Restriction map for
pFN21AB8726
(Linear) (Six-base) MAPSORT of: KIBB8726.seq Check: 6692 from: 1 to: 1074
KIBB8726.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AatII G_ACGT'C
Cuts at: 0 770 1074
Size: 770 304
AfeI AGC'GCT
Cuts at: 0 478 1074
Size: 478 596
AvaI C'yCGr_G
Cuts at: 0 173 295 550 979 1074
Size: 173 122 255 429 95
Fragments arranged by size:
429 255 173 122 95
BanI G'GyrC_C
Cuts at: 0 975 1074
Size: 975 99
BanII G_rGCy'C
Cuts at: 0 321 1074
Size: 321 753
BbsI GAAGACnn'nnnn_
Cuts at: 0 833 1074
Size: 833 241
BbvCI CC'TCA_GC
Cuts at: 0 511 799 1052 1074
Size: 511 288 253 22
Fragments arranged by size:
511 288 253 22
BciVI GTATCCnnnnn_n'
Cuts at: 0 688 1074
Size: 688 386
BglII A'GATC_T
Cuts at: 0 230 1074
Size: 230 844
Bme1580I G_kGCm'C
Cuts at: 0 980 1074
Size: 980 94
BmgBI CAC'GTC
Cuts at: 0 333 839 1074
Size: 333 506 235
Fragments arranged by size:
506 333 235
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1005 1074
Size: 618 387 69
Fragments arranged by size:
618 387 69
Bpu10I CC'TnA_GC
Cuts at: 0 511 799 1052 1074
Size: 511 288 253 22
Fragments arranged by size:
511 288 253 22
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 85 394 933 1074
Size: 85 309 539 141
Fragments arranged by size:
539 309 141 85
BsaI GGTCTCn'nnnn_
Cuts at: 0 232 1009 1074
Size: 232 777 65
Fragments arranged by size:
777 232 65
BsaBI GATnn'nnATC
Cuts at: 0 184 259 1074
Size: 184 75 815
Fragments arranged by size:
815 184 75
BsaHI Gr'CG_yC
Cuts at: 0 278 767 1074
Size: 278 489 307
Fragments arranged by size:
489 307 278
BsaWI w'CCGG_w
Cuts at: 0 1045 1074
Size: 1045 29
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 0 193 223 859 889 1040 1070 1074
Size: 193 30 636 30 151 30 4
Fragments arranged by size:
636 193 151 30 30 30 4
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 283 351 418 1074
Size: 283 68 67 656
Fragments arranged by size:
656 283 68 67
BseYI C'CCAG_C
Cuts at: 0 956 1074
Size: 956 118
BsiEI CG_ry'CG
Cuts at: 0 132 1074
Size: 132 942
BsiHKAI G_wGCw'C
Cuts at: 0 692 1074
Size: 692 382
Bsp1286I G_dGCh'C
Cuts at: 0 321 692 980 1074
Size: 321 371 288 94
Fragments arranged by size:
371 321 288 94
BspEI T'CCGG_A
Cuts at: 0 1045 1074
Size: 1045 29
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 733 1074
Size: 733 341
BsrDI GCAATG_nn'
Cuts at: 0 501 1074
Size: 501 573
BsrFI r'CCGG_y
Cuts at: 0 926 962 1074
Size: 926 36 112
Fragments arranged by size:
926 112 36
BsrGI T'GTAC_A
Cuts at: 0 617 1074
Size: 617 457
BssSI C'ACGA_G
Cuts at: 0 807 1074
Size: 807 267
BstAPI GCAn_nnn'nTGC
Cuts at: 0 541 966 1074
Size: 541 425 108
Fragments arranged by size:
541 425 108
BstXI CCAn_nnnn'nTGG
Cuts at: 0 943 1074
Size: 943 131
BstYI r'GATC_y
Cuts at: 0 230 254 794 1074
Size: 230 24 540 280
Fragments arranged by size:
540 280 230 24
Bsu36I CC'TnA_GG
Cuts at: 0 19 1074
Size: 19 1055
BtgI C'CryG_G
Cuts at: 0 204 1074
Size: 204 870
ClaI AT'CG_AT
Cuts at: 0 788 1074
Size: 788 286
DraIII CAC_nnn'GTG
Cuts at: 0 696 1074
Size: 696 378
EaeI y'GGCC_r
Cuts at: 0 1010 1074
Size: 1010 64
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 354 1074
Size: 354 720
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 618 1005 1074
Size: 618 387 69
Fragments arranged by size:
618 387 69
EcoNI CCTnn'n_nnAGG
Cuts at: 0 325 433 855 1074
Size: 325 108 422 219
Fragments arranged by size:
422 325 219 108
EcoO109I rG'GnC_Cy
Cuts at: 0 405 738 1074
Size: 405 333 336
Fragments arranged by size:
405 336 333
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 927 1074
Size: 927 147
HaeII r_GCGC'y
Cuts at: 0 99 480 1074
Size: 99 381 594
Fragments arranged by size:
594 381 99
HincII GTy'rAC
Cuts at: 0 201 1074
Size: 201 873
HindIII A'AGCT_T
Cuts at: 0 940 1074
Size: 940 134
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 375 680 1074
Size: 375 305 394
Fragments arranged by size:
394 375 305
MslI CAynn'nnrTG
Cuts at: 0 265 788 1074
Size: 265 523 286
Fragments arranged by size:
523 286 265
MspA1I CmG'CkG
Cuts at: 0 777 1074
Size: 777 297
NdeI CA'TA_TG
Cuts at: 0 540 1074
Size: 540 534
NspI r_CATG'y
Cuts at: 0 756 1074
Size: 756 318
PflMI CCAn_nnn'nTGG
Cuts at: 0 387 1074
Size: 387 687
PpuMI rG'GwC_Cy
Cuts at: 0 405 738 1074
Size: 405 333 336
Fragments arranged by size:
405 336 333
PspXI vC'TCGA_Gb
Cuts at: 0 295 1074
Size: 295 779
PstI C_TGCA'G
Cuts at: 0 563 761 818 1074
Size: 563 198 57 256
Fragments arranged by size:
563 256 198 57
ScaI AGT'ACT
Cuts at: 0 376 1074
Size: 376 698
SfcI C'TryA_G
Cuts at: 0 559 757 814 1074
Size: 559 198 57 260
Fragments arranged by size:
559 260 198 57
SmlI C'TyrA_G
Cuts at: 0 100 295 409 912 1074
Size: 100 195 114 503 162
Fragments arranged by size:
503 195 162 114 100
SphI G_CATG'C
Cuts at: 0 756 1074
Size: 756 318
StyI C'CwwG_G
Cuts at: 0 581 1074
Size: 581 493
TatI w'GTAC_w
Cuts at: 0 374 617 1074
Size: 374 243 457
Fragments arranged by size:
457 374 243
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 397 429 1040 1072 1074
Size: 397 32 611 32 2
Fragments arranged by size:
611 397 32 32 2
Tth111I GACn'n_nGTC
Cuts at: 0 394 1074
Size: 394 680
XhoI C'TCGA_G
Cuts at: 0 295 1074
Size: 295 779
ZraI GAC'GTC
Cuts at: 0 768 1074
Size: 768 306
Enzymes that do cut:
AatII AfeI AvaI BanI BanII BbsI BbvCI BciVI
BglII Bme1580I BmgBI BpmI Bpu10I BpuEI BsaI BsaBI
BsaHI BsaWI BsaXI BseRI BseYI BsiEI BsiHKAI Bsp1286I
BspEI BspMI BsrDI BsrFI BsrGI BssSI BstAPI BstXI
BstYI Bsu36I BtgI ClaI DraIII EaeI EciI Eco57MI
EcoNI EcoO109I FalI HaeII HincII HindIII MmeI MslI
MspA1I NdeI NspI PflMI PpuMI PspXI PstI ScaI
SfcI SmlI SphI StyI TatI TstI Tth111I XhoI
ZraI
Enzymes that do not cut:
AarI AccI Acc65I AclI AcuI AflII AflIII AgeI
AhdI AleI AlfI AloI AlwNI ApaI ApaLI ApoI
AscI AseI AsiSI AvrII BaeI BamHI BbeI BcgI
BclI BfrBI BglI BlpI BmrI BmtI BplI BsaAI
BsgI BsiWI BsmI BsmBI BspHI BsrBI BssHII BstBI
BstEII BstZ17I BtgZI BtsI CspCI DraI DrdI EagI
EarI EcoICRI EcoRI EcoRV FseI FspI FspAI Hin4I
HpaI KasI KpnI MfeI MluI MscI NaeI NarI
NcoI NgoMIV NheI NotI NruI NsiI PacI PasI
PciI PfoI PmeI PmlI PpiI PshAI PsiI PspOMI
PsrI PvuI PvuII RsrII SacI SacII SalI SanDI
SapI SbfI SexAI SfiI SfoI SgfI SgrAI SmaI
SnaBI SpeI SrfI SspI StuI SwaI TaqII XbaI
XcmI XmaI XmnI