(Circular) (Six-base) MAPSORT of: pFN21AB8719.seq Check: 5445 from: 1 to: 6133 pFN21AB8719 6133 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2722 2778 2722 Size: 56 6077 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3943 278 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3476 1937 Size: 1539 4594 Acc65I G'GTAC_C Cuts at: 3454 3454 Size: 6133 AclI AA'CG_TT Cuts at: 1563 4260 4633 1563 Size: 2697 373 3063 Fragments arranged by size: 3063 2697 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2755 3095 4196 5580 5848 1632 Size: 1123 340 1101 1384 268 1917 Fragments arranged by size: 1917 1384 1123 1101 340 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5936 AflIII A'CryG_T Cuts at: 1203 5032 6009 1203 Size: 3829 977 1327 Fragments arranged by size: 3829 1327 977 AgeI A'CCGG_T Cuts at: 4964 4964 Size: 6133 AhdI GACnn_n'nnGTC Cuts at: 4862 4862 Size: 6133 AleI CACnn'nnGTG Cuts at: 1166 2357 1166 Size: 1191 4942 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 3420 1184 Size: 2236 3897 AlwNI CAG_nnn'CTG Cuts at: 2354 3876 5448 2354 Size: 1522 1572 3039 Fragments arranged by size: 3039 1572 1522 ApaI G_GGCC'C Cuts at: 2849 3244 2849 Size: 395 5738 ApaLI G'TGCA_C Cuts at: 4190 5346 4190 Size: 1156 4977 ApoI r'AATT_y Cuts at: 1493 2668 3442 3668 3820 5929 1493 Size: 1175 774 226 152 2109 1697 Fragments arranged by size: 2109 1697 1175 774 226 152 AseI AT'TA_AT Cuts at: 160 1032 4687 160 Size: 872 3655 1606 Fragments arranged by size: 3655 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 AvaI C'yCGr_G Cuts at: 1870 1943 2062 2289 2883 3458 1870 Size: 73 119 227 594 575 4545 Fragments arranged by size: 4545 594 575 227 119 73 BamHI G'GATC_C Cuts at: 3463 3830 3463 Size: 367 5766 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2790 3331 3454 4909 618 Size: 325 217 627 1003 541 123 1455 1842 Fragments arranged by size: 1842 1455 1003 627 541 325 217 123 BanII G_rGCy'C Cuts at: 1391 2298 2849 3179 3244 3452 1391 Size: 907 551 330 65 208 4072 Fragments arranged by size: 4072 907 551 330 208 65 BbeI G_GCGC'C Cuts at: 3335 3335 Size: 6133 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2446 928 Size: 971 547 4615 Fragments arranged by size: 4615 971 547 BbvCI CC'TCA_GC Cuts at: 2556 2556 Size: 6133 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2010 2044 3077 3111 4324 4358 1903 Size: 34 73 34 1033 34 1213 34 3678 Fragments arranged by size: 3678 1213 1033 73 34 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4025 5235 4025 Size: 1210 4923 BclI T'GATC_A Cuts at: 1548 1548 Size: 6133 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2853 3089 3248 Size: 107 122 71 631 605 1181 236 159 Cuts at: 3248 4744 136 Size: 1496 1525 Fragments arranged by size: 1525 1496 1181 631 605 236 159 122 107 71 BglII A'GATC_T Cuts at: 6128 6128 Size: 6133 BlpI GC'TnA_GC Cuts at: 3550 3550 Size: 6133 Bme1580I G_kGCm'C Cuts at: 1790 2533 2849 3244 4194 5350 1790 Size: 743 316 395 950 1156 2573 Fragments arranged by size: 2573 1156 950 743 395 316 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6133 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3864 4822 442 Size: 416 53 83 399 24 2447 958 1753 Fragments arranged by size: 2447 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6133 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2129 2174 2301 2376 2417 2433 2783 3065 Size: 890 45 127 75 41 16 350 282 Cuts at: 3065 4793 1239 Size: 1728 2579 Fragments arranged by size: 2579 1728 890 350 282 127 75 45 41 16 Bpu10I CC'TnA_GC Cuts at: 2459 2556 2783 3173 2459 Size: 97 227 390 5419 Fragments arranged by size: 5419 390 227 97 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3001 3272 4258 5123 5421 3001 Size: 271 986 865 298 3713 Fragments arranged by size: 3713 986 865 298 271 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 2639 4796 882 Size: 617 1140 2157 2219 Fragments arranged by size: 2219 2157 1140 617 BsaAI yAC'GTr Cuts at: 493 1204 3010 3924 493 Size: 711 1806 914 2702 Fragments arranged by size: 2702 1806 914 711 BsaBI GATnn'nnATC Cuts at: 2255 6127 2255 Size: 3872 2261 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2012 3332 3940 4322 Size: 53 83 186 936 479 1320 608 382 Cuts at: 4322 4957 275 Size: 635 1451 Fragments arranged by size: 1451 1320 936 635 608 479 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4566 4964 5238 5385 5796 4566 Size: 398 274 147 411 4903 Fragments arranged by size: 4903 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 2471 2501 2699 2729 3359 Size: 30 165 30 1068 30 198 30 630 Cuts at: 3359 3389 1178 Size: 30 3922 Fragments arranged by size: 3922 1068 630 198 165 30 30 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2234 2432 2435 2438 2720 1186 Size: 1048 198 3 3 282 4599 Fragments arranged by size: 4599 1048 282 198 3 3 BseYI C'CCAG_C Cuts at: 2087 2441 2823 3028 3067 3178 3268 5336 5832 Size: 354 382 205 39 111 90 2068 496 Cuts at: 5832 2087 Size: 2388 Fragments arranged by size: 2388 2068 496 382 354 205 111 90 39 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5560 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2389 3583 4344 5372 1540 Size: 275 186 388 1194 761 1028 2301 Fragments arranged by size: 2301 1194 1028 761 388 275 186 BsiHKAI G_wGCw'C Cuts at: 2613 4194 4279 5350 2613 Size: 1581 85 1071 3396 Fragments arranged by size: 3396 1581 1071 85 BsmI GAATG_Cn' Cuts at: 3644 3737 3644 Size: 93 6040 BsmBI CGTCTCn'nnnn_ Cuts at: 2003 2003 Size: 6133 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2298 2533 2613 2849 3179 3244 3452 Size: 399 508 235 80 236 330 65 208 Cuts at: 3452 4194 4279 5350 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 508 399 330 236 235 208 85 80 65 BspEI T'CCGG_A Cuts at: 5796 5796 Size: 6133 BspHI T'CATG_A Cuts at: 4022 5752 4022 Size: 1730 4403 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2722 2778 3399 3488 844 Size: 1878 56 621 89 3489 Fragments arranged by size: 3489 1878 621 89 56 BsrBI CCG'CTC Cuts at: 1394 1823 3064 4020 1394 Size: 429 1241 956 3507 Fragments arranged by size: 3507 1241 956 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4628 4802 65 Size: 1183 3380 174 1396 Fragments arranged by size: 3380 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 3328 4777 4964 1700 Size: 110 1518 1449 187 2869 Fragments arranged by size: 2869 1518 1449 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6133 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6133 BssSI C'ACGA_G Cuts at: 2470 4193 5205 2470 Size: 1723 1012 3398 Fragments arranged by size: 3398 1723 1012 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6133 BstBI TT'CG_AA Cuts at: 4937 4937 Size: 6133 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6133 BstYI r'GATC_y Cuts at: 1972 3039 3463 3830 4215 4232 5684 5770 5782 Size: 1067 424 367 385 17 1452 86 12 Cuts at: 5782 6128 1972 Size: 346 1977 Fragments arranged by size: 1977 1452 1067 424 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2337 2759 2337 Size: 422 5711 BtgI C'CryG_G Cuts at: 513 1065 2004 2383 513 Size: 552 939 379 4263 Fragments arranged by size: 4263 939 552 379 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5394 Fragments arranged by size: 5394 683 56 BtsI GCAGTG_nn' Cuts at: 3649 4443 4463 3649 Size: 794 20 5319 Fragments arranged by size: 5319 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6133 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6098 DraI TTT'AAA Cuts at: 3437 3437 Size: 6133 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5140 809 Size: 507 3824 1802 Fragments arranged by size: 3824 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2386 2511 3081 Size: 54 1425 50 275 18 556 125 570 Cuts at: 3081 3150 3219 3297 3580 4469 6098 8 Size: 69 69 78 283 889 1629 43 Fragments arranged by size: 1629 1425 889 570 556 283 275 125 78 69 69 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 2386 3580 1537 Size: 275 574 1194 4090 Fragments arranged by size: 4090 1194 574 275 EarI CTCTTCn'nnn_ Cuts at: 1353 2121 2556 2567 4063 1353 Size: 768 435 11 1496 3423 Fragments arranged by size: 3423 1496 768 435 11 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4720 5094 5240 387 Size: 1064 138 3131 374 146 1280 Fragments arranged by size: 3131 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2129 2174 2301 2376 2417 2433 2755 Size: 393 497 45 127 75 41 16 322 Cuts at: 2755 2783 3065 3095 4196 4793 5580 5848 1239 Size: 28 282 30 1101 597 787 268 1524 Fragments arranged by size: 1524 1101 787 597 497 393 322 282 268 127 75 45 41 30 28 16 EcoNI CCTnn'n_nnAGG Cuts at: 2120 2120 Size: 6133 EcoO109I rG'GnC_Cy Cuts at: 2058 2113 2140 2379 2764 2846 3241 3367 2058 Size: 55 27 239 385 82 395 126 4824 Fragments arranged by size: 4824 395 385 239 126 82 55 27 EcoRI G'AATT_C Cuts at: 2668 3442 3820 2668 Size: 774 378 4981 Fragments arranged by size: 4981 774 378 FspI TGC'GCA Cuts at: 4639 4639 Size: 6133 HaeII r_GCGC'y Cuts at: 1678 3205 3335 5280 1678 Size: 1527 130 1945 2531 Fragments arranged by size: 2531 1945 1527 130 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2471 2503 3130 3162 3359 Size: 32 135 32 899 32 627 32 197 Cuts at: 3359 3391 4774 4806 4848 4880 1373 Size: 32 1383 32 42 32 2626 Fragments arranged by size: 2626 1383 899 627 197 135 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3477 3723 669 Size: 988 281 1539 246 3079 Fragments arranged by size: 3079 1539 988 281 246 HindIII A'AGCT_T Cuts at: 748 3417 748 Size: 2669 3464 HpaI GTT'AAC Cuts at: 3723 3723 Size: 6133 KasI G'GCGC_C Cuts at: 3331 3331 Size: 6133 KpnI G_GTAC'C Cuts at: 3458 3458 Size: 6133 MfeI C'AATT_G Cuts at: 3732 3732 Size: 6133 MluI A'CGCG_T Cuts at: 6009 6009 Size: 6133 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2976 5247 5431 1305 Size: 1671 2271 184 2007 Fragments arranged by size: 2271 2007 1671 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2513 3083 3152 10 Size: 54 1425 343 681 570 69 2991 Fragments arranged by size: 2991 1425 681 570 343 69 54 MslI CAynn'nnrTG Cuts at: 518 1166 2357 2507 4091 4450 4609 518 Size: 648 1191 150 1584 359 159 2042 Fragments arranged by size: 2042 1584 1191 648 359 159 150 MspA1I CmG'CkG Cuts at: 1606 2110 2385 2437 2713 3095 3549 4226 5374 Size: 504 275 52 276 382 454 677 1148 Cuts at: 5374 5619 1606 Size: 245 2120 Fragments arranged by size: 2120 1148 677 504 454 382 276 275 245 52 NarI GG'CG_CC Cuts at: 3332 3332 Size: 6133 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4642 Fragments arranged by size: 4642 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6133 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6133 NotI GC'GGCC_GC Cuts at: 3580 3580 Size: 6133 NspI r_CATG'y Cuts at: 1132 1229 2348 3491 5036 1132 Size: 97 1119 1143 1545 2229 Fragments arranged by size: 2229 1545 1143 1119 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6133 PciI A'CATG_T Cuts at: 5032 5032 Size: 6133 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6133 PfoI T'CCnGG_A Cuts at: 2115 2493 3042 2115 Size: 378 549 5206 Fragments arranged by size: 5206 549 378 PmeI GTTT'AAAC Cuts at: 3437 3437 Size: 6133 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4202 4234 5715 5747 4202 Size: 32 1481 32 4588 Fragments arranged by size: 4588 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2113 2764 3367 2113 Size: 651 603 4879 Fragments arranged by size: 4879 651 603 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6133 PsiI TTA'TAA Cuts at: 3703 3703 Size: 6133 PspOMI G'GGCC_C Cuts at: 2845 3240 2845 Size: 395 5738 PspXI vC'TCGA_Gb Cuts at: 2883 2883 Size: 6133 PstI C_TGCA'G Cuts at: 830 3312 3399 3485 830 Size: 2482 87 86 3478 Fragments arranged by size: 3478 2482 87 86 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6133 PvuII CAG'CTG Cuts at: 2713 2713 Size: 6133 SacII CC_GC'GG Cuts at: 2386 2386 Size: 6133 SalI G'TCGA_C Cuts at: 1936 3475 1936 Size: 1539 4594 SanDI GG'GwC_CC Cuts at: 2764 2764 Size: 6133 SbfI CC_TGCA'GG Cuts at: 3485 3485 Size: 6133 ScaI AGT'ACT Cuts at: 4381 6093 4381 Size: 1712 4421 SfcI C'TryA_G Cuts at: 826 1045 3197 3308 3395 3481 4616 5297 5488 Size: 219 2152 111 87 86 1135 681 191 Cuts at: 5488 826 Size: 1471 Fragments arranged by size: 2152 1471 1135 681 219 191 111 87 86 SfiI GGCCn_nnn'nGGCC Cuts at: 3089 3248 3089 Size: 159 5974 SfoI GGC'GCC Cuts at: 3333 3333 Size: 6133 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6133 SgrAI Cr'CCGG_yG Cuts at: 1700 3328 1700 Size: 1628 4505 SmaI CCC'GGG Cuts at: 1872 2291 3460 1872 Size: 419 1169 4545 Fragments arranged by size: 4545 1169 419 SmlI C'TyrA_G Cuts at: 820 1017 1943 2883 2980 3287 4237 5138 5400 Size: 197 926 940 97 307 950 901 262 Cuts at: 5400 820 Size: 1553 Fragments arranged by size: 1553 950 940 926 901 307 262 197 97 SnaBI TAC'GTA Cuts at: 493 3924 493 Size: 3431 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6133 SphI G_CATG'C Cuts at: 1132 2348 3491 1132 Size: 1216 1143 3774 Fragments arranged by size: 3774 1216 1143 SspI AAT'ATT Cuts at: 5 52 4057 5 Size: 47 4005 2081 Fragments arranged by size: 4005 2081 47 StuI AGG'CCT Cuts at: 1517 2394 3171 1517 Size: 877 777 4479 Fragments arranged by size: 4479 877 777 StyI C'CwwG_G Cuts at: 513 1065 2004 2274 2514 3572 513 Size: 552 939 270 240 1058 3074 Fragments arranged by size: 3074 1058 939 552 270 240 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2879 3296 4160 4177 4330 4515 4960 Size: 1255 1299 417 864 17 153 185 445 Cuts at: 4960 325 Size: 1498 Fragments arranged by size: 1498 1299 1255 864 445 417 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2655 2679 3228 Size: 274 80 33 51 1443 678 24 549 Cuts at: 3228 4379 6091 96 Size: 1151 1712 138 Fragments arranged by size: 1712 1443 1151 678 549 274 138 80 51 33 24 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 6093 6125 6093 Size: 32 6101 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6133 XbaI T'CTAG_A Cuts at: 3469 3469 Size: 6133 XhoI C'TCGA_G Cuts at: 1943 2883 1943 Size: 940 5193 XmaI C'CCGG_G Cuts at: 1870 2289 3458 1870 Size: 419 1169 4545 Fragments arranged by size: 4545 1169 419 XmnI GAAnn'nnTTC Cuts at: 4262 4262 Size: 6133 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3941 276 Size: 53 83 186 936 2407 2468 Fragments arranged by size: 2468 2407 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BbvCI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NarI NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PspOMI PspXI PstI PvuI PvuII SacII SalI SanDI SbfI ScaI SfcI SfiI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BfrBI BplI BsiWI BstXI BstZ17I DraIII EcoICRI EcoRV FalI FseI FspAI NaeI NgoMIV NruI NsiI PacI PmlI PsrI RsrII SacI SapI SexAI SrfI SwaI XcmI