Restriction map for
pFN21AB8727
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# NO CUT ENZYMES ( 105 enzymes) #
##################################
AatII Acc65I AccIII AclI AfaI
AflII AgeI Alw44I Aor51HI ApaLI
AscI AseI AsnI Asp718I AspI
AvrII BamHI BbeI BcgI BciVI
BclI BfaI BfrI BlnI BlpI
Bpu1102I BsaBI BsaI BseAI BsgI
BsiEI BsiWI BsmI Bsp1407I BspDI
BspEI BspHI BsrBI BsrDI BsrGI
BssHII Bst1107I BstAPI BstBI BstZ17I
CelII ClaI DraI DrdI EagI
EclXI Eco47III Eco52I EcoRI EcoRV
EcoT22I FbaI FseI HindIII HpaI
KasI KpnI MaeI MamI MfeI
MroI MseI MunI NarI NcoI
NdeI NheI NotI NruI NsiI
NspV PacI PaeR7I PflFI PinAI
PmeI PshAI PshBI Psp1406I PvuI
RcaI RsaI SbfI ScaI SexAI
SfiI SfoI SfuI SgrAI SmlI
SnaBI SpeI Sse8387I SspBI SspI
SwaI Tru9I Tth111I XbaI XhoI
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# ONE CUT ENZYMES ( 55 enzymes) #
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AccI 1079 GTCGAC GT^[AC][GT]AC
AcyI 1198 GACGCC G[GA]^CG[CT]C
AhdI 469 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AlwI 1224 GATCC GGATC(4/5)
AocI 1303 CCTAAGG CC^T[ACGT]AGG
Asp700I 18 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
AspEI 469 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
AvaI 1404 CCCGGG C^[CT]CG[GA]G
AviII 659 TGCGCA TGC^GCA
BbsI 1437 GAAGAC GAAGAC(2/6)
BglI 946 GCCGAGCCGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 104 AGATCT A^GATCT
BpuAI 1437 GAAGAC GAAGAC(2/6)
BsaHI 1198 GACGCC G[GA]^CG[CT]C
BsaWI 613 TCCGGT [AT]^CCGG[AT]
BseRI 844 GAGGAG GAGGAG(10/8)
BsmBI 124 GAGACG CGTCTC(1/5)
BsoBI 1404 CCCGGG C^[CT]CG[GA]G
BspLU11I 1100 ACATGT A^CATGT
BspMI 428 ACCTGC ACCTGC(4/8)
BssSI 193 CACGAG CACGAG(-5/-1)
BstXI 1274 CCACTGACGTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
BstYI 104 AGATCT [GA]^GATC[CT]
Bsu36I 1303 CCTAAGG CC^T[ACGT]AGG
CpoI 1361 CGGACCG CG^G[AT]CCG
DraIII 193 CACGAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 1358 CCGCGG C^C[GA][CT]GG
Eam1105I 469 GACATGCTGTC GAC[ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
Eco81I 1303 CCTAAGG CC^T[ACGT]AGG
EcoNI 1245 CCTCATGGAGG CCT[ACGT][ACGT]^[ACGT][ACGT][ACGT]AGG
EcoT14I 929 CCAAGG C^C[AT][AT]GG
FspI 659 TGCGCA TGC^GCA
HaeII 1425 AGCGCC [GA]GCGC^[CT]
Hin1I 1198 GACGCC G[GA]^CG[CT]C
HincII 1079 GTCGAC GT[CT]^[GA]AC
HindII 1079 GTCGAC GT[CT]^[GA]AC
KspI 1358 CCGCGG CCGC^GG
MflI 104 AGATCT [GA]^GATC[CT]
MluI 377 ACGCGT A^CGCGT
NaeI 951 GCCGGC GCC^GGC
NgoMIV 951 GCCGGC G^CCGGC
PflMI 255 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
RsrII 1361 CGGACCG CG^G[AT]CCG
SacII 1358 CCGCGG CCGC^GG
SalI 1079 GTCGAC G^TCGAC
SapI 1297 GAAGAGC GCTCTTC(1/4)
SmaI 1404 CCCGGG CCC^GGG
SphI 579 GCATGC GCATG^C
StyI 929 CCAAGG C^C[AT][AT]GG
Van91I 255 CCACGTGCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XcmI 455 CCATGACTGACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
XhoII 104 AGATCT [GA]^GATC[CT]
XmaCI 1404 CCCGGG C^CCGGG
XmaI 1404 CCCGGG C^CCGGG
XmnI 18 GAACCCGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
###################################
# TWO CUT ENZYMES ( 27 enzymes) #
###################################
AcsI 1324 GAATTT [GA]^AATT[CT]
1333 AAATTC
AflIII 377 ACGCGT A^C[GA][CT]GT
1100 ACATGT
AlwNI 1060 CAGTCTCTG CAG[ACGT][ACGT][ACGT]^CTG
1264 CAGTGGCTG
ApoI 1324 GAATTT [GA]^AATT[CT]
1333 AAATTC
BalI 266 TGGCCA TGG^CCA
573 TGGCCA
BanI 245 GGCACC G^G[CT][GA]CC
723 GGTGCC
BbrPI 256 CACGTG CAC^GTG
777 CACGTG
BbvCI 1388 CCTCAGC CCTCAGC(-5/-2)
1393 GCTGAGG
BsaAI 256 CACGTG [CT]AC^GT[GA]
777 CACGTG
BsrFI 951 GCCGGC [GA]^CCGG[CT]
994 ACCGGC
BstEII 411 GGTCACC G^GT[ACGT]ACC
1311 GGTCACC
BstPI 411 GGTCACC G^GT[ACGT]ACC
1311 GGTCACC
Cfr10I 951 GCCGGC [GA]^CCGG[CT]
994 ACCGGC
EaeI 266 TGGCCA [CT]^GGCC[GA]
573 TGGCCA
EarI 165 GAAGAG CTCTTC(1/4)
1297 GAAGAG
EcoO65I 411 GGTCACC G^GT[ACGT]ACC
1311 GGTCACC
HgaI 376 GACGC GACGC(5/10)
1198 GACGC
Ksp632I 165 GAAGAG CTCTTC(1/4)
1297 GAAGAG
MluNI 266 TGGCCA TGG^CCA
573 TGGCCA
MscI 266 TGGCCA TGG^CCA
573 TGGCCA
MslI 330 CACCATCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
1275 CACTGACGTG
PleI 550 GACTC GAGTC(4/5)
568 GACTC
PmaCI 256 CACGTG CAC^GTG
777 CACGTG
PmlI 256 CACGTG CAC^GTG
777 CACGTG
SacI 766 GAGCTC GAGCT^C
808 GAGCTC
TfiI 170 GAATC G^A[AT]TC
535 GATTC
Tsp509I 1325 AATT ^AATT
1334 AATT
#########################################
# 3 OR MORE CUT ENZYMES ( 71 enzymes) #
#########################################
AccII AciI AluI ApaI AspHI
AvaII BanII BbvI BcnI BmyI
BpmI BsaJI BsiHKAI BsiYI BslI
BsmAI BsmFI Bsp1286I BsrI BssKI
BstF5I BstNI BstUI Cac8I CfoI
Cfr13I DdeI DpnII DraII EcoO109I
EcoRII FauI Fnu4HI FokI HaeIII
HapII HhaI HinP1I HinfI HpaII
HphI ItaI MaeII MaeIII MboI
MboII MnlI MspA1I MspI MvaI
MvnI MwoI NciI NdeII NlaIII
NlaIV NspI PpuMI PstI PvuII
Sau3AI Sau96I ScrFI SfaNI SfcI
StuI TaqI TseI Tsp45I TspRI
TthHB8I