(Circular) (Six-base) MAPSORT of: pFN21AB8709.seq Check: 3518 from: 1 to: 5920 pFN21AB8709 5920 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AarI CACCTGCnnnn'nnnn_ Cuts at: 2346 2346 Size: 5920 AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3119 3730 278 Size: 53 83 186 936 1583 611 2468 Fragments arranged by size: 2468 1583 936 611 186 83 53 AccI GT'mk_AC Cuts at: 1937 2553 3263 1937 Size: 616 710 4594 Fragments arranged by size: 4594 710 616 Acc65I G'GTAC_C Cuts at: 3241 3241 Size: 5920 AclI AA'CG_TT Cuts at: 1563 2133 4047 4420 1563 Size: 570 1914 373 3063 Fragments arranged by size: 3063 1914 570 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2109 2131 3044 3983 5367 5635 1632 Size: 477 22 913 939 1384 268 1917 Fragments arranged by size: 1917 1384 939 913 477 268 22 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5723 AflIII A'CryG_T Cuts at: 1203 4819 5796 1203 Size: 3616 977 1327 Fragments arranged by size: 3616 1327 977 AgeI A'CCGG_T Cuts at: 4751 4751 Size: 5920 AhdI GACnn_n'nnGTC Cuts at: 4649 4649 Size: 5920 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5920 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5920 AlwNI CAG_nnn'CTG Cuts at: 2281 2338 3663 5235 2281 Size: 57 1325 1572 2966 Fragments arranged by size: 2966 1572 1325 57 ApaI G_GGCC'C Cuts at: 2266 2266 Size: 5920 ApaLI G'TGCA_C Cuts at: 3977 5133 3977 Size: 1156 4764 ApoI r'AATT_y Cuts at: 1493 2479 2992 3229 3455 3607 5716 1493 Size: 986 513 237 226 152 2109 1697 Fragments arranged by size: 2109 1697 986 513 237 226 152 AseI AT'TA_AT Cuts at: 160 1032 4474 160 Size: 872 3442 1606 Fragments arranged by size: 3442 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 AvaI C'yCGr_G Cuts at: 1870 1943 3245 1870 Size: 73 1302 4545 Fragments arranged by size: 4545 1302 73 BamHI G'GATC_C Cuts at: 2074 3250 3617 2074 Size: 1176 367 4377 Fragments arranged by size: 4377 1176 367 BanI G'GyrC_C Cuts at: 618 943 1160 1787 2243 2695 3241 4696 618 Size: 325 217 627 456 452 546 1455 1842 Fragments arranged by size: 1842 1455 627 546 456 452 325 217 BanII G_rGCy'C Cuts at: 1391 2266 2748 3239 1391 Size: 875 482 491 4072 Fragments arranged by size: 4072 875 491 482 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2021 2785 928 Size: 971 122 764 4063 Fragments arranged by size: 4063 971 764 122 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 1987 2021 4111 4145 1903 Size: 34 50 34 2090 34 3678 Fragments arranged by size: 3678 2090 50 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3812 5022 3812 Size: 1210 4710 BclI T'GATC_A Cuts at: 1548 1548 Size: 5920 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4531 136 Size: 107 122 71 631 605 2859 1525 Fragments arranged by size: 2859 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 5915 5915 Size: 5920 BlpI GC'TnA_GC Cuts at: 3337 3337 Size: 5920 Bme1580I G_kGCm'C Cuts at: 1790 2246 2266 2700 3981 5137 1790 Size: 456 20 434 1281 1156 2573 Fragments arranged by size: 2573 1281 1156 456 434 20 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5920 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 2500 3105 3209 Size: 416 53 83 399 24 1083 605 104 Cuts at: 3209 3651 4609 442 Size: 442 958 1753 Fragments arranged by size: 1753 1083 958 605 442 416 399 104 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5920 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2449 2449 Size: 5920 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2258 2435 2444 2946 2972 3202 4580 1239 Size: 1019 177 9 502 26 230 1378 2579 Fragments arranged by size: 2579 1378 1019 502 230 177 26 9 Bpu10I CC'TnA_GC Cuts at: 2224 2224 Size: 5920 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 2194 4045 4910 5208 2194 Size: 1851 865 298 2906 Fragments arranged by size: 2906 1851 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4583 882 Size: 617 3084 2219 Fragments arranged by size: 3084 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 2776 3711 493 Size: 711 1572 935 2702 Fragments arranged by size: 2702 1572 935 711 BsaBI GATnn'nnATC Cuts at: 5914 5914 Size: 5920 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3015 3116 3727 4109 Size: 53 83 186 936 1482 101 611 382 Cuts at: 4109 4744 275 Size: 635 1451 Fragments arranged by size: 1482 1451 936 635 611 382 186 101 83 53 BsaWI w'CCGG_w Cuts at: 2806 4353 4751 5025 5172 5583 2806 Size: 1547 398 274 147 411 3143 Fragments arranged by size: 3143 1547 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5695 Fragments arranged by size: 5695 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2003 2672 3083 1186 Size: 817 669 411 4023 Fragments arranged by size: 4023 817 669 411 BseYI C'CCAG_C Cuts at: 2265 2578 3018 5123 5619 2265 Size: 313 440 2105 496 2566 Fragments arranged by size: 2566 2105 496 440 313 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2324 2783 2823 1163 Size: 573 588 459 40 4260 Fragments arranged by size: 4260 588 573 459 40 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3370 4131 5159 1540 Size: 275 186 1369 761 1028 2301 Fragments arranged by size: 2301 1369 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2720 2748 3981 4066 5137 2720 Size: 28 1233 85 1071 3503 Fragments arranged by size: 3503 1233 1071 85 28 BsmI GAATG_Cn' Cuts at: 3431 3524 3431 Size: 93 5827 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2246 2266 2700 2720 2748 3239 3981 Size: 399 456 20 434 20 28 491 742 Cuts at: 3981 4066 5137 1391 Size: 85 1071 2174 Fragments arranged by size: 2174 1071 742 491 456 434 399 85 28 20 20 BspEI T'CCGG_A Cuts at: 2806 5583 2806 Size: 2777 3143 BspHI T'CATG_A Cuts at: 2734 3159 3809 5539 2734 Size: 425 650 1730 3115 Fragments arranged by size: 3115 1730 650 425 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 2346 3275 844 Size: 1502 929 3489 Fragments arranged by size: 3489 1502 929 BsrBI CCG'CTC Cuts at: 1394 1823 3807 1394 Size: 429 1984 3507 Fragments arranged by size: 3507 1984 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2395 2788 2964 4415 4589 65 Size: 1183 1147 393 176 1451 174 1396 Fragments arranged by size: 1451 1396 1183 1147 393 176 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4564 4751 1700 Size: 110 2754 187 2869 Fragments arranged by size: 2869 2754 187 110 BsrGI T'GTAC_A Cuts at: 96 2019 2901 96 Size: 1923 882 3115 Fragments arranged by size: 3115 1923 882 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5920 BssSI C'ACGA_G Cuts at: 3980 4992 3980 Size: 1012 4908 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 5920 BstBI TT'CG_AA Cuts at: 4724 4724 Size: 5920 BstEII G'GTnAC_C Cuts at: 1184 2914 3100 1184 Size: 1730 186 4004 Fragments arranged by size: 4004 1730 186 BstYI r'GATC_y Cuts at: 1972 2074 2316 3250 3617 4002 4019 5471 5557 Size: 102 242 934 367 385 17 1452 86 Cuts at: 5557 5569 5915 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 934 385 367 346 242 102 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2070 2217 2382 513 Size: 552 1005 147 165 4051 Fragments arranged by size: 4051 1005 552 165 147 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 2818 487 Size: 683 56 1592 3589 Fragments arranged by size: 3589 1592 683 56 BtsI GCAGTG_nn' Cuts at: 3436 4230 4250 3436 Size: 794 20 5106 Fragments arranged by size: 5106 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5920 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5885 DraI TTT'AAA Cuts at: 3224 3224 Size: 5920 DraIII CAC_nnn'GTG Cuts at: 2167 2167 Size: 5920 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4927 809 Size: 507 3611 1802 Fragments arranged by size: 3611 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2251 3367 4256 Size: 54 1425 50 275 18 421 1116 889 Cuts at: 4256 5885 8 Size: 1629 43 Fragments arranged by size: 1629 1425 1116 889 421 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3367 1537 Size: 275 1555 4090 Fragments arranged by size: 4090 1555 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3850 1353 Size: 2497 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4507 4881 5027 387 Size: 1064 138 2918 374 146 1280 Fragments arranged by size: 2918 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 2746 2746 Size: 5920 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2109 2131 2258 2435 2444 2946 2972 Size: 393 477 22 127 177 9 502 26 Cuts at: 2972 3044 3202 3983 4580 5367 5635 1239 Size: 72 158 781 597 787 268 1524 Fragments arranged by size: 1524 787 781 597 502 477 393 268 177 158 127 72 26 22 9 EcoO109I rG'GnC_Cy Cuts at: 2116 2278 2116 Size: 162 5758 EcoRI G'AATT_C Cuts at: 3229 3607 3229 Size: 378 5542 FspI TGC'GCA Cuts at: 4426 4426 Size: 5920 HaeII r_GCGC'y Cuts at: 1678 5067 1678 Size: 3389 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2092 2124 4561 4593 4635 Size: 32 135 32 520 32 2437 32 42 Cuts at: 4635 4667 1373 Size: 32 2626 Fragments arranged by size: 2626 2437 520 135 42 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2137 2554 2599 3264 3510 669 Size: 988 281 199 417 45 665 246 3079 Fragments arranged by size: 3079 988 665 417 281 246 199 45 HindIII A'AGCT_T Cuts at: 748 748 Size: 5920 HpaI GTT'AAC Cuts at: 3510 3510 Size: 5920 KpnI G_GTAC'C Cuts at: 3245 3245 Size: 5920 MfeI C'AATT_G Cuts at: 3519 3519 Size: 5920 MluI A'CGCG_T Cuts at: 5796 5796 Size: 5920 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 5034 5218 1305 Size: 3729 184 2007 Fragments arranged by size: 3729 2007 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 2253 10 Size: 54 1425 343 421 3677 Fragments arranged by size: 3677 1425 421 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2216 2454 3158 3878 4237 4396 518 Size: 648 1050 238 704 720 359 159 2042 Fragments arranged by size: 2042 1050 720 704 648 359 238 159 MspA1I CmG'CkG Cuts at: 1606 2049 2758 3022 3086 3336 4013 5161 5406 Size: 443 709 264 64 250 677 1148 245 Cuts at: 5406 1606 Size: 2120 Fragments arranged by size: 2120 1148 709 677 443 264 250 245 64 NcoI C'CATG_G Cuts at: 513 1065 2070 2217 2382 513 Size: 552 1005 147 165 4051 Fragments arranged by size: 4051 1005 552 165 147 NdeI CA'TA_TG Cuts at: 387 387 Size: 5920 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5920 NotI GC'GGCC_GC Cuts at: 3367 3367 Size: 5920 NspI r_CATG'y Cuts at: 1132 1229 3278 4823 1132 Size: 97 2049 1545 2229 Fragments arranged by size: 2229 2049 1545 97 PasI CC'CwG_GG Cuts at: 1348 2239 1348 Size: 891 5029 PciI A'CATG_T Cuts at: 4819 4819 Size: 5920 PflMI CCAn_nnn'nTGG Cuts at: 1108 2071 2281 1108 Size: 963 210 4747 Fragments arranged by size: 4747 963 210 PfoI T'CCnGG_A Cuts at: 2484 2484 Size: 5920 PmeI GTTT'AAAC Cuts at: 3224 3224 Size: 5920 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3989 4021 5502 5534 3989 Size: 32 1481 32 4375 Fragments arranged by size: 4375 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5920 PsiI TTA'TAA Cuts at: 3490 3490 Size: 5920 PspOMI G'GGCC_C Cuts at: 2262 2262 Size: 5920 PstI C_TGCA'G Cuts at: 830 3272 830 Size: 2442 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5920 PvuII CAG'CTG Cuts at: 2758 3022 3086 2758 Size: 264 64 5592 Fragments arranged by size: 5592 264 64 SacI G_AGCT'C Cuts at: 2748 2748 Size: 5920 SalI G'TCGA_C Cuts at: 1936 2552 3262 1936 Size: 616 710 4594 Fragments arranged by size: 4594 710 616 SbfI CC_TGCA'GG Cuts at: 3272 3272 Size: 5920 ScaI AGT'ACT Cuts at: 4168 5880 4168 Size: 1712 4208 SfcI C'TryA_G Cuts at: 826 1045 3268 4403 5084 5275 826 Size: 219 2223 1135 681 191 1471 Fragments arranged by size: 2223 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5920 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5920 SmaI CCC'GGG Cuts at: 1872 3247 1872 Size: 1375 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2173 4024 4925 5187 820 Size: 197 926 230 1851 901 262 1553 Fragments arranged by size: 1851 1553 926 901 262 230 197 SnaBI TAC'GTA Cuts at: 493 3711 493 Size: 3218 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 5920 SphI G_CATG'C Cuts at: 1132 3278 1132 Size: 2146 3774 SspI AAT'ATT Cuts at: 5 52 3844 5 Size: 47 3792 2081 Fragments arranged by size: 3792 2081 47 StuI AGG'CCT Cuts at: 1517 2877 1517 Size: 1360 4560 StyI C'CwwG_G Cuts at: 513 1065 2070 2217 2382 3359 513 Size: 552 1005 147 165 977 3074 Fragments arranged by size: 3074 1005 977 552 165 147 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 3023 3947 3964 4117 4302 4747 325 Size: 1255 1443 924 17 153 185 445 1498 Fragments arranged by size: 1498 1443 1255 924 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2019 2901 4166 Size: 274 80 33 51 1443 42 882 1265 Cuts at: 4166 5878 96 Size: 1712 138 Fragments arranged by size: 1712 1443 1265 882 274 138 80 51 42 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 5880 5912 5880 Size: 32 5888 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 5920 XbaI T'CTAG_A Cuts at: 3256 3256 Size: 5920 XcmI CCAnnnn_n'nnnnTGG Cuts at: 2590 2890 2590 Size: 300 5620 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5920 XmaI C'CCGG_G Cuts at: 1870 3245 1870 Size: 1375 4545 XmnI GAAnn'nnTTC Cuts at: 2192 4049 2192 Size: 1857 4063 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3117 3728 276 Size: 53 83 186 936 1583 611 2468 Fragments arranged by size: 2468 1583 936 611 186 83 53 Enzymes that do cut: AarI AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaI ApaLI ApoI AseI AsiSI AvaI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DraIII DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PshAI PsiI PspOMI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XcmI XhoI XmaI XmnI ZraI Enzymes that do not cut: AfeI AloI AscI AvrII BaeI BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PpuMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI