(Circular) (Six-base) MAPSORT of: pFN21AB8705.seq Check: 4527 from: 1 to: 5833 pFN21AB8705 5833 bp With 182 enzymes: SgfI * January 16, 2009 15:27 .. AatII G_ACGT'C Cuts at: 278 331 414 600 1536 3643 278 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 AccI GT'mk_AC Cuts at: 1937 3176 1937 Size: 1239 4594 Acc65I G'GTAC_C Cuts at: 3154 3154 Size: 5833 AclI AA'CG_TT Cuts at: 1563 3960 4333 1563 Size: 2397 373 3063 Fragments arranged by size: 3063 2397 373 AcuI CTGAAGnnnnnnnnnnnnnn_nn' Cuts at: 1632 3896 5280 5548 1632 Size: 2264 1384 268 1917 Fragments arranged by size: 2264 1917 1384 268 AflII C'TTAA_G Cuts at: 820 1017 820 Size: 197 5636 AflIII A'CryG_T Cuts at: 1203 2214 4732 5709 1203 Size: 1011 2518 977 1327 Fragments arranged by size: 2518 1327 1011 977 AgeI A'CCGG_T Cuts at: 4664 4664 Size: 5833 AhdI GACnn_n'nnGTC Cuts at: 4562 4562 Size: 5833 AleI CACnn'nnGTG Cuts at: 1166 1166 Size: 5833 AlfI GCAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1184 1184 Size: 5833 AloI GAACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2977 3009 2977 Size: 32 5801 AlwNI CAG_nnn'CTG Cuts at: 2258 3576 5148 2258 Size: 1318 1572 2943 Fragments arranged by size: 2943 1572 1318 ApaLI G'TGCA_C Cuts at: 3890 5046 3890 Size: 1156 4677 ApoI r'AATT_y Cuts at: 1493 2782 3142 3368 3520 5629 1493 Size: 1289 360 226 152 2109 1697 Fragments arranged by size: 2109 1697 1289 360 226 152 AseI AT'TA_AT Cuts at: 160 1032 2882 3023 4387 160 Size: 872 1850 141 1364 1606 Fragments arranged by size: 1850 1606 1364 872 141 AsiSI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 AvaI C'yCGr_G Cuts at: 1870 1943 2071 3158 1870 Size: 73 128 1087 4545 Fragments arranged by size: 4545 1087 128 73 BaeI ACnnnnGTAyCnnnnnnn_nnnnn' Cuts at: 2930 2963 2930 Size: 33 5800 BamHI G'GATC_C Cuts at: 3163 3530 3163 Size: 367 5466 BanI G'GyrC_C Cuts at: 618 943 1160 1787 3154 4609 618 Size: 325 217 627 1367 1455 1842 Fragments arranged by size: 1842 1455 1367 627 325 217 BanII G_rGCy'C Cuts at: 1391 3152 1391 Size: 1761 4072 BbsI GAAGACnn'nnnn_ Cuts at: 928 1899 928 Size: 971 4862 BcgI CGAnnnnnnTGCnnnnnnnnnn_nn' Cuts at: 1903 1937 2213 2247 4024 4058 1903 Size: 34 276 34 1777 34 3678 Fragments arranged by size: 3678 1777 276 34 34 34 BciVI GTATCCnnnnn_n' Cuts at: 3725 4935 3725 Size: 1210 4623 BclI T'GATC_A Cuts at: 1548 2623 1548 Size: 1075 4758 BglI GCCn_nnn'nGGC Cuts at: 136 243 365 436 1067 1672 2240 4444 136 Size: 107 122 71 631 605 568 2204 1525 Fragments arranged by size: 2204 1525 631 605 568 122 107 71 BglII A'GATC_T Cuts at: 2067 5828 2067 Size: 3761 2072 BlpI GC'TnA_GC Cuts at: 3250 3250 Size: 5833 Bme1580I G_kGCm'C Cuts at: 1790 3894 5050 1790 Size: 2104 1156 2573 Fragments arranged by size: 2573 2104 1156 BmgBI CAC'GTC Cuts at: 1318 1318 Size: 5833 BmrI ACTGGGnnnn_n' Cuts at: 442 858 911 994 1393 1417 3564 4522 442 Size: 416 53 83 399 24 2147 958 1753 Fragments arranged by size: 2147 1753 958 416 399 83 53 24 BmtI G_CTAG'C Cuts at: 1056 1056 Size: 5833 BplI GAGnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2913 2913 Size: 5833 BpmI CTGGAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 2197 2388 3111 4493 1239 Size: 958 191 723 1382 2579 Fragments arranged by size: 2579 1382 958 723 191 Bpu10I CC'TnA_GC Cuts at: 2188 2188 Size: 5833 BpuEI CTTGAGnnnnnnnnnnnnnn_nn' Cuts at: 3958 4823 5121 3958 Size: 865 298 4670 Fragments arranged by size: 4670 865 298 BsaI GGTCTCn'nnnn_ Cuts at: 882 1499 4496 882 Size: 617 2997 2219 Fragments arranged by size: 2997 2219 617 BsaAI yAC'GTr Cuts at: 493 1204 3109 3624 493 Size: 711 1905 515 2702 Fragments arranged by size: 2702 1905 711 515 BsaBI GATnn'nnATC Cuts at: 5827 5827 Size: 5833 BsaHI Gr'CG_yC Cuts at: 275 328 411 597 1533 3640 4022 4657 275 Size: 53 83 186 936 2107 382 635 1451 Fragments arranged by size: 2107 1451 936 635 382 186 83 53 BsaWI w'CCGG_w Cuts at: 4266 4664 4938 5085 5496 4266 Size: 398 274 147 411 4603 Fragments arranged by size: 4603 411 398 274 147 BsaXI ACnnnnnCTCCnnnnnnn_nnn' Cuts at: 1178 1208 1373 1403 1178 Size: 30 165 30 5608 Fragments arranged by size: 5608 165 30 30 BseRI GAGGAGnnnnnnnn_nn' Cuts at: 1186 2886 1186 Size: 1700 4133 BseYI C'CCAG_C Cuts at: 2690 5036 5532 2690 Size: 2346 496 2991 Fragments arranged by size: 2991 2346 496 BsgI GTGCAGnnnnnnnnnnnnnn_nn' Cuts at: 1163 1736 2595 1163 Size: 573 859 4401 Fragments arranged by size: 4401 859 573 BsiEI CG_ry'CG Cuts at: 1540 1815 2001 3283 4044 5072 1540 Size: 275 186 1282 761 1028 2301 Fragments arranged by size: 2301 1282 1028 761 275 186 BsiHKAI G_wGCw'C Cuts at: 2551 3894 3979 5050 2551 Size: 1343 85 1071 3334 Fragments arranged by size: 3334 1343 1071 85 BsmI GAATG_Cn' Cuts at: 3344 3437 3344 Size: 93 5740 Bsp1286I G_dGCh'C Cuts at: 1391 1790 2551 3152 3894 3979 5050 1391 Size: 399 761 601 742 85 1071 2174 Fragments arranged by size: 2174 1071 761 742 601 399 85 BspEI T'CCGG_A Cuts at: 5496 5496 Size: 5833 BspHI T'CATG_A Cuts at: 2656 2772 3722 5452 2656 Size: 116 950 1730 3037 Fragments arranged by size: 3037 1730 950 116 BspMI ACCTGCnnnn'nnnn_ Cuts at: 844 3188 844 Size: 2344 3489 BsrBI CCG'CTC Cuts at: 1394 1823 2312 3720 1394 Size: 429 489 1408 3507 Fragments arranged by size: 3507 1408 489 429 BsrDI GCAATG_nn' Cuts at: 65 1248 2578 4328 4502 65 Size: 1183 1330 1750 174 1396 Fragments arranged by size: 1750 1396 1330 1183 174 BsrFI r'CCGG_y Cuts at: 1700 1810 4477 4664 1700 Size: 110 2667 187 2869 Fragments arranged by size: 2869 2667 187 110 BsrGI T'GTAC_A Cuts at: 96 96 Size: 5833 BssHII G'CGCG_C Cuts at: 1925 1925 Size: 5833 BssSI C'ACGA_G Cuts at: 3893 4905 3893 Size: 1012 4821 BstAPI GCAn_nnn'nTGC Cuts at: 794 3038 794 Size: 2244 3589 BstBI TT'CG_AA Cuts at: 4637 4637 Size: 5833 BstEII G'GTnAC_C Cuts at: 1184 1184 Size: 5833 BstYI r'GATC_y Cuts at: 1972 2067 2803 3163 3530 3915 3932 5384 5470 Size: 95 736 360 367 385 17 1452 86 Cuts at: 5470 5482 5828 1972 Size: 12 346 1977 Fragments arranged by size: 1977 1452 736 385 367 360 346 95 86 17 12 BtgI C'CryG_G Cuts at: 513 1065 2518 3129 513 Size: 552 1453 611 3217 Fragments arranged by size: 3217 1453 611 552 BtgZI GCGATGnnnnnnnnnn'nnnn_ Cuts at: 487 1170 1226 487 Size: 683 56 5094 Fragments arranged by size: 5094 683 56 BtsI GCAGTG_nn' Cuts at: 2229 2355 3349 4143 4163 2229 Size: 126 994 794 20 3899 Fragments arranged by size: 3899 994 794 126 20 ClaI AT'CG_AT Cuts at: 1554 1554 Size: 5833 CspCI CAAnnnnnGTGGnnnnnnnnnn_nn' Cuts at: 529 564 529 Size: 35 5798 DraI TTT'AAA Cuts at: 3137 3137 Size: 5833 DrdI GACnn_nn'nnGTC Cuts at: 809 1316 4840 809 Size: 507 3524 1802 Fragments arranged by size: 3524 1802 507 EaeI y'GGCC_r Cuts at: 8 62 1487 1537 1812 1830 2515 2566 3280 Size: 54 1425 50 275 18 685 51 714 Cuts at: 3280 4169 5798 8 Size: 889 1629 43 Fragments arranged by size: 1629 1425 889 714 685 275 54 51 50 43 18 EagI C'GGCC_G Cuts at: 1537 1812 3280 1537 Size: 275 1468 4090 Fragments arranged by size: 4090 1468 275 EarI CTCTTCn'nnn_ Cuts at: 1353 3763 1353 Size: 2410 3423 EciI GGCGGAnnnnnnnnn_nn' Cuts at: 387 1451 1589 4420 4794 4940 387 Size: 1064 138 2831 374 146 1280 Fragments arranged by size: 2831 1280 1064 374 146 138 Eco57MI CTGrAGnnnnnnnnnnnnnn_nn' Cuts at: 1239 1632 2197 2388 3111 3896 4493 5280 5548 Size: 393 565 191 723 785 597 787 268 Cuts at: 5548 1239 Size: 1524 Fragments arranged by size: 1524 787 785 723 597 565 393 268 191 EcoO109I rG'GnC_Cy Cuts at: 2687 3012 2687 Size: 325 5508 EcoRI G'AATT_C Cuts at: 3142 3520 3142 Size: 378 5455 FspI TGC'GCA Cuts at: 4339 4339 Size: 5833 HaeII r_GCGC'y Cuts at: 1678 4980 1678 Size: 3302 2531 Hin4I GAynnnnnvTCnnnnnnnn_nnnnn' Cuts at: 1373 1405 1540 1572 2276 2308 2651 2683 2788 Size: 32 135 32 704 32 343 32 105 Cuts at: 2788 2820 4474 4506 4548 4580 1373 Size: 32 1654 32 42 32 2626 Fragments arranged by size: 2626 1654 704 343 135 105 42 32 32 32 32 32 32 32 HincII GTy'rAC Cuts at: 669 1657 1938 3177 3423 669 Size: 988 281 1239 246 3079 Fragments arranged by size: 3079 1239 988 281 246 HindIII A'AGCT_T Cuts at: 748 748 Size: 5833 HpaI GTT'AAC Cuts at: 3423 3423 Size: 5833 KpnI G_GTAC'C Cuts at: 3158 3158 Size: 5833 MfeI C'AATT_G Cuts at: 3432 3432 Size: 5833 MluI A'CGCG_T Cuts at: 5709 5709 Size: 5833 MmeI TCCrACnnnnnnnnnnnnnnnnnn_nn' Cuts at: 1305 2121 2226 2854 4947 5131 1305 Size: 816 105 628 2093 184 2007 Fragments arranged by size: 2093 2007 816 628 184 105 MscI TGG'CCA Cuts at: 10 64 1489 1832 2517 2568 10 Size: 54 1425 343 685 51 3275 Fragments arranged by size: 3275 1425 685 343 54 51 MslI CAynn'nnrTG Cuts at: 518 1166 2505 2568 2589 2619 2625 2631 2637 Size: 648 1339 63 21 30 6 6 6 Cuts at: 2637 2655 2774 3791 4150 4309 518 Size: 18 119 1017 359 159 2042 Fragments arranged by size: 2042 1339 1017 648 359 159 119 63 30 21 18 6 6 6 MspA1I CmG'CkG Cuts at: 1606 3249 3926 5074 5319 1606 Size: 1643 677 1148 245 2120 Fragments arranged by size: 2120 1643 1148 677 245 NcoI C'CATG_G Cuts at: 513 1065 3129 513 Size: 552 2064 3217 Fragments arranged by size: 3217 2064 552 NdeI CA'TA_TG Cuts at: 387 387 Size: 5833 NheI G'CTAG_C Cuts at: 1052 1052 Size: 5833 NotI GC'GGCC_GC Cuts at: 3280 3280 Size: 5833 NspI r_CATG'y Cuts at: 1132 1229 2218 3191 4736 1132 Size: 97 989 973 1545 2229 Fragments arranged by size: 2229 1545 989 973 97 PasI CC'CwG_GG Cuts at: 1348 1348 Size: 5833 PciI A'CATG_T Cuts at: 2214 4732 2214 Size: 2518 3315 PflMI CCAn_nnn'nTGG Cuts at: 1108 1108 Size: 5833 PfoI T'CCnGG_A Cuts at: 2108 2108 Size: 5833 PmeI GTTT'AAAC Cuts at: 3137 3137 Size: 5833 PpiI GAACnnnnnCTCnnnnnnnn_nnnnn' Cuts at: 2301 2333 3902 3934 5415 5447 2301 Size: 32 1569 32 1481 32 2687 Fragments arranged by size: 2687 1569 1481 32 32 32 PpuMI rG'GwC_Cy Cuts at: 2687 3012 2687 Size: 325 5508 PshAI GACnn'nnGTC Cuts at: 1613 1613 Size: 5833 PsiI TTA'TAA Cuts at: 3403 3403 Size: 5833 PstI C_TGCA'G Cuts at: 830 3185 830 Size: 2355 3478 PvuI CG_AT'CG Cuts at: 2001 2001 Size: 5833 SalI G'TCGA_C Cuts at: 1936 3175 1936 Size: 1239 4594 SbfI CC_TGCA'GG Cuts at: 3185 3185 Size: 5833 ScaI AGT'ACT Cuts at: 4081 5793 4081 Size: 1712 4121 SfcI C'TryA_G Cuts at: 826 1045 3181 4316 4997 5188 826 Size: 219 2136 1135 681 191 1471 Fragments arranged by size: 2136 1471 1135 681 219 191 SgfI GCG_AT'CGC Cuts at: 2001 2001 Size: 5833 SgrAI Cr'CCGG_yG Cuts at: 1700 1700 Size: 5833 SmaI CCC'GGG Cuts at: 1872 3160 1872 Size: 1288 4545 SmlI C'TyrA_G Cuts at: 820 1017 1943 3937 4838 5100 820 Size: 197 926 1994 901 262 1553 Fragments arranged by size: 1994 1553 926 901 262 197 SnaBI TAC'GTA Cuts at: 493 3109 3624 493 Size: 2616 515 2702 Fragments arranged by size: 2702 2616 515 SpeI A'CTAG_T Cuts at: 152 152 Size: 5833 SphI G_CATG'C Cuts at: 1132 3191 1132 Size: 2059 3774 SspI AAT'ATT Cuts at: 5 52 2472 2880 3076 3757 5 Size: 47 2420 408 196 681 2081 Fragments arranged by size: 2420 2081 681 408 196 47 StuI AGG'CCT Cuts at: 1517 1517 Size: 5833 StyI C'CwwG_G Cuts at: 513 1065 3129 3272 513 Size: 552 2064 143 3074 Fragments arranged by size: 3074 2064 552 143 TaqII GACCGAnnnnnnnnn_nn' Cuts at: 325 1580 2059 3860 3877 4030 4215 4660 325 Size: 1255 479 1801 17 153 185 445 1498 Fragments arranged by size: 1801 1498 1255 479 445 185 153 17 TatI w'GTAC_w Cuts at: 96 370 450 483 534 1977 4079 5791 96 Size: 274 80 33 51 1443 2102 1712 138 Fragments arranged by size: 2102 1712 1443 274 138 80 51 33 TstI CACnnnnnnTCCnnnnnnn_nnnnn' Cuts at: 2516 2548 5793 5825 2516 Size: 32 3245 32 2524 Fragments arranged by size: 3245 2524 32 32 Tth111I GACn'n_nGTC Cuts at: 1316 2526 1316 Size: 1210 4623 XbaI T'CTAG_A Cuts at: 2551 3169 2551 Size: 618 5215 XhoI C'TCGA_G Cuts at: 1943 1943 Size: 5833 XmaI C'CCGG_G Cuts at: 1870 3158 1870 Size: 1288 4545 XmnI GAAnn'nnTTC Cuts at: 3962 3962 Size: 5833 ZraI GAC'GTC Cuts at: 276 329 412 598 1534 3641 276 Size: 53 83 186 936 2107 2468 Fragments arranged by size: 2468 2107 936 186 83 53 Enzymes that do cut: AatII AccI Acc65I AclI AcuI AflII AflIII AgeI AhdI AleI AlfI AloI AlwNI ApaLI ApoI AseI AsiSI AvaI BaeI BamHI BanI BanII BbsI BcgI BciVI BclI BglI BglII BlpI Bme1580I BmgBI BmrI BmtI BplI BpmI Bpu10I BpuEI BsaI BsaAI BsaBI BsaHI BsaWI BsaXI BseRI BseYI BsgI BsiEI BsiHKAI BsmI Bsp1286I BspEI BspHI BspMI BsrBI BsrDI BsrFI BsrGI BssHII BssSI BstAPI BstBI BstEII BstYI BtgI BtgZI BtsI ClaI CspCI DraI DrdI EaeI EagI EarI EciI Eco57MI EcoO109I EcoRI FspI HaeII Hin4I HincII HindIII HpaI KpnI MfeI MluI MmeI MscI MslI MspA1I NcoI NdeI NheI NotI NspI PasI PciI PflMI PfoI PmeI PpiI PpuMI PshAI PsiI PstI PvuI SalI SbfI ScaI SfcI SgfI SgrAI SmaI SmlI SnaBI SpeI SphI SspI StuI StyI TaqII TatI TstI Tth111I XbaI XhoI XmaI XmnI ZraI Enzymes that do not cut: AarI AfeI ApaI AscI AvrII BbeI BbvCI BfrBI BsiWI BsmBI BstXI BstZ17I Bsu36I DraIII EcoICRI EcoNI EcoRV FalI FseI FspAI KasI NaeI NarI NgoMIV NruI NsiI PacI PmlI PspOMI PspXI PsrI PvuII RsrII SacI SacII SanDI SapI SexAI SfiI SfoI SrfI SwaI XcmI