Restriction map for
pFN21AB8726
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# NO CUT ENZYMES ( 123 enzymes) #
##################################
Acc65I AccI AclI AcsI AflII
AflIII AgeI AhdI Alw44I AlwNI
ApaI ApaLI ApoI AscI AseI
AsnI Asp700I Asp718I AspEI AviII
AvrII BalI BamHI BbeI BbrPI
BcgI BclI BfaI BfrI BglI
BlnI BlpI Bpu1102I BsaAI BsgI
BsiWI BsmBI BsmI BspHI BspLU11I
BsrBI BssHII Bst1107I BstBI BstEII
BstPI BstZ17I CelII CpoI DraI
DrdI EagI Eam1105I EarI EclXI
Eco52I EcoO65I EcoRI EcoRV EcoT22I
FbaI FseI FspI HpaI HphI
KasI KpnI Ksp632I KspI MaeI
MfeI MluI MluNI MscI MseI
MunI NaeI NarI NcoI NgoMIV
NheI NotI NruI NsiI NspV
PacI PinAI PmaCI PmeI PmlI
PshAI PshBI Psp1406I PvuI PvuII
RcaI RsrII SacI SacII SalI
SapI SbfI SexAI SfiI SfoI
SfuI SgrAI SmaI SnaBI SpeI
Sse8387I SspI StuI SwaI Tru9I
Tsp45I Tsp509I TspRI XbaI XcmI
XmaCI XmaI XmnI
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# ONE CUT ENZYMES ( 56 enzymes) #
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AatII 766 GACGTC GACGT^C
AccIII 1045 TCCGGA T^CCGGA
AocI 18 CCTAAGG CC^T[ACGT]AGG
Aor51HI 476 AGCGCT AGC^GCT
AspHI 688 GAGCAC G[AT]GC[AT]^C
AspI 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
BanI 975 GGTGCC G^G[CT][GA]CC
BanII 317 GAGCCC G[GA]GC[CT]^C
BbsI 840 GTCTTC GAAGAC(2/6)
BciVI 677 GTATCC GTATCC(6/5)
BcnI 402 CCGGG CC^[GC]GG
BglII 230 AGATCT A^GATCT
BpuAI 840 GTCTTC GAAGAC(2/6)
BsaWI 1045 TCCGGA [AT]^CCGG[AT]
BseAI 1045 TCCGGA T^CCGGA
BsiEI 129 CGACCG CG[GA][CT]^CG
BsiHKAI 688 GAGCAC G[AT]GC[AT]^C
Bsp1407I 617 TGTACA T^GTACA
BspDI 787 ATCGAT AT^CGAT
BspEI 1045 TCCGGA T^CCGGA
BspMI 724 ACCTGC ACCTGC(4/8)
BsrDI 494 GCAATG GCAATG(2/0)
BsrGI 617 TGTACA T^GTACA
BsrI 894 ACTGG ACTGG(1/-1)
BssSI 807 CTCGTG CACGAG(-5/-1)
BstXI 936 CCACAAGCTTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
Bsu36I 18 CCTAAGG CC^T[ACGT]AGG
ClaI 787 ATCGAT AT^CGAT
DraIII 691 CACCGTGTG CAC[ACGT][ACGT][ACGT]^GTG
DsaI 204 CCACGG C^C[GA][CT]GG
EaeI 1010 TGGCCG [CT]^GGCC[GA]
Eco47III 476 AGCGCT AGC^GCT
Eco81I 18 CCTAAGG CC^T[ACGT]AGG
EcoT14I 581 CCAAGG C^C[AT][AT]GG
HgaI 278 GCGTC GACGC(5/10)
HincII 199 GTCAAC GT[CT]^[GA]AC
HindII 199 GTCAAC GT[CT]^[GA]AC
HindIII 940 AAGCTT A^AGCTT
MaeIII 32 GTTAC ^GT[ACGT]AC
MroI 1045 TCCGGA T^CCGGA
MspA1I 775 CAGCGG C[AC]G^C[GT]G
NciI 402 CCGGG CC^[GC]GG
NdeI 539 CATATG CA^TATG
NspI 752 GCATGC [GA]CATG^[CT]
PaeR7I 295 CTCGAG C^TCGAG
PflFI 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
PflMI 381 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
PleI 652 GACTC GAGTC(4/5)
ScaI 374 AGTACT AGT^ACT
SphI 752 GCATGC GCATG^C
SspBI 617 TGTACA T^GTACA
StyI 581 CCAAGG C^C[AT][AT]GG
TfiI 164 GATTC G^A[AT]TC
Tth111I 391 GACATCGTC GAC[ACGT]^[ACGT][ACGT]GTC
Van91I 381 CCACGACCTGG CCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGG
XhoI 295 CTCGAG C^TCGAG
###################################
# TWO CUT ENZYMES ( 22 enzymes) #
###################################
AccII 707 CGCG CG^CG
880 CGCG
AcyI 277 GGCGTC G[GA]^CG[CT]C
766 GACGTC
AvaII 405 GGACC G^G[AT]CC
738 GGACC
BpmI 1020 CTCCAG CTGGAG(16/14)
597 CTGGAG
BsaBI 180 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
255 GATCTACATC
BsaHI 277 GGCGTC G[GA]^CG[CT]C
766 GACGTC
BsaI 238 GAGACC GGTCTC(1/5)
1015 GAGACC
BsmAI 238 GAGAC GTCTC(1/5)
1015 GAGAC
BsmFI 404 GGGAC GGGAC(10/14)
488 GGGAC
BsrFI 926 ACCGGC [GA]^CCGG[CT]
962 ACCGGC
BstAPI 535 GCAGCATATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
960 GCACCGGCTGC
BstUI 707 CGCG CG^CG
880 CGCG
Cfr10I 926 ACCGGC [GA]^CCGG[CT]
962 ACCGGC
DraII 404 GGGACCT [GA]G^G[ACGT]CC[CT]
737 AGGACCT
EcoO109I 404 GGGACCT [GA]G^G[ACGT]CC[CT]
737 AGGACCT
HaeII 95 AGCGCC [GA]GCGC^[CT]
476 AGCGCT
Hin1I 277 GGCGTC G[GA]^CG[CT]C
766 GACGTC
HinfI 164 GATTC G^A[ACGT]TC
652 GACTC
MamI 180 GATGGACATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
255 GATCTACATC
MslI 261 CATCATCATG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
784 CACATCGATG
MvnI 707 CGCG CG^CG
880 CGCG
PpuMI 404 GGGACCT [GA]G^G[AT]CC[CT]
737 AGGACCT
#########################################
# 3 OR MORE CUT ENZYMES ( 57 enzymes) #
#########################################
AciI AfaI AluI AlwI AvaI
BbvCI BbvI BmyI BsaJI BseRI
BsiYI BslI BsoBI Bsp1286I BssKI
BstF5I BstNI BstYI Cac8I CfoI
Cfr13I DdeI DpnII EcoNI EcoRII
FauI Fnu4HI FokI HaeIII HapII
HhaI HinP1I HpaII ItaI MaeII
MboI MboII MflI MnlI MspI
MvaI MwoI NdeII NlaIII NlaIV
PstI RsaI Sau3AI Sau96I ScrFI
SfaNI SfcI SmlI TaqI TseI
TthHB8I XhoII