(Circular) (Six-base) MAPSORT of: pFN21AB8726.seq Check: 2543 from: 1 to: 5779 pFN21AB8726 5779 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 2775 3589 278 Size: 53 83 186 936 1239 814 2468 Fragments arranged by size: 2468 1239 936 814 186 83 53 AccI GT'mk_AC Cuts at: 1937 3122 1937 Size: 1185 4594 Acc65I G'GTAC_C Cuts at: 3100 3100 Size: 5779 AclI AA'CG_TT Cuts at: 1563 3906 4279 1563 Size: 2343 373 3063 Fragments arranged by size: 3063 2343 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3842 5226 5494 1632 Size: 2210 1384 268 1917 Fragments arranged by size: 2210 1917 1384 268 AfeI AGC'GCT Cuts at: 2483 2483 Size: 5779 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5582 AflIII A'CryG_T Cuts at: 1203 4678 5655 1203 Size: 3475 977 1327 Fragments arranged by size: 3475 1327 977 AgeI A'CCGG_T Cuts at: 4610 4610 Size: 5779 AhdI GACnn_n'nnGTC Cuts at: 4508 4508 Size: 5779 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5779 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5779 AlwNI CAG_nnn'CTG Cuts at: 3522 5094 3522 Size: 1572 4207 ApaLI G'TGCA_C Cuts at: 3836 4992 3836 Size: 1156 4623 ApoI r'AATT_y Cuts at: 1493 3088 3314 3466 5575 1493 Size: 1595 226 152 2109 1697 Fragments arranged by size: 2109 1697 1595 226 152 AseI AT'TA_AT Cuts at: 160 1032 4333 160 Size: 872 3301 1606 Fragments arranged by size: 3301 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 AvaI C'yCGr_G Cuts at: 1870 1943 2178 2300 2555 2984 3104 1870 Size: 73 235 122 255 429 120 4545 Fragments arranged by size: 4545 429 255 235 122 120 73 BamHI G'GATC_C Cuts at: 3109 3476 3109 Size: 367 5412 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2980 3100 4555 618 Size: 325 217 627 1193 120 1455 1842 Fragments arranged by size: 1842 1455 1193 627 325 217 120 BanII G_rGCy'C Cuts at: 1391 2326 3098 1391 Size: 935 772 4072 Fragments arranged by size: 4072 935 772 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2838 928 Size: 971 939 3869 Fragments arranged by size: 3869 971 939 BbvCI CC'TCA_GC Cuts at: 2516 2804 3057 2516 Size: 288 253 5238 Fragments arranged by size: 5238 288 253 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 3970 4004 1903 Size: 34 2033 34 3678 Fragments arranged by size: 3678 2033 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2693 3671 4881 2693 Size: 978 1210 3591 Fragments arranged by size: 3591 1210 978 BclI T'GATC_A Cuts at: 1548 1548 Size: 5779 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4390 136 Size: 107 122 71 631 605 2718 1525 Fragments arranged by size: 2718 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 2235 5774 2235 Size: 3539 2240 BlpI GC'TnA_GC Cuts at: 3196 3196 Size: 5779 Bme1580I G_kGCm'C Cuts at: 1790 2985 3840 4996 1790 Size: 1195 855 1156 2573 Fragments arranged by size: 2573 1195 1156 855 BmgBI CAC'GTC Cuts at: 1318 2338 2844 1318 Size: 1020 506 4253 Fragments arranged by size: 4253 1020 506 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3510 4468 442 Size: 416 53 83 399 24 2093 958 1753 Fragments arranged by size: 2093 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5779 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2623 3010 4439 1239 Size: 1384 387 1429 2579 Fragments arranged by size: 2579 1429 1384 387 Bpu10I CC'TnA_GC Cuts at: 2516 2804 3057 2516 Size: 288 253 5238 Fragments arranged by size: 5238 288 253 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2090 2399 2938 3904 4769 5067 2090 Size: 309 539 966 865 298 2802 Fragments arranged by size: 2802 966 865 539 309 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2237 3014 4442 882 Size: 617 738 777 1428 2219 Fragments arranged by size: 2219 1428 777 738 617 BsaAI yAC'GTr Cuts at: 493 1204 3570 493 Size: 711 2366 2702 Fragments arranged by size: 2702 2366 711 BsaBI GATnn'nnATC Cuts at: 2189 2264 5773 2189 Size: 75 3509 2195 Fragments arranged by size: 3509 2195 75 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2283 2772 3586 3968 Size: 53 83 186 936 750 489 814 382 Cuts at: 3968 4603 275 Size: 635 1451 Fragments arranged by size: 1451 936 814 750 635 489 382 186 83 53 BsaWI w'CCGG_w Cuts at: 3050 4212 4610 4884 5031 5442 3050 Size: 1162 398 274 147 411 3387 Fragments arranged by size: 3387 1162 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2198 2228 2864 2894 3045 Size: 30 165 30 795 30 636 30 151 Cuts at: 3045 3075 1178 Size: 30 3882 Fragments arranged by size: 3882 795 636 165 151 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2288 2356 2423 1186 Size: 1102 68 67 4542 Fragments arranged by size: 4542 1102 68 67 BseYI C'CCAG_C Cuts at: 2961 4982 5478 2961 Size: 2021 496 3262 Fragments arranged by size: 3262 2021 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5206 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2137 3229 3990 5018 1540 Size: 275 186 136 1092 761 1028 2301 Fragments arranged by size: 2301 1092 1028 761 275 186 136 BsiHKAI G_wGCw'C Cuts at: 2697 3840 3925 4996 2697 Size: 1143 85 1071 3480 Fragments arranged by size: 3480 1143 1071 85 BsmI GAATG_Cn' Cuts at: 3290 3383 3290 Size: 93 5686 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2326 2697 2985 3098 3840 3925 4996 Size: 399 536 371 288 113 742 85 1071 Cuts at: 4996 1391 Size: 2174 Fragments arranged by size: 2174 1071 742 536 399 371 288 113 85 BspEI T'CCGG_A Cuts at: 3050 5442 3050 Size: 2392 3387 BspHI T'CATG_A Cuts at: 3668 5398 3668 Size: 1730 4049 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2738 3134 844 Size: 1894 396 3489 Fragments arranged by size: 3489 1894 396 BsrBI CCG'CTC Cuts at: 1394 1823 3666 1394 Size: 429 1843 3507 Fragments arranged by size: 3507 1843 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2506 4274 4448 65 Size: 1183 1258 1768 174 1396 Fragments arranged by size: 1768 1396 1258 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2931 2967 4423 4610 1700 Size: 110 1121 36 1456 187 2869 Fragments arranged by size: 2869 1456 1121 187 110 36 BsrGI T'GTAC_A Cuts at: 96 2622 96 Size: 2526 3253 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5779 BssSI C'ACGA_G Cuts at: 2812 3839 4851 2812 Size: 1027 1012 3740 Fragments arranged by size: 3740 1027 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 2546 2971 794 Size: 1752 425 3602 Fragments arranged by size: 3602 1752 425 BstBI TT'CG_AA Cuts at: 4583 4583 Size: 5779 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5779 BstXI CCAn_nnnn'nTGG Cuts at: 2948 2948 Size: 5779 BstYI r'GATC_y Cuts at: 1972 2235 2259 2799 3109 3476 3861 3878 5330 Size: 263 24 540 310 367 385 17 1452 Cuts at: 5330 5416 5428 5774 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 540 385 367 346 310 263 86 24 17 12 Bsu36I CC'TnA_GG Cuts at: 2024 2024 Size: 5779 BtgI C'CryG_G Cuts at: 513 1065 2004 2209 513 Size: 552 939 205 4083 Fragments arranged by size: 4083 939 552 205 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5040 Fragments arranged by size: 5040 683 56 BtsI GCAGTG_nn' Cuts at: 3295 4089 4109 3295 Size: 794 20 4965 Fragments arranged by size: 4965 794 20 ClaI AT'CG_AT Cuts at: 1554 2793 1554 Size: 1239 4540 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5744 DraI TTT'AAA Cuts at: 3083 3083 Size: 5779 DraIII CAC_nnn'GTG Cuts at: 2701 2701 Size: 5779 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4786 809 Size: 507 3470 1802 Fragments arranged by size: 3470 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3015 3226 4115 Size: 54 1425 50 275 18 1185 211 889 Cuts at: 4115 5744 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1185 889 275 211 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3226 1537 Size: 275 1414 4090 Fragments arranged by size: 4090 1414 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3709 1353 Size: 2356 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2359 4366 4740 4886 387 Size: 1064 138 770 2007 374 146 1280 Fragments arranged by size: 2007 1280 1064 770 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2623 3010 3842 4439 5226 5494 1239 Size: 393 991 387 832 597 787 268 1524 Fragments arranged by size: 1524 991 832 787 597 393 387 268 EcoNI CCTnn'n_nnAGG Cuts at: 2330 2438 2860 2330 Size: 108 422 5249 Fragments arranged by size: 5249 422 108 EcoO109I rG'GnC_Cy Cuts at: 2410 2743 2410 Size: 333 5446 EcoRI G'AATT_C Cuts at: 3088 3466 3088 Size: 378 5401 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2932 2932 Size: 5779 FspI TGC'GCA Cuts at: 4285 4285 Size: 5779 HaeII r_GCGC'y Cuts at: 1678 2104 2485 4926 1678 Size: 426 381 2441 2531 Fragments arranged by size: 2531 2441 426 381 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4420 4452 4494 4526 1373 Size: 32 135 32 2848 32 42 32 2626 Fragments arranged by size: 2848 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2206 3123 3369 669 Size: 988 281 268 917 246 3079 Fragments arranged by size: 3079 988 917 281 268 246 HindIII A'AGCT_T Cuts at: 748 2945 748 Size: 2197 3582 HpaI GTT'AAC Cuts at: 3369 3369 Size: 5779 KpnI G_GTAC'C Cuts at: 3104 3104 Size: 5779 MfeI C'AATT_G Cuts at: 3378 3378 Size: 5779 MluI A'CGCG_T Cuts at: 5655 5655 Size: 5779 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2380 2685 4893 5077 1305 Size: 1075 305 2208 184 2007 Fragments arranged by size: 2208 2007 1075 305 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 3957 Fragments arranged by size: 3957 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2270 2793 3737 4096 4255 518 Size: 648 1104 523 944 359 159 2042 Fragments arranged by size: 2042 1104 944 648 523 359 159 MspA1I CmG'CkG Cuts at: 1606 2782 3195 3872 5020 5265 1606 Size: 1176 413 677 1148 245 2120 Fragments arranged by size: 2120 1176 1148 677 413 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4288 Fragments arranged by size: 4288 939 552 NdeI CA'TA_TG Cuts at: 387 2545 387 Size: 2158 3621 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5779 NotI GC'GGCC_GC Cuts at: 3226 3226 Size: 5779 NspI r_CATG'y Cuts at: 1132 1229 2761 3137 4682 1132 Size: 97 1532 376 1545 2229 Fragments arranged by size: 2229 1545 1532 376 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5779 PciI A'CATG_T Cuts at: 4678 4678 Size: 5779 PflMI CCAn_nnn'nTGG Cuts at: 1108 2392 1108 Size: 1284 4495 PmeI GTTT'AAAC Cuts at: 3083 3083 Size: 5779 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3848 3880 5361 5393 3848 Size: 32 1481 32 4234 Fragments arranged by size: 4234 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2410 2743 2410 Size: 333 5446 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5779 PsiI TTA'TAA Cuts at: 3349 3349 Size: 5779 PspXI vC'TCGA_Gb Cuts at: 2300 2300 Size: 5779 PstI C_TGCA'G Cuts at: 830 2568 2766 2823 3131 830 Size: 1738 198 57 308 3478 Fragments arranged by size: 3478 1738 308 198 57 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5779 SalI G'TCGA_C Cuts at: 1936 3121 1936 Size: 1185 4594 SbfI CC_TGCA'GG Cuts at: 3131 3131 Size: 5779 ScaI AGT'ACT Cuts at: 2381 4027 5739 2381 Size: 1646 1712 2421 Fragments arranged by size: 2421 1712 1646 SfcI C'TryA_G Cuts at: 826 1045 2564 2762 2819 3127 4262 4943 5134 Size: 219 1519 198 57 308 1135 681 191 Cuts at: 5134 826 Size: 1471 Fragments arranged by size: 1519 1471 1135 681 308 219 198 191 57 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5779 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5779 SmaI CCC'GGG Cuts at: 1872 3106 1872 Size: 1234 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2105 2300 2414 2917 3883 4784 Size: 197 926 162 195 114 503 966 901 Cuts at: 4784 5046 820 Size: 262 1553 Fragments arranged by size: 1553 966 926 901 503 262 197 195 162 114 SnaBI TAC'GTA Cuts at: 493 3570 493 Size: 3077 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5779 SphI G_CATG'C Cuts at: 1132 2761 3137 1132 Size: 1629 376 3774 Fragments arranged by size: 3774 1629 376 SspI AAT'ATT Cuts at: 5 52 3703 5 Size: 47 3651 2081 Fragments arranged by size: 3651 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5779 StyI C'CwwG_G Cuts at: 513 1065 2004 2586 3218 513 Size: 552 939 582 632 3074 Fragments arranged by size: 3074 939 632 582 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3806 3823 3976 4161 4606 325 Size: 1255 2226 17 153 185 445 1498 Fragments arranged by size: 2226 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2379 2622 4025 Size: 274 80 33 51 1443 402 243 1403 Cuts at: 4025 5737 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1403 402 274 243 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2402 2434 3045 3077 5739 5771 2402 Size: 32 611 32 2662 32 2410 Fragments arranged by size: 2662 2410 611 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2399 1316 Size: 1083 4696 XbaI T'CTAG_A Cuts at: 3115 3115 Size: 5779 XhoI C'TCGA_G Cuts at: 1943 2300 1943 Size: 357 5422 XmaI C'CCGG_G Cuts at: 1870 3104 1870 Size: 1234 4545 XmnI GAAnn'nnTTC Cuts at: 3908 3908 Size: 5779 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 2773 3587 276 Size: 53 83 186 936 1239 814 2468 Fragments arranged by size: 2468 1239 936 814 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AfeI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspXI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AloI ApaI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PspOMI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI