(Circular) (Six-base) MAPSORT of: pFN21AB8723.seq Check: 6696 from: 1 to: 6232 pFN21AB8723 6232 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2249 2249 Size: 6232 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4042 278 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2541 2794 3575 1937 Size: 604 253 781 4594 Fragments arranged by size: 4594 781 604 253 Acc65I G'GTAC_C Cuts at: 3362 3553 3362 Size: 191 6041 AclI AA'CG_TT Cuts at: 1563 3531 4359 4732 1563 Size: 1968 828 373 3063 Fragments arranged by size: 3063 1968 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2129 2231 2663 4295 5679 5947 1632 Size: 497 102 432 1632 1384 268 1917 Fragments arranged by size: 1917 1632 1384 497 432 268 102 AflII C'TTAA_G Cuts at: 820 1017 3235 820 Size: 197 2218 3817 Fragments arranged by size: 3817 2218 197 AflIII A'CryG_T Cuts at: 1203 5131 6108 1203 Size: 3928 977 1327 Fragments arranged by size: 3928 1327 977 AgeI A'CCGG_T Cuts at: 5063 5063 Size: 6232 AhdI GACnn_n'nnGTC Cuts at: 4961 4961 Size: 6232 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6232 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6232 AlwNI CAG_nnn'CTG Cuts at: 2104 2209 2896 3151 3975 5547 2104 Size: 105 687 255 824 1572 2789 Fragments arranged by size: 2789 1572 824 687 255 105 ApaLI G'TGCA_C Cuts at: 4289 5445 4289 Size: 1156 5076 ApoI r'AATT_y Cuts at: 1493 2756 3250 3541 3767 3919 6028 1493 Size: 1263 494 291 226 152 2109 1697 Fragments arranged by size: 2109 1697 1263 494 291 226 152 AseI AT'TA_AT Cuts at: 160 1032 2784 4786 160 Size: 872 1752 2002 1606 Fragments arranged by size: 2002 1752 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 AvaI C'yCGr_G Cuts at: 1870 1943 3101 3557 1870 Size: 73 1158 456 4545 Fragments arranged by size: 4545 1158 456 73 BamHI G'GATC_C Cuts at: 3562 3929 3562 Size: 367 5865 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2561 3362 3553 5008 618 Size: 325 217 627 774 801 191 1455 1842 Fragments arranged by size: 1842 1455 801 774 627 325 217 191 BanII G_rGCy'C Cuts at: 1391 2160 3108 3504 3551 1391 Size: 769 948 396 47 4072 Fragments arranged by size: 4072 948 769 396 47 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 5261 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4423 4457 1903 Size: 34 2486 34 3678 Fragments arranged by size: 3678 2486 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3336 4124 5334 3336 Size: 788 1210 4234 Fragments arranged by size: 4234 1210 788 BclI T'GATC_A Cuts at: 1548 3181 1548 Size: 1633 4599 BfrBI ATG'CAT Cuts at: 2819 3025 2819 Size: 206 6026 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4843 136 Size: 107 122 71 631 605 3171 1525 Fragments arranged by size: 3171 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6227 6227 Size: 6232 BlpI GC'TnA_GC Cuts at: 2460 3302 3649 2460 Size: 842 347 5043 Fragments arranged by size: 5043 842 347 Bme1580I G_kGCm'C Cuts at: 1790 3515 4293 5449 1790 Size: 1725 778 1156 2573 Fragments arranged by size: 2573 1725 1156 778 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6232 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2857 3963 4921 Size: 416 53 83 399 24 1440 1106 958 Cuts at: 4921 442 Size: 1753 Fragments arranged by size: 1753 1440 1106 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6232 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3148 3148 Size: 6232 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2159 2306 3128 3143 3359 3395 4892 1239 Size: 920 147 822 15 216 36 1497 2579 Fragments arranged by size: 2579 1497 920 822 216 147 36 15 Bpu10I CC'TnA_GC Cuts at: 2954 3209 3404 2954 Size: 255 195 5782 Fragments arranged by size: 5782 255 195 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2537 4357 5222 5520 2537 Size: 1820 865 298 3249 Fragments arranged by size: 3249 1820 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2282 4895 882 Size: 617 783 2613 2219 Fragments arranged by size: 2613 2219 783 617 BsaAI yAC'GTr Cuts at: 493 1204 2962 3319 4023 493 Size: 711 1758 357 704 2702 Fragments arranged by size: 2702 1758 711 704 357 BsaBI GATnn'nnATC Cuts at: 6226 6226 Size: 6232 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4039 4421 5056 275 Size: 53 83 186 936 2506 382 635 1451 Fragments arranged by size: 2506 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4665 5063 5337 5484 5895 4665 Size: 398 274 147 411 5002 Fragments arranged by size: 5002 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3401 3431 1178 Size: 30 165 30 1998 30 3979 Fragments arranged by size: 3979 1998 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2789 2792 1186 Size: 1603 3 4626 Fragments arranged by size: 4626 1603 3 BseYI C'CCAG_C Cuts at: 3041 3222 3434 5435 5931 3041 Size: 181 212 2001 496 3342 Fragments arranged by size: 3342 2001 496 212 181 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2243 2714 1163 Size: 573 507 471 4681 Fragments arranged by size: 4681 573 507 471 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3682 4443 5471 1540 Size: 275 186 1681 761 1028 2301 Fragments arranged by size: 2301 1681 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2160 2325 2501 2538 4293 4378 5449 2160 Size: 165 176 37 1755 85 1071 2943 Fragments arranged by size: 2943 1755 1071 176 165 85 37 BsmI GAATG_Cn' Cuts at: 3743 3836 3743 Size: 93 6139 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2160 2325 2501 2538 3108 3504 3515 Size: 399 370 165 176 37 570 396 11 Cuts at: 3515 3551 4293 4378 5449 1391 Size: 36 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 570 399 396 370 176 165 85 37 36 11 BspEI T'CCGG_A Cuts at: 5895 5895 Size: 6232 BspHI T'CATG_A Cuts at: 4121 5851 4121 Size: 1730 4502 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2249 3587 844 Size: 1405 1338 3489 Fragments arranged by size: 3489 1405 1338 BsrBI CCG'CTC Cuts at: 1394 1823 4119 1394 Size: 429 2296 3507 Fragments arranged by size: 3507 2296 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2127 2223 2661 4727 4901 65 Size: 1183 879 96 438 2066 174 1396 Fragments arranged by size: 2066 1396 1183 879 438 174 96 BsrFI r'CCGG_y Cuts at: 1700 1810 3000 4876 5063 1700 Size: 110 1190 1876 187 2869 Fragments arranged by size: 2869 1876 1190 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6232 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6232 BssSI C'ACGA_G Cuts at: 4292 5304 4292 Size: 1012 5220 BstAPI GCAn_nnn'nTGC Cuts at: 794 2104 2896 794 Size: 1310 792 4130 Fragments arranged by size: 4130 1310 792 BstBI TT'CG_AA Cuts at: 5036 5036 Size: 6232 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6232 BstYI r'GATC_y Cuts at: 1972 3562 3929 4314 4331 5783 5869 5881 6227 Size: 1590 367 385 17 1452 86 12 346 Cuts at: 6227 1972 Size: 1977 Fragments arranged by size: 1977 1590 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 2072 3358 513 Size: 552 939 68 1286 3387 Fragments arranged by size: 3387 1286 939 552 68 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5493 Fragments arranged by size: 5493 683 56 BtsI GCAGTG_nn' Cuts at: 2897 3214 3748 4542 4562 2897 Size: 317 534 794 20 4567 Fragments arranged by size: 4567 794 534 317 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6232 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6197 DraI TTT'AAA Cuts at: 3536 3536 Size: 6232 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2410 5239 809 Size: 507 1094 2829 1802 Fragments arranged by size: 2829 1802 1094 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2124 3679 4568 Size: 54 1425 50 275 18 294 1555 889 Cuts at: 4568 6197 8 Size: 1629 43 Fragments arranged by size: 1629 1555 1425 889 294 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3679 1537 Size: 275 1867 4090 Fragments arranged by size: 4090 1867 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2165 2177 2841 4162 1353 Size: 812 12 664 1321 3423 Fragments arranged by size: 3423 1321 812 664 12 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4819 5193 5339 387 Size: 1064 138 3230 374 146 1280 Fragments arranged by size: 3230 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2158 2158 Size: 6232 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2159 2231 2306 2663 3128 3143 Size: 393 497 30 72 75 357 465 15 Cuts at: 3143 3359 3395 4295 4892 5679 5947 1239 Size: 216 36 900 597 787 268 1524 Fragments arranged by size: 1524 900 787 597 497 465 393 357 268 216 75 72 36 30 15 EcoNI CCTnn'n_nnAGG Cuts at: 2108 2330 2108 Size: 222 6010 EcoO109I rG'GnC_Cy Cuts at: 2119 3148 2119 Size: 1029 5203 EcoRI G'AATT_C Cuts at: 3250 3541 3919 3250 Size: 291 378 5563 Fragments arranged by size: 5563 378 291 EcoRV GAT'ATC Cuts at: 2671 2671 Size: 6232 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2321 2321 Size: 6232 FspI TGC'GCA Cuts at: 4738 4738 Size: 6232 HaeII r_GCGC'y Cuts at: 1678 5379 1678 Size: 3701 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2770 2787 2802 2819 4873 Size: 32 135 32 1198 17 15 17 2054 Cuts at: 4873 4905 4947 4979 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 2054 1198 135 42 32 32 32 32 17 17 15 HincII GTy'rAC Cuts at: 669 1657 1938 3576 3822 669 Size: 988 281 1638 246 3079 Fragments arranged by size: 3079 1638 988 281 246 HindIII A'AGCT_T Cuts at: 748 3238 748 Size: 2490 3742 HpaI GTT'AAC Cuts at: 3822 3822 Size: 6232 KpnI G_GTAC'C Cuts at: 3366 3557 3366 Size: 191 6041 MfeI C'AATT_G Cuts at: 3831 3831 Size: 6232 MluI A'CGCG_T Cuts at: 6108 6108 Size: 6232 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5346 5530 1305 Size: 4041 184 2007 Fragments arranged by size: 4041 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2126 10 Size: 54 1425 343 294 4116 Fragments arranged by size: 4116 1425 343 294 54 MslI CAynn'nnrTG Cuts at: 518 1166 2051 2057 2814 4190 4549 4708 518 Size: 648 885 6 757 1376 359 159 2042 Fragments arranged by size: 2042 1376 885 757 648 359 159 6 MspA1I CmG'CkG Cuts at: 1606 2698 3648 4325 5473 5718 1606 Size: 1092 950 677 1148 245 2120 Fragments arranged by size: 2120 1148 1092 950 677 245 NaeI GCC'GGC Cuts at: 3002 3002 Size: 6232 NcoI C'CATG_G Cuts at: 513 1065 2004 3358 513 Size: 552 939 1354 3387 Fragments arranged by size: 3387 1354 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6232 NgoMIV G'CCGG_C Cuts at: 3000 3000 Size: 6232 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6232 NotI GC'GGCC_GC Cuts at: 3679 3679 Size: 6232 NsiI A_TGCA'T Cuts at: 2821 3027 2821 Size: 206 6026 NspI r_CATG'y Cuts at: 1132 1229 3398 3590 5135 1132 Size: 97 2169 192 1545 2229 Fragments arranged by size: 2229 2169 1545 192 97 PasI CC'CwG_GG Cuts at: 1348 2151 1348 Size: 803 5429 PciI A'CATG_T Cuts at: 5131 5131 Size: 6232 PflMI CCAn_nnn'nTGG Cuts at: 1108 2352 3151 1108 Size: 1244 799 4189 Fragments arranged by size: 4189 1244 799 PfoI T'CCnGG_A Cuts at: 3144 3144 Size: 6232 PmeI GTTT'AAAC Cuts at: 3536 3536 Size: 6232 PmlI CAC'GTG Cuts at: 2962 3319 2962 Size: 357 5875 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4301 4333 5814 5846 4301 Size: 32 1481 32 4687 Fragments arranged by size: 4687 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3148 3148 Size: 6232 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6232 PsiI TTA'TAA Cuts at: 3802 3802 Size: 6232 PstI C_TGCA'G Cuts at: 830 2892 2901 3288 3584 830 Size: 2062 9 387 296 3478 Fragments arranged by size: 3478 2062 387 296 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6232 PvuII CAG'CTG Cuts at: 2698 2698 Size: 6232 SacI G_AGCT'C Cuts at: 2160 2160 Size: 6232 SalI G'TCGA_C Cuts at: 1936 3574 1936 Size: 1638 4594 SapI GCTCTTCn'nnn_ Cuts at: 2165 2841 2165 Size: 676 5556 SbfI CC_TGCA'GG Cuts at: 2901 3584 2901 Size: 683 5549 ScaI AGT'ACT Cuts at: 4480 6192 4480 Size: 1712 4520 SexAI A'CCwGG_T Cuts at: 3150 3150 Size: 6232 SfcI C'TryA_G Cuts at: 826 1045 2888 2897 3284 3580 4715 5396 5587 Size: 219 1843 9 387 296 1135 681 191 Cuts at: 5587 826 Size: 1471 Fragments arranged by size: 1843 1471 1135 681 387 296 219 191 9 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6232 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6232 SmaI CCC'GGG Cuts at: 1872 3559 1872 Size: 1687 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2552 3235 4336 5237 5499 820 Size: 197 926 609 683 1101 901 262 1553 Fragments arranged by size: 1553 1101 926 901 683 609 262 197 SnaBI TAC'GTA Cuts at: 493 4023 493 Size: 3530 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6232 SphI G_CATG'C Cuts at: 1132 3398 3590 1132 Size: 2266 192 3774 Fragments arranged by size: 3774 2266 192 SspI AAT'ATT Cuts at: 5 52 4156 5 Size: 47 4104 2081 Fragments arranged by size: 4104 2081 47 StuI AGG'CCT Cuts at: 1517 2228 1517 Size: 711 5521 StyI C'CwwG_G Cuts at: 513 1065 2004 2748 2973 3358 3671 513 Size: 552 939 744 225 385 313 3074 Fragments arranged by size: 3074 939 744 552 385 313 225 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3104 4259 4276 4429 4614 5059 325 Size: 1255 1524 1155 17 153 185 445 1498 Fragments arranged by size: 1524 1498 1255 1155 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2433 2617 4478 Size: 274 80 33 51 1443 456 184 1861 Cuts at: 4478 6190 96 Size: 1712 138 Fragments arranged by size: 1861 1712 1443 456 274 184 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2304 2336 3401 3433 6192 6224 2304 Size: 32 1065 32 2759 32 2312 Fragments arranged by size: 2759 2312 1065 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 3152 1316 Size: 1836 4396 XbaI T'CTAG_A Cuts at: 3568 3568 Size: 6232 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2134 2134 Size: 6232 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6232 XmaI C'CCGG_G Cuts at: 1870 3557 1870 Size: 1687 4545 XmnI GAAnn'nnTTC Cuts at: 4361 4361 Size: 6232 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4040 276 Size: 53 83 186 936 2506 2468 Fragments arranged by size: 2506 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI EcoRV FalI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PstI PvuI PvuII SacI SalI SapI SbfI ScaI SexAI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI ApaI AscI AvrII BaeI BbeI BbvCI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII FseI FspAI KasI NarI NruI PacI PspOMI PspXI PsrI RsrII SacII SanDI SfiI SfoI SrfI SwaI