
NcoI C'CATG_G
Cuts at: 513 1065 2201 513
Size: 552 1136 5075
Fragments arranged by size:
5075 1136 552
NdeI CA'TA_TG
Cuts at: 387 2524 3036 3204 3708 3960 387
Size: 2137 512 168 504 252 3190
Fragments arranged by size:
3190 2137 512 504 252 168
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6763
NotI GC'GGCC_GC
Cuts at: 4210 4210
Size: 6763
NsiI A_TGCA'T
Cuts at: 2818 2986 2818
Size: 168 6595
NspI r_CATG'y
Cuts at: 1132 1229 2820 2988 3122 4121 5666 1132
Size: 97 1591 168 134 999 1545 2229
Fragments arranged by size:
2229 1591 1545 999 168 134 97
PasI CC'CwG_GG
Cuts at: 1348 2017 1348
Size: 669 6094
PciI A'CATG_T
Cuts at: 3118 5662 3118
Size: 2544 4219
PflMI CCAn_nnn'nTGG
Cuts at: 1108 1108
Size: 6763
PmeI GTTT'AAAC
Cuts at: 4067 4067
Size: 6763
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4832 4864 6345 6377 4832
Size: 32 1481 32 5218
Fragments arranged by size:
5218 1481 32 32
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6763
PsiI TTA'TAA
Cuts at: 2703 3066 4333 2703
Size: 363 1267 5133
Fragments arranged by size:
5133 1267 363
PstI C_TGCA'G
Cuts at: 830 4115 830
Size: 3285 3478
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6763
SalI G'TCGA_C
Cuts at: 1936 4105 1936
Size: 2169 4594
SapI GCTCTTCn'nnn_
Cuts at: 3036 3036
Size: 6763
SbfI CC_TGCA'GG
Cuts at: 4115 4115
Size: 6763
ScaI AGT'ACT
Cuts at: 5011 6723 5011
Size: 1712 5051
SfcI C'TryA_G
Cuts at: 826 1045 2659 3910 4111 5246 5927 6118 826
Size: 219 1614 1251 201 1135 681 191 1471
Fragments arranged by size:
1614 1471 1251 1135 681 219 201 191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6763
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6763
SmaI CCC'GGG
Cuts at: 1872 4090 1872
Size: 2218 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 4867 5768 6030 820
Size: 197 926 2924 901 262 1553
Fragments arranged by size:
2924 1553 926 901 262 197
SnaBI TAC'GTA
Cuts at: 493 4554 493
Size: 4061 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6763
SphI G_CATG'C
Cuts at: 1132 4121 1132
Size: 2989 3774
SspI AAT'ATT
Cuts at: 5 52 4687 5
Size: 47 4635 2081
Fragments arranged by size:
4635 2081 47
StuI AGG'CCT
Cuts at: 1517 1517
Size: 6763
StyI C'CwwG_G
Cuts at: 513 1065 2201 4202 513
Size: 552 1136 2001 3074
Fragments arranged by size:
3074 2001 1136 552
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2194 4790 4807 4960 5145 5590 325
Size: 1255 614 2596 17 153 185 445 1498
Fragments arranged by size:
2596 1498 1255 614 445 185 153 17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 3199 5009 6721
Size: 274 80 33 51 1443 1222 1810 1712
Cuts at: 6721 96
Size: 138
Fragments arranged by size:
1810 1712 1443 1222 274 138 80 51
33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6723 6755 6723
Size: 32 6731
Tth111I GACn'n_nGTC
Cuts at: 1316 2011 1316
Size: 695 6068
XbaI T'CTAG_A
Cuts at: 4099 4099
Size: 6763
XhoI C'TCGA_G
Cuts at: 1943 1943
Size: 6763
XmaI C'CCGG_G
Cuts at: 1870 4088 1870
Size: 2218 4545
XmnI GAAnn'nnTTC
Cuts at: 2837 2874 3275 3462 3761 3902 4892 2837
Size: 37 401 187 299 141 990 4708
Fragments arranged by size:
4708 990 401 299 187 141 37
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 4571 276
Size: 53 83 186 936 3037 2468
Fragments arranged by size:
3037 2468 936 186 83 53
Enzymes that do cut:
AatII AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI
AvaI BamHI BanI BanII BbsI BcgI BciVI BclI
BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI
BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI
BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI
BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI
BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI
CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI
EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PmeI PpiI PshAI PsiI PstI PvuI SalI SapI
SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI
SpeI SphI SspI StuI StyI TaqII TatI TstI
Tth111I XbaI XhoI XmaI XmnI ZraI
Enzymes that do not cut:
AarI AfeI AloI ApaI AscI AvrII BaeI BbeI
BbvCI BplI Bpu10I BsiWI BsmBI BstXI BstZ17I Bsu36I
DraIII EcoICRI EcoNI EcoO109I EcoRV FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PfoI PmlI PpuMI
PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI
SexAI SfiI SfoI SrfI SwaI XcmI