(Circular) (Six-base) MAPSORT of: pFN21AB8715.seq Check: 9956 from: 1 to: 5863 pFN21AB8715 5863 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2140 2575 2140 Size: 435 5428 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3673 278 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3206 1937 Size: 1269 4594 Acc65I G'GTAC_C Cuts at: 3184 3184 Size: 5863 AclI AA'CG_TT Cuts at: 1563 3990 4363 1563 Size: 2427 373 3063 Fragments arranged by size: 3063 2427 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3926 5310 5578 1632 Size: 2294 1384 268 1917 Fragments arranged by size: 2294 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5666 AflIII A'CryG_T Cuts at: 1203 4762 5739 1203 Size: 3559 977 1327 Fragments arranged by size: 3559 1327 977 AgeI A'CCGG_T Cuts at: 4694 4694 Size: 5863 AhdI GACnn_n'nnGTC Cuts at: 2743 4592 2743 Size: 1849 4014 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5863 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5863 AlwNI CAG_nnn'CTG Cuts at: 2590 2893 2998 3606 5178 2590 Size: 303 105 608 1572 3275 Fragments arranged by size: 3275 1572 608 303 105 ApaI G_GGCC'C Cuts at: 2021 2021 Size: 5863 ApaLI G'TGCA_C Cuts at: 2153 3920 5076 2153 Size: 1767 1156 2940 Fragments arranged by size: 2940 1767 1156 ApoI r'AATT_y Cuts at: 1493 3172 3398 3550 5659 1493 Size: 1679 226 152 2109 1697 Fragments arranged by size: 2109 1697 1679 226 152 AseI AT'TA_AT Cuts at: 160 1032 4417 160 Size: 872 3385 1606 Fragments arranged by size: 3385 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 AvaI C'yCGr_G Cuts at: 1870 1943 2653 3188 1870 Size: 73 710 535 4545 Fragments arranged by size: 4545 710 535 73 BamHI G'GATC_C Cuts at: 3193 3560 3193 Size: 367 5496 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3065 3184 4639 618 Size: 325 217 627 1278 119 1455 1842 Fragments arranged by size: 1842 1455 1278 627 325 217 119 BanII G_rGCy'C Cuts at: 1391 2021 2813 3182 1391 Size: 630 792 369 4072 Fragments arranged by size: 4072 792 630 369 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2763 928 Size: 971 864 4028 Fragments arranged by size: 4028 971 864 BbvCI CC'TCA_GC Cuts at: 2281 2281 Size: 5863 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4054 4088 1903 Size: 34 50 34 2033 34 3678 Fragments arranged by size: 3678 2033 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2357 3755 4965 2357 Size: 1398 1210 3255 Fragments arranged by size: 3255 1398 1210 BclI T'GATC_A Cuts at: 1548 1548 Size: 5863 BfrBI ATG'CAT Cuts at: 2008 2008 Size: 5863 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2025 2256 2390 Size: 107 122 71 631 605 353 231 134 Cuts at: 2390 2440 2531 2638 4474 136 Size: 50 91 107 1836 1525 Fragments arranged by size: 1836 1525 631 605 353 231 134 122 107 107 91 71 50 BglII A'GATC_T Cuts at: 5858 5858 Size: 5863 BlpI GC'TnA_GC Cuts at: 2480 3280 2480 Size: 800 5063 Bme1580I G_kGCm'C Cuts at: 1790 2021 2157 2261 2431 3101 3924 5080 1790 Size: 231 136 104 170 670 823 1156 2573 Fragments arranged by size: 2573 1156 823 670 231 170 136 104 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5863 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2761 2842 3594 Size: 416 53 83 399 24 1344 81 752 Cuts at: 3594 4552 442 Size: 958 1753 Fragments arranged by size: 1753 1344 958 752 416 399 83 81 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5863 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2830 2830 Size: 5863 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 3020 4523 1239 Size: 1781 1503 2579 Fragments arranged by size: 2579 1781 1503 Bpu10I CC'TnA_GC Cuts at: 2207 2281 2207 Size: 74 5789 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3988 4853 5151 3988 Size: 865 298 4700 Fragments arranged by size: 4700 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 3098 4526 882 Size: 617 1599 1428 2219 Fragments arranged by size: 2219 1599 1428 617 BsaAI yAC'GTr Cuts at: 493 1204 3654 493 Size: 711 2450 2702 Fragments arranged by size: 2702 2450 711 BsaBI GATnn'nnATC Cuts at: 5857 5857 Size: 5863 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 2601 3670 4052 4687 Size: 53 83 186 936 1068 1069 382 635 Cuts at: 4687 275 Size: 1451 Fragments arranged by size: 1451 1069 1068 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 2553 4296 4694 4968 5115 5526 2553 Size: 1743 398 274 147 411 2890 Fragments arranged by size: 2890 1743 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 3078 3108 1178 Size: 30 165 30 1675 30 3933 Fragments arranged by size: 3933 1675 165 30 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2047 2803 2968 1186 Size: 861 756 165 4081 Fragments arranged by size: 4081 861 756 165 BseYI C'CCAG_C Cuts at: 2038 2393 2543 2837 5066 5562 2038 Size: 355 150 294 2229 496 2339 Fragments arranged by size: 2339 2229 496 355 294 150 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2367 2629 2813 1163 Size: 573 631 262 184 4213 Fragments arranged by size: 4213 631 573 262 184 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3313 4074 5102 1540 Size: 275 186 1312 761 1028 2301 Fragments arranged by size: 2301 1312 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2084 2157 3924 4009 5080 2084 Size: 73 1767 85 1071 2867 Fragments arranged by size: 2867 1767 1071 85 73 BsmI GAATG_Cn' Cuts at: 3374 3467 3374 Size: 93 5770 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2021 2084 2157 2261 2431 2813 3101 Size: 399 231 63 73 104 170 382 288 Cuts at: 3101 3182 3924 4009 5080 1391 Size: 81 742 85 1071 2174 Fragments arranged by size: 2174 1071 742 399 382 288 231 170 104 85 81 73 63 BspEI T'CCGG_A Cuts at: 5526 5526 Size: 5863 BspHI T'CATG_A Cuts at: 3752 5482 3752 Size: 1730 4133 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2140 2575 3218 844 Size: 1296 435 643 3489 Fragments arranged by size: 3489 1296 643 435 BsrBI CCG'CTC Cuts at: 1394 1823 3750 1394 Size: 429 1927 3507 Fragments arranged by size: 3507 1927 429 BsrDI GCAATG_nn' Cuts at: 65 1248 4358 4532 65 Size: 1183 3110 174 1396 Fragments arranged by size: 3110 1396 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 2102 2157 2351 2525 4507 4694 1700 Size: 110 292 55 194 174 1982 187 2869 Fragments arranged by size: 2869 1982 292 194 187 174 110 55 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5863 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5863 BssSI C'ACGA_G Cuts at: 3923 4935 3923 Size: 1012 4851 BstAPI GCAn_nnn'nTGC Cuts at: 794 2161 2590 2893 794 Size: 1367 429 303 3764 Fragments arranged by size: 3764 1367 429 303 BstBI TT'CG_AA Cuts at: 4667 4667 Size: 5863 BstEII G'GTnAC_C Cuts at: 1184 3112 1184 Size: 1928 3935 BstYI r'GATC_y Cuts at: 1972 3193 3560 3945 3962 5414 5500 5512 5858 Size: 1221 367 385 17 1452 86 12 346 Cuts at: 5858 1972 Size: 1977 Fragments arranged by size: 1977 1452 1221 385 367 346 86 17 12 Bsu36I CC'TnA_GG Cuts at: 2636 2783 2636 Size: 147 5716 BtgI C'CryG_G Cuts at: 513 1065 2023 2422 2438 2450 2954 3118 513 Size: 552 958 399 16 12 504 164 3258 Fragments arranged by size: 3258 958 552 504 399 164 16 12 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5124 Fragments arranged by size: 5124 683 56 BtsI GCAGTG_nn' Cuts at: 2042 2322 2429 2474 3379 4173 4193 2042 Size: 280 107 45 905 794 20 3712 Fragments arranged by size: 3712 905 794 280 107 45 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5863 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5828 DraI TTT'AAA Cuts at: 3167 3167 Size: 5863 DraIII CAC_nnn'GTG Cuts at: 2088 2088 Size: 5863 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4870 809 Size: 507 3554 1802 Fragments arranged by size: 3554 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2026 2382 3310 Size: 54 1425 50 275 18 196 356 928 Cuts at: 3310 4199 5828 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 928 889 356 275 196 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3310 1537 Size: 275 1498 4090 Fragments arranged by size: 4090 1498 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3793 1353 Size: 2440 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 2681 4450 4824 4970 387 Size: 1064 138 1092 1769 374 146 1280 Fragments arranged by size: 1769 1280 1092 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 3020 3926 4523 5310 5578 1239 Size: 393 1388 906 597 787 268 1524 Fragments arranged by size: 1524 1388 906 787 597 393 268 EcoO109I rG'GnC_Cy Cuts at: 2017 2018 2547 2613 3149 2017 Size: 1 529 66 536 4731 Fragments arranged by size: 4731 536 529 66 1 EcoRI G'AATT_C Cuts at: 3172 3550 3172 Size: 378 5485 FseI GG_CCGG'CC Cuts at: 2106 2106 Size: 5863 FspI TGC'GCA Cuts at: 4369 4369 Size: 5863 HaeII r_GCGC'y Cuts at: 1678 5010 1678 Size: 3332 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2729 2761 4504 4536 4578 Size: 32 135 32 1157 32 1743 32 42 Cuts at: 4578 4610 1373 Size: 32 2626 Fragments arranged by size: 2626 1743 1157 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3207 3453 669 Size: 988 281 1269 246 3079 Fragments arranged by size: 3079 1269 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5863 HpaI GTT'AAC Cuts at: 3453 3453 Size: 5863 KpnI G_GTAC'C Cuts at: 3188 3188 Size: 5863 MfeI C'AATT_G Cuts at: 3462 3462 Size: 5863 MluI A'CGCG_T Cuts at: 5739 5739 Size: 5863 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 3064 4977 5161 1305 Size: 1759 1913 184 2007 Fragments arranged by size: 2007 1913 1759 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2028 2384 10 Size: 54 1425 343 196 356 3489 Fragments arranged by size: 3489 1425 356 343 196 54 MslI CAynn'nnrTG Cuts at: 518 1166 3821 4180 4339 518 Size: 648 2655 359 159 2042 Fragments arranged by size: 2655 2042 648 359 159 MspA1I CmG'CkG Cuts at: 1606 2358 2543 2998 3279 3956 5104 5349 1606 Size: 752 185 455 281 677 1148 245 2120 Fragments arranged by size: 2120 1148 752 677 455 281 245 185 NaeI GCC'GGC Cuts at: 2104 2353 2527 2104 Size: 249 174 5440 Fragments arranged by size: 5440 249 174 NcoI C'CATG_G Cuts at: 513 1065 2023 2954 3118 513 Size: 552 958 931 164 3258 Fragments arranged by size: 3258 958 931 552 164 NdeI CA'TA_TG Cuts at: 387 387 Size: 5863 NgoMIV G'CCGG_C Cuts at: 2102 2351 2525 2102 Size: 249 174 5440 Fragments arranged by size: 5440 249 174 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5863 NotI GC'GGCC_GC Cuts at: 3310 3310 Size: 5863 NsiI A_TGCA'T Cuts at: 2010 2010 Size: 5863 NspI r_CATG'y Cuts at: 1132 1229 2012 2279 2371 3221 4766 1132 Size: 97 783 267 92 850 1545 2229 Fragments arranged by size: 2229 1545 850 783 267 97 92 PasI CC'CwG_GG Cuts at: 1348 2176 2855 1348 Size: 828 679 4356 Fragments arranged by size: 4356 828 679 PciI A'CATG_T Cuts at: 4762 4762 Size: 5863 PflMI CCAn_nnn'nTGG Cuts at: 1108 2176 1108 Size: 1068 4795 PmeI GTTT'AAAC Cuts at: 3167 3167 Size: 5863 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3932 3964 5445 5477 3932 Size: 32 1481 32 4318 Fragments arranged by size: 4318 1481 32 32 PpuMI rG'GwC_Cy Cuts at: 2613 3149 2613 Size: 536 5327 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5863 PsiI TTA'TAA Cuts at: 3433 3433 Size: 5863 PspOMI G'GGCC_C Cuts at: 2017 2017 Size: 5863 PstI C_TGCA'G Cuts at: 830 2505 2586 2595 2898 2994 3215 830 Size: 1675 81 9 303 96 221 3478 Fragments arranged by size: 3478 1675 303 221 96 81 9 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5863 PvuII CAG'CTG Cuts at: 2358 2543 2998 2358 Size: 185 455 5223 Fragments arranged by size: 5223 455 185 SalI G'TCGA_C Cuts at: 1936 3205 1936 Size: 1269 4594 SbfI CC_TGCA'GG Cuts at: 3215 3215 Size: 5863 ScaI AGT'ACT Cuts at: 4111 5823 4111 Size: 1712 4151 SfcI C'TryA_G Cuts at: 826 1045 2501 2582 2591 2894 2990 3211 4346 Size: 219 1456 81 9 303 96 221 1135 Cuts at: 4346 5027 5218 826 Size: 681 191 1471 Fragments arranged by size: 1471 1456 1135 681 303 221 219 191 96 81 9 SfiI GGCCn_nnn'nGGCC Cuts at: 2025 2025 Size: 5863 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5863 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5863 SmaI CCC'GGG Cuts at: 1872 2655 3190 1872 Size: 783 535 4545 Fragments arranged by size: 4545 783 535 SmlI C'TyrA_G Cuts at: 820 1017 1943 3967 4868 5130 820 Size: 197 926 2024 901 262 1553 Fragments arranged by size: 2024 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3654 493 Size: 3161 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5863 SphI G_CATG'C Cuts at: 1132 2012 2279 3221 1132 Size: 880 267 942 3774 Fragments arranged by size: 3774 942 880 267 SspI AAT'ATT Cuts at: 5 52 3787 5 Size: 47 3735 2081 Fragments arranged by size: 3735 2081 47 StuI AGG'CCT Cuts at: 1517 2199 2331 2701 2788 2826 2912 1517 Size: 682 132 370 87 38 86 4468 Fragments arranged by size: 4468 682 370 132 87 86 38 StyI C'CwwG_G Cuts at: 513 1065 2023 2332 2616 2877 2954 3118 3302 Size: 552 958 309 284 261 77 164 184 Cuts at: 3302 513 Size: 3074 Fragments arranged by size: 3074 958 552 309 284 261 184 164 77 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2696 3890 3907 4060 4245 4690 325 Size: 1255 1116 1194 17 153 185 445 1498 Fragments arranged by size: 1498 1255 1194 1116 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4109 5821 96 Size: 274 80 33 51 1443 2132 1712 138 Fragments arranged by size: 2132 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3078 3110 5823 5855 3078 Size: 32 2713 32 3086 Fragments arranged by size: 3086 2713 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5863 XbaI T'CTAG_A Cuts at: 3199 3199 Size: 5863 XcmI CCAnnnn_n'nnnnTGG Cuts at: 3055 3125 3055 Size: 70 5793 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5863 XmaI C'CCGG_G Cuts at: 1870 2653 3188 1870 Size: 783 535 4545 Fragments arranged by size: 4545 783 535 XmnI GAAnn'nnTTC Cuts at: 3992 3992 Size: 5863 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3671 276 Size: 53 83 186 936 2137 2468 Fragments arranged by size: 2468 2137 936 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BbvCI BcgI BciVI BclI BfrBI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI Bsu36I BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FseI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NaeI NcoI NdeI NgoMIV NheI NotI NsiI NspI PasI PciI PflMI PmeI PpiI PpuMI PshAI PsiI PspOMI PstI PvuI PvuII SalI SbfI ScaI SfcI SfiI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BsiWI BsmBI BstXI BstZ17I EcoICRI EcoNI EcoRV FalI FspAI KasI NarI NruI PacI PfoI PmlI PspXI PsrI RsrII SacI SacII SanDI SapI SexAI SfoI SrfI SwaI