Restriction map for
pFN21AB8716
(Linear) (Six-base) MAPSORT of: KIBB8716.seq Check: 9358 from: 1 to: 1629
KIBB8716.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 379 394 1629
Size: 379 15 1235
Fragments arranged by size:
1235 379 15
AlwNI CAG_nnn'CTG
Cuts at: 0 222 744 1629
Size: 222 522 885
Fragments arranged by size:
885 522 222
ApaI G_GGCC'C
Cuts at: 0 151 416 1075 1360 1522 1629
Size: 151 265 659 285 162 107
Fragments arranged by size:
659 285 265 162 151 107
ApaLI G'TGCA_C
Cuts at: 0 333 1147 1629
Size: 333 814 482
Fragments arranged by size:
814 482 333
AvaI C'yCGr_G
Cuts at: 0 150 795 1068 1300 1334 1350 1499 1629
Size: 150 645 273 232 34 16 149 130
Fragments arranged by size:
645 273 232 150 149 130 34 16
BamHI G'GATC_C
Cuts at: 0 893 1629
Size: 893 736
BanI G'GyrC_C
Cuts at: 0 605 844 1165 1191 1275 1629
Size: 605 239 321 26 84 354
Fragments arranged by size:
605 354 321 239 84 26
BanII G_rGCy'C
Cuts at: 0 59 151 211 416 839 1075 1360 1436
Size: 59 92 60 205 423 236 285 76
Cuts at: 1436 1522 1629
Size: 86 107
Fragments arranged by size:
423 285 236 205 107 92 86 76
60 59
BbeI G_GCGC'C
Cuts at: 0 609 848 1279 1629
Size: 609 239 431 350
Fragments arranged by size:
609 431 350 239
BbsI GAAGACnn'nnnn_
Cuts at: 0 1000 1629
Size: 1000 629
BlpI GC'TnA_GC
Cuts at: 0 307 1629
Size: 307 1322
Bme1580I G_kGCm'C
Cuts at: 0 11 151 320 337 416 1075 1151 1196
Size: 11 140 169 17 79 659 76 45
Cuts at: 1196 1360 1522 1629
Size: 164 162 107
Fragments arranged by size:
659 169 164 162 140 107 79 76
45 17 11
BmgBI CAC'GTC
Cuts at: 0 1146 1368 1539 1629
Size: 1146 222 171 90
Fragments arranged by size:
1146 222 171 90
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1629
Size: 259 1370
Bpu10I CC'TnA_GC
Cuts at: 0 1447 1629
Size: 1447 182
BsaAI yAC'GTr
Cuts at: 0 6 291 1629
Size: 6 285 1338
Fragments arranged by size:
1338 285 6
BsaBI GATnn'nnATC
Cuts at: 0 1579 1629
Size: 1579 50
BsaHI Gr'CG_yC
Cuts at: 0 606 773 845 1276 1629
Size: 606 167 72 431 353
Fragments arranged by size:
606 431 353 167 72
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 1519 1522 1629
Size: 1519 3 107
Fragments arranged by size:
1519 107 3
BseYI C'CCAG_C
Cuts at: 0 15 20 378 817 1260 1629
Size: 15 5 358 439 443 369
Fragments arranged by size:
443 439 369 358 15 5
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 556 823 1459 1629
Size: 556 267 636 170
Fragments arranged by size:
636 556 267 170
BsiEI CG_ry'CG
Cuts at: 0 104 252 874 1610 1629
Size: 104 148 622 736 19
Fragments arranged by size:
736 622 148 104 19
BsiHKAI G_wGCw'C
Cuts at: 0 59 337 1151 1629
Size: 59 278 814 478
Fragments arranged by size:
814 478 278 59
Bsp1286I G_dGCh'C
Cuts at: 0 11 59 151 211 320 337 416 839
Size: 11 48 92 60 109 17 79 423
Cuts at: 839 1075 1151 1196 1360 1436 1522 1629
Size: 236 76 45 164 76 86 107
Fragments arranged by size:
423 236 164 109 107 92 86 79
76 76 60 48 45 17 11
BspHI T'CATG_A
Cuts at: 0 497 1629
Size: 497 1132
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 379 394 1629
Size: 379 15 1235
Fragments arranged by size:
1235 379 15
BsrBI CCG'CTC
Cuts at: 0 611 1034 1296 1373 1629
Size: 611 423 262 77 256
Fragments arranged by size:
611 423 262 256 77
BsrFI r'CCGG_y
Cuts at: 0 596 683 1629
Size: 596 87 946
Fragments arranged by size:
946 596 87
BssHII G'CGCG_C
Cuts at: 0 113 374 985 1126 1629
Size: 113 261 611 141 503
Fragments arranged by size:
611 503 261 141 113
BstYI r'GATC_y
Cuts at: 0 134 893 1574 1629
Size: 134 759 681 55
Fragments arranged by size:
759 681 134 55
BtgI C'CryG_G
Cuts at: 0 246 416 782 809 860 1001 1629
Size: 246 170 366 27 51 141 628
Fragments arranged by size:
628 366 246 170 141 51 27
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 450 1536 1566 1629
Size: 450 1086 30 63
Fragments arranged by size:
1086 450 63 30
BtsI GCAGTG_nn'
Cuts at: 0 607 1629
Size: 607 1022
DraIII CAC_nnn'GTG
Cuts at: 0 579 1629
Size: 579 1050
DrdI GACnn_nn'nnGTC
Cuts at: 0 730 1629
Size: 730 899
EaeI y'GGCC_r
Cuts at: 0 101 249 266 638 871 938 998 1629
Size: 101 148 17 372 233 67 60 631
Fragments arranged by size:
631 372 233 148 101 67 60 17
EagI C'GGCC_G
Cuts at: 0 101 249 871 1629
Size: 101 148 622 758
Fragments arranged by size:
758 622 148 101
EarI CTCTTCn'nnn_
Cuts at: 0 199 538 1629
Size: 199 339 1091
Fragments arranged by size:
1091 339 199
EciI GGCGGAnnnnnnnnn_nn'
Cuts at: 0 982 1026 1070 1261 1444 1629
Size: 982 44 44 191 183 185
Fragments arranged by size:
982 191 185 183 44 44
EcoICRI GAG'CTC
Cuts at: 0 57 1629
Size: 57 1572
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 259 1629
Size: 259 1370
EcoNI CCTnn'n_nnAGG
Cuts at: 0 70 1240 1423 1531 1629
Size: 70 1170 183 108 98
Fragments arranged by size:
1170 183 108 98 70
EcoO109I rG'GnC_Cy
Cuts at: 0 147 412 1072 1356 1518 1519 1545 1629
Size: 147 265 660 284 162 1 26 84
Fragments arranged by size:
660 284 265 162 147 84 26 1
FalI AAGnnnnnCTTnnnnnnnn_nnnnn'
Cuts at: 0 570 1629
Size: 570 1059
HaeII r_GCGC'y
Cuts at: 0 246 314 609 754 824 848 1279 1629
Size: 246 68 295 145 70 24 431 350
Fragments arranged by size:
431 350 295 246 145 70 68 24
HincII GTy'rAC
Cuts at: 0 519 1629
Size: 519 1110
KasI G'GCGC_C
Cuts at: 0 605 844 1275 1629
Size: 605 239 431 354
Fragments arranged by size:
605 431 354 239
MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn'
Cuts at: 0 788 1629
Size: 788 841
MscI TGG'CCA
Cuts at: 0 268 640 940 1629
Size: 268 372 300 689
Fragments arranged by size:
689 372 300 268
MslI CAynn'nnrTG
Cuts at: 0 949 1629
Size: 949 680
MspA1I CmG'CkG
Cuts at: 0 248 418 741 936 1023 1470 1629
Size: 248 170 323 195 87 447 159
Fragments arranged by size:
447 323 248 195 170 159 87
NaeI GCC'GGC
Cuts at: 0 598 1629
Size: 598 1031
NarI GG'CG_CC
Cuts at: 0 606 845 1276 1629
Size: 606 239 431 353
Fragments arranged by size:
606 431 353 239
NgoMIV G'CCGG_C
Cuts at: 0 596 1629
Size: 596 1033
NotI GC'GGCC_GC
Cuts at: 0 249 871 1629
Size: 249 622 758
Fragments arranged by size:
758 622 249
PfoI T'CCnGG_A
Cuts at: 0 1213 1629
Size: 1213 416
PmlI CAC'GTG
Cuts at: 0 291 1629
Size: 291 1338
PpuMI rG'GwC_Cy
Cuts at: 0 1545 1629
Size: 1545 84
PspOMI G'GGCC_C
Cuts at: 0 147 412 1071 1356 1518 1629
Size: 147 265 659 285 162 111
Fragments arranged by size:
659 285 265 162 147 111
PstI C_TGCA'G
Cuts at: 0 74 458 1629
Size: 74 384 1171
Fragments arranged by size:
1171 384 74
PvuII CAG'CTG
Cuts at: 0 741 1629
Size: 741 888
SacI G_AGCT'C
Cuts at: 0 59 1629
Size: 59 1570
SacII CC_GC'GG
Cuts at: 0 249 419 1629
Size: 249 170 1210
Fragments arranged by size:
1210 249 170
SapI GCTCTTCn'nnn_
Cuts at: 0 199 1629
Size: 199 1430
ScaI AGT'ACT
Cuts at: 0 1617 1629
Size: 1617 12
SfcI C'TryA_G
Cuts at: 0 70 454 1629
Size: 70 384 1175
Fragments arranged by size:
1175 384 70
SfoI GGC'GCC
Cuts at: 0 607 846 1277 1629
Size: 607 239 431 352
Fragments arranged by size:
607 431 352 239
SmaI CCC'GGG
Cuts at: 0 152 1070 1336 1501 1629
Size: 152 918 266 165 128
Fragments arranged by size:
918 266 165 152 128
SrfI GCCC'GGGC
Cuts at: 0 152 1336 1629
Size: 152 1184 293
Fragments arranged by size:
1184 293 152
StyI C'CwwG_G
Cuts at: 0 143 1178 1629
Size: 143 1035 451
Fragments arranged by size:
1035 451 143
TatI w'GTAC_w
Cuts at: 0 1615 1629
Size: 1615 14
TstI CACnnnnnnTCCnnnnnnn_nnnnn'
Cuts at: 0 1559 1591 1629
Size: 1559 32 38
Fragments arranged by size:
1559 38 32
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 276 948 1629
Size: 276 672 681
Fragments arranged by size:
681 672 276
XmaI C'CCGG_G
Cuts at: 0 150 1068 1334 1499 1629
Size: 150 918 266 165 130
Fragments arranged by size:
918 266 165 150 130
XmnI GAAnn'nnTTC
Cuts at: 0 223 364 1629
Size: 223 141 1265
Fragments arranged by size:
1265 223 141
Enzymes that do cut:
AarI AlwNI ApaI ApaLI AvaI BamHI BanI BanII
BbeI BbsI BlpI Bme1580I BmgBI BpmI Bpu10I BsaAI
BsaBI BsaHI BseRI BseYI BsgI BsiEI BsiHKAI Bsp1286I
BspHI BspMI BsrBI BsrFI BssHII BstYI BtgI BtgZI
BtsI DraIII DrdI EaeI EagI EarI EciI EcoICRI
Eco57MI EcoNI EcoO109I FalI HaeII HincII KasI MmeI
MscI MslI MspA1I NaeI NarI NgoMIV NotI PfoI
PmlI PpuMI PspOMI PstI PvuII SacI SacII SapI
ScaI SfcI SfoI SmaI SrfI StyI TatI TstI
XcmI XmaI XmnI
Enzymes that do not cut:
AatII AccI Acc65I AclI AcuI AfeI AflII AflIII
AgeI AhdI AleI AlfI AloI ApoI AscI AseI
AsiSI AvrII BaeI BbvCI BcgI BciVI BclI BfrBI
BglI BglII BmrI BmtI BplI BpuEI BsaI BsaWI
BsaXI BsiWI BsmI BsmBI BspEI BsrDI BsrGI BssSI
BstAPI BstBI BstEII BstXI BstZ17I Bsu36I ClaI CspCI
DraI EcoRI EcoRV FseI FspI FspAI Hin4I HindIII
HpaI KpnI MfeI MluI NcoI NdeI NheI NruI
NsiI NspI PacI PasI PciI PflMI PmeI PpiI
PshAI PsiI PspXI PsrI PvuI RsrII SalI SanDI
SbfI SexAI SfiI SgfI SgrAI SmlI SnaBI SpeI
SphI SspI StuI SwaI TaqII Tth111I XbaI XhoI
ZraI