(Circular) (Six-base) MAPSORT of: pFN21AB8721.seq Check: 3184 from: 1 to: 6376 pFN21AB8721 6376 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2109 3124 2109 Size: 1015 5361 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4186 278 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 2901 3180 3719 1937 Size: 964 279 539 4594 Fragments arranged by size: 4594 964 539 279 Acc65I G'GTAC_C Cuts at: 3697 3697 Size: 6376 AclI AA'CG_TT Cuts at: 1563 3675 4503 4876 1563 Size: 2112 828 373 3063 Fragments arranged by size: 3063 2112 828 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2007 2393 3359 3471 4439 5823 6091 1632 Size: 375 386 966 112 968 1384 268 1917 Fragments arranged by size: 1917 1384 968 966 386 375 268 112 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6179 AflIII A'CryG_T Cuts at: 1203 5275 6252 1203 Size: 4072 977 1327 Fragments arranged by size: 4072 1327 977 AgeI A'CCGG_T Cuts at: 5207 5207 Size: 6376 AhdI GACnn_n'nnGTC Cuts at: 5105 5105 Size: 6376 AleI CACnn'nnGTG Cuts at: 1166 3420 1166 Size: 2254 4122 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6376 AlwNI CAG_nnn'CTG Cuts at: 2047 2131 2371 3067 3157 3239 3527 4119 5691 Size: 84 240 696 90 82 288 592 1572 Cuts at: 5691 2047 Size: 2732 Fragments arranged by size: 2732 1572 696 592 288 240 90 84 82 ApaI G_GGCC'C Cuts at: 2242 2497 3303 3337 2242 Size: 255 806 34 5281 Fragments arranged by size: 5281 806 255 34 ApaLI G'TGCA_C Cuts at: 2209 3413 4433 5589 2209 Size: 1204 1020 1156 2996 Fragments arranged by size: 2996 1204 1156 1020 ApoI r'AATT_y Cuts at: 1493 3685 3911 4063 6172 1493 Size: 2192 226 152 2109 1697 Fragments arranged by size: 2192 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 2874 4930 160 Size: 872 1842 2056 1606 Fragments arranged by size: 2056 1842 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 AvaI C'yCGr_G Cuts at: 1870 1943 2071 2630 2662 3701 1870 Size: 73 128 559 32 1039 4545 Fragments arranged by size: 4545 1039 559 128 73 32 BamHI G'GATC_C Cuts at: 3706 4073 3706 Size: 367 6009 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2717 3697 5152 618 Size: 325 217 627 930 980 1455 1842 Fragments arranged by size: 1842 1455 980 930 627 325 217 BanII G_rGCy'C Cuts at: 1391 2072 2097 2242 2497 3303 3337 3695 1391 Size: 681 25 145 255 806 34 358 4072 Fragments arranged by size: 4072 806 681 358 255 145 34 25 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3626 928 Size: 971 1727 3678 Fragments arranged by size: 3678 1727 971 BbvCI CC'TCA_GC Cuts at: 3528 3528 Size: 6376 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2305 2339 4567 4601 1903 Size: 34 368 34 2228 34 3678 Fragments arranged by size: 3678 2228 368 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4268 5478 4268 Size: 1210 5166 BclI T'GATC_A Cuts at: 1548 1548 Size: 6376 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2044 2166 2570 Size: 107 122 71 631 605 372 122 404 Cuts at: 2570 2726 3307 4987 136 Size: 156 581 1680 1525 Fragments arranged by size: 1680 1525 631 605 581 404 372 156 122 122 107 71 BglII A'GATC_T Cuts at: 3597 6371 3597 Size: 2774 3602 BlpI GC'TnA_GC Cuts at: 2105 3376 3793 2105 Size: 1271 417 4688 Fragments arranged by size: 4688 1271 417 Bme1580I G_kGCm'C Cuts at: 1790 2213 2242 2497 3303 3337 3417 4437 5593 Size: 423 29 255 806 34 80 1020 1156 Cuts at: 5593 1790 Size: 2573 Fragments arranged by size: 2573 1156 1020 806 423 255 80 34 29 BmgBI CAC'GTC Cuts at: 1318 3391 1318 Size: 2073 4303 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3297 4107 5065 Size: 416 53 83 399 24 1880 810 958 Cuts at: 5065 442 Size: 1753 Fragments arranged by size: 1880 1753 958 810 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 2861 1056 Size: 1805 4571 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2051 2264 2270 2663 5036 1239 Size: 812 213 6 393 2373 2579 Fragments arranged by size: 2579 2373 812 393 213 6 Bpu10I CC'TnA_GC Cuts at: 2604 3528 2604 Size: 924 5452 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4501 5366 5664 4501 Size: 865 298 5213 Fragments arranged by size: 5213 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2903 2964 5039 882 Size: 617 1404 61 2075 2219 Fragments arranged by size: 2219 2075 1404 617 61 BsaAI yAC'GTr Cuts at: 493 1204 4167 493 Size: 711 2963 2702 Fragments arranged by size: 2963 2702 711 BsaBI GATnn'nnATC Cuts at: 2969 6370 2969 Size: 3401 2975 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 4183 4565 5200 275 Size: 53 83 186 936 2650 382 635 1451 Fragments arranged by size: 2650 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4809 5207 5481 5628 6039 4809 Size: 398 274 147 411 5146 Fragments arranged by size: 5146 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2190 2220 1178 Size: 30 165 30 787 30 5334 Fragments arranged by size: 5334 787 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2089 2102 2105 2261 2648 2921 1186 Size: 903 13 3 156 387 273 4641 Fragments arranged by size: 4641 903 387 273 156 13 3 BseYI C'CCAG_C Cuts at: 2040 2257 2568 3067 3239 5579 6075 2040 Size: 217 311 499 172 2340 496 2341 Fragments arranged by size: 2341 2340 499 496 311 217 172 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 3186 1163 Size: 573 1450 4353 Fragments arranged by size: 4353 1450 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2170 3826 4587 5615 1540 Size: 275 186 169 1656 761 1028 2301 Fragments arranged by size: 2301 1656 1028 761 275 186 169 BsiHKAI G_wGCw'C Cuts at: 2213 2256 3417 4437 4522 5593 2213 Size: 43 1161 1020 85 1071 2996 Fragments arranged by size: 2996 1161 1071 1020 85 43 BsmI GAATG_Cn' Cuts at: 3314 3373 3887 3980 3314 Size: 59 514 93 5710 Fragments arranged by size: 5710 514 93 59 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2072 2097 2213 2242 2256 2497 3303 Size: 399 282 25 116 29 14 241 806 Cuts at: 3303 3337 3417 3695 4437 4522 5593 1391 Size: 34 80 278 742 85 1071 2174 Fragments arranged by size: 2174 1071 806 742 399 282 278 241 116 85 80 34 29 25 14 BspEI T'CCGG_A Cuts at: 6039 6039 Size: 6376 BspHI T'CATG_A Cuts at: 4265 5995 4265 Size: 1730 4646 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2109 3124 3731 844 Size: 1265 1015 607 3489 Fragments arranged by size: 3489 1265 1015 607 BsrBI CCG'CTC Cuts at: 1394 1823 4263 1394 Size: 429 2440 3507 Fragments arranged by size: 3507 2440 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4871 5045 65 Size: 1183 3623 174 1396 Fragments arranged by size: 3623 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2169 2378 5020 5207 1700 Size: 110 359 209 2642 187 2869 Fragments arranged by size: 2869 2642 359 209 187 110 BsrGI T'GTAC_A Cuts at: 96 3104 96 Size: 3008 3368 BssHII G'CGCG_C Cuts at: 1925 2622 1925 Size: 697 5679 BssSI C'ACGA_G Cuts at: 3545 4436 5448 3545 Size: 891 1012 4473 Fragments arranged by size: 4473 1012 891 BstAPI GCAn_nnn'nTGC Cuts at: 794 3368 794 Size: 2574 3802 BstBI TT'CG_AA Cuts at: 5180 5180 Size: 6376 BstEII G'GTnAC_C Cuts at: 1184 2185 1184 Size: 1001 5375 BstXI CCAn_nnnn'nTGG Cuts at: 2265 2554 3253 2265 Size: 289 699 5388 Fragments arranged by size: 5388 699 289 BstYI r'GATC_y Cuts at: 1972 2482 3217 3597 3706 4073 4458 4475 5927 Size: 510 735 380 109 367 385 17 1452 Cuts at: 5927 6013 6025 6371 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 735 510 385 380 367 346 109 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2234 2234 Size: 6376 BtgI C'CryG_G Cuts at: 513 1065 2004 2164 2415 2532 3184 513 Size: 552 939 160 251 117 652 3705 Fragments arranged by size: 3705 939 652 552 251 160 117 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5637 Fragments arranged by size: 5637 683 56 BtsI GCAGTG_nn' Cuts at: 2223 2421 2441 2889 3528 3892 4686 4706 2223 Size: 198 20 448 639 364 794 20 3893 Fragments arranged by size: 3893 794 639 448 364 198 20 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6376 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6341 DraI TTT'AAA Cuts at: 3680 3680 Size: 6376 DraIII CAC_nnn'GTG Cuts at: 2217 2217 Size: 6376 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2015 5383 809 Size: 507 699 3368 1802 Fragments arranged by size: 3368 1802 699 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2133 2167 2418 Size: 54 1425 50 275 18 303 34 251 Cuts at: 2418 2436 2454 2700 3570 3823 4712 6341 8 Size: 18 18 246 870 253 889 1629 43 Fragments arranged by size: 1629 1425 889 870 303 275 253 251 246 54 50 43 34 18 18 18 EagI C'GGCC_G Cuts at: 1537 1812 2167 3823 1537 Size: 275 355 1656 4090 Fragments arranged by size: 4090 1656 355 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2079 4306 1353 Size: 726 2227 3423 Fragments arranged by size: 3423 2227 726 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2939 4963 5337 5483 387 Size: 1064 138 1350 2024 374 146 1280 Fragments arranged by size: 2024 1350 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2007 2051 2264 2270 2393 2663 3359 Size: 393 375 44 213 6 123 270 696 Cuts at: 3359 3471 4439 5036 5823 6091 1239 Size: 112 968 597 787 268 1524 Fragments arranged by size: 1524 968 787 696 597 393 375 270 268 213 123 112 44 6 EcoNI CCTnn'n_nnAGG Cuts at: 2984 2984 Size: 6376 EcoO109I rG'GnC_Cy Cuts at: 2238 2239 2431 2471 2494 3299 3334 2238 Size: 1 192 40 23 805 35 5280 Fragments arranged by size: 5280 805 192 40 35 23 1 EcoRI G'AATT_C Cuts at: 3685 4063 3685 Size: 378 5998 FseI GG_CCGG'CC Cuts at: 2382 2382 Size: 6376 FspI TGC'GCA Cuts at: 4882 4882 Size: 6376 HaeII r_GCGC'y Cuts at: 1678 5523 1678 Size: 3845 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 3589 3621 5017 5049 5091 Size: 32 135 32 2017 32 1396 32 42 Cuts at: 5091 5123 1373 Size: 32 2626 Fragments arranged by size: 2626 2017 1396 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2902 3720 3966 669 Size: 988 281 964 818 246 3079 Fragments arranged by size: 3079 988 964 818 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6376 HpaI GTT'AAC Cuts at: 3966 3966 Size: 6376 KpnI G_GTAC'C Cuts at: 3701 3701 Size: 6376 MfeI C'AATT_G Cuts at: 3975 3975 Size: 6376 MluI A'CGCG_T Cuts at: 6252 6252 Size: 6376 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2973 5490 5674 1305 Size: 1668 2517 184 2007 Fragments arranged by size: 2517 2007 1668 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2135 2438 2456 2702 3572 Size: 54 1425 343 303 303 18 246 870 Cuts at: 3572 10 Size: 2814 Fragments arranged by size: 2814 1425 870 343 303 303 246 54 18 MslI CAynn'nnrTG Cuts at: 518 1166 2763 2942 3251 3420 4334 4693 4852 Size: 648 1597 179 309 169 914 359 159 Cuts at: 4852 518 Size: 2042 Fragments arranged by size: 2042 1597 914 648 359 309 179 169 159 MspA1I CmG'CkG Cuts at: 1606 2044 2368 2864 3792 4469 5617 5862 1606 Size: 438 324 496 928 677 1148 245 2120 Fragments arranged by size: 2120 1148 928 677 496 438 324 245 NaeI GCC'GGC Cuts at: 2171 2380 2171 Size: 209 6167 NcoI C'CATG_G Cuts at: 513 1065 2004 2532 513 Size: 552 939 528 4357 Fragments arranged by size: 4357 939 552 528 NdeI CA'TA_TG Cuts at: 387 387 Size: 6376 NgoMIV G'CCGG_C Cuts at: 2169 2378 2169 Size: 209 6167 NheI G'CTAG_C Cuts at: 1052 2857 1052 Size: 1805 4571 NotI GC'GGCC_GC Cuts at: 3823 3823 Size: 6376 NspI r_CATG'y Cuts at: 1132 1229 3734 5279 1132 Size: 97 2505 1545 2229 Fragments arranged by size: 2505 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 3151 1348 Size: 1803 4573 PciI A'CATG_T Cuts at: 5275 5275 Size: 6376 PflMI CCAn_nnn'nTGG Cuts at: 1108 2047 2131 2467 3239 1108 Size: 939 84 336 772 4245 Fragments arranged by size: 4245 939 772 336 84 PfoI T'CCnGG_A Cuts at: 2274 2400 3232 2274 Size: 126 832 5418 Fragments arranged by size: 5418 832 126 PmeI GTTT'AAAC Cuts at: 3680 3680 Size: 6376 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4445 4477 5958 5990 4445 Size: 32 1481 32 4831 Fragments arranged by size: 4831 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2431 2431 Size: 6376 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6376 PsiI TTA'TAA Cuts at: 3946 3946 Size: 6376 PspOMI G'GGCC_C Cuts at: 2238 2493 3299 3333 2238 Size: 255 806 34 5281 Fragments arranged by size: 5281 806 255 34 PstI C_TGCA'G Cuts at: 830 2122 2428 2869 3728 830 Size: 1292 306 441 859 3478 Fragments arranged by size: 3478 1292 859 441 306 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6376 PvuII CAG'CTG Cuts at: 2368 2864 2368 Size: 496 5880 SalI G'TCGA_C Cuts at: 1936 2900 3718 1936 Size: 964 818 4594 Fragments arranged by size: 4594 964 818 SbfI CC_TGCA'GG Cuts at: 3728 3728 Size: 6376 ScaI AGT'ACT Cuts at: 4624 6336 4624 Size: 1712 4664 SfcI C'TryA_G Cuts at: 826 1045 2118 2424 2865 3539 3552 3724 4859 Size: 219 1073 306 441 674 13 172 1135 Cuts at: 4859 5540 5731 826 Size: 681 191 1471 Fragments arranged by size: 1471 1135 1073 681 674 441 306 219 191 172 13 SfiI GGCCn_nnn'nGGCC Cuts at: 2166 3307 2166 Size: 1141 5235 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6376 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6376 SmaI CCC'GGG Cuts at: 1872 2632 2664 3703 1872 Size: 760 32 1039 4545 Fragments arranged by size: 4545 1039 760 32 SmlI C'TyrA_G Cuts at: 820 1017 1943 4480 5381 5643 820 Size: 197 926 2537 901 262 1553 Fragments arranged by size: 2537 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4167 493 Size: 3674 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6376 SphI G_CATG'C Cuts at: 1132 3734 1132 Size: 2602 3774 SspI AAT'ATT Cuts at: 5 52 4300 5 Size: 47 4248 2081 Fragments arranged by size: 4248 2081 47 StuI AGG'CCT Cuts at: 1517 2408 1517 Size: 891 5485 StyI C'CwwG_G Cuts at: 513 1065 2004 2060 2532 3418 3508 3661 3815 Size: 552 939 56 472 886 90 153 154 Cuts at: 3815 513 Size: 3074 Fragments arranged by size: 3074 939 886 552 472 154 153 90 56 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2271 3164 3428 3648 4403 4420 4573 Size: 1255 691 893 264 220 755 17 153 Cuts at: 4573 4758 5203 325 Size: 185 445 1498 Fragments arranged by size: 1498 1255 893 755 691 445 264 220 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3104 4622 6334 Size: 274 80 33 51 1443 1127 1518 1712 Cuts at: 6334 96 Size: 138 Fragments arranged by size: 1712 1518 1443 1127 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2190 2222 2510 2542 2674 2706 2862 2894 3467 Size: 32 288 32 132 32 156 32 573 Cuts at: 3467 3499 6336 6368 2190 Size: 32 2837 32 2198 Fragments arranged by size: 2837 2198 573 288 156 132 32 32 32 32 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6376 XbaI T'CTAG_A Cuts at: 3712 3712 Size: 6376 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2464 2464 Size: 6376 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6376 XmaI C'CCGG_G Cuts at: 1870 2630 2662 3701 1870 Size: 760 32 1039 4545 Fragments arranged by size: 4545 1039 760 32 XmnI GAAnn'nnTTC Cuts at: 4505 4505 Size: 6376 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4184 276 Size: 53 83 186 936 2650 2468 Fragments arranged by size: 2650 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstXI BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BfrBI BplI BsiWI BsmBI BstZ17I EcoICRI EcoRV FalI FspAI KasI NarI NruI NsiI PacI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI