Restriction map for
pFN21AB8717
(Circular) (Six-base) MAPSORT of: pFN21AB8717.seq Check: 6594 from: 1 to: 6148
pFN21AB8717 6148 bp
With 182 enzymes: SgfI *
January 16, 2009 15:27 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 2925 2925
Size: 6148
AatII G_ACGT'C
Cuts at: 278 331 414 600 1536 3958 278
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
AccI GT'mk_AC
Cuts at: 1937 2638 3491 1937
Size: 701 853 4594
Fragments arranged by size:
4594 853 701
Acc65I G'GTAC_C
Cuts at: 3469 3469
Size: 6148
AclI AA'CG_TT
Cuts at: 1563 2382 4275 4648 1563
Size: 819 1893 373 3063
Fragments arranged by size:
3063 1893 819 373
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 1632 2464 2810 4211 5595 5863 1632
Size: 832 346 1401 1384 268 1917
Fragments arranged by size:
1917 1401 1384 832 346 268
AflII C'TTAA_G
Cuts at: 820 1017 2272 820
Size: 197 1255 4696
Fragments arranged by size:
4696 1255 197
AflIII A'CryG_T
Cuts at: 1203 5047 6024 1203
Size: 3844 977 1327
Fragments arranged by size:
3844 1327 977
AgeI A'CCGG_T
Cuts at: 4979 4979
Size: 6148
AhdI GACnn_n'nnGTC
Cuts at: 2401 4877 2401
Size: 2476 3672
AleI CACnn'nnGTG
Cuts at: 1166 3191 1166
Size: 2025 4123
AlfI GCAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1184 1184
Size: 6148
AlwNI CAG_nnn'CTG
Cuts at: 2200 3118 3891 5463 2200
Size: 918 773 1572 2885
Fragments arranged by size:
2885 1572 918 773
ApaLI G'TGCA_C
Cuts at: 4205 5361 4205
Size: 1156 4992
ApoI r'AATT_y
Cuts at: 1493 3457 3683 3835 5944 1493
Size: 1964 226 152 2109 1697
Fragments arranged by size:
2109 1964 1697 226 152
AseI AT'TA_AT
Cuts at: 160 1032 4702 160
Size: 872 3670 1606
Fragments arranged by size:
3670 1606 872
AsiSI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
AvaI C'yCGr_G
Cuts at: 1870 1943 2671 3189 3302 3473 1870
Size: 73 728 518 113 171 4545
Fragments arranged by size:
4545 728 518 171 113 73
BamHI G'GATC_C
Cuts at: 2812 3478 3845 2812
Size: 666 367 5115
Fragments arranged by size:
5115 666 367
BanI G'GyrC_C
Cuts at: 618 943 1160 1787 3469 4924 618
Size: 325 217 627 1682 1455 1842
Fragments arranged by size:
1842 1682 1455 627 325 217
BanII G_rGCy'C
Cuts at: 1391 3411 3467 1391
Size: 2020 56 4072
Fragments arranged by size:
4072 2020 56
BbsI GAAGACnn'nnnn_
Cuts at: 928 1899 928
Size: 971 5177
BbvCI CC'TCA_GC
Cuts at: 3402 3402
Size: 6148
BcgI CGAnnnnnnTGCnnnnnnnnnn_nn'
Cuts at: 1903 1937 2116 2150 4339 4373 1903
Size: 34 179 34 2189 34 3678
Fragments arranged by size:
3678 2189 179 34 34 34
BciVI GTATCCnnnnn_n'
Cuts at: 2192 3008 4040 5250 2192
Size: 816 1032 1210 3090
Fragments arranged by size:
3090 1210 1032 816
BclI T'GATC_A
Cuts at: 1548 1548
Size: 6148
BfrBI ATG'CAT
Cuts at: 2738 2738
Size: 6148
BglI GCCn_nnn'nGGC
Cuts at: 136 243 365 436 1067 1672 4759 136
Size: 107 122 71 631 605 3087 1525
Fragments arranged by size:
3087 1525 631 605 122 107 71
BglII A'GATC_T
Cuts at: 2215 6143 2215
Size: 3928 2220
BlpI GC'TnA_GC
Cuts at: 3565 3565
Size: 6148
Bme1580I G_kGCm'C
Cuts at: 1790 4209 5365 1790
Size: 2419 1156 2573
Fragments arranged by size:
2573 2419 1156
BmgBI CAC'GTC
Cuts at: 1318 2071 1318
Size: 753 5395
BmrI ACTGGGnnnn_n'
Cuts at: 442 858 911 994 1393 1417 3417 3879 4837
Size: 416 53 83 399 24 2000 462 958
Cuts at: 4837 442
Size: 1753
Fragments arranged by size:
2000 1753 958 462 416 399 83 53
24
BmtI G_CTAG'C
Cuts at: 1056 1056
Size: 6148
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 3427 3427
Size: 6148
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 2047 2210 2912 3202 4808 1239
Size: 808 163 702 290 1606 2579
Fragments arranged by size:
2579 1606 808 702 290 163
Bpu10I CC'TnA_GC
Cuts at: 2090 3267 3402 2090
Size: 1177 135 4836
Fragments arranged by size:
4836 1177 135
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 2681 4273 5138 5436 2681
Size: 1592 865 298 3393
Fragments arranged by size:
3393 1592 865 298
BsaI GGTCTCn'nnnn_
Cuts at: 882 1499 2300 3314 4811 882
Size: 617 801 1014 1497 2219
Fragments arranged by size:
2219 1497 1014 801 617
BsaAI yAC'GTr
Cuts at: 493 1204 3939 493
Size: 711 2735 2702
Fragments arranged by size:
2735 2702 711
BsaBI GATnn'nnATC
Cuts at: 6142 6142
Size: 6148
BsaHI Gr'CG_yC
Cuts at: 275 328 411 597 1533 2169 3955 4337 4972
Size: 53 83 186 936 636 1786 382 635
Cuts at: 4972 275
Size: 1451
Fragments arranged by size:
1786 1451 936 636 635 382 186 83
53
BsaWI w'CCGG_w
Cuts at: 2177 4581 4979 5253 5400 5811 2177
Size: 2404 398 274 147 411 2514
Fragments arranged by size:
2514 2404 411 398 274 147
BsaXI ACnnnnnCTCCnnnnnnn_nnn'
Cuts at: 1178 1208 1373 1403 1178
Size: 30 165 30 5923
Fragments arranged by size:
5923 165 30 30
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 1186 2102 2520 1186
Size: 916 418 4814
Fragments arranged by size:
4814 916 418
BseYI C'CCAG_C
Cuts at: 2704 2778 2831 2835 5351 5847 2704
Size: 74 53 4 2516 496 3005
Fragments arranged by size:
3005 2516 496 74 53 4
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 1163 1736 2632 1163
Size: 573 896 4679
Fragments arranged by size:
4679 896 573
BsiEI CG_ry'CG
Cuts at: 1540 1815 2001 2148 3598 4359 5387 1540
Size: 275 186 147 1450 761 1028 2301
Fragments arranged by size:
2301 1450 1028 761 275 186 147
BsiHKAI G_wGCw'C
Cuts at: 2100 4209 4294 5365 2100
Size: 2109 85 1071 2883
Fragments arranged by size:
2883 2109 1071 85
BsmI GAATG_Cn'
Cuts at: 2738 3659 3752 2738
Size: 921 93 5134
Fragments arranged by size:
5134 921 93
BsmBI CGTCTCn'nnnn_
Cuts at: 2927 2927
Size: 6148
Bsp1286I G_dGCh'C
Cuts at: 1391 1790 2100 3411 3467 4209 4294 5365 1391
Size: 399 310 1311 56 742 85 1071 2174
Fragments arranged by size:
2174 1311 1071 742 399 310 85 56
BspEI T'CCGG_A
Cuts at: 5811 5811
Size: 6148
BspHI T'CATG_A
Cuts at: 4037 5767 4037
Size: 1730 4418
BspMI ACCTGCnnnn'nnnn_
Cuts at: 844 2194 2925 3400 3503 844
Size: 1350 731 475 103 3489
Fragments arranged by size:
3489 1350 731 475 103
BsrBI CCG'CTC
Cuts at: 1394 1823 4035 1394
Size: 429 2212 3507
Fragments arranged by size:
3507 2212 429
BsrDI GCAATG_nn'
Cuts at: 65 1248 3099 4643 4817 65
Size: 1183 1851 1544 174 1396
Fragments arranged by size:
1851 1544 1396 1183 174
BsrFI r'CCGG_y
Cuts at: 1700 1810 4792 4979 1700
Size: 110 2982 187 2869
Fragments arranged by size:
2982 2869 187 110
BsrGI T'GTAC_A
Cuts at: 96 96
Size: 6148
BssHII G'CGCG_C
Cuts at: 1925 2123 1925
Size: 198 5950
BssSI C'ACGA_G
Cuts at: 4208 5220 4208
Size: 1012 5136
BstAPI GCAn_nnn'nTGC
Cuts at: 794 2200 3030 794
Size: 1406 830 3912
Fragments arranged by size:
3912 1406 830
BstBI TT'CG_AA
Cuts at: 4952 4952
Size: 6148
BstEII G'GTnAC_C
Cuts at: 1184 3169 1184
Size: 1985 4163
BstYI r'GATC_y
Cuts at: 1972 2215 2692 2812 3478 3845 4230 4247 5699
Size: 243 477 120 666 367 385 17 1452
Cuts at: 5699 5785 5797 6143 1972
Size: 86 12 346 1977
Fragments arranged by size:
1977 1452 666 477 385 367 346 243
120 86 17 12
Bsu36I CC'TnA_GG
Cuts at: 2817 3353 2817
Size: 536 5612
BtgI C'CryG_G
Cuts at: 513 1065 3341 513
Size: 552 2276 3320
Fragments arranged by size:
3320 2276 552
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 487 1170 1226 487
Size: 683 56 5409
Fragments arranged by size:
5409 683 56
BtsI GCAGTG_nn'
Cuts at: 2158 3170 3664 4458 4478 2158
Size: 1012 494 794 20 3828
Fragments arranged by size:
3828 1012 794 494 20
ClaI AT'CG_AT
Cuts at: 1554 1554
Size: 6148
CspCI CAAnnnnnGTGGnnnnnnnnnn_nn'
Cuts at: 529 564 529
Size: 35 6113
DraI TTT'AAA
Cuts at: 3452 3452
Size: 6148
DraIII CAC_nnn'GTG
Cuts at: 2990 2990
Size: 6148
DrdI GACnn_nn'nnGTC
Cuts at: 809 1316 5155 809
Size: 507 3839 1802
Fragments arranged by size:
3839 1802 507
EaeI y'GGCC_r
Cuts at: 8 62 1487 1537 1812 1830 2145 2313 3595
Size: 54 1425 50 275 18 315 168 1282
Cuts at: 3595 4484 6113 8
Size: 889 1629 43
Fragments arranged by size:
1629 1425 1282 889 315 275 168 54
50 43 18
EagI C'GGCC_G
Cuts at: 1537 1812 2145 3595 1537
Size: 275 333 1450 4090
Fragments arranged by size:
4090 1450 333 275
EarI CTCTTCn'nnn_
Cuts at: 1353 2748 3434 4078 1353
Size: 1395 686 644 3423
Fragments arranged by size:
3423 1395 686 644
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 387 1451 1589 4735 5109 5255 387
Size: 1064 138 3146 374 146 1280
Fragments arranged by size:
3146 1280 1064 374 146 138
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 1239 1632 2047 2210 2464 2810 2912 3202 4211
Size: 393 415 163 254 346 102 290 1009
Cuts at: 4211 4808 5595 5863 1239
Size: 597 787 268 1524
Fragments arranged by size:
1524 1009 787 597 415 393 346 290
268 254 163 102
EcoNI CCTnn'n_nnAGG
Cuts at: 2288 2288
Size: 6148
EcoO109I rG'GnC_Cy
Cuts at: 2206 2775 3222 2206
Size: 569 447 5132
Fragments arranged by size:
5132 569 447
EcoRI G'AATT_C
Cuts at: 3457 3835 3457
Size: 378 5770
FspI TGC'GCA
Cuts at: 4654 4654
Size: 6148
HaeII r_GCGC'y
Cuts at: 1678 2142 5295 1678
Size: 464 3153 2531
Fragments arranged by size:
3153 2531 464
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 1373 1405 1540 1572 2280 2312 2387 2419 2667
Size: 32 135 32 708 32 75 32 248
Cuts at: 2667 2699 2948 2980 4789 4821 4863 4895 1373
Size: 32 249 32 1809 32 42 32 2626
Fragments arranged by size:
2626 1809 708 249 248 135 75 42
32 32 32 32 32 32 32 32
HincII GTy'rAC
Cuts at: 669 1657 1938 3492 3738 669
Size: 988 281 1554 246 3079
Fragments arranged by size:
3079 1554 988 281 246
HindIII A'AGCT_T
Cuts at: 748 748
Size: 6148
HpaI GTT'AAC
Cuts at: 3738 3738
Size: 6148
KpnI G_GTAC'C
Cuts at: 3473 3473
Size: 6148
MfeI C'AATT_G
Cuts at: 3747 3747
Size: 6148
MluI A'CGCG_T
Cuts at: 6024 6024
Size: 6148
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 1305 2219 2311 5262 5446 1305
Size: 914 92 2951 184 2007
Fragments arranged by size:
2951 2007 914 184 92
MscI TGG'CCA
Cuts at: 10 64 1489 1832 10
Size: 54 1425 343 4326
Fragments arranged by size:
4326 1425 343 54
MslI CAynn'nnrTG
Cuts at: 518 1166 2552 3191 4106 4465 4624 518
Size: 648 1386 639 915 359 159 2042
Fragments arranged by size:
2042 1386 915 648 639 359 159
MspA1I CmG'CkG
Cuts at: 1606 2085 2188 2835 3343 3564 4241 5389 5634
Size: 479 103 647 508 221 677 1148 245
Cuts at: 5634 1606
Size: 2120
Fragments arranged by size:
2120 1148 677 647 508 479 245 221
103
NcoI C'CATG_G
Cuts at: 513 1065 513
Size: 552 5596
NdeI CA'TA_TG
Cuts at: 387 387
Size: 6148
NheI G'CTAG_C
Cuts at: 1052 1052
Size: 6148
NotI GC'GGCC_GC
Cuts at: 3595 3595
Size: 6148
NsiI A_TGCA'T
Cuts at: 2740 2740
Size: 6148
NspI r_CATG'y
Cuts at: 1132 1229 2131 3506 5051 1132
Size: 97 902 1375 1545 2229
Fragments arranged by size:
2229 1545 1375 902 97
PasI CC'CwG_GG
Cuts at: 1348 2937 1348
Size: 1589 4559
PciI A'CATG_T
Cuts at: 5047 5047
Size: 6148
PflMI CCAn_nnn'nTGG
Cuts at: 1108 3118 1108
Size: 2010 4138
PfoI T'CCnGG_A
Cuts at: 2952 2952
Size: 6148
PmeI GTTT'AAAC
Cuts at: 3452 3452
Size: 6148
PpiI GAACnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 4217 4249 5730 5762 4217
Size: 32 1481 32 4603
Fragments arranged by size:
4603 1481 32 32
PpuMI rG'GwC_Cy
Cuts at: 2206 3222 2206
Size: 1016 5132
PshAI GACnn'nnGTC
Cuts at: 1613 1613
Size: 6148
PsiI TTA'TAA
Cuts at: 3718 3718
Size: 6148
PspXI vC'TCGA_Gb
Cuts at: 3189 3189
Size: 6148
PstI C_TGCA'G
Cuts at: 830 2205 3500 830
Size: 1375 1295 3478
Fragments arranged by size:
3478 1375 1295
PvuI CG_AT'CG
Cuts at: 2001 2001
Size: 6148
PvuII CAG'CTG
Cuts at: 2835 2835
Size: 6148
SacII CC_GC'GG
Cuts at: 3344 3344
Size: 6148
SalI G'TCGA_C
Cuts at: 1936 3490 1936
Size: 1554 4594
SbfI CC_TGCA'GG
Cuts at: 3500 3500
Size: 6148
ScaI AGT'ACT
Cuts at: 4396 6108 4396
Size: 1712 4436
SfcI C'TryA_G
Cuts at: 826 1045 2201 2455 2979 3496 4631 5312 5503
Size: 219 1156 254 524 517 1135 681 191
Cuts at: 5503 826
Size: 1471
Fragments arranged by size:
1471 1156 1135 681 524 517 254 219
191
SgfI GCG_AT'CGC
Cuts at: 2001 2001
Size: 6148
SgrAI Cr'CCGG_yG
Cuts at: 1700 1700
Size: 6148
SmaI CCC'GGG
Cuts at: 1872 3475 1872
Size: 1603 4545
SmlI C'TyrA_G
Cuts at: 820 1017 1943 2272 2671 2696 3189 4252 5153
Size: 197 926 329 399 25 493 1063 901
Cuts at: 5153 5415 820
Size: 262 1553
Fragments arranged by size:
1553 1063 926 901 493 399 329 262
197 25
SnaBI TAC'GTA
Cuts at: 493 3939 493
Size: 3446 2702
SpeI A'CTAG_T
Cuts at: 152 152
Size: 6148
SphI G_CATG'C
Cuts at: 1132 3506 1132
Size: 2374 3774
SspI AAT'ATT
Cuts at: 5 52 4072 5
Size: 47 4020 2081
Fragments arranged by size:
4020 2081 47
StuI AGG'CCT
Cuts at: 1517 2787 3251 1517
Size: 1270 464 4414
Fragments arranged by size:
4414 1270 464
StyI C'CwwG_G
Cuts at: 513 1065 2427 2877 3587 513
Size: 552 1362 450 710 3074
Fragments arranged by size:
3074 1362 710 552 450
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 325 1580 2391 3157 4175 4192 4345 4530 4975
Size: 1255 811 766 1018 17 153 185 445
Cuts at: 4975 325
Size: 1498
Fragments arranged by size:
1498 1255 1018 811 766 445 185 153
17
TatI w'GTAC_w
Cuts at: 96 370 450 483 534 1977 4394 6106 96
Size: 274 80 33 51 1443 2417 1712 138
Fragments arranged by size:
2417 1712 1443 274 138 80 51 33
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 6108 6140 6108
Size: 32 6116
Tth111I GACn'n_nGTC
Cuts at: 1316 2292 2414 3149 1316
Size: 976 122 735 4315
Fragments arranged by size:
4315 976 735 122
XbaI T'CTAG_A
Cuts at: 3484 3484
Size: 6148
XhoI C'TCGA_G
Cuts at: 1943 2671 3189 1943
Size: 728 518 4902
Fragments arranged by size:
4902 728 518
XmaI C'CCGG_G
Cuts at: 1870 3473 1870
Size: 1603 4545
XmnI GAAnn'nnTTC
Cuts at: 2382 3371 4277 2382
Size: 989 906 4253
Fragments arranged by size:
4253 989 906
ZraI GAC'GTC
Cuts at: 276 329 412 598 1534 3956 276
Size: 53 83 186 936 2422 2468
Fragments arranged by size:
2468 2422 936 186 83 53
Enzymes that do cut:
AarI AatII AccI Acc65I AclI AcuI AflII AflIII
AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI
AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI
BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI
BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI
BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI
BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI
BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII
BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI
DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoNI
EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI
KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI
NdeI NheI NotI NsiI NspI PasI PciI PflMI
PfoI PmeI PpiI PpuMI PshAI PsiI PspXI PstI
PvuI PvuII SacII SalI SbfI ScaI SfcI SgfI
SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI
StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI
XmnI ZraI
Enzymes that do not cut:
AfeI AloI ApaI AscI AvrII BaeI BbeI BsiWI
BstXI BstZ17I EcoICRI EcoRV FalI FseI FspAI KasI
NaeI NarI NgoMIV NruI PacI PmlI PspOMI PsrI
RsrII SacI SanDI SapI SexAI SfiI SfoI SrfI
SwaI XcmI