Restriction map for
pFN21AB8731
(Linear) (Six-base) MAPSORT of: KIBB8731.seq Check: 3745 from: 1 to: 2058
KIBB8731.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 651 819 1239 1491 2058
Size: 344 307 168 420 252 567
Fragments arranged by size:
567 420 344 307 252 168
AflIII A'CryG_T
Cuts at: 0 1113 2058
Size: 1113 945
AleI CACnn'nnGTG
Cuts at: 0 1214 1718 2058
Size: 1214 504 340
Fragments arranged by size:
1214 504 340
ApaLI G'TGCA_C
Cuts at: 0 1783 2058
Size: 1783 275
ApoI r'AATT_y
Cuts at: 0 304 352 617 701 785 869 1121 1289
Size: 304 48 265 84 84 84 252 168
Cuts at: 1289 1373 1457 2045 2058
Size: 84 84 588 13
Fragments arranged by size:
588 304 265 252 168 84 84 84
84 84 48 13
BamHI G'GATC_C
Cuts at: 0 75 2058
Size: 75 1983
BclI T'GATC_A
Cuts at: 0 858 2058
Size: 858 1200
BfrBI ATG'CAT
Cuts at: 0 811 979 2058
Size: 811 168 1079
Fragments arranged by size:
1079 811 168
BlpI GC'TnA_GC
Cuts at: 0 1182 2058
Size: 1182 876
Bme1580I G_kGCm'C
Cuts at: 0 1787 2058
Size: 1787 271
BmrI ACTGGGnnnn_n'
Cuts at: 0 971 1391 2058
Size: 971 420 667
Fragments arranged by size:
971 667 420
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 647 815 899 1487 1571 2058
Size: 647 168 84 588 84 487
Fragments arranged by size:
647 588 487 168 84 84
BsaBI GATnn'nnATC
Cuts at: 0 260 2058
Size: 260 1798
BsaWI w'CCGG_w
Cuts at: 0 98 2058
Size: 98 1960
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 73 2058
Size: 73 1985
BsiHKAI G_wGCw'C
Cuts at: 0 1687 1787 2058
Size: 1687 100 271
Fragments arranged by size:
1687 271 100
BsmI GAATG_Cn'
Cuts at: 0 916 1246 1489 2058
Size: 916 330 243 569
Fragments arranged by size:
916 569 330 243
Bsp1286I G_dGCh'C
Cuts at: 0 1687 1787 2058
Size: 1687 100 271
Fragments arranged by size:
1687 271 100
BspHI T'CATG_A
Cuts at: 0 861 1449 2058
Size: 861 588 609
Fragments arranged by size:
861 609 588
BsrDI GCAATG_nn'
Cuts at: 0 342 938 1442 1494 2058
Size: 342 596 504 52 564
Fragments arranged by size:
596 564 504 342 52
BsrGI T'GTAC_A
Cuts at: 0 1194 2058
Size: 1194 864
BstAPI GCAn_nnn'nTGC
Cuts at: 0 1021 2058
Size: 1021 1037
BstYI r'GATC_y
Cuts at: 0 75 2058
Size: 75 1983
BtgI C'CryG_G
Cuts at: 0 196 2058
Size: 196 1862
BtsI GCAGTG_nn'
Cuts at: 0 1687 2058
Size: 1687 371
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 0 1476 1511 2058
Size: 1476 35 547
Fragments arranged by size:
1476 547 35
EarI CTCTTCn'nnn_
Cuts at: 0 61 1031 2058
Size: 61 970 1027
Fragments arranged by size:
1027 970 61
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 344 647 651 815 819 899 1239 1487
Size: 344 303 4 164 4 80 340 248
Cuts at: 1487 1491 1571 2058
Size: 4 80 487
Fragments arranged by size:
487 344 340 303 248 164 80 80
4 4 4
EcoRI G'AATT_C
Cuts at: 0 617 785 1121 1373 2045 2058
Size: 617 168 336 252 672 13
Fragments arranged by size:
672 617 336 252 168 13
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 1287 1539 2058
Size: 1287 252 519
Fragments arranged by size:
1287 519 252
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 60 92 389 421 1338 1370 2058
Size: 60 32 297 32 917 32 688
Fragments arranged by size:
917 688 297 60 32 32 32
HindIII A'AGCT_T
Cuts at: 0 746 1334 2058
Size: 746 588 724
Fragments arranged by size:
746 724 588
MslI CAynn'nnrTG
Cuts at: 0 461 757 1130 1214 1718 1802 1886 1970
Size: 461 296 373 84 504 84 84 84
Cuts at: 1970 2058
Size: 88
Fragments arranged by size:
504 461 373 296 88 84 84 84
84
NcoI C'CATG_G
Cuts at: 0 196 2058
Size: 196 1862
NdeI CA'TA_TG
Cuts at: 0 519 1031 1199 1703 1955 2058
Size: 519 512 168 504 252 103
Fragments arranged by size:
519 512 504 252 168 103
NsiI A_TGCA'T
Cuts at: 0 813 981 2058
Size: 813 168 1077
Fragments arranged by size:
1077 813 168
NspI r_CATG'y
Cuts at: 0 815 983 1117 2058
Size: 815 168 134 941
Fragments arranged by size:
941 815 168 134
PasI CC'CwG_GG
Cuts at: 0 12 2058
Size: 12 2046
PciI A'CATG_T
Cuts at: 0 1113 2058
Size: 1113 945
PsiI TTA'TAA
Cuts at: 0 698 1061 2058
Size: 698 363 997
Fragments arranged by size:
997 698 363
SapI GCTCTTCn'nnn_
Cuts at: 0 1031 2058
Size: 1031 1027
SfcI C'TryA_G
Cuts at: 0 654 1905 2058
Size: 654 1251 153
Fragments arranged by size:
1251 654 153
StyI C'CwwG_G
Cuts at: 0 196 2058
Size: 196 1862
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 189 2058
Size: 189 1869
TatI w'GTAC_w
Cuts at: 0 1194 2058
Size: 1194 864
Tth111I GACn'n_nGTC
Cuts at: 0 6 2058
Size: 6 2052
XmnI GAAnn'nnTTC
Cuts at: 0 832 869 1270 1457 1756 1897 2058
Size: 832 37 401 187 299 141 161
Fragments arranged by size:
832 401 299 187 161 141 37
Enzymes that do cut:
AcuI AflIII AleI ApaLI ApoI BamHI BclI BfrBI
BlpI Bme1580I BmrI BpmI BsaBI BsaWI BseRI BsiHKAI
BsmI Bsp1286I BspHI BsrDI BsrGI BstAPI BstYI BtgI
BtsI CspCI EarI Eco57MI EcoRI FalI Hin4I HindIII
MslI NcoI NdeI NsiI NspI PasI PciI PsiI
SapI SfcI StyI TaqII TatI Tth111I XmnI
Enzymes that do not cut:
AarI AatII AccI Acc65I AclI AfeI AflII AgeI
AhdI AlfI AloI AlwNI ApaI AscI AseI AsiSI
AvaI AvrII BaeI BanI BanII BbeI BbsI BbvCI
BcgI BciVI BglI BglII BmgBI BmtI BplI Bpu10I
BpuEI BsaI BsaAI BsaHI BsaXI BseYI BsgI BsiEI
BsiWI BsmBI BspEI BspMI BsrBI BsrFI BssHII BssSI
BstBI BstEII BstXI BstZ17I Bsu36I BtgZI ClaI DraI
DraIII DrdI EaeI EagI EciI EcoICRI EcoNI EcoO109I
EcoRV FseI FspI FspAI HaeII HincII HpaI KasI
KpnI MfeI MluI MmeI MscI MspA1I NaeI NarI
NgoMIV NheI NotI NruI PacI PflMI PfoI PmeI
PmlI PpiI PpuMI PshAI PspOMI PspXI PsrI PstI
PvuI PvuII RsrII SacI SacII SalI SanDI SbfI
ScaI SexAI SfiI SfoI SgfI SgrAI SmaI SmlI
SnaBI SpeI SphI SrfI SspI StuI SwaI TstI
XbaI XcmI XhoI XmaI ZraI