Restriction map for
pFN21AB8716
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# NO CUT ENZYMES ( 115 enzymes) #
##################################
AatII Acc65I AccI AccIII AclI
AcsI AflII AflIII AgeI AhdI
AocI Aor51HI ApoI AscI AseI
AsnI Asp718I AspEI AspI AviII
AvrII BbvCI BcgI BciVI BclI
BfaI BfrI BglI BglII BlnI
BsaI BsaWI BseAI BsiWI BsmAI
BsmBI BsmI Bsp1407I BspDI BspEI
BspLU11I BsrDI BsrGI BssSI Bst1107I
BstAPI BstBI BstEII BstPI BstXI
BstZ17I Bsu36I ClaI CpoI DraI
Eam1105I Eco47III Eco81I EcoO65I EcoRI
EcoRV EcoT22I FbaI FseI FspI
HindIII HpaI KpnI MaeI MfeI
MluI MroI MseI MunI NcoI
NdeI NheI NruI NsiI NspI
NspV PacI PaeR7I PflFI PflMI
PinAI PmeI PshAI PshBI Psp1406I
PvuI RsrII SalI SbfI SexAI
SfiI SfuI SgrAI SmlI SnaBI
SpeI SphI Sse8387I SspBI SspI
StuI SwaI TfiI Tru9I Tsp45I
Tsp509I Tth111I Van91I XbaI XhoI
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# ONE CUT ENZYMES ( 31 enzymes) #
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BamHI 893 GGATCC G^GATCC
BbrPI 289 CACGTG CAC^GTG
BbsI 993 GAAGAC GAAGAC(2/6)
BlpI 306 GCTGAGC GC^T[ACGT]AGC
BpmI 238 CTGGAG CTGGAG(16/14)
Bpu1102I 306 GCTGAGC GC^T[ACGT]AGC
BpuAI 993 GAAGAC GAAGAC(2/6)
BsaBI 1575 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
BspHI 497 TCATGA T^CATGA
BsrI 138 CCAGT ACTGG(1/-1)
CelII 306 GCTGAGC GC^T[ACGT]AGC
DraIII 574 CACAAGGTG CAC[ACGT][ACGT][ACGT]^GTG
DrdI 724 GACAACGTGGTC GAC[ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GTC
HincII 517 GTCAAC GT[CT]^[GA]AC
HindII 517 GTCAAC GT[CT]^[GA]AC
HinfI 793 GACTC G^A[ACGT]TC
MaeIII 89 GTTAC ^GT[ACGT]AC
MamI 1575 GATCCTCATC GAT[ACGT][ACGT]^[ACGT][ACGT]ATC
MslI 945 CATGCGCGTG CA[CT][ACGT][ACGT]^[ACGT][ACGT][GA]TG
NaeI 596 GCCGGC GCC^GGC
NgoMIV 596 GCCGGC G^CCGGC
PleI 793 GACTC GAGTC(4/5)
PmaCI 289 CACGTG CAC^GTG
PmlI 289 CACGTG CAC^GTG
PpuMI 1544 GGGACCT [GA]G^G[AT]CC[CT]
PvuII 739 CAGCTG CAG^CTG
RcaI 497 TCATGA T^CATGA
SacI 55 GAGCTC GAGCT^C
SapI 204 GAAGAGC GCTCTTC(1/4)
ScaI 1615 AGTACT AGT^ACT
TspRI 601 CAGTG CA[GC]TG[ACGT][ACGT]^
###################################
# TWO CUT ENZYMES ( 21 enzymes) #
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Alw44I 333 GTGCAC G^TGCAC
1147 GTGCAC
AlwNI 217 CAGAACCTG CAG[ACGT][ACGT][ACGT]^CTG
739 CAGCTGCTG
ApaLI 333 GTGCAC G^TGCAC
1147 GTGCAC
Asp700I 219 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
360 GAACGAGTTC
BsaAI 4 TACGTG [CT]AC^GT[GA]
289 CACGTG
BseRI 1504 GAGGAG GAGGAG(10/8)
1507 GAGGAG
BspMI 370 ACCTGC ACCTGC(4/8)
403 GCAGGT
BsrFI 596 GCCGGC [GA]^CCGG[CT]
683 ACCGGC
Cfr10I 596 GCCGGC [GA]^CCGG[CT]
683 ACCGGC
DdeI 307 CTGAG C^T[ACGT]AG
1447 CTGAG
EarI 532 CTCTTC CTCTTC(1/4)
204 GAAGAG
EcoT14I 143 CCAAGG C^C[AT][AT]GG
1178 CCAAGG
Ksp632I 532 CTCTTC CTCTTC(1/4)
204 GAAGAG
KspI 246 CCGCGG CCGC^GG
416 CCGCGG
NotI 248 GCGGCCGC GC^GGCCGC
870 GCGGCCGC
PstI 70 CTGCAG CTGCA^G
454 CTGCAG
SacII 246 CCGCGG CCGC^GG
416 CCGCGG
SfcI 70 CTGCAG C^T[GA][CT]AG
454 CTGCAG
StyI 143 CCAAGG C^C[AT][AT]GG
1178 CCAAGG
XcmI 269 CCAAGCTGTACCTGG CCA[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT][ACGT][ACGT]TGG
941 CCATCATGCGCGTGG
XmnI 219 GAACCTGTTC GAA[ACGT][ACGT]^[ACGT][ACGT]TTC
360 GAACGAGTTC
#########################################
# 3 OR MORE CUT ENZYMES ( 91 enzymes) #
#########################################
AccII AciI AcyI AfaI AluI
AlwI ApaI AspHI AvaI AvaII
BalI BanI BanII BbeI BbvI
BcnI BmyI BsaHI BsaJI BsgI
BsiEI BsiHKAI BsiYI BslI BsmFI
BsoBI Bsp1286I BsrBI BssHII BssKI
BstF5I BstNI BstUI BstYI Cac8I
CfoI Cfr13I DpnII DraII DsaI
EaeI EagI EclXI Eco52I EcoNI
EcoO109I EcoRII FauI Fnu4HI FokI
HaeII HaeIII HapII HgaI HhaI
Hin1I HinP1I HpaII HphI ItaI
KasI MaeII MboI MboII MflI
MluNI MnlI MscI MspA1I MspI
MvaI MvnI MwoI NarI NciI
NdeII NlaIII NlaIV RsaI Sau3AI
Sau96I ScrFI SfaNI SfoI SmaI
TaqI TseI TthHB8I XhoII XmaCI
XmaI