Restriction map for
pFN21AB8705
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# NO CUT ENZYMES ( 161 enzymes) #
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AatII Acc65I AccI AccII AccIII
AclI AcyI AflII AgeI AhdI
Alw44I AocI Aor51HI ApaI ApaLI
AscI Asp700I Asp718I AspEI AviII
AvrII BamHI BanI BanII BbeI
BbrPI BbsI BbvCI BciVI BfrI
BlnI BlpI Bpu1102I BpuAI BsaBI
BsaHI BsaI BsaWI BseAI BsiEI
BsiWI BsmBI BsmI Bsp1407I BspDI
BspEI BspMI BsrFI BsrGI BssHII
BssSI Bst1107I BstBI BstEII BstF5I
BstNI BstPI BstUI BstXI BstZ17I
Bsu36I CelII CfoI Cfr10I ClaI
CpoI DraI DraIII DrdI EagI
Eam1105I EarI EclXI Eco47III Eco52I
Eco81I EcoNI EcoO65I EcoRI EcoRII
EcoRV EcoT14I EcoT22I FauI FokI
FseI FspI HaeII HgaI HhaI
Hin1I HinP1I HincII HindII HindIII
HpaI HphI KasI KpnI Ksp632I
KspI MaeIII MamI MfeI MluI
MroI MspA1I MunI MvaI MvnI
NaeI NarI NcoI NdeI NgoMIV
NheI NotI NruI NsiI NspV
PacI PaeR7I PflMI PinAI PmaCI
PmeI PmlI PshAI Psp1406I PstI
PvuI PvuII RsrII SacI SacII
SalI SapI SbfI ScaI SexAI
SfcI SfiI SfoI SfuI SgrAI
SmaI SmlI SpeI SphI Sse8387I
SspBI StuI StyI SwaI Tsp45I
Van91I XcmI XhoI XmaCI XmaI
XmnI
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# ONE CUT ENZYMES ( 46 enzymes) #
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AciI 307 GCGG CCGC(-3/-1)
AcsI 777 AAATTT [GA]^AATT[CT]
AflIII 209 ACATGT A^C[GA][CT]GT
AlwI 799 GATCC GGATC(4/5)
AlwNI 248 CAGCTTCTG CAG[ACGT][ACGT][ACGT]^CTG
ApoI 777 AAATTT [GA]^AATT[CT]
AspHI 542 GTGCTC G[AT]GC[AT]^C
AspI 518 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
AvaI 66 CTCGGG C^[CT]CG[GA]G
BcgI 219 CGATAGCACTGC (10/12)CGA[ACGT][ACGT][ACGT][ACGT][ACGT][ACGT]TGC(12/10)
BclI 618 TGATCA T^GATCA
BcnI 104 CCCGG CC^[GC]GG
BglI 229 GCCATTTTGGC GCC[ACGT][ACGT][ACGT][ACGT]^[ACGT]GGC
BglII 62 AGATCT A^GATCT
BmyI 542 GTGCTC G[AGT]GC[ACT]^C
BsaAI 1102 TACGTA [CT]AC^GT[GA]
BsaJI 513 CCACGG C^C[ACGT][ACGT]GG
BseRI 890 CTCCTC GAGGAG(10/8)
BsgI 605 CTGCAC GTGCAG(16/14)
BsiHKAI 542 GTGCTC G[AT]GC[AT]^C
BsiYI 434 CCATTCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BslI 434 CCATTCTTGGG CC[ACGT][ACGT][ACGT][ACGT][ACGT]^[ACGT][ACGT]GG
BsmFI 1006 GGGAC GGGAC(10/14)
BsoBI 66 CTCGGG C^[CT]CG[GA]G
Bsp1286I 542 GTGCTC G[AGT]GC[ACT]^C
BspLU11I 209 ACATGT A^CATGT
BsrBI 305 GAGCGG CCGCTC(-3/-3)
BsrDI 574 CATTGC GCAATG(2/0)
BsrI 243 ACTGG ACTGG(1/-1)
BssKI 104 CCCGG ^CC[ACGT]GG
BstAPI 1027 GCACCTGATGC GCA[ACGT][ACGT][ACGT][ACGT]^[ACGT]TGC
Cac8I 319 GCTGGC GC[ACGT]^[ACGT]GC
DsaI 513 CCACGG C^C[GA][CT]GG
FbaI 618 TGATCA T^GATCA
HapII 105 CCGG C^CGG
HpaII 105 CCGG C^CGG
MspI 105 CCGG C^CGG
NciI 104 CCCGG CC^[GC]GG
NspI 209 ACATGT [GA]CATG^[CT]
PflFI 518 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
PleI 199 GACTC GAGTC(4/5)
ScrFI 104 CCCGG CC^[ACGT]GG
SnaBI 1102 TACGTA TAC^GTA
TfiI 862 GAATC G^A[AT]TC
Tth111I 518 GACACAGTC GAC[ACGT]^[ACGT][ACGT]GTC
XbaI 546 TCTAGA T^CTAGA
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# TWO CUT ENZYMES ( 25 enzymes) #
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AfaI 934 GTAC GT^AC
1105 GTAC
AluI 249 AGCT AG^CT
1097 AGCT
AseI 876 ATTAAT AT^TAAT
1017 ATTAAT
AsnI 876 ATTAAT AT^TAAT
1017 ATTAAT
AvaII 682 GGACC G^G[AT]CC
1007 GGACC
BalI 510 TGGCCA TGG^CCA
561 TGGCCA
BsmAI 417 GAGAC GTCTC(1/5)
1092 GAGAC
BspHI 651 TCATGA T^CATGA
767 TCATGA
BstYI 62 AGATCT [GA]^GATC[CT]
798 AGATCC
DraII 681 AGGACCC [GA]G^G[ACGT]CC[CT]
1006 GGGACCT
EaeI 510 TGGCCA [CT]^GGCC[GA]
561 TGGCCA
EcoO109I 681 AGGACCC [GA]G^G[ACGT]CC[CT]
1006 GGGACCT
HinfI 199 GACTC G^A[ACGT]TC
862 GAATC
MaeII 995 ACGT A^CGT
1103 ACGT
MboII 119 TCTTC GAAGA(8/7)
347 TCTTC
MflI 62 AGATCT [GA]^GATC[CT]
798 AGATCC
MluNI 510 TGGCCA TGG^CCA
561 TGGCCA
MscI 510 TGGCCA TGG^CCA
561 TGGCCA
PpuMI 681 AGGACCC [GA]G^G[AT]CC[CT]
1006 GGGACCT
PshBI 876 ATTAAT AT^TAAT
1017 ATTAAT
RcaI 651 TCATGA T^CATGA
767 TCATGA
RsaI 934 GTAC GT^AC
1105 GTAC
TaqI 218 TCGA T^CGA
569 TCGA
TthHB8I 218 TCGA T^CGA
569 TCGA
XhoII 62 AGATCT [GA]^GATC[CT]
798 AGATCC
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# 3 OR MORE CUT ENZYMES ( 26 enzymes) #
#########################################
BbvI BfaI BpmI Cfr13I DdeI
DpnII Fnu4HI HaeIII ItaI MaeI
MboI MnlI MseI MslI MwoI
NdeII NlaIII NlaIV Sau3AI Sau96I
SfaNI SspI Tru9I TseI Tsp509I
TspRI