(Circular) (Six-base) MAPSORT of: pFN21AB8706.seq Check: 6816 from: 1 to: 6175 pFN21AB8706 6175 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3985 278 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3518 1937 Size: 1581 4594 Acc65I G'GTAC_C Cuts at: 3496 3496 Size: 6175 AclI AA'CG_TT Cuts at: 1563 4302 4675 1563 Size: 2739 373 3063 Fragments arranged by size: 3063 2739 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 2493 2844 4238 5622 5890 1632 Size: 861 351 1394 1384 268 1917 Fragments arranged by size: 1917 1394 1384 861 351 268 AflII C'TTAA_G Cuts at: 820 1017 2828 820 Size: 197 1811 4167 Fragments arranged by size: 4167 1811 197 AflIII A'CryG_T Cuts at: 1203 5074 6051 1203 Size: 3871 977 1327 Fragments arranged by size: 3871 1327 977 AgeI A'CCGG_T Cuts at: 5006 5006 Size: 6175 AhdI GACnn_n'nnGTC Cuts at: 2023 4904 2023 Size: 2881 3294 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 6175 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 6175 AlwNI CAG_nnn'CTG Cuts at: 3918 5490 3918 Size: 1572 4603 ApaLI G'TGCA_C Cuts at: 4232 5388 4232 Size: 1156 5019 ApoI r'AATT_y Cuts at: 1493 2712 2875 3283 3350 3484 3710 3862 5971 Size: 1219 163 408 67 134 226 152 2109 Cuts at: 5971 1493 Size: 1697 Fragments arranged by size: 2109 1697 1219 408 226 163 152 134 67 AseI AT'TA_AT Cuts at: 160 1032 4729 160 Size: 872 3697 1606 Fragments arranged by size: 3697 1606 872 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 AvaI C'yCGr_G Cuts at: 1870 1943 3500 1870 Size: 73 1557 4545 Fragments arranged by size: 4545 1557 73 AvrII C'CTAG_G Cuts at: 3388 3388 Size: 6175 BamHI G'GATC_C Cuts at: 3505 3872 3505 Size: 367 5808 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3496 4951 618 Size: 325 217 627 1709 1455 1842 Fragments arranged by size: 1842 1709 1455 627 325 217 BanII G_rGCy'C Cuts at: 1391 2440 3200 3494 1391 Size: 1049 760 294 4072 Fragments arranged by size: 4072 1049 760 294 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 2703 2760 928 Size: 971 804 57 4343 Fragments arranged by size: 4343 971 804 57 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 4366 4400 1903 Size: 34 2429 34 3678 Fragments arranged by size: 3678 2429 34 34 BciVI GTATCCnnnnn_n' Cuts at: 2895 4067 5277 2895 Size: 1172 1210 3793 Fragments arranged by size: 3793 1210 1172 BclI T'GATC_A Cuts at: 1548 1548 Size: 6175 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 4786 136 Size: 107 122 71 631 605 3114 1525 Fragments arranged by size: 3114 1525 631 605 122 107 71 BglII A'GATC_T Cuts at: 6170 6170 Size: 6175 BlpI GC'TnA_GC Cuts at: 3592 3592 Size: 6175 Bme1580I G_kGCm'C Cuts at: 1790 4236 5392 1790 Size: 2446 1156 2573 Fragments arranged by size: 2573 2446 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 6175 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3236 3906 4864 Size: 416 53 83 399 24 1819 670 958 Cuts at: 4864 442 Size: 1753 Fragments arranged by size: 1819 1753 958 670 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 6175 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 3219 3219 Size: 6175 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2729 4835 1239 Size: 1490 2106 2579 Fragments arranged by size: 2579 2106 1490 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 4300 5165 5463 4300 Size: 865 298 5012 Fragments arranged by size: 5012 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4838 882 Size: 617 3339 2219 Fragments arranged by size: 3339 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3966 493 Size: 711 2762 2702 Fragments arranged by size: 2762 2702 711 BsaBI GATnn'nnATC Cuts at: 6169 6169 Size: 6175 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3982 4364 4999 275 Size: 53 83 186 936 2449 382 635 1451 Fragments arranged by size: 2449 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4608 5006 5280 5427 5838 4608 Size: 398 274 147 411 4945 Fragments arranged by size: 4945 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5950 Fragments arranged by size: 5950 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 1186 Size: 6175 BseYI C'CCAG_C Cuts at: 2219 3277 5378 5874 2219 Size: 1058 2101 496 2520 Fragments arranged by size: 2520 2101 1058 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 1163 Size: 573 5602 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3625 4386 5414 1540 Size: 275 186 1624 761 1028 2301 Fragments arranged by size: 2301 1624 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2198 3200 4236 4321 5392 2198 Size: 1002 1036 85 1071 2981 Fragments arranged by size: 2981 1071 1036 1002 85 BsmI GAATG_Cn' Cuts at: 3686 3779 3686 Size: 93 6082 BsmBI CGTCTCn'nnnn_ Cuts at: 2743 2743 Size: 6175 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2198 2440 3200 3494 4236 4321 5392 Size: 399 408 242 760 294 742 85 1071 Cuts at: 5392 1391 Size: 2174 Fragments arranged by size: 2174 1071 760 742 408 399 294 242 85 BspEI T'CCGG_A Cuts at: 5838 5838 Size: 6175 BspHI T'CATG_A Cuts at: 4064 5794 4064 Size: 1730 4445 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3530 844 Size: 2686 3489 BsrBI CCG'CTC Cuts at: 1394 1823 4062 1394 Size: 429 2239 3507 Fragments arranged by size: 3507 2239 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2263 2985 4670 4844 65 Size: 1183 1015 722 1685 174 1396 Fragments arranged by size: 1685 1396 1183 1015 722 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4819 5006 1700 Size: 110 3009 187 2869 Fragments arranged by size: 3009 2869 187 110 BsrGI T'GTAC_A Cuts at: 96 2627 96 Size: 2531 3644 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 6175 BssSI C'ACGA_G Cuts at: 4235 5247 4235 Size: 1012 5163 BstAPI GCAn_nnn'nTGC Cuts at: 794 794 Size: 6175 BstBI TT'CG_AA Cuts at: 4979 4979 Size: 6175 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 6175 BstYI r'GATC_y Cuts at: 1972 3505 3872 4257 4274 5726 5812 5824 6170 Size: 1533 367 385 17 1452 86 12 346 Cuts at: 6170 1972 Size: 1977 Fragments arranged by size: 1977 1533 1452 385 367 346 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2004 513 Size: 552 939 4684 Fragments arranged by size: 4684 939 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5436 Fragments arranged by size: 5436 683 56 BtsI GCAGTG_nn' Cuts at: 2217 3691 4485 4505 2217 Size: 1474 794 20 3887 Fragments arranged by size: 3887 1474 794 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 6175 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 6140 DraI TTT'AAA Cuts at: 3479 3479 Size: 6175 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 5182 809 Size: 507 3866 1802 Fragments arranged by size: 3866 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 3622 4511 6140 Size: 54 1425 50 275 18 1792 889 1629 Cuts at: 6140 8 Size: 43 Fragments arranged by size: 1792 1629 1425 889 275 54 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3622 1537 Size: 275 1810 4090 Fragments arranged by size: 4090 1810 275 EarI CTCTTCn'nnn_ Cuts at: 1353 4105 1353 Size: 2752 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4762 5136 5282 387 Size: 1064 138 3173 374 146 1280 Fragments arranged by size: 3173 1280 1064 374 146 138 EcoICRI GAG'CTC Cuts at: 3198 3198 Size: 6175 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2493 2729 2844 4238 4835 5622 5890 Size: 393 861 236 115 1394 597 787 268 Cuts at: 5890 1239 Size: 1524 Fragments arranged by size: 1524 1394 861 787 597 393 268 236 115 EcoNI CCTnn'n_nnAGG Cuts at: 2423 3006 2423 Size: 583 5592 EcoO109I rG'GnC_Cy Cuts at: 2999 3385 2999 Size: 386 5789 EcoRI G'AATT_C Cuts at: 3484 3862 3484 Size: 378 5797 FspI TGC'GCA Cuts at: 4681 4681 Size: 6175 HaeII r_GCGC'y Cuts at: 1678 5322 1678 Size: 3644 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2009 2041 3058 3090 4816 Size: 32 135 32 437 32 1017 32 1726 Cuts at: 4816 4848 4890 4922 1373 Size: 32 42 32 2626 Fragments arranged by size: 2626 1726 1017 437 135 42 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 2173 3519 3765 669 Size: 988 281 235 1346 246 3079 Fragments arranged by size: 3079 1346 988 281 246 235 HindIII A'AGCT_T Cuts at: 748 748 Size: 6175 HpaI GTT'AAC Cuts at: 3765 3765 Size: 6175 KpnI G_GTAC'C Cuts at: 3500 3500 Size: 6175 MfeI C'AATT_G Cuts at: 3774 3774 Size: 6175 MluI A'CGCG_T Cuts at: 6051 6051 Size: 6175 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2376 2778 5289 5473 1305 Size: 1071 402 2511 184 2007 Fragments arranged by size: 2511 2007 1071 402 184 MscI TGG'CCA Cuts at: 10 64 1489 1832 10 Size: 54 1425 343 4353 Fragments arranged by size: 4353 1425 343 54 MslI CAynn'nnrTG Cuts at: 518 1166 2660 3152 4133 4492 4651 518 Size: 648 1494 492 981 359 159 2042 Fragments arranged by size: 2042 1494 981 648 492 359 159 MspA1I CmG'CkG Cuts at: 1606 2219 2891 3591 4268 5416 5661 1606 Size: 613 672 700 677 1148 245 2120 Fragments arranged by size: 2120 1148 700 677 672 613 245 NcoI C'CATG_G Cuts at: 513 1065 2004 513 Size: 552 939 4684 Fragments arranged by size: 4684 939 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 6175 NheI G'CTAG_C Cuts at: 1052 1052 Size: 6175 NotI GC'GGCC_GC Cuts at: 3622 3622 Size: 6175 NspI r_CATG'y Cuts at: 1132 1229 3533 5078 1132 Size: 97 2304 1545 2229 Fragments arranged by size: 2304 2229 1545 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 6175 PciI A'CATG_T Cuts at: 5074 5074 Size: 6175 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 6175 PmeI GTTT'AAAC Cuts at: 3479 3479 Size: 6175 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 4244 4276 5757 5789 4244 Size: 32 1481 32 4630 Fragments arranged by size: 4630 1481 32 32 PshAI GACnn'nnGTC Cuts at: 1613 2456 1613 Size: 843 5332 PsiI TTA'TAA Cuts at: 3745 3745 Size: 6175 PstI C_TGCA'G Cuts at: 830 3364 3527 830 Size: 2534 163 3478 Fragments arranged by size: 3478 2534 163 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 6175 PvuII CAG'CTG Cuts at: 2219 2219 Size: 6175 SacI G_AGCT'C Cuts at: 3200 3200 Size: 6175 SalI G'TCGA_C Cuts at: 1936 3517 1936 Size: 1581 4594 SbfI CC_TGCA'GG Cuts at: 3527 3527 Size: 6175 ScaI AGT'ACT Cuts at: 4423 6135 4423 Size: 1712 4463 SfcI C'TryA_G Cuts at: 826 1045 3103 3360 3523 4658 5339 5530 826 Size: 219 2058 257 163 1135 681 191 1471 Fragments arranged by size: 2058 1471 1135 681 257 219 191 163 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 6175 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 6175 SmaI CCC'GGG Cuts at: 1872 3502 1872 Size: 1630 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 2828 4279 5180 5442 820 Size: 197 926 885 1451 901 262 1553 Fragments arranged by size: 1553 1451 926 901 885 262 197 SnaBI TAC'GTA Cuts at: 493 3966 493 Size: 3473 2702 SpeI A'CTAG_T Cuts at: 152 152 Size: 6175 SphI G_CATG'C Cuts at: 1132 3533 1132 Size: 2401 3774 SspI AAT'ATT Cuts at: 5 52 2950 4099 5 Size: 47 2898 1149 2081 Fragments arranged by size: 2898 2081 1149 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 6175 StyI C'CwwG_G Cuts at: 513 1065 2004 3388 3614 513 Size: 552 939 1384 226 3074 Fragments arranged by size: 3074 1384 939 552 226 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 4202 4219 4372 4557 5002 325 Size: 1255 2622 17 153 185 445 1498 Fragments arranged by size: 2622 1498 1255 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 2627 4421 6133 Size: 274 80 33 51 1443 650 1794 1712 Cuts at: 6133 96 Size: 138 Fragments arranged by size: 1794 1712 1443 650 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 3162 3194 6135 6167 3162 Size: 32 2941 32 3170 Fragments arranged by size: 3170 2941 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 1316 Size: 6175 XbaI T'CTAG_A Cuts at: 3511 3511 Size: 6175 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 6175 XmaI C'CCGG_G Cuts at: 1870 3500 1870 Size: 1630 4545 XmnI GAAnn'nnTTC Cuts at: 4304 4304 Size: 6175 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3983 276 Size: 53 83 186 936 2449 2468 Fragments arranged by size: 2468 2449 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AlwNI ApaLI ApoI AseI AsiSI AvaI AvrII BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI BsmBI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI EcoICRI Eco57MI EcoNI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PmeI PpiI PshAI PsiI PstI PvuI PvuII SacI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI AloI ApaI AscI BaeI BbeI BbvCI BfrBI Bpu10I BsiWI BstXI BstZ17I Bsu36I DraIII EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PfoI PmlI PpuMI PspOMI PspXI PsrI RsrII SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI