(Circular) (Six-base) MAPSORT of: pFN21AB8716.seq Check: 8848 from: 1 to: 6334 pFN21AB8716 6334 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2384 2399 2384 Size: 15 6319 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 4144 278 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3677 1937 Size: 1740 4594 Acc65I G'GTAC_C Cuts at: 3655 3655 Size: 6334 AclI AA'CG_TT Cuts at: 1563 4461 4834 1563 Size: 2898 373 3063 Fragments arranged by size: 3063 2898 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 4397 5781 6049 1632 Size: 2765 1384 268 1917 Fragments arranged by size: 2765 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 6137 AflIII A'CryG_T Cuts at: 1203 5233 6210 1203 Size: 4030 977 1327 Fragments arranged by size: 4030 1327 977 AgeI A'CCGG_T Cuts at: 5165 5165 Size: 6334 AhdI GACnn_n'nnGTC Cuts at: 5063 5063 Size: 6334 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6334 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6334 AlwNI CAG_nnn'CTG Cuts at: 2227 2749 4077 5649 2227 Size: 522 1328 1572 2912 Fragments arranged by size: 2912 1572 1328 522 ApaI G_GGCC'C Cuts at: 2156 2421 3080 3365 3527 2156 Size: 265 659 285 162 4963 Fragments arranged by size: 4963 659 285 265 162 ApaLI G'TGCA_C Cuts at: 2338 3152 4391 5547 2338 Size: 814 1239 1156 3125 Fragments arranged by size: 3125 1239 1156 814 ApoI r'AATT_y Cuts at: 1493 3643 3869 4021 6130 1493 Size: 2150 226 152 2109 1697 Fragments arranged by size: 2150 2109 1697 226 152 AseI AT'TA_AT Cuts at: 160 1032 4888 160 Size: 872 3856 1606 Fragments arranged by size: 3856 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 AvaI C'yCGr_G Cuts at: 1870 1943 2155 2800 3073 3305 3339 3355 3504 Size: 73 212 645 273 232 34 16 149 Cuts at: 3504 3659 1870 Size: 155 4545 Fragments arranged by size: 4545 645 273 232 212 155 149 73 34 16 BamHI G'GATC_C Cuts at: 2898 3664 4031 2898 Size: 766 367 5201 Fragments arranged by size: 5201 766 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2610 2849 3170 3196 3280 Size: 325 217 627 823 239 321 26 84 Cuts at: 3280 3655 5110 618 Size: 375 1455 1842 Fragments arranged by size: 1842 1455 823 627 375 325 321 239 217 84 26 BanII G_rGCy'C Cuts at: 1391 2064 2156 2216 2421 2844 3080 3365 3441 Size: 673 92 60 205 423 236 285 76 Cuts at: 3441 3527 3653 1391 Size: 86 126 4072 Fragments arranged by size: 4072 673 423 285 236 205 126 92 86 76 60 BbeI G_GCGC'C Cuts at: 2614 2853 3284 2614 Size: 239 431 5664 Fragments arranged by size: 5664 431 239 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 3005 928 Size: 971 1106 4257 Fragments arranged by size: 4257 1106 971 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4525 4559 1903 Size: 34 2588 34 3678 Fragments arranged by size: 3678 2588 34 34 BciVI GTATCCnnnnn_n' Cuts at: 4226 5436 4226 Size: 1210 5124 BclI T'GATC_A Cuts at: 1548 1548 Size: 6334 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4945 136 Size: 107 122 71 631 605 3273 1525 Fragments arranged by size: 3273 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6329 6329 Size: 6334 BlpI GC'TnA_GC Cuts at: 2312 3751 2312 Size: 1439 4895 Bme1580I G_kGCm'C Cuts at: 1790 2016 2156 2325 2342 2421 3080 3156 3201 Size: 226 140 169 17 79 659 76 45 Cuts at: 3201 3365 3527 4395 5551 1790 Size: 164 162 868 1156 2573 Fragments arranged by size: 2573 1156 868 659 226 169 164 162 140 79 76 45 17 BmgBI CAC'GTC Cuts at: 1318 3151 3373 3544 1318 Size: 1833 222 171 4108 Fragments arranged by size: 4108 1833 222 171 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 4065 5023 442 Size: 416 53 83 399 24 2648 958 1753 Fragments arranged by size: 2648 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6334 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2264 4994 1239 Size: 1025 2730 2579 Fragments arranged by size: 2730 2579 1025 Bpu10I CC'TnA_GC Cuts at: 3452 3452 Size: 6334 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4459 5324 5622 4459 Size: 865 298 5171 Fragments arranged by size: 5171 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4997 882 Size: 617 3498 2219 Fragments arranged by size: 3498 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2011 2296 4125 493 Size: 711 807 285 1829 2702 Fragments arranged by size: 2702 1829 807 711 285 BsaBI GATnn'nnATC Cuts at: 3584 6328 3584 Size: 2744 3590 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2611 2778 2850 3281 Size: 53 83 186 936 1078 167 72 431 Cuts at: 3281 4141 4523 5158 275 Size: 860 382 635 1451 Fragments arranged by size: 1451 1078 936 860 635 431 382 186 167 83 72 53 BsaWI w'CCGG_w Cuts at: 4767 5165 5439 5586 5997 4767 Size: 398 274 147 411 5104 Fragments arranged by size: 5104 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 6109 Fragments arranged by size: 6109 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 3524 3527 1186 Size: 2338 3 3993 Fragments arranged by size: 3993 2338 3 BseYI C'CCAG_C Cuts at: 2020 2025 2383 2822 3265 5537 6033 2020 Size: 5 358 439 443 2272 496 2321 Fragments arranged by size: 2321 2272 496 443 439 358 5 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2561 2828 3464 1163 Size: 573 825 267 636 4033 Fragments arranged by size: 4033 825 636 573 267 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 2109 2257 2879 3615 3784 4545 Size: 275 186 108 148 622 736 169 761 Cuts at: 4545 5573 1540 Size: 1028 2301 Fragments arranged by size: 2301 1028 761 736 622 275 186 169 148 108 BsiHKAI G_wGCw'C Cuts at: 2064 2342 3156 4395 4480 5551 2064 Size: 278 814 1239 85 1071 2847 Fragments arranged by size: 2847 1239 1071 814 278 85 BsmI GAATG_Cn' Cuts at: 3845 3938 3845 Size: 93 6241 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2016 2064 2156 2216 2325 2342 2421 Size: 399 226 48 92 60 109 17 79 Cuts at: 2421 2844 3080 3156 3201 3365 3441 3527 3653 Size: 423 236 76 45 164 76 86 126 Cuts at: 3653 4395 4480 5551 1391 Size: 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 423 399 236 226 164 126 109 92 86 85 79 76 76 60 48 45 17 BspEI T'CCGG_A Cuts at: 5997 5997 Size: 6334 BspHI T'CATG_A Cuts at: 2502 4223 5953 2502 Size: 1721 1730 2883 Fragments arranged by size: 2883 1730 1721 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2384 2399 3689 844 Size: 1540 15 1290 3489 Fragments arranged by size: 3489 1540 1290 15 BsrBI CCG'CTC Cuts at: 1394 1823 2616 3039 3301 3378 4221 1394 Size: 429 793 423 262 77 843 3507 Fragments arranged by size: 3507 843 793 429 423 262 77 BsrDI GCAATG_nn' Cuts at: 65 1248 4829 5003 65 Size: 1183 3581 174 1396 Fragments arranged by size: 3581 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2601 2688 4978 5165 1700 Size: 110 791 87 2290 187 2869 Fragments arranged by size: 2869 2290 791 187 110 87 BsrGI T'GTAC_A Cuts at: 96 96 Size: 6334 BssHII G'CGCG_C Cuts at: 1925 2118 2379 2990 3131 1925 Size: 193 261 611 141 5128 Fragments arranged by size: 5128 611 261 193 141 BssSI C'ACGA_G Cuts at: 4394 5406 4394 Size: 1012 5322 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6334 BstBI TT'CG_AA Cuts at: 5138 5138 Size: 6334 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6334 BstYI r'GATC_y Cuts at: 1972 2139 2898 3579 3664 4031 4416 4433 5885 Size: 167 759 681 85 367 385 17 1452 Cuts at: 5885 5971 5983 6329 1972 Size: 86 12 346 1977 Fragments arranged by size: 1977 1452 759 681 385 367 346 167 86 85 17 12 BtgI C'CryG_G Cuts at: 513 1065 2251 2421 2787 2814 2865 3006 513 Size: 552 1186 170 366 27 51 141 3841 Fragments arranged by size: 3841 1186 552 366 170 141 51 27 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2455 3541 3571 487 Size: 683 56 1229 1086 30 3250 Fragments arranged by size: 3250 1229 1086 683 56 30 BtsI GCAGTG_nn' Cuts at: 2612 3850 4644 4664 2612 Size: 1238 794 20 4282 Fragments arranged by size: 4282 1238 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6334 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6299 DraI TTT'AAA Cuts at: 3638 3638 Size: 6334 DraIII CAC_nnn'GTG Cuts at: 2584 2584 Size: 6334 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 2735 5341 809 Size: 507 1419 2606 1802 Fragments arranged by size: 2606 1802 1419 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2106 2254 2271 Size: 54 1425 50 275 18 276 148 17 Cuts at: 2271 2643 2876 2943 3003 3781 4670 6299 8 Size: 372 233 67 60 778 889 1629 43 Fragments arranged by size: 1629 1425 889 778 372 276 275 233 148 67 60 54 50 43 18 17 EagI C'GGCC_G Cuts at: 1537 1812 2106 2254 2876 3781 1537 Size: 275 294 148 622 905 4090 Fragments arranged by size: 4090 905 622 294 275 148 EarI CTCTTCn'nnn_ Cuts at: 1353 2204 2543 4264 1353 Size: 851 339 1721 3423 Fragments arranged by size: 3423 1721 851 339 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2987 3031 3075 3266 3449 4921 Size: 1064 138 1398 44 44 191 183 1472 Cuts at: 4921 5295 5441 387 Size: 374 146 1280 Fragments arranged by size: 1472 1398 1280 1064 374 191 183 146 138 44 44 EcoICRI GAG'CTC Cuts at: 2062 2062 Size: 6334 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2264 4397 4994 5781 6049 1239 Size: 393 632 2133 597 787 268 1524 Fragments arranged by size: 2133 1524 787 632 597 393 268 EcoNI CCTnn'n_nnAGG Cuts at: 2075 3245 3428 3536 2075 Size: 1170 183 108 4873 Fragments arranged by size: 4873 1170 183 108 EcoO109I rG'GnC_Cy Cuts at: 2152 2417 3077 3361 3523 3524 3550 2152 Size: 265 660 284 162 1 26 4936 Fragments arranged by size: 4936 660 284 265 162 26 1 EcoRI G'AATT_C Cuts at: 3643 4021 3643 Size: 378 5956 FalI AAGnnnnnCTTnnnnnnnn_nnnnn' Cuts at: 2575 2575 Size: 6334 FspI TGC'GCA Cuts at: 4840 4840 Size: 6334 HaeII r_GCGC'y Cuts at: 1678 2251 2319 2614 2759 2829 2853 3284 5481 Size: 573 68 295 145 70 24 431 2197 Cuts at: 5481 1678 Size: 2531 Fragments arranged by size: 2531 2197 573 431 295 145 70 68 24 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 4975 5007 5049 5081 1373 Size: 32 135 32 3403 32 42 32 2626 Fragments arranged by size: 3403 2626 135 42 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2524 3678 3924 669 Size: 988 281 586 1154 246 3079 Fragments arranged by size: 3079 1154 988 586 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 6334 HpaI GTT'AAC Cuts at: 3924 3924 Size: 6334 KasI G'GCGC_C Cuts at: 2610 2849 3280 2610 Size: 239 431 5664 Fragments arranged by size: 5664 431 239 KpnI G_GTAC'C Cuts at: 3659 3659 Size: 6334 MfeI C'AATT_G Cuts at: 3933 3933 Size: 6334 MluI A'CGCG_T Cuts at: 6210 6210 Size: 6334 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2793 5448 5632 1305 Size: 1488 2655 184 2007 Fragments arranged by size: 2655 2007 1488 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2273 2645 2945 10 Size: 54 1425 343 441 372 300 3399 Fragments arranged by size: 3399 1425 441 372 343 300 54 MslI CAynn'nnrTG Cuts at: 518 1166 2009 2954 4292 4651 4810 518 Size: 648 843 945 1338 359 159 2042 Fragments arranged by size: 2042 1338 945 843 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2253 2423 2746 2941 3028 3475 3750 4427 Size: 647 170 323 195 87 447 275 677 Cuts at: 4427 5575 5820 1606 Size: 1148 245 2120 Fragments arranged by size: 2120 1148 677 647 447 323 275 245 195 170 87 NaeI GCC'GGC Cuts at: 2603 2603 Size: 6334 NarI GG'CG_CC Cuts at: 2611 2850 3281 2611 Size: 239 431 5664 Fragments arranged by size: 5664 431 239 NcoI C'CATG_G Cuts at: 513 1065 513 Size: 552 5782 NdeI CA'TA_TG Cuts at: 387 387 Size: 6334 NgoMIV G'CCGG_C Cuts at: 2601 2601 Size: 6334 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6334 NotI GC'GGCC_GC Cuts at: 2254 2876 3781 2254 Size: 622 905 4807 Fragments arranged by size: 4807 905 622 NspI r_CATG'y Cuts at: 1132 1229 3692 5237 1132 Size: 97 2463 1545 2229 Fragments arranged by size: 2463 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6334 PciI A'CATG_T Cuts at: 5233 5233 Size: 6334 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6334 PfoI T'CCnGG_A Cuts at: 3218 3218 Size: 6334 PmeI GTTT'AAAC Cuts at: 3638 3638 Size: 6334 PmlI CAC'GTG Cuts at: 2296 2296 Size: 6334 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4403 4435 5916 5948 4403 Size: 32 1481 32 4789 Fragments arranged by size: 4789 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 3550 3550 Size: 6334 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 6334 PsiI TTA'TAA Cuts at: 3904 3904 Size: 6334 PspOMI G'GGCC_C Cuts at: 2152 2417 3076 3361 3523 2152 Size: 265 659 285 162 4963 Fragments arranged by size: 4963 659 285 265 162 PstI C_TGCA'G Cuts at: 830 2079 2463 3686 830 Size: 1249 384 1223 3478 Fragments arranged by size: 3478 1249 1223 384 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6334 PvuII CAG'CTG Cuts at: 2746 2746 Size: 6334 SacI G_AGCT'C Cuts at: 2064 2064 Size: 6334 SacII CC_GC'GG Cuts at: 2254 2424 2254 Size: 170 6164 SalI G'TCGA_C Cuts at: 1936 3676 1936 Size: 1740 4594 SapI GCTCTTCn'nnn_ Cuts at: 2204 2204 Size: 6334 SbfI CC_TGCA'GG Cuts at: 3686 3686 Size: 6334 ScaI AGT'ACT Cuts at: 3622 4582 6294 3622 Size: 960 1712 3662 Fragments arranged by size: 3662 1712 960 SfcI C'TryA_G Cuts at: 826 1045 2075 2459 3682 4817 5498 5689 826 Size: 219 1030 384 1223 1135 681 191 1471 Fragments arranged by size: 1471 1223 1135 1030 681 384 219 191 SfoI GGC'GCC Cuts at: 2612 2851 3282 2612 Size: 239 431 5664 Fragments arranged by size: 5664 431 239 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6334 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6334 SmaI CCC'GGG Cuts at: 1872 2157 3075 3341 3506 3661 1872 Size: 285 918 266 165 155 4545 Fragments arranged by size: 4545 918 285 266 165 155 SmlI C'TyrA_G Cuts at: 820 1017 1943 4438 5339 5601 820 Size: 197 926 2495 901 262 1553 Fragments arranged by size: 2495 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 4125 493 Size: 3632 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6334 SphI G_CATG'C Cuts at: 1132 3692 1132 Size: 2560 3774 SrfI GCCC'GGGC Cuts at: 2157 3341 2157 Size: 1184 5150 SspI AAT'ATT Cuts at: 5 52 4258 5 Size: 47 4206 2081 Fragments arranged by size: 4206 2081 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6334 StyI C'CwwG_G Cuts at: 513 1065 2148 3183 3773 513 Size: 552 1083 1035 590 3074 Fragments arranged by size: 3074 1083 1035 590 552 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4361 4378 4531 4716 5161 325 Size: 1255 2781 17 153 185 445 1498 Fragments arranged by size: 2781 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 3620 4580 6292 Size: 274 80 33 51 1443 1643 960 1712 Cuts at: 6292 96 Size: 138 Fragments arranged by size: 1712 1643 1443 960 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3564 3596 6294 6326 3564 Size: 32 2698 32 3572 Fragments arranged by size: 3572 2698 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6334 XbaI T'CTAG_A Cuts at: 3670 3670 Size: 6334 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2281 2953 2281 Size: 672 5662 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6334 XmaI C'CCGG_G Cuts at: 1870 2155 3073 3339 3504 3659 1870 Size: 285 918 266 165 155 4545 Fragments arranged by size: 4545 918 285 266 165 155 XmnI GAAnn'nnTTC Cuts at: 2228 2369 4463 2228 Size: 141 2094 4099 Fragments arranged by size: 4099 2094 141 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 4142 276 Size: 53 83 186 936 2608 2468 Fragments arranged by size: 2608 2468 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbeI BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FalI FspI HaeII Hin4I HincII HindIII HpaI KasI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NarI NcoI NdeI NgoMIV NheI NotI NspI PasI PciI PflMI PfoI PmeI PmlI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SacI SacII SalI SapI SbfI ScaI SfcI SfoI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SrfI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbvCI BfrBI BplI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoRV FseI FspAI NruI NsiI PacI PspXI PsrI RsrII SanDI SexAI SfiI SwaI