Restriction map for
pFN21AB8729
(Linear) (Six-base) MAPSORT of: KIBB8729.seq Check: 6417 from: 1 to: 2523
KIBB8729.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 16 262 582 867 1427 2351 2523
Size: 16 246 320 285 560 924 172
Fragments arranged by size:
924 560 320 285 246 172 16
AatII G_ACGT'C
Cuts at: 0 604 1318 1769 2523
Size: 604 714 451 754
Fragments arranged by size:
754 714 604 451
AccI GT'mk_AC
Cuts at: 0 1769 2026 2523
Size: 1769 257 497
Fragments arranged by size:
1769 497 257
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 598 1699 2023 2523
Size: 598 1101 324 500
Fragments arranged by size:
1101 598 500 324
AfeI AGC'GCT
Cuts at: 0 2464 2523
Size: 2464 59
AflIII A'CryG_T
Cuts at: 0 866 2523
Size: 866 1657
AhdI GACnn_n'nnGTC
Cuts at: 0 1050 2055 2523
Size: 1050 1005 468
Fragments arranged by size:
1050 1005 468
AleI CACnn'nnGTG
Cuts at: 0 416 2443 2523
Size: 416 2027 80
Fragments arranged by size:
2027 416 80
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2077 2523
Size: 2077 446
AlwNI CAG_nnn'CTG
Cuts at: 0 171 597 1506 1839 2523
Size: 171 426 909 333 684
Fragments arranged by size:
909 684 426 333 171
ApaI G_GGCC'C
Cuts at: 0 1279 2113 2319 2523
Size: 1279 834 206 204
Fragments arranged by size:
1279 834 206 204
ApaLI G'TGCA_C
Cuts at: 0 1180 2523
Size: 1180 1343
ApoI r'AATT_y
Cuts at: 0 1807 2523
Size: 1807 716
AvaI C'yCGr_G
Cuts at: 0 479 860 1138 1230 2523
Size: 479 381 278 92 1293
Fragments arranged by size:
1293 479 381 278 92
BamHI G'GATC_C
Cuts at: 0 1019 1574 2523
Size: 1019 555 949
Fragments arranged by size:
1019 949 555
BanI G'GyrC_C
Cuts at: 0 96 451 1415 1945 2262 2505 2523
Size: 96 355 964 530 317 243 18
Fragments arranged by size:
964 530 355 317 243 96 18
BanII G_rGCy'C
Cuts at: 0 361 1279 1726 2113 2319 2495 2523
Size: 361 918 447 387 206 176 28
Fragments arranged by size:
918 447 387 361 206 176 28
BbeI G_GCGC'C
Cuts at: 0 455 2266 2523
Size: 455 1811 257
Fragments arranged by size:
1811 455 257
BbsI GAAGACnn'nnnn_
Cuts at: 0 1203 2523
Size: 1203 1320
BbvCI CC'TCA_GC
Cuts at: 0 1915 2512 2523
Size: 1915 597 11
Fragments arranged by size:
1915 597 11
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 0 2224 2258 2523
Size: 2224 34 265
Fragments arranged by size:
2224 265 34
BciVI GTATCCnnnnn_n'
Cuts at: 0 2034 2523
Size: 2034 489
BclI T'GATC_A
Cuts at: 0 1787 2523
Size: 1787 736
BglI GCCn_nnn'nGGC
Cuts at: 0 588 1588 2523
Size: 588 1000 935
Fragments arranged by size:
1000 935 588
BglII A'GATC_T
Cuts at: 0 971 1745 2523
Size: 971 774 778
Fragments arranged by size:
971 778 774
BlpI GC'TnA_GC
Cuts at: 0 1354 2523
Size: 1354 1169
Bme1580I G_kGCm'C
Cuts at: 0 101 1184 1279 2113 2319 2510 2523
Size: 101 1083 95 834 206 191 13
Fragments arranged by size:
1083 834 206 191 101 95 13
BmrI ACTGGGnnnn_n'
Cuts at: 0 1484 2523
Size: 1484 1039
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 492 2523
Size: 492 2031
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 342 441 1414 1453 2523
Size: 280 62 99 973 39 1070
Fragments arranged by size:
1070 973 280 99 62 39
Bpu10I CC'TnA_GC
Cuts at: 0 1915 2512 2523
Size: 1915 597 11
Fragments arranged by size:
1915 597 11
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 832 1087 1780 2404 2523
Size: 832 255 693 624 119
Fragments arranged by size:
832 693 624 255 119
BsaAI yAC'GTr
Cuts at: 0 438 1482 2523
Size: 438 1044 1041
Fragments arranged by size:
1044 1041 438
BsaBI GATnn'nnATC
Cuts at: 0 187 2523
Size: 187 2336
BsaHI Gr'CG_yC
Cuts at: 0 452 601 1315 1766 2263 2523
Size: 452 149 714 451 497 260
Fragments arranged by size:
714 497 452 451 260 149
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 55 85 910 940 2523
Size: 55 30 825 30 1583
Fragments arranged by size:
1583 825 55 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1540 2523
Size: 1540 983
BseYI C'CCAG_C
Cuts at: 0 350 583 1953 2008 2112 2161 2179 2523
Size: 350 233 1370 55 104 49 18 344
Fragments arranged by size:
1370 350 344 233 104 55 49 18
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 202 394 418 736 783 1462 1504 1599
Size: 202 192 24 318 47 679 42 95
Cuts at: 1599 1734 2338 2376 2523
Size: 135 604 38 147
Fragments arranged by size:
679 604 318 202 192 147 135 95
47 42 38 24
BsiEI CG_ry'CG
Cuts at: 0 633 1058 1734 2523
Size: 633 425 676 789
Fragments arranged by size:
789 676 633 425
BsiHKAI G_wGCw'C
Cuts at: 0 82 206 239 361 1184 1364 2523
Size: 82 124 33 122 823 180 1159
Fragments arranged by size:
1159 823 180 124 122 82 33
BsmI GAATG_Cn'
Cuts at: 0 2152 2386 2523
Size: 2152 234 137
Fragments arranged by size:
2152 234 137
Bsp1286I G_dGCh'C
Cuts at: 0 82 101 206 239 361 1184 1279 1364
Size: 82 19 105 33 122 823 95 85
Cuts at: 1364 1726 2113 2319 2495 2510 2523
Size: 362 387 206 176 15 13
Fragments arranged by size:
823 387 362 206 176 122 105 95
85 82 33 19 15 13
BspHI T'CATG_A
Cuts at: 0 1784 2523
Size: 1784 739
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 16 158 224 262 582 867 1427 1548
Size: 16 142 66 38 320 285 560 121
Cuts at: 1548 1889 2351 2523
Size: 341 462 172
Fragments arranged by size:
560 462 341 320 285 172 142 121
66 38 16
BsrFI r'CCGG_y
Cuts at: 0 620 661 1184 1248 1450 1733 2523
Size: 620 41 523 64 202 283 790
Fragments arranged by size:
790 620 523 283 202 64 41
BssSI C'ACGA_G
Cuts at: 0 241 1522 2523
Size: 241 1281 1001
Fragments arranged by size:
1281 1001 241
BstAPI GCAn_nnn'nTGC
Cuts at: 0 86 1839 1851 2390 2399 2523
Size: 86 1753 12 539 9 124
Fragments arranged by size:
1753 539 124 86 12 9
BstEII G'GTnAC_C
Cuts at: 0 561 1446 1620 2523
Size: 561 885 174 903
Fragments arranged by size:
903 885 561 174
BstXI CCAn_nnnn'nTGG
Cuts at: 0 1041 2523
Size: 1041 1482
BstYI r'GATC_y
Cuts at: 0 773 971 1019 1574 1745 2090 2523
Size: 773 198 48 555 171 345 433
Fragments arranged by size:
773 555 433 345 198 171 48
BstZ17I GTA'TAC
Cuts at: 0 2027 2523
Size: 2027 496
BtgI C'CryG_G
Cuts at: 0 143 524 686 980 1058 1202 1223 1271
Size: 143 381 162 294 78 144 21 48
Cuts at: 1271 1344 1379 2372 2438 2523
Size: 73 35 993 66 85
Fragments arranged by size:
993 381 294 162 144 143 85 78
73 66 48 35 21
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 117 173 636 1644 2117 2523
Size: 117 56 463 1008 473 406
Fragments arranged by size:
1008 473 463 406 117 56
BtsI GCAGTG_nn'
Cuts at: 0 84 1192 1828 1861 2286 2458 2523
Size: 84 1108 636 33 425 172 65
Fragments arranged by size:
1108 636 425 172 84 65 33
DraIII CAC_nnn'GTG
Cuts at: 0 1179 2399 2523
Size: 1179 1220 124
Fragments arranged by size:
1220 1179 124
DrdI GACnn_nn'nnGTC
Cuts at: 0 1168 2523
Size: 1168 1355
EaeI y'GGCC_r
Cuts at: 0 16 630 644 683 1028 1055 1109 1205
Size: 16 614 14 39 345 27 54 96
Cuts at: 1205 1250 1580 1886 2030 2256 2523
Size: 45 330 306 144 226 267
Fragments arranged by size:
614 345 330 306 267 226 144 96
54 45 39 27 16 14
EagI C'GGCC_G
Cuts at: 0 630 1055 2523
Size: 630 425 1468
Fragments arranged by size:
1468 630 425
EarI CTCTTCn'nnn_
Cuts at: 0 1429 1657 2071 2523
Size: 1429 228 414 452
Fragments arranged by size:
1429 452 414 228
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 346 706 1032 1354 2059 2523
Size: 346 360 326 322 705 464
Fragments arranged by size:
705 464 360 346 326 322
EcoICRI GAG'CTC
Cuts at: 0 359 2523
Size: 359 2164
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 280 342 441 598 1414 1453 1699 2023
Size: 280 62 99 157 816 39 246 324
Cuts at: 2023 2523
Size: 500
Fragments arranged by size:
816 500 324 280 246 157 99 62
39
EcoNI CCTnn'n_nnAGG
Cuts at: 0 376 934 1555 2394 2523
Size: 376 558 621 839 129
Fragments arranged by size:
839 621 558 376 129
EcoO109I rG'GnC_Cy
Cuts at: 0 168 213 384 1276 1560 1995 2109 2315
Size: 168 45 171 892 284 435 114 206
Cuts at: 2315 2316 2523
Size: 1 207
Fragments arranged by size:
892 435 284 207 206 171 168 114
45 1
FspI TGC'GCA
Cuts at: 0 154 934 2523
Size: 154 780 1589
Fragments arranged by size:
1589 780 154
HaeII r_GCGC'y
Cuts at: 0 455 1066 1263 1411 1840 2137 2266 2366
Size: 455 611 197 148 429 297 129 100
Cuts at: 2366 2466 2523
Size: 100 57
Fragments arranged by size:
611 455 429 297 197 148 129 100
100 57
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 55 87 205 237 910 942 1036 1068
Size: 55 32 118 32 673 32 94 32
Cuts at: 1068 1933 1965 2041 2073 2082 2114 2523
Size: 865 32 76 32 9 32 409
Fragments arranged by size:
865 673 409 118 94 76 55 32
32 32 32 32 32 32 9
HincII GTy'rAC
Cuts at: 0 1770 2523
Size: 1770 753
KasI G'GCGC_C
Cuts at: 0 451 2262 2523
Size: 451 1811 261
Fragments arranged by size:
1811 451 261
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 851 2523
Size: 851 1672
MscI TGG'CCA
Cuts at: 0 685 1111 1582 1888 2523
Size: 685 426 471 306 635
Fragments arranged by size:
685 635 471 426 306
MslI CAynn'nnrTG
Cuts at: 0 416 679 2443 2523
Size: 416 263 1764 80
Fragments arranged by size:
1764 416 263 80
MspA1I CmG'CkG
Cuts at: 0 22 554 587 1225 1273 1392 1546 2012
Size: 22 532 33 638 48 119 154 466
Cuts at: 2012 2169 2523
Size: 157 354
Fragments arranged by size:
638 532 466 354 157 154 119 48
33 22
NaeI GCC'GGC
Cuts at: 0 622 1250 2523
Size: 622 628 1273
Fragments arranged by size:
1273 628 622
NarI GG'CG_CC
Cuts at: 0 452 2263 2523
Size: 452 1811 260
Fragments arranged by size:
1811 452 260
NcoI C'CATG_G
Cuts at: 0 143 524 1202 1379 2523
Size: 143 381 678 177 1144
Fragments arranged by size:
1144 678 381 177 143
NgoMIV G'CCGG_C
Cuts at: 0 620 1248 2523
Size: 620 628 1275
Fragments arranged by size:
1275 628 620
NotI GC'GGCC_GC
Cuts at: 0 630 2523
Size: 630 1893
NruI TCG'CGA
Cuts at: 0 2102 2523
Size: 2102 421
NspI r_CATG'y
Cuts at: 0 870 1962 2058 2523
Size: 870 1092 96 465
Fragments arranged by size:
1092 870 465 96
PasI CC'CwG_GG
Cuts at: 0 209 2523
Size: 209 2314
PciI A'CATG_T
Cuts at: 0 866 2523
Size: 866 1657
PflMI CCAn_nnn'nTGG
Cuts at: 0 192 1392 2087 2523
Size: 192 1200 695 436
Fragments arranged by size:
1200 695 436 192
PfoI T'CCnGG_A
Cuts at: 0 191 2523
Size: 191 2332
PmlI CAC'GTG
Cuts at: 0 438 1482 2523
Size: 438 1044 1041
Fragments arranged by size:
1044 1041 438
PpuMI rG'GwC_Cy
Cuts at: 0 168 213 384 1560 2523
Size: 168 45 171 1176 963
Fragments arranged by size:
1176 963 171 168 45
PspOMI G'GGCC_C
Cuts at: 0 1275 2109 2315 2523
Size: 1275 834 206 208
Fragments arranged by size:
1275 834 208 206
PstI C_TGCA'G
Cuts at: 0 1199 1556 1835 1844 2228 2252 2297 2523
Size: 1199 357 279 9 384 24 45 226
Fragments arranged by size:
1199 384 357 279 226 45 24 9
PvuII CAG'CTG
Cuts at: 0 2012 2169 2523
Size: 2012 157 354
Fragments arranged by size:
2012 354 157
SacI G_AGCT'C
Cuts at: 0 361 2523
Size: 361 2162
SacII CC_GC'GG
Cuts at: 0 1226 1274 2523
Size: 1226 48 1249
Fragments arranged by size:
1249 1226 48
SalI G'TCGA_C
Cuts at: 0 1768 2523
Size: 1768 755
SanDI GG'GwC_CC
Cuts at: 0 384 2523
Size: 384 2139
SapI GCTCTTCn'nnn_
Cuts at: 0 1429 1657 2071 2523
Size: 1429 228 414 452
Fragments arranged by size:
1429 452 414 228
ScaI AGT'ACT
Cuts at: 0 1438 2523
Size: 1438 1085
SexAI A'CCwGG_T
Cuts at: 0 2395 2523
Size: 2395 128
SfcI C'TryA_G
Cuts at: 0 1195 1215 1552 1831 1840 2224 2248 2293
Size: 1195 20 337 279 9 384 24 45
Cuts at: 2293 2523
Size: 230
Fragments arranged by size:
1195 384 337 279 230 45 24 20
9
SfiI GGCCn_nnn'nGGCC
Cuts at: 0 1588 2523
Size: 1588 935
SfoI GGC'GCC
Cuts at: 0 453 2264 2523
Size: 453 1811 259
Fragments arranged by size:
1811 453 259
SgrAI Cr'CCGG_yG
Cuts at: 0 661 2523
Size: 661 1862
SmaI CCC'GGG
Cuts at: 0 862 2523
Size: 862 1661
SmlI C'TyrA_G
Cuts at: 0 847 1102 1795 2419 2523
Size: 847 255 693 624 104
Fragments arranged by size:
847 693 624 255 104
SphI G_CATG'C
Cuts at: 0 1962 2523
Size: 1962 561
StuI AGG'CCT
Cuts at: 0 1236 1591 2389 2523
Size: 1236 355 798 134
Fragments arranged by size:
1236 798 355 134
StyI C'CwwG_G
Cuts at: 0 143 524 1202 1379 2358 2523
Size: 143 381 678 177 979 165
Fragments arranged by size:
979 678 381 177 165 143
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 97 856 1756 1848 2523
Size: 97 759 900 92 675
Fragments arranged by size:
900 759 675 97 92
TatI w'GTAC_w
Cuts at: 0 1436 1529 2523
Size: 1436 93 994
Fragments arranged by size:
1436 994 93
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 935 967 2523
Size: 935 32 1556
Fragments arranged by size:
1556 935 32
Tth111I GACn'n_nGTC
Cuts at: 0 1780 2523
Size: 1780 743
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 255 912 1038 1575 2208 2523
Size: 255 657 126 537 633 315
Fragments arranged by size:
657 633 537 315 255 126
XmaI C'CCGG_G
Cuts at: 0 860 2523
Size: 860 1663
ZraI GAC'GTC
Cuts at: 0 602 1316 1767 2523
Size: 602 714 451 756
Fragments arranged by size:
756 714 602 451
Enzymes that do cut:
AarI AatII AccI AcuI AfeI AflIII AhdI AleI
AlfI AlwNI ApaI ApaLI ApoI AvaI BamHI BanI
BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI
BglII BlpI Bme1580I BmrI BplI BpmI Bpu10I BpuEI
BsaAI BsaBI BsaHI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI Bsp1286I BspHI BspMI BsrFI BssSI BstAPI
BstEII BstXI BstYI BstZ17I BtgI BtgZI BtsI DraIII
DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI
EcoO109I FspI HaeII Hin4I HincII KasI MmeI MscI
MslI MspA1I NaeI NarI NcoI NgoMIV NotI NruI
NspI PasI PciI PflMI PfoI PmlI PpuMI PspOMI
PstI PvuII SacI SacII SalI SanDI SapI ScaI
SexAI SfcI SfiI SfoI SgrAI SmaI SmlI SphI
StuI StyI TaqII TatI TstI Tth111I XcmI XmaI
ZraI
Enzymes that do not cut:
Acc65I AclI AflII AgeI AloI AscI AseI AsiSI
AvrII BaeI BfrBI BmgBI BmtI BsaI BsaWI BsiWI
BsmBI BspEI BsrBI BsrDI BsrGI BssHII BstBI Bsu36I
ClaI CspCI DraI EcoRI EcoRV FalI FseI FspAI
HindIII HpaI KpnI MfeI MluI NdeI NheI NsiI
PacI PmeI PpiI PshAI PsiI PspXI PsrI PvuI
RsrII SbfI SgfI SnaBI SpeI SrfI SspI SwaI
XbaI XhoI XmnI