(Circular) (Six-base) MAPSORT of: pFN21AB8727.seq Check: 7488 from: 1 to: 6181 pFN21AB8727 6181 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3991 278 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3085 3524 1937 Size: 1148 439 4594 Fragments arranged by size: 4594 1148 439 Acc65I G'GTAC_C Cuts at: 3502 3502 Size: 6181 AclI AA'CG_TT Cuts at: 1563 4308 4681 1563 Size: 2745 373 3063 Fragments arranged by size: 3063 2745 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4244 5628 5896 1632 Size: 2612 1384 268 1917 Fragments arranged by size: 2612 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5984 AflIII A'CryG_T Cuts at: 1203 2382 3105 5080 6057 1203 Size: 1179 723 1975 977 1327 Fragments arranged by size: 1975 1327 1179 977 723 AgeI A'CCGG_T Cuts at: 5012 5012 Size: 6181 AhdI GACnn_n'nnGTC Cuts at: 2479 4910 2479 Size: 2431 3750 AleI CACnn'nnGTG Cuts at: 1166 2339 3284 1166 Size: 1173 945 4063 Fragments arranged by size: 4063 1173 945 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6181 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3023 3055 3023 Size: 32 6149 AlwNI CAG_nnn'CTG Cuts at: 3070 3274 3924 5496 3070 Size: 204 650 1572 3755 Fragments arranged by size: 3755 1572 650 204 ApaI G_GGCC'C Cuts at: 2098 2984 3407 2098 Size: 886 423 4872 Fragments arranged by size: 4872 886 423 ApaLI G'TGCA_C Cuts at: 4238 5394 4238 Size: 1156 5025 ApoI r'AATT_y Cuts at: 1493 3329 3338 3490 3716 3868 5977 1493 Size: 1836 9 152 226 152 2109 1697 Fragments arranged by size: 2109 1836 1697 226 152 152 9 AseI AT'TA_AT Cuts at: 160 1032 4735 160 Size: 872 3703 1606 Fragments arranged by size: 3703 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 AvaI C'yCGr_G Cuts at: 1870 1943 3409 3506 1870 Size: 73 1466 97 4545 Fragments arranged by size: 4545 1466 97 73 BamHI G'GATC_C Cuts at: 3511 3878 3511 Size: 367 5814 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2250 2728 3502 4957 618 Size: 325 217 627 463 478 774 1455 1842 Fragments arranged by size: 1842 1455 774 627 478 463 325 217 BanII G_rGCy'C Cuts at: 1391 2098 2748 2775 2817 2984 3041 3199 3407 Size: 707 650 27 42 167 57 158 208 Cuts at: 3407 3500 1391 Size: 93 4072 Fragments arranged by size: 4072 707 650 208 167 158 93 57 42 27 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3449 928 Size: 971 1550 3660 Fragments arranged by size: 3660 1550 971 BbvCI CC'TCA_GC Cuts at: 3394 3399 3394 Size: 5 6176 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4372 4406 1903 Size: 34 2435 34 3678 Fragments arranged by size: 3678 2435 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4073 5283 4073 Size: 1210 4971 BclI T'GATC_A Cuts at: 1548 1548 Size: 6181 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2957 4792 136 Size: 107 122 71 631 605 1285 1835 1525 Fragments arranged by size: 1835 1525 1285 631 605 122 107 71 BglII A'GATC_T Cuts at: 2109 6176 2109 Size: 4067 2114 BlpI GC'TnA_GC Cuts at: 3598 3598 Size: 6181 Bme1580I G_kGCm'C Cuts at: 1790 2098 2253 2552 2733 2984 3407 4242 5398 Size: 308 155 299 181 251 423 835 1156 Cuts at: 5398 1790 Size: 2573 Fragments arranged by size: 2573 1156 835 423 308 299 251 181 155 BmgBI CAC'GTC Cuts at: 1318 3286 1318 Size: 1968 4213 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2101 3227 3912 Size: 416 53 83 399 24 684 1126 685 Cuts at: 3912 4870 442 Size: 958 1753 Fragments arranged by size: 1753 1126 958 685 684 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6181 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2022 2471 2542 2696 2777 2831 3025 3092 Size: 783 449 71 154 81 54 194 67 Cuts at: 3092 3213 3370 4841 1239 Size: 121 157 1471 2579 Fragments arranged by size: 2579 1471 783 449 194 157 154 121 81 71 67 54 Bpu10I CC'TnA_GC Cuts at: 3394 3399 3394 Size: 5 6176 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4306 5171 5469 4306 Size: 865 298 5018 Fragments arranged by size: 5018 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4844 882 Size: 617 3345 2219 Fragments arranged by size: 3345 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2263 2784 3972 493 Size: 711 1059 521 1188 2702 Fragments arranged by size: 2702 1188 1059 711 521 BsaBI GATnn'nnATC Cuts at: 6175 6175 Size: 6181 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3204 3988 4370 5005 Size: 53 83 186 936 1671 784 382 635 Cuts at: 5005 275 Size: 1451 Fragments arranged by size: 1671 1451 936 784 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2618 4614 5012 5286 5433 5844 2618 Size: 1996 398 274 147 411 2955 Fragments arranged by size: 2955 1996 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3023 3053 1178 Size: 30 165 30 1620 30 4306 Fragments arranged by size: 4306 1620 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2864 1186 Size: 1678 4503 BseYI C'CCAG_C Cuts at: 2989 3015 3427 5384 5880 2989 Size: 26 412 1957 496 3290 Fragments arranged by size: 3290 1957 496 412 26 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5608 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3631 4392 5420 1540 Size: 275 186 1630 761 1028 2301 Fragments arranged by size: 2301 1630 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2358 2775 2817 3221 4242 4327 5398 2358 Size: 417 42 404 1021 85 1071 3141 Fragments arranged by size: 3141 1071 1021 417 404 85 42 BsmI GAATG_Cn' Cuts at: 3692 3785 3692 Size: 93 6088 BsmBI CGTCTCn'nnnn_ Cuts at: 2123 2123 Size: 6181 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2098 2253 2358 2552 2733 2748 2775 Size: 399 308 155 105 194 181 15 27 Cuts at: 2775 2817 2984 3041 3199 3221 3407 3500 4242 Size: 42 167 57 158 22 186 93 742 Cuts at: 4242 4327 5398 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 308 194 186 181 167 158 155 105 93 85 57 42 27 22 15 BspEI T'CCGG_A Cuts at: 5844 5844 Size: 6181 BspHI T'CATG_A Cuts at: 4070 5800 4070 Size: 1730 4451 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2442 3536 844 Size: 1598 1094 3489 Fragments arranged by size: 3489 1598 1094 BsrBI CCG'CTC Cuts at: 1394 1823 4068 1394 Size: 429 2245 3507 Fragments arranged by size: 3507 2245 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4676 4850 65 Size: 1183 3428 174 1396 Fragments arranged by size: 3428 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2956 2999 4825 5012 1700 Size: 110 1146 43 1826 187 2869 Fragments arranged by size: 2869 1826 1146 187 110 43 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6181 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6181 BssSI C'ACGA_G Cuts at: 2198 4241 5253 2198 Size: 2043 1012 3126 Fragments arranged by size: 3126 2043 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6181 BstBI TT'CG_AA Cuts at: 4985 4985 Size: 6181 BstEII G'GTnAC_C Cuts at: 1184 2416 3316 1184 Size: 1232 900 4049 Fragments arranged by size: 4049 1232 900 BstXI CCAn_nnnn'nTGG Cuts at: 3286 3286 Size: 6181 BstYI r'GATC_y Cuts at: 1972 2109 3511 3878 4263 4280 5732 5818 5830 Size: 137 1402 367 385 17 1452 86 12 Cuts at: 5830 6176 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1402 385 367 346 137 86 17 12 Bsu36I CC'TnA_GG Cuts at: 3309 3309 Size: 6181 BtgI C'CryG_G Cuts at: 513 1065 2004 3363 513 Size: 552 939 1359 3331 Fragments arranged by size: 3331 1359 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5442 Fragments arranged by size: 5442 683 56 BtsI GCAGTG_nn' Cuts at: 2674 3052 3275 3697 4491 4511 2674 Size: 378 223 422 794 20 4344 Fragments arranged by size: 4344 794 422 378 223 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6181 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6146 DraI TTT'AAA Cuts at: 3485 3485 Size: 6181 DraIII CAC_nnn'GTG Cuts at: 2203 2203 Size: 6181 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5188 809 Size: 507 3872 1802 Fragments arranged by size: 3872 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2271 2578 3628 Size: 54 1425 50 275 18 441 307 1050 Cuts at: 3628 4517 6146 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 1050 889 441 307 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3628 1537 Size: 275 1816 4090 Fragments arranged by size: 4090 1816 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 3297 4111 1353 Size: 812 1132 814 3423 Fragments arranged by size: 3423 1132 814 812 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4768 5142 5288 387 Size: 1064 138 3179 374 146 1280 Fragments arranged by size: 3179 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2773 2815 2773 Size: 42 6139 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2022 2471 2542 2696 2777 2831 3025 Size: 393 390 449 71 154 81 54 194 Cuts at: 3025 3092 3213 3370 4244 4841 5628 5896 1239 Size: 67 121 157 874 597 787 268 1524 Fragments arranged by size: 1524 874 787 597 449 393 390 268 194 157 154 121 81 71 67 54 EcoNI CCTnn'n_nnAGG Cuts at: 3254 3254 Size: 6181 EcoO109I rG'GnC_Cy Cuts at: 2095 2141 2431 2806 2938 2981 3403 3404 3413 Size: 46 290 375 132 43 422 1 9 Cuts at: 3413 2095 Size: 4863 Fragments arranged by size: 4863 422 375 290 132 46 43 9 1 EcoRI G'AATT_C Cuts at: 3490 3868 3490 Size: 378 5803 FspI TGC'GCA Cuts at: 2666 4687 2666 Size: 2021 4160 HaeII r_GCGC'y Cuts at: 1678 3434 5328 1678 Size: 1756 1894 2531 Fragments arranged by size: 2531 1894 1756 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2037 2069 2197 2229 2465 Size: 32 135 32 465 32 128 32 236 Cuts at: 2465 2497 2513 2545 4822 4854 4896 4928 1373 Size: 32 16 32 2277 32 42 32 2626 Fragments arranged by size: 2626 2277 465 236 135 128 42 32 32 32 32 32 32 32 32 16 HincII GTy'rAC Cuts at: 669 1657 1938 3086 3525 3771 669 Size: 988 281 1148 439 246 3079 Fragments arranged by size: 3079 1148 988 439 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6181 HpaI GTT'AAC Cuts at: 3771 3771 Size: 6181 KpnI G_GTAC'C Cuts at: 3506 3506 Size: 6181 MfeI C'AATT_G Cuts at: 3780 3780 Size: 6181 MluI A'CGCG_T Cuts at: 2382 6057 2382 Size: 3675 2506 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2688 5295 5479 1305 Size: 1383 2607 184 2007 Fragments arranged by size: 2607 2007 1383 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 2580 10 Size: 54 1425 343 441 307 3611 Fragments arranged by size: 3611 1425 441 343 307 54 MslI CAynn'nnrTG Cuts at: 518 1166 2339 3284 4139 4498 4657 518 Size: 648 1173 945 855 359 159 2042 Fragments arranged by size: 2042 1173 945 855 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2803 2842 2963 3019 3365 3398 3597 4274 Size: 1197 39 121 56 346 33 199 677 Cuts at: 4274 5422 5667 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1197 1148 677 346 245 199 121 56 39 33 NaeI GCC'GGC Cuts at: 2958 2958 Size: 6181 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4690 Fragments arranged by size: 4690 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6181 NgoMIV G'CCGG_C Cuts at: 2956 2956 Size: 6181 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6181 NotI GC'GGCC_GC Cuts at: 3628 3628 Size: 6181 NspI r_CATG'y Cuts at: 1132 1229 2479 2588 3109 3469 3539 5084 1132 Size: 97 1250 109 521 360 70 1545 2229 Fragments arranged by size: 2229 1545 1250 521 360 109 97 70 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6181 PciI A'CATG_T Cuts at: 3105 5080 3105 Size: 1975 4206 PflMI CCAn_nnn'nTGG Cuts at: 1108 2266 1108 Size: 1158 5023 PfoI T'CCnGG_A Cuts at: 2808 3342 2808 Size: 534 5647 PmeI GTTT'AAAC Cuts at: 3485 3485 Size: 6181 PmlI CAC'GTG Cuts at: 2263 2784 2263 Size: 521 5660 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3023 3055 4250 4282 5763 5795 3023 Size: 32 1195 32 1481 32 3409 Fragments arranged by size: 3409 1481 1195 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2141 2431 2806 3413 2141 Size: 290 375 607 4909 Fragments arranged by size: 4909 607 375 290 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6181 PsiI TTA'TAA Cuts at: 3751 3751 Size: 6181 PspOMI G'GGCC_C Cuts at: 2094 2980 3403 2094 Size: 886 423 4872 Fragments arranged by size: 4872 886 423 PstI C_TGCA'G Cuts at: 830 2082 2769 3066 3533 830 Size: 1252 687 297 467 3478 Fragments arranged by size: 3478 1252 687 467 297 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6181 PvuII CAG'CTG Cuts at: 2842 2963 3019 3398 2842 Size: 121 56 379 5625 Fragments arranged by size: 5625 379 121 56 RsrII CG'GwC_CG Cuts at: 3367 3367 Size: 6181 SacI G_AGCT'C Cuts at: 2775 2817 2775 Size: 42 6139 SacII CC_GC'GG Cuts at: 3366 3366 Size: 6181 SalI G'TCGA_C Cuts at: 1936 3084 3523 1936 Size: 1148 439 4594 Fragments arranged by size: 4594 1148 439 SanDI GG'GwC_CC Cuts at: 3413 3413 Size: 6181 SapI GCTCTTCn'nnn_ Cuts at: 3297 3297 Size: 6181 SbfI CC_TGCA'GG Cuts at: 3533 3533 Size: 6181 ScaI AGT'ACT Cuts at: 4429 6141 4429 Size: 1712 4469 SfcI C'TryA_G Cuts at: 826 1045 2078 2765 3062 3529 4664 5345 5536 Size: 219 1033 687 297 467 1135 681 191 Cuts at: 5536 826 Size: 1471 Fragments arranged by size: 1471 1135 1033 687 681 467 297 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6181 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6181 SmaI CCC'GGG Cuts at: 1872 3411 3508 1872 Size: 1539 97 4545 Fragments arranged by size: 4545 1539 97 SmlI C'TyrA_G Cuts at: 820 1017 1943 4285 5186 5448 820 Size: 197 926 2342 901 262 1553 Fragments arranged by size: 2342 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3972 493 Size: 3479 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6181 SphI G_CATG'C Cuts at: 1132 2588 3539 1132 Size: 1456 951 3774 Fragments arranged by size: 3774 1456 951 SspI AAT'ATT Cuts at: 5 52 4105 5 Size: 47 4053 2081 Fragments arranged by size: 4053 2081 47 StuI AGG'CCT Cuts at: 1517 2448 3077 3245 1517 Size: 931 629 168 4453 Fragments arranged by size: 4453 931 629 168 StyI C'CwwG_G Cuts at: 513 1065 2004 2934 3620 513 Size: 552 939 930 686 3074 Fragments arranged by size: 3074 939 930 686 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2347 2500 2750 3254 3384 4208 4225 Size: 1255 767 153 250 504 130 824 17 Cuts at: 4225 4378 4563 5008 325 Size: 153 185 445 1498 Fragments arranged by size: 1498 1255 824 767 504 445 250 185 153 153 130 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4427 6139 96 Size: 274 80 33 51 1443 2450 1712 138 Fragments arranged by size: 2450 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6141 6173 6141 Size: 32 6149 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6181 XbaI T'CTAG_A Cuts at: 3517 3517 Size: 6181 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2467 2467 Size: 6181 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6181 XmaI C'CCGG_G Cuts at: 1870 3409 3506 1870 Size: 1539 97 4545 Fragments arranged by size: 4545 1539 97 XmnI GAAnn'nnTTC Cuts at: 2027 4310 2027 Size: 2283 3898 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3989 276 Size: 53 83 186 936 2455 2468 Fragments arranged by size: 2468 2455 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII RsrII SacI SacII SalI SanDI SapI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BstZ17I EcoRV FalI FseI FspAI KasI NarI NruI NsiI PacI PspXI PsrI SexAI SfiI SfoI SrfI SwaI