Restriction map for
pFN21AB8709
(Linear) (Six-base) MAPSORT of: KIBB8709.seq Check: 2115 from: 1 to: 1215
KIBB8709.orf
With 182 enzymes: SgfI *
March 3, 2008 11:07 ..
AarI CACCTGCnnnn'nnnn_
Cuts at: 0 341 1215
Size: 341 874
AatII G_ACGT'C
Cuts at: 0 1114 1215
Size: 1114 101
AccI GT'mk_AC
Cuts at: 0 548 1215
Size: 548 667
AclI AA'CG_TT
Cuts at: 0 128 1215
Size: 128 1087
AcuI CTGAAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 126 1039 1215
Size: 104 22 913 176
Fragments arranged by size:
913 176 104 22
AlwNI CAG_nnn'CTG
Cuts at: 0 276 333 1215
Size: 276 57 882
Fragments arranged by size:
882 276 57
ApaI G_GGCC'C
Cuts at: 0 261 1215
Size: 261 954
ApoI r'AATT_y
Cuts at: 0 474 987 1215
Size: 474 513 228
Fragments arranged by size:
513 474 228
BamHI G'GATC_C
Cuts at: 0 69 1215
Size: 69 1146
BanI G'GyrC_C
Cuts at: 0 238 690 1215
Size: 238 452 525
Fragments arranged by size:
525 452 238
BanII G_rGCy'C
Cuts at: 0 261 743 1215
Size: 261 482 472
Fragments arranged by size:
482 472 261
BbsI GAAGACnn'nnnn_
Cuts at: 0 16 780 1215
Size: 16 764 435
Fragments arranged by size:
764 435 16
Bme1580I G_kGCm'C
Cuts at: 0 241 261 695 1215
Size: 241 20 434 520
Fragments arranged by size:
520 434 241 20
BmrI ACTGGGnnnn_n'
Cuts at: 0 495 1100 1204 1215
Size: 495 605 104 11
Fragments arranged by size:
605 495 104 11
BplI GAGnnnnnCTCnnnnnnnn_nnnnn'
Cuts at: 0 444 1215
Size: 444 771
BpmI CTGGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 253 430 439 941 967 1197 1215
Size: 253 177 9 502 26 230 18
Fragments arranged by size:
502 253 230 177 26 18 9
Bpu10I CC'TnA_GC
Cuts at: 0 219 1215
Size: 219 996
BpuEI CTTGAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 189 1215
Size: 189 1026
BsaAI yAC'GTr
Cuts at: 0 771 1215
Size: 771 444
BsaHI Gr'CG_yC
Cuts at: 0 1010 1111 1215
Size: 1010 101 104
Fragments arranged by size:
1010 104 101
BsaWI w'CCGG_w
Cuts at: 0 801 1215
Size: 801 414
BseRI GAGGAGnnnnnnnn_nn'
Cuts at: 0 667 1078 1215
Size: 667 411 137
Fragments arranged by size:
667 411 137
BseYI C'CCAG_C
Cuts at: 0 260 573 1013 1215
Size: 260 313 440 202
Fragments arranged by size:
440 313 260 202
BsgI GTGCAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 319 778 818 1215
Size: 319 459 40 397
Fragments arranged by size:
459 397 319 40
BsiHKAI G_wGCw'C
Cuts at: 0 715 743 1215
Size: 715 28 472
Fragments arranged by size:
715 472 28
Bsp1286I G_dGCh'C
Cuts at: 0 241 261 695 715 743 1215
Size: 241 20 434 20 28 472
Fragments arranged by size:
472 434 241 28 20 20
BspEI T'CCGG_A
Cuts at: 0 801 1215
Size: 801 414
BspHI T'CATG_A
Cuts at: 0 729 1154 1215
Size: 729 425 61
Fragments arranged by size:
729 425 61
BspMI ACCTGCnnnn'nnnn_
Cuts at: 0 341 1215
Size: 341 874
BsrDI GCAATG_nn'
Cuts at: 0 390 783 959 1215
Size: 390 393 176 256
Fragments arranged by size:
393 390 256 176
BsrGI T'GTAC_A
Cuts at: 0 14 896 1215
Size: 14 882 319
Fragments arranged by size:
882 319 14
BstEII G'GTnAC_C
Cuts at: 0 909 1095 1215
Size: 909 186 120
Fragments arranged by size:
909 186 120
BstYI r'GATC_y
Cuts at: 0 69 311 1215
Size: 69 242 904
Fragments arranged by size:
904 242 69
BtgI C'CryG_G
Cuts at: 0 65 212 377 1215
Size: 65 147 165 838
Fragments arranged by size:
838 165 147 65
BtgZI GCGATGnnnnnnnnnn'nnnn_
Cuts at: 0 813 1215
Size: 813 402
DraIII CAC_nnn'GTG
Cuts at: 0 162 1215
Size: 162 1053
EaeI y'GGCC_r
Cuts at: 0 246 1215
Size: 246 969
EcoICRI GAG'CTC
Cuts at: 0 741 1215
Size: 741 474
Eco57MI CTGrAGnnnnnnnnnnnnnn_nn'
Cuts at: 0 104 126 253 430 439 941 967 1039
Size: 104 22 127 177 9 502 26 72
Cuts at: 1039 1197 1215
Size: 158 18
Fragments arranged by size:
502 177 158 127 104 72 26 22
18 9
EcoO109I rG'GnC_Cy
Cuts at: 0 111 273 1215
Size: 111 162 942
Fragments arranged by size:
942 162 111
Hin4I GAynnnnnvTCnnnnnnnn_nnnnn'
Cuts at: 0 87 119 1215
Size: 87 32 1096
Fragments arranged by size:
1096 87 32
HincII GTy'rAC
Cuts at: 0 132 549 594 1215
Size: 132 417 45 621
Fragments arranged by size:
621 417 132 45
MscI TGG'CCA
Cuts at: 0 248 1215
Size: 248 967
MslI CAynn'nnrTG
Cuts at: 0 211 449 1153 1215
Size: 211 238 704 62
Fragments arranged by size:
704 238 211 62
MspA1I CmG'CkG
Cuts at: 0 44 753 1017 1081 1215
Size: 44 709 264 64 134
Fragments arranged by size:
709 264 134 64 44
NcoI C'CATG_G
Cuts at: 0 65 212 377 1215
Size: 65 147 165 838
Fragments arranged by size:
838 165 147 65
PasI CC'CwG_GG
Cuts at: 0 234 1215
Size: 234 981
PflMI CCAn_nnn'nTGG
Cuts at: 0 66 276 1215
Size: 66 210 939
Fragments arranged by size:
939 210 66
PfoI T'CCnGG_A
Cuts at: 0 479 1215
Size: 479 736
PspOMI G'GGCC_C
Cuts at: 0 257 1215
Size: 257 958
PvuII CAG'CTG
Cuts at: 0 753 1017 1081 1215
Size: 753 264 64 134
Fragments arranged by size:
753 264 134 64
SacI G_AGCT'C
Cuts at: 0 743 1215
Size: 743 472
SalI G'TCGA_C
Cuts at: 0 547 1215
Size: 547 668
SmlI C'TyrA_G
Cuts at: 0 168 1215
Size: 168 1047
StuI AGG'CCT
Cuts at: 0 872 1215
Size: 872 343
StyI C'CwwG_G
Cuts at: 0 65 212 377 1215
Size: 65 147 165 838
Fragments arranged by size:
838 165 147 65
TaqII GACCGAnnnnnnnnn_nn'
Cuts at: 0 1018 1215
Size: 1018 197
TatI w'GTAC_w
Cuts at: 0 14 896 1215
Size: 14 882 319
Fragments arranged by size:
882 319 14
XcmI CCAnnnn_n'nnnnTGG
Cuts at: 0 585 885 1215
Size: 585 300 330
Fragments arranged by size:
585 330 300
XmnI GAAnn'nnTTC
Cuts at: 0 187 1215
Size: 187 1028
ZraI GAC'GTC
Cuts at: 0 1112 1215
Size: 1112 103
Enzymes that do cut:
AarI AatII AccI AclI AcuI AlwNI ApaI ApoI
BamHI BanI BanII BbsI Bme1580I BmrI BplI BpmI
Bpu10I BpuEI BsaAI BsaHI BsaWI BseRI BseYI BsgI
BsiHKAI Bsp1286I BspEI BspHI BspMI BsrDI BsrGI BstEII
BstYI BtgI BtgZI DraIII EaeI EcoICRI Eco57MI EcoO109I
Hin4I HincII MscI MslI MspA1I NcoI PasI PflMI
PfoI PspOMI PvuII SacI SalI SmlI StuI StyI
TaqII TatI XcmI XmnI ZraI
Enzymes that do not cut:
Acc65I AfeI AflII AflIII AgeI AhdI AleI AlfI
AloI ApaLI AscI AseI AsiSI AvaI AvrII BaeI
BbeI BbvCI BcgI BciVI BclI BfrBI BglI BglII
BlpI BmgBI BmtI BsaI BsaBI BsaXI BsiEI BsiWI
BsmI BsmBI BsrBI BsrFI BssHII BssSI BstAPI BstBI
BstXI BstZ17I Bsu36I BtsI ClaI CspCI DraI DrdI
EagI EarI EciI EcoNI EcoRI EcoRV FalI FseI
FspI FspAI HaeII HindIII HpaI KasI KpnI MfeI
MluI MmeI NaeI NarI NdeI NgoMIV NheI NotI
NruI NsiI NspI PacI PciI PmeI PmlI PpiI
PpuMI PshAI PsiI PspXI PsrI PstI PvuI RsrII
SacII SanDI SapI SbfI ScaI SexAI SfcI SfiI
SfoI SgfI SgrAI SmaI SnaBI SpeI SphI SrfI
SspI SwaI TstI Tth111I XbaI XhoI XmaI